Regional Differences in Presence of Shiga toxin-producing Escherichia coli Virulence-Associated Genes in the Environment in the North West and East Anglian regions of England

Shiga toxin-producing Escherichia coli is carried in the intestine of ruminant animals, and outbreaks have occurred after contact with ruminant animals or their environment. The presence of STEC virulence genes in the environment was investigated along recreational walking paths in the North West and East Anglia regions of England. In all, 720 boot sock samples from walkers’ shoes were collected between April 2013 and July 2014. Multiplex PCR was used to detect E. coli based on the amplification of the uidA gene and investigate STEC-associated virulence genes eaeA, stx1 and stx2. The eaeA virulence gene was detected in 45·5% of the samples, where stx1 and/or stx2 was detected in 12·4% of samples. There was a difference between the two regions sampled, with the North West exhibiting a higher proportion of positive boot socks for stx compared to East Anglia. In univariate analysis, ground conditions, river flow and temperature were associated with positive boot socks. The detection of stx genes in the soil samples suggests that STEC is present in the English countryside and individuals may be at risk for infection after outdoor activities even if there is no direct contact with animals. Significance and Impact of the Study: Several outbreaks within the UK have highlighted the danger of contracting Shiga toxin-producing Escherichia coli from contact with areas recently vacated by livestock. This is more likely to occur for STEC infections compared to other zoonotic bacteria given the low infectious dose required. While studies have determined the prevalence of STEC within farms and petting zoos, determining the risk to individuals enjoying recreational outdoor activities that occur near where livestock may be present is less researched. This study describes the prevalence with which stx genes, indicative of STEC bacteria, were found in the environment in the English countryside

The Two-hour Marathon: What\u27s the Equivalent for Women?

The principal characteristic of the runner who may break the two-hour barrier in the marathon will be their sex: the person will be male. The fastest men outperform the fastest women because of sex differences in physiology including a higher VȮ2 max. This viewpoint addresses the questions of what is the two-hour equivalent for women, and who will break this barrier? The current sex difference in the world record for the marathon is ~10% which is slightly less than the mean sex difference in performance usually documented between elite men and women distance runners. Based on comparisons of the top 50 marathon times run by men and women, we argue that Paula Radcliffe\u27s world record of 2:15:25 (hr:min:s) set in 2003 is at least equivalent to a two-hour marathon for women. We also provide evidence that there is less depth in elite women\u27s distance running, in part, due to historical and social factors that have led to less opportunity for women than men

Scotland Registry for Ankylosing Spondylitis (SIRAS) – Protocol

Funding SIRAS was funded by unrestricted grants from Pfizer and AbbVie. The project was reviewed by both companies, during the award process, for Scientific merit, to ensure that the design did not compromise patient safety, and to assess the global regulatory implications and any impact on regulatory strategy.Publisher PD

Improved spectral gaps for random quantum circuits Large local dimensions and all to all interactions

Random quantum circuits are a central concept in quantum information theory with applications ranging from demonstrations of quantum computational advantage to descriptions of scrambling in strongly interacting systems and black holes. The utility of random quantum circuits in these settings stems from their ability to rapidly generate quantum pseudorandomness. In a seminal paper by Brand o, Harrow, and Horodecki it was proven that the tth moment operator of local random quantum circuits on n qudits with local dimension q has a spectral gap of at least amp; 937; n amp; 8722;1t amp; 8722;5 amp; 8722;3.1 ln q , which implies that they are efficient constructions of approximate unitary designs. As a first result, we use Knabe bounds for the spectral gaps of frustration free Hamiltonians to show that one dimensional random quantum circuits have a spectral gap scaling as amp; 937; n amp; 8722;1 , provided that t is small compared to the local dimension t2 amp; 8804;O q . This implies a nearly linear scaling of the circuit depth in the design order t. Our second result is an unconditional spectral gap bounded below by amp; 937;[n amp; 8722;1ln amp; 8722;1 n t amp; 8722; amp; 945; q ] for random quantum circuits with all to all interactions. This improves both the n and t scaling in design depth for the nonlocal model. We show this by proving a recursion relation for the spectral gaps involving an auxiliary random walk. Lastly, we solve the smallest nontrivial case exactly and combine with numerics and Knabe bounds to improve the constants involved in the spectral gap for small values of

Restoration of SMN in Schwann cells reverses myelination defects and improves neuromuscular function in spinal muscular atrophy

Spinal muscular atrophy (SMA) is a neuromuscular disease caused by low levels of SMN protein, primarily affecting lower motor neurons. Recent evidence from SMA and related conditions suggests that glial cells can influence disease severity. Here, we investigated the role of glial cells in the peripheral nervous system by creating SMA mice selectively overexpressing SMN in myelinating Schwann cells (Smn(−/−);SMN2(tg/0);SMN1(SC)). Restoration of SMN protein levels restricted solely to Schwann cells reversed myelination defects, significantly improved neuromuscular function and ameliorated neuromuscular junction pathology in SMA mice. However, restoration of SMN in Schwann cells had no impact on motor neuron soma loss from the spinal cord or ongoing systemic and peripheral pathology. This study provides evidence for a defined, intrinsic contribution of glial cells to SMA disease pathogenesis and suggests that therapies designed to include Schwann cells in their target tissues are likely to be required in order to rescue myelination defects and associated disease symptoms

Vibration-tolerant narrow-linewidth semiconductor disk laser using novel frequency-stabilisation schemes

This paper will present developments in narrow-linewidth semiconductor-disk-laser systems using novel frequency-stabilisation schemes for reduced sensitivity to mechanical vibrations, a critical requirement for mobile applications. Narrow-linewidth single-frequency lasers are required for a range of applications including metrology and high-resolution spectroscopy. Stabilisation of the laser was achieved using a monolithic fibre-optic ring resonator with free spectral range of 181 MHz and finesse of 52 to act as passive reference cavity for the laser. Such a cavity can operate over a broad wavelength range and is immune to a wide band of vibrational frequency noise due to its monolithic implementation. The frequency noise of the locked system has been measured and compared to typical Fabry-Perot-locked lasers using vibration equipment to simulate harsh environments, and analysed here. Locked linewidths of < 40 kHz have been achieved. These developments offer a portable, narrow-linewidth laser system for harsh environments that can be flexibly designed for a range of applications

Association Between Early Hyperoxia Exposure After Resuscitation From Cardiac Arrest and Neurological Disability: Prospective Multicenter Protocol-Directed Cohort Study

BACKGROUND: Studies examining the association between hyperoxia exposure after resuscitation from cardiac arrest and clinical outcomes have reported conflicting results. Our objective was to test the hypothesis that early postresuscitation hyperoxia is associated with poor neurological outcome. METHODS: This was a multicenter prospective cohort study. We included adult patients with cardiac arrest who were mechanically ventilated and received targeted temperature management after return of spontaneous circulation. We excluded patients with cardiac arrest caused by trauma or sepsis. Per protocol, partial pressure of arterial oxygen (Pao2) was measured at 1 and 6 hours after return of spontaneous circulation. Hyperoxia was defined as a Pao2 >300 mm Hg during the initial 6 hours after return of spontaneous circulation. The primary outcome was poor neurological function at hospital discharge, defined as a modified Rankin Scale score >3. Multivariable generalized linear regression with a log link was used to test the association between Pao2 and poor neurological outcome. To assess whether there was an association between other supranormal Pao2 levels and poor neurological outcome, we used other Pao2 cut points to define hyperoxia (ie, 100, 150, 200, 250, 350, 400 mm Hg). RESULTS: Of the 280 patients included, 105 (38%) had exposure to hyperoxia. Poor neurological function at hospital discharge occurred in 70% of patients in the entire cohort and in 77% versus 65% among patients with versus without exposure to hyperoxia respectively (absolute risk difference, 12%; 95% confidence interval, 1-23). Hyperoxia was independently associated with poor neurological function (relative risk, 1.23; 95% confidence interval, 1.11-1.35). On multivariable analysis, a 1-hour-longer duration of hyperoxia exposure was associated with a 3% increase in risk of poor neurological outcome (relative risk, 1.03; 95% confidence interval, 1.02-1.05). We found that the association with poor neurological outcome began at ≥300 mm Hg. CONCLUSIONS: Early hyperoxia exposure after resuscitation from cardiac arrest was independently associated with poor neurological function at hospital discharge

Populations of OB-type stars in galaxies

One of the challenges for stellar astrophysics is to reach the point at which we can undertake reliable spectral synthesis of unresolved populations in young, star-forming galaxies at high redshift. Here I summarise recent studies of massive stars in the Galaxy and Magellanic Clouds, which span a range of metallicities commensurate with those in high-redshift systems, thus providing an excellent laboratory in which to study the role of environment on stellar evolution. I also give an overview of observations of luminous supergiants in external galaxies out to a remarkable 6.7 Mpc, in which we can exploit our understanding of stellar evolution to study the chemistry and dynamics of the host systems.Comment: 9 pages, 3 figures, invited review at IAU Symposium 272 (eds Neiner, Wade, Meynet & Peters