The development of linked databases and environmental modelling systems for decision-making in London

A basic requirement for a city's growth is the availability of land, raw material and water. For continued and sustainable development of today’s cities we must be able to meet these basic requirements whilst being mindful of the environment and its relationship with anthropogenic activity. The heterogeneous and complex nature of urban systems where there are obvious environmental and anthropogenic inter-dependencies necessitates a more holistic approach to decision-making. New developments such as linked databases of environmental data and integrated environmental modelling systems provide new ways of organising cross-disciplinary information and a means to apply this to explain, explore and predict the urban systems response to environmental change. In this paper we show how, accessibility to linked databases, detailed understanding of the geology and integrated environmental modelling solutions has the potential to provide decision-makers and policy developers with the science based information needed to understand and address these challenges

From geological complexity to hydrogeological understanding using an integrated 3D conceptual modelling approach : insights from the Cotswolds, UK

Adequate hydrogeological conceptualisation of structurally complex fractured aquifers requires the support of detailed geological mapping and three dimensional understanding. With a geological framework in place uncertainties in hydrological understanding and irregularities in hydraulic observations may be rationalised. Using the Cotswold of southern England, which are underlain by the ooidal limestone-dominated Middle Jurassic Inferior Oolite and Great Oolite groups, 3D modelling software GSI3D and Geographical Information Systems (GIS) have been used to integrate observed hydraulic behaviours with the 3D geological framework. In this way a conceptual model is developed to assist simulation of groundwater flow and the predicted response of groundwater levels and river flows to climatic extremes. The structural and lithological complexity of the bedrock results in sub-catchments which exhibit individual hydraulic responses and a hydrogeological setting dominated by shallow rapid fracture pathways and copious spring discharge

Asking visitors to help: research to guide strategic communication in protected area management

Central to the mission of most protected areas is managing visitor use in such a way that visitor experiences are enhanced while fragile environments are protected. Protected area management agencies annually devote significant financial and human resources to repairing biological and physical damage resulting from uninformed visitor behaviour. Resources do not exist in most agency budgets to provide adequate personnel to monitor and manage visitor behaviour directly, particularly in large or remote areas. To the extent managers are able to harness communication to persuade visitors to voluntarily behave properly in protected areas, significant benefits will accrue both to budgets and to resource protection. Over the long term, millions of dollars that would otherwise be devoted to mitigating visitor-induced impacts could be saved or redirected to other operational priorities such as improving access and visitor services

Symmetry in the insulator - quantum Hall - insulator transitions observed in a Ge/SiGe quantum well

We examine the magnetic field driven insulator-quantum Hall-insulator transitions of the two dimensional hole gas in a Ge/SiGe quantum well. We observe direct transitions between low and high magnetic field insulators and the $\nu=1$ quantum Hall state. With increasing magnetic field, the transitions from insulating to quantum Hall and quantum Hall to insulating are very similar with respect to their transport properties. We address the temperature dependence around the transitions and show that the characteristic energy scale for the high field transition is larger.Comment: 4 page

Poly(ADP-ribosylation) is present in murine sciatic nerve fibers and is altered in a Charcot-Marie-Tooth-1E neurodegenerative model

Background. Poly-ADP-ribose (PAR) is a polymer synthesized by poly-ADP-ribose polymerases (PARPs) as a postranslational protein modification and catabolized mainly by poly-ADP-ribose glycohydrolase (PARG). In spite of the existence of cytoplasmic PARPs and PARG, research has been focused on nuclear PARPs and PAR, demonstrating roles in the maintenance of chromatin architecture and the participation in DNA damage responses and transcriptional regulation. We have recently detected non-nuclear PAR structurally and functionally associated to the E-cadherin rich zonula adherens and the actin cytoskeleton of VERO epithelial cells. Myelinating Schwann cells (SC) are stabilized by E-cadherin rich autotypic adherens junctions (AJ). We wondered whether PAR would map to these regions. Besides, we have demonstrated an altered microfilament pattern in peripheral nerves of Trembler-J (Tr-J) model of CMT1-E. We hypothesized that cytoplasmic PAR would accompany such modified F-actin pattern. Methods. Wild-type (WT) and Tr-J mice sciatic nerves cryosections were subjected to immunohistofluorescence with anti-PAR antibodies (including antibody validation), F-actin detection with a phalloidin probe and DAPI/DNA counterstaining. Confocal image stacks were subjected to a colocalization highlighter and to semi-quantitative image analysis. Results. We have shown for the first time the presence of PAR in sciatic nerves. Cytoplasmic PAR colocalized with F-actin at non-compact myelin regions in WT nerves. Moreover, in Tr-J, cytoplasmic PAR was augmented in close correlation with actin. In addition, nuclear PAR was detected in WT SC and was moderately increased in Tr-J SC. Discussion. The presence of PAR associated to non-compact myelin regions (which constitute E-cadherin rich autotypic AJ /actin anchorage regions) and the co-alterations experienced by PAR and the actin cytoskeleton in epithelium and nerves, suggest that PAR may be a constitutive component of AJ /actin anchorage regions. Is PAR stabilizing the AJ -actin complexes? This question has strong implications in structural cell biology and cell signaling networks. Moreover, if PAR played a stabilizing role, such stabilization could participate in the physiological control of axonal branching. PARP and PAR alterations exist in several neurodegenerative pathologies including Alzheimer's, Parkinson's and Hungtington's diseases. Conversely, PARP inhibition decreases PAR and promotes neurite outgrowth in cortical neurons in vitro. Coherently, the PARP inhibitor XAV939 improves myelination in vitro, ex vivo and in vivo. Until now such results have been interpreted in terms of nuclear PARP activity. Our results indicate for the first time the presence of PARylation in peripheral nerve fibers, in a healthy environment. Besides, we have evidenced a PARylation increase in Tr-J, suggesting that the involvement of cytoplasmic PARPs and PARylation in normal and neurodegenerative conditions should be re-evaluated

Training-induced circuit-specific excitatory synaptogenesis in mice is required for effort control

Synaptogenesis is essential for circuit development; however, it is unknown whether it is critical for the establishment and performance of goal-directed voluntary behaviors. Here, we show that operant conditioning via lever-press for food reward training in mice induces excitatory synapse formation onto a subset of anterior cingulate cortex neurons projecting to the dorsomedial striatum (ACC). Training-induced synaptogenesis is controlled by the Gabapentin/Thrombospondin receptor α2δ−1, which is an essential neuronal protein for proper intracortical excitatory synaptogenesis. Using germline and conditional knockout mice, we found that deletion of α2δ−1 in the adult ACC circuit diminishes training-induced excitatory synaptogenesis. Surprisingly, this manipulation does not impact learning but results in a significant increase in effort exertion without affecting sensitivity to reward value or changing contingencies. Bidirectional optogenetic manipulation of ACC neurons rescues or phenocopies the behaviors of the α2δ−1 cKO mice, highlighting the importance of synaptogenesis within this cortico-striatal circuit in regulating effort exertion.This work was supported by grants from the National Institutes of Health (NS096352 and AG059409 to CE, MH112883 and DA040701 to H.H.Y., MH117429 to I.H.K.). F.P.U.S. was supported by postdoctoral fellowships from the Regeneration Next Initiative and the Ramon y Cajal Young Investigator Award (RYC2021-033202-I). Illustrations were created with BioRender.com. We thank Donna Porter for helping with mouse colony maintenance; Drs. William Wetsel, Staci D. Bilbo, Dolores Irala, Dhanesh Sivadasan Bindu, Alessandro De Simone, and Sehwon Koh for their critical feedback on the manuscript. C.E. is an HHMI Investigator

The reaction of oleic acid monolayers with gas-phase ozone at the air water interface: the effect of sub-phase viscosity, and inert secondary components

Organic films that form on atmospheric particulate matter change the optical and cloud condensation nucleation properties of the particulate matter and consequently have implications for modern climate and climate models. The organic films are subject to attack from gas-phase oxidants present in ambient air. Here we revisit in greater detail the oxidation of a monolayer of oleic acid by gas-phase ozone at the air–water interface as this provides a model system for the oxidation reactions that occur at the air–water interface of aqueous atmospheric aerosol. Experiments were performed on monolayers of oleic acid at the air–liquid interface at atmospherically relevant ozone concentrations to investigate if the viscosity of the sub-phase influences the rate of the reaction and to determine the effect of the presence of a second component within the monolayer, stearic acid, which is generally considered to be non-reactive towards ozone, on the reaction kinetics as determined by neutron reflectometry measurements. Atmospheric aerosol can be extremely viscous. The kinetics of the reaction were found to be independent of the viscosity of the sub-phase below the monolayer over a range of moderate viscosities, Image ID:d0cp03934a-t1.gif, demonstrating no involvement of aqueous sub-phase oxidants in the rate determining step. The kinetics of oxidation of monolayers of pure oleic acid were found to depend on the surface coverage with different behaviour observed above and below a surface coverage of oleic acid of ∼1 × 1018 molecule m−2. Atmospheric aerosol are typically complex mixtures, and the presence of an additional compound in the monolayer that is inert to direct ozone oxidation, stearic acid, did not significantly change the reaction kinetics. It is demonstrated that oleic acid monolayers at the air–water interface do not leave any detectable material at the air–water interface, contradicting the previous work published in this journal which the authors now believe to be erroneous. The combined results presented here indicate that the kinetics, and thus the atmospheric chemical lifetime for unsaturated surface active materials at the air–water interface to loss by reaction with gas-phase ozone, can be considered to be independent of other materials present at either the air–water interface or in the aqueous sub-phase

Using tourism free-choice learning experiences to promote environmentally sustainable behaviour: The role of post-visit ‘action resources’

This paper argues the need for the providers of ecotourism and other free‐choice environmental learning experiences to promote the adoption of environmentally sustainable actions beyond their own sites, when visitors return to their home environments. Previous research indicates that although visitors often leave such experiences with a heightened awareness of conservation issues and intentions to adopt environmentally responsible behaviours, only a minority translate these intentions into real actions. Building on research and theory in relation to visitor experiences in free‐choice learning environments, the paper identifies three different stages in the educational process and proposes a strategy for facilitating the translation of visitors' behavioural intentions into the adoption of sustainable actions through the provision of post‐visit action resources

Long Term Cyclic Pamidronate Reduces Bone Growth by Inhibiting Osteoclast Mediated Cartilage-to-Bone Turnover in the Mouse

Bisphosphonates, used to treat diseases exhibiting increased osteoclast activity, reduce longitudinal bone growth through an as yet undefined mechanism. Pamidronate, an aminobisphosphonate, was given weekly to mice at 0, 1.25, or 2.50 mg/kg/wk beginning at 4 weeks of age. At 12 weeks of age, humeral length, growth plate area, regional chondrocyte cell numbers, chondrocyte apoptosis, TRAP stained osteoclast number, and osteoclast function assessed by cathepsin K immunohistochemistry were quantified. Humeral length was decreased in pamidronate treated mice compared to vehicle control mice, and correlated with greater growth plate areas reflecting greater proliferative and hypertrophic chondrocyte cell numbers with fewer hypertrophic cells undergoing apoptosis. Pamidronate treatment increased TRAP stained osteoclast numbers yet decreased cathepsin K indicating that pamidronate repressed osteoclast maturation and function. The data suggest that long term cyclic pamidronate treatment impairs bone growth by inhibition of osteoclast maturation thereby reducing cartilage-to-bone turnover within the growth plate