Electrochemical monitoring of methyl parathion degradation based on carbon fiber microelectrodes (CFME)

The electrochemical degradation of methyl parathion (MP) has been investigated by using carbon fiber microelectrodes (CFME) as working electrode and acetate buffer pH 5.2 as supporting electrolyte. pnitrophenol (PNP) and p-aminophenol (PAP) recognized as by-products of MP degradation process have been detected and identified in real time using square wave voltammetry. This study shows for the first time that CFME could be used to follow MP degradation in real time and to identify its stables metabolites.Keywords: Organopollutants, decomposition, p-nitrophenol, p-aminophenol, Cyclic voltammetry, Square wave voltammetr

Nosocomial Urinary Infections at the Urogoly Unit of the National University Hospital (Yalgado Ouedraogo), Ouagadougou: Feb.-Sept. 2012

Objective: The aim of this study was to identify the risk factors and the microorganisms susceptibilities of nosocomial urinary infections at the urology unit of the national university hospital of Ouagadougou in Burkina Faso.Method: From February to September 2012, two bacteriological analyzes have been performed for any of the 75 inpatients in the urology unit of the national university hospital of Ouagadougou in Burkina Faso.Results: During the study period, 43 cases of nosocomial urinary infection were identified (57.3%) and we found no statistically significant associated risk factors with age groups, sex, arterial blood pressure, kidney illness and urinary obstructive pathologies.The most frequently isolated bacteria were Escherichia coli (30.9%),  Klebsiella spp (26.9%) and Staphylococcus spp (15.4%). The yeasts strains were very sensitive to antifungal but the bacteria susceptibility rate to antibiotics was very variable. Thus, the cocci were rather sensitive to  association clavulanic acid + amoxicilline and ceftriaxone and enoughsensitive to gentamicine ; the bacilli were enough sensitive to gentamicin and very sensitive to imipenem.Conclusion: From the antibiogram results, we recommend gentamicin in combination with penicillin or metronidazole as the first antibiotics to be used in the treatment of nosocomial urinary tract infections.Keywords: urinary infection, nosocomial infection, bacteria, antibiotic

SWV determination of glyphosate in Burkina Faso soils using carbon fiber microelectrode

This paper presents an evaluation using carbon fiber microelectrode (CFME) for the determination of glyphosate in soils from Burkina Faso treated with Glyphonet SL 360 by square wave voltammetry (SWV).The maximum intensity of the electrochemical response signal of  glyphosate has been optimized and conditions using a 0.2 M of phosphate buffer solution at pH 5.2 as supporting electrolyte and the SWV parameters frequency of 60 Hz, a scan increment of 10 mV and a pulse height of 60 mV. The limit of detection for glyphosate in the Glyphonet SL 360 formulation was 25 ìg L-1 while the quantification limit was 83 ìgL-1with a linear dynamic range up to 50 ìg L-1. In these conditions, a sequence of experiments led to recoveries in the range 88.5 to 102.3% for soil samples spiked with 50, 100 and 200 ìgL-1 of glyphosate with a standard deviation of 11.5, 4.2 and 2.3% respectively indicating the precision of the method. The optimized method was successfully applied to determine the residues of glyphosate in soils collected in the fields in two different dates from the application period.Keywords: Glyphosate, Glyphonet SL 360, carbon fiber microelectrode, square wave voltammetry, sensitivit

Hernie diaphragmatique post-traumatique de l’enfant: à propos d’un cas au Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle de Ouagadougou

La hernie diaphragmatique post-traumatique est une urgence chirurgicale rare chez l’enfant mais pouvant mettre rapidement en jeu le pronosticvital. Les auteurs rapportent le cas d’un garçon de 04 ans admis aux urgences pour douleur abdominale suite à une contusion thoraco-abdominale par accident de la voie publique. Le bilan radiologique initial a consisté en une échographie abdominale qui a révélé un hémopéritoine de petite abondance sans lésion focale. Douze heures après son admission, le patient a présenté une détresse respiratoire avec tableau clinique depneumothorax gauche qui a nécessité une exsufflation en urgence. Le diagnostic de hernie diaphragmatique gauche a été posé à la radiographiedu thorax réalisée après la ponction. L’enfant a bénéficié d’une cure  chirurgicale. L’évolution a été favorable. La hernie diaphragmatique posttraumatique, bien que rare chez l’enfant, devrait être systématiquement recherchée par une radiographie thoracique ou un scanner  thoracoabdominal devant tout traumatisme abdominal avec hyper pression. Son traitement est chirurgical

Sensitive and selective electrochemical analysis of methyl-parathion (MPT) and 4-nitrophenol (PNP) by a new type p-NiTSPc/p-PPD coated carbon fiber microelectrode

Abstract A novel modified carbon fiber microelectrode (CFME) was obtained by combination of tetrasulfonated nickel phtalocyanine (p-NiTSPc) electroformed film associated to para-phenylenediamine (p-PPD) electropolymerized outer-coating. The modified CFMEs where denoted C/p-NiTSPc and C/p-NiTSPc/p-PPD, respectively. These electrodes are dedicated to the organophosphates compounds (OPs) methyl-parathion (MPT) and para-nitrophenol (PNP). Our contribution shows that both OPs can be determined simultaneously on the unmodified and modified C/ p-NiTSPc CFMEs. A clear electrocatalytic activity towards both MPT and PNP redox process was observed, for the first time, in presence of p-NiTSPc. The obtained sensitivity for the C/p-NiTSPc CFME was 80 nA L mg À1 in the concentration range 0.01 to 10 mg/L with a detection limit of 40 mg/L. Also the combination of p-NiTSPc and p-PPD electrodeposited films show, for the first time, the possibility to discriminate on the C/p-NiTSPc/p-PPD CFME between MPT and PNP. Stability experiments were also conducted for 3 weeks in acetate buffer showing a good reproductibility of the sensitivity to PNP vs. time in presence of MPT with a little loss of sensitivity (5%) after 3 weeks

Prevalence of Escherichia coli virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Objective: Diarrhoeagenic E. coli (DEC) strains are important causes of diarrhoea in the developing world and, to a lesser extent, inthe developed world. In this study, we investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from diarrhoeagenic patients in Burkina Faso.Methodology: From September 2016 to Mars 2017, a total of 211 faecal samples from diarrhoeagenic patients from urban hospitals of Ouagadou, Burkina Faso have been analysed. A 16-plex PCR was used to detect simultaneously, the five major DEC pathotypes (enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC)).Results: At least one diarrhoeagenic E. Coli pathotype was detected in 31 samples (14.7%) in children and adults with diarrhoea. EAEC was the most common pathotype detected 9.5% (20/211), followed by EIEC2.4% (05/211) and STEC 0.5% (01/211). More than one DEC pathotype were detected in 2.4% (05/211) patients. EPEC and ETEC were not detected in single infection but in co-infection with others pathotypes.Conclusion: DEC, especially enteroaggregative, may be important responsible of diarrhoeas in Burkina Faso from all ages patient.Key Words: Diarrhoeagenic Escherichia coli, 16-plex PCR, Burkina Faso, human diarrhoeas stool

Microcapteurs électrochimiques pour des mesures durables en Environnement-Santé et Agroalimentaire

National audienc

Dietary patterns of adults living in Ouagadougou and their association with overweight

<p>Abstract</p> <p>Background</p> <p>Urbanization in developing countries comes along with changes in food habits and living conditions and with an increase in overweight and associated health risks. The objective of the study was to describe dietary patterns of adults in Ouagadougou and to study their relationship with anthropometric status of the subjects.</p> <p>Methods</p> <p>A qualitative food frequency questionnaire was administered to 1,072 adults living in two contrasted districts of Ouagadougou. Dietary patterns were defined by principal component analysis and described by multivariate analysis. Logistic regression was used to study their association with overweight.</p> <p>Results</p> <p>The diet was mainly made of cereals, vegetables and fats from vegetable sources. The two first components of the principal component analysis were interpreted respectively as a "snacking" score and as a "modern foods" score. Both scores were positively and independently associated with the economic level of households and with food expenditures (p ≤ 0.001 for both). The "snacking" score was higher for younger people (p = 0.004), for people having a formal occupation (p = 0.006), for those never married (p = 0.005), whereas the "modern foods" score was associated with ethnic group (p = 0.032) and district of residence (p < 0.001). Thirty-six percent of women and 14.5% of men were overweight (Body Mass Index > 25 kg/m²). A higher "modern foods" score was associated with a higher prevalence of overweight when confounding factors were accounted for (OR = 1.19 [95% CI 1.03-1.36]) but there was no relationship between overweight and the "snacking" score.</p> <p>Conclusions</p> <p>Modernisation of types of foods consumed was associated with the living conditions and the environment and with an increased risk of overweight. This should be accounted for to promote better nutrition and prevent non communicable diseases.</p