In vitro photoprotective activity of plants extracts from Zona da Mata Mineira (Brazil)

Many plants have recently been considered as potential sunscreen resources because of their capacity to absorb the solar ultraviolet radiation. The objective of this work was to evaluate the sunscreen activity of ten Zona da Mata Mineira plants extracts by in vitro test using ultraviolet spectrophotometry. From the studied species, three (Lantana aff. fucata Lindl, Solidago microglossa DC and Stachytarpheta gesnerioides Cham.) showed sun protection factor (SPF) higher than 2, which is the minimum value required by the Brazilian legislation to label a product as containing a SPF. These results suggest that it can be possible to utilize these species to manufacture a new natural sunscreen or associate them with synthetic UV-filters.Colegio de Farmacéuticos de la Provincia de Buenos Aire

In vitro photoprotective activity of plants extracts from Zona da Mata Mineira (Brazil)

Many plants have recently been considered as potential sunscreen resources because of their capacity to absorb the solar ultraviolet radiation. The objective of this work was to evaluate the sunscreen activity of ten Zona da Mata Mineira plants extracts by in vitro test using ultraviolet spectrophotometry. From the studied species, three (Lantana aff. fucata Lindl, Solidago microglossa DC and Stachytarpheta gesnerioides Cham.) showed sun protection factor (SPF) higher than 2, which is the minimum value required by the Brazilian legislation to label a product as containing a SPF. These results suggest that it can be possible to utilize these species to manufacture a new natural sunscreen or associate them with synthetic UV-filters.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

Dentin hypersensitivity is a short, severe pain with fast onset. Therapy aims to either prevent or decrease neural transmission or physically occlude the dentinal tubules. This study aimed to evaluate the effectiveness of commercial desensitizing dentifrice by means of an ex vivo method. Samples (n=8 lower human premolars for each group) were randomly allocated into: G1- brushing with Colgate®Sensitive Pro-Relief; G2- brushing with Sensodyne®Rapid Relief; G3- brushing with Sensodyne®Repair and Protect; and G4- brushing with Colgate®Maximum Cavity Protection. The test bodies were submitted to simulated toothbrushing and dentifrices were analyzed regarding their hydrodynamic size, polydispersity index (PI) and zeta potential. Specimens were evaluated using: scanning electron microscopy (SEM); spectroscopy energy dispersive X-ray (EDS); and profilometry. A qualitative analysis of the photomicrographs and topographies was performed. The dentifrices showed statistical similar physical and chemical characteristics. They also demonstrated obliteration of dentinal tubules when micrographs were observed. Regarding the chemical elements present in the dentin samples, there was a statistically significant difference between the control and experimental surfaces in the four groups. Joint data analysis shows that the desensitizing dentifrice showed better results with regards to the obliteration of dentinal tubules compared to positive and negative controls

In vitro photoprotective activity of plants extracts from Zona da Mata Mineira (Brazil)

Many plants have recently been considered as potential sunscreen resources because of their capacity to absorb the solar ultraviolet radiation. The objective of this work was to evaluate the sunscreen activity of ten Zona da Mata Mineira plants extracts by in vitro test using ultraviolet spectrophotometry. From the studied species, three (Lantana aff. fucata Lindl, Solidago microglossa DC and Stachytarpheta gesnerioides Cham.) showed sun protection factor (SPF) higher than 2, which is the minimum value required by the Brazilian legislation to label a product as containing a SPF. These results suggest that it can be possible to utilize these species to manufacture a new natural sunscreen or associate them with synthetic UV-filters.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Níveis plasmáticos de ácidos graxos e fosfolipase A2 em crianças com autismo

OBJECTIVE: To evaluate fatty acid plasma levels, phospholipase A2 activity, and the developmental profiles of children with autism vs. control subjects. METHODS: Twenty four children with autism underwent laboratory analysis for fatty acid quantification using gas chromatography and PLA2 activity determination by fluorometric assay. RESULTS: No correlation was observed between the developmental quotient and fatty acid plasma levels. Phospholipase A2 activity was significantly higher among autistic children compared with controls. CONCLUSION: The study did not show a correlation between fatty acid and phospholipase A2 plasma levels and the developmental profile of children with autism.OBJETIVO: Avaliar os níveis plasmáticos de ácidos graxos, a atividade da fosfolipase A2 e o perfil de desenvolvimento de crianças com autismo versus controles. MÉTODOS: Vinte e quatro crianças com autismo foram submetidas a exames laboratoriais para quantificação plasmática de ácidos graxos por cromatografia gasosa e para determinação da atividade de fosfolipase A2 por ensaio fluorimétrico. RESULTADOS: Nenhuma correlação foi observada entre o coeficiente de desenvolvimento e os níveis plasmáticos dos ácidos graxos quantificados. A atividade da fosfolipase A2 foi significativamente maior no grupo de crianças com autismo quando comparado ao grupo controle. CONCLUSÃO: O estudo não demonstrou correlação entre os níveis plasmáticos de ácidos graxos e fosfolipase A2 e o perfil de desenvolvimento de crianças com autismo

Mushroom tyrosinase inhibitory activity and major fatty acid constituents of Amazonian native flora oils

Com o intuito de se tratar problemas dermatológicos de hiperpigmentação, há uma tendência mundial no desenvolvimento de cosméticos que possuam propriedades despigmentantes, os quais agem inibindo a biossíntese de melanina. O objetivo deste trabalho foi avaliar in vitro a atividade de inibição da tirosinase de cogumelo de cinco óleos de plantas nativas da Amazônia e, desta forma, verificar a possibilidade de sua incorporação em produtos cosméticos. Ainda, a composição de ácidos graxos dos óleos foi determinada por cromatografia gasosa com detecção por ionização de chama, no intuito de determinar os principais componentes destes óleos. A atividade de inibição da tirosinase dos óleos testados foi encontrada na seguinte ordem: açaí (IA50 = 66,08 µg mL-1) >; tucumã >; patauá >; pracaxi >; castanha do Brasil. Este estudo sugere que o óleo de açaí possui grande potencial para o tratamento da hiperpigmentação cutânea e doenças correlatas, devido à sua considerável atividade de inibição da tirosinase.In order to treat hyperpigmentation-related problems, there has been a global trend in developing cosmetics claiming to have skin-whitening properties, which act by inhibiting melanin biosynthesis. The objective of this work was to evaluate the in vitro mushroom tyrosinase inhibitory activity of five Amazonian native flora oils, and so to verify the possibility of their incorporation into cosmetic products. In addition, the fatty acid composition of the essential oils was determined by gas chromatography-flame ionisation detection in order to determine the main components of these oils. The tyrosinase inhibitory activity of the tested oils was found to be in the following order: açaí (IA50 = 66.08 µg mL-1) >; tucumã >; patauá >; pracaxi >; castanha do Brasil. This study suggests that açaí oil has great potential in the treatment of hyperpigmentation and other related disorders, due to its considerable tyrosinase inhibitory activity

PREVALÊNCIAS DE DOENÇAS DE GRANDE IMPACTO NA SAÚDE PÚBLICA EM IDOSOS FRÁGEIS RESIDENTES NA ZONA DA MATA MINEIRA-BRASIL: UM ESTUDO DE BASE POPULACIONAL

O objetivo deste estudo transversal, descritivo, observacional e de base populacional foi identificar as prevalências de certas doenças de elevado custo e impacto no setor de Saúde Pública e de alguns hábitos de vida de idosos frágeis residentes na zona da Mata Mineira (Brasil). A pesquisa envolveu 7.113 idosos frágeis, de ambos os sexos, com idade igual ou superior a 60 anos. As informações foram obtidas, a partir do banco de dados eletrônico mantido e organizado pelo Centro Mais Vida

In vitro Efficacy of Photoprotection in Sunscreens: a Comparison Between Methods

Sunscreens are known to play an important role on the prevention of skin cancer, as they act by blocking the carcinogenic solar radiations. The currently methods for determining the sun protection efficacy of these products are all based on in vivo tests. In this light, the aim of the present study was to compare the performance of two spectrophotometric in vitro methods, in order to determine which one generates more trustworthy Sun Protection Factors (SPF) values: the classical UV spectrophotometry or the diffuse transmittance reflectance spectrophotometry. For that, twenty-five SPF 30 commercial sunscreen samples were used. The methods generated different results, being the diffuse transmittance spectrophotometry more appropriate and reliable for determining the SPF of these products. Moreover, it provides as additional advantage the possibility of quantifying the protection against UVA radiation through the UVA/UVB Ratio and the Critical Wavelength.Colegio de Farmacéuticos de la Provincia de Buenos Aire

A high performance liquid chromatography method for determination of hydroxyzine hydrochloride in syrup

A simple isocratic method for quantification of hydroxyzine dihydrochloride by HPLC with UV detection at 232 nm in syrup has been developed and validated. Separation was achieved on a C18 column (250 x 4.6 mm, 5 μm) maintained at 90 ºC with 0.5 mol/L potassium dihydrogen phosphate buffer : acetonitrile (1:1, v/v) as mobile phase at a flow rate of 2.0 mL/min. The method was proven to be linear over the range of 80–120 μg/mL, accurate (recovery = 97.8 %), precise (coefficient of variation = 1.52 % for sample) and robust.Colegio de Farmacéuticos de la Provincia de Buenos Aire