Relations Between Stream Chemistry, Fish Diversity, and Land Use in the Upper Little Miami Watershed

Streams are susceptible to numerous threats to their water quality and biodiversity. In southwest Ohio a major driver of these impacts is associated with current agricultural practices and associated legacy effects. These structural and chemical impacts are known to affect biodiversity in these streams. The objective of this study was to document and examine relationships among land-use/land cover, stream chemistry, and fish diversity in the headwaters streams of the Little Miami Watershed. Three streams (Little Miami River, Massies Creek - North Fork, and Massies Creek - South Fork) were sampled in the upper headwaters as well as downstream. Air and water temperature, pH, dissolved oxygen, alkalinity, hardness and turbidity were measured in early September and late November. With the exception of temperature, the measurement of these parameters was generally consistent between sampling periods (CV \u3c 22). A total of 517 fish were collected using an electroshock backpack along 75m stretches at the sites (N = 6). In total 25 species were identified while individual sites yielded 7 - 12 species each. Fish diversity (Simpson’s Diversity, 1 - D) at our sites ranged between 0.77 and 0.86. A significant correlation (r = 0.918, p = 0.01) was found between fish diversity and the % of developed land in these watersheds. Our landscape analysis revealed that all six watersheds had 86 - 97% agricultural land use which had a negative (r = - 0.796), but not statistically significant correlation (p = 0.0581) impact on fish diversity. This work will serve as the baseline for future study of land use impacts on fish diversity, phylogeography, and water quality

The Effects of a Prescribed Burn on Small Mammals in an Ohio Tallgrass Prairie

Prescribed burning is used on prairies for increased prairie health and to manage the communities of organisms that rely on the prairie’s natural resources. Small mammals are also affected by the burning of prairies. Previous research by Chance in 1986 and Cook in 1950 has established that burning decreases the small mammal capture rates in the burned area because of the habitat loss and rapid change of habitat post-burn that stresses the animals and leads to lower reproductive levels (Chance, 1986). Other studies by Francl and Small also showed that small mammal populations did not return to pre-burn numbers even after 16 months of evaluation (Francl, K. E., & Small, C. J., 2013). We hypothesize that small mammal populations will decrease in the prairie after burning because of a lack of habitat and food source and that they will emigrate from the burning site to nearby prairie or similar habitat. We will use baited Sherman live traps placed at 25 meter intervals in order to estimate the various small mammal populations in each of the observed habitats. We will plot the capture locations on a map of the area in order to track how stress affects the shift of population density

High Tunnel Bramble Production in 2008

This report on raspberry and blackberry production was originally published in the 2008 Annual Progress Reports for the Horticulture Research Station (ISRF 0008-36)

Design of MARQUIS2: study protocol for a mentored implementation study of an evidence-based toolkit to improve patient safety through medication reconciliation.

BackgroundThe first Multi-center Medication Reconciliation Quality Improvement Study (MARQUIS1) demonstrated that implementation of a medication reconciliation best practices toolkit decreased total unintentional medication discrepancies in five hospitals. We sought to implement the MARQUIS toolkit in more diverse hospitals, incorporating lessons learned from MARQUIS1.MethodsMARQUIS2 is a pragmatic, mentored implementation QI study which collected clinical and implementation outcomes. Sites implemented a revised toolkit, which included interventions from these domains: 1) best possible medication history (BPMH)-taking; 2) discharge medication reconciliation and patient/caregiver counseling; 3) identifying and defining clinician roles and responsibilities; 4) risk stratification; 5) health information technology improvements; 6) improved access to medication sources; 7) identification and correction of real-time discrepancies; and, 8) stakeholder engagement. Eight hospitalists mentored the sites via one site visit and monthly phone calls over the 18-month intervention period. Each site's local QI team assessed opportunities to improve, implemented at least one of the 17 toolkit components, and accessed a variety of resources (e.g. implementation manual, webinars, and workshops). Outcomes to be assessed will include unintentional medication discrepancies per patient.DiscussionA mentored multi-center medication reconciliation QI initiative using a best practices toolkit was successfully implemented across 18 medical centers. The 18 participating sites varied in size, teaching status, location, and electronic health record (EHR) platform. We introduce barriers to implementation and lessons learned from MARQUIS1, such as the importance of utilizing dedicated, trained medication history takers, simple EHR solutions, clarifying roles and responsibilities, and the input of patients and families when improving medication reconciliation

The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe

The preponderance of matter over antimatter in the early Universe, the dynamics of the supernova bursts that produced the heavy elements necessary for life and whether protons eventually decay --- these mysteries at the forefront of particle physics and astrophysics are key to understanding the early evolution of our Universe, its current state and its eventual fate. The Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed plan for a world-class experiment dedicated to addressing these questions. LBNE is conceived around three central components: (1) a new, high-intensity neutrino source generated from a megawatt-class proton accelerator at Fermi National Accelerator Laboratory, (2) a near neutrino detector just downstream of the source, and (3) a massive liquid argon time-projection chamber deployed as a far detector deep underground at the Sanford Underground Research Facility. This facility, located at the site of the former Homestake Mine in Lead, South Dakota, is approximately 1,300 km from the neutrino source at Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino charge-parity symmetry violation and mass ordering effects. This ambitious yet cost-effective design incorporates scalability and flexibility and can accommodate a variety of upgrades and contributions. With its exceptional combination of experimental configuration, technical capabilities, and potential for transformative discoveries, LBNE promises to be a vital facility for the field of particle physics worldwide, providing physicists from around the globe with opportunities to collaborate in a twenty to thirty year program of exciting science. In this document we provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess.Comment: Major update of previous version. This is the reference document for LBNE science program and current status. Chapters 1, 3, and 9 provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess. 288 pages, 116 figure

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

An algorithm for the extraction of ocean wave spectra from narrow beam HF radar backscatteer

An algorithm is developed and tested to interpret ocean wave spectra from the backscatter return of one or more narrow beam HF radars. The basis of this measurement is the inversion of the integral equation representing the second order radar cross section of the ocean surface (Barrick and Lipa, 1986; Srivastava, 1984; Walsh and Howell, 1990). This equation is numerically inverted by approximating it as a matrix equation and factorizing the resultant kernel matrix using a singular value decomposition to obtain its pseudo-inverse. -- Due to the limitations of the assumption used to linearize the integral equation, the proposed inversion algorithm is best suited for general use at high HF frequencies (≈ 20 to 30 MHz). However, this algorithm may still be applied for the crucial task of monitoring large sea state conditions at even very low HF frequencies (< 10 MHz). -- As a test of this algorithm, comparisons are made between wave spectrum estimates obtained from a WAVEC buoy and a set of two 25.4 MHz ground wave radars that were deployed during the 1986 Canadian Atlantic Storms Program (CASP). Overall, the results of this experiment have been positive and have demonstrated both the basic feasibility of the inversion algorithm and the wave sensing capability of HF radar. -- When using the data of a single radar, the principal information that can be obtained is the nondirectional or one-dimensional (1-D) wave spectrum. Although directional information may be obtained from a single radar it suffers from a left/right directional ambiguity. In general, the comparison of single radar estimates for the 1-D spectrum with those of the buoy at CASP have been good. This is demonstrated by the reasonable average difference from the buoy of ≈ 15% for significant waveheight estimates. This figure is roughly the same for all cross section models. -- When using the data of two radars, not only can more accurate estimates of the 1-D spectrum be obtained but full directional information as well. The comparison of dual-radar wave spectrum estimates with those of the buoy at CASP have been very good. For the Walsh and Howell (1990) cross section model, dual-radar significant waveheight estimates differed from the buoy by only 4.6% on average. For the Barrick and Lipa (1986) and Srivastava (1984) models this average difference is 9.1%. For all models, the average difference for dominant direction estimates is ≈ 10°. -- Although all cross section models produced estimates that correlated well with the buoy, it was the Walsh and Howell (1990) model which consistently provided the best agreement. This would seem to indicate that the Walsh and Howell theory provides a better model for the radar spectrum. Due to the somewhat small size of the CASP data set it is not yet possible to be statistically confident of this finding

Methods for LWIR Radiometric Calibration and Characterization

The utility of a thermal remote sensing system increases with it's ability to retrieve surface temperature or radiance accurately. The radiometer measures the water surface radiant temperature. Combining these measurements with atmospheric pressure, temperature, and water vapor profiles, a top-of-the-atmosphere tradiance estimate can be caluclated with a radiativer transfer code to compare to trhe sensor's output. A novel approach has been developed using an uncooled infrared camera mounted on a boom, to quantify buoy effects