A Metallographic Study Of Solidification And Segregation In Cast Aluminum-uranium Alloys

The micromorphology and type of segregation in vacuum-cast ingots of aluminum-uranium alloys, ranging from 5 to 30 weight percent, were studied. The microstructural characteristics are similar to those of aluminum-silicon alloys of the same composition range, except that the aluminum-uranium eutectic was identified as having chevron and rhombic spiral types of morphology. © 1968

Hyper-Rayleigh scattering in centrosymmetric systems

Hyper-Rayleigh scattering (HRS) is an incoherent mechanism for optical second harmonic generation. The frequency-doubled light that emerges from this mechanism is not emitted in a laser-like manner, in the forward direction; it is scattered in all directions. The underlying theory for this effect involves terms that are quadratic in the incident field and involves an even-order optical susceptibility (for a molecule, its associated hyperpolarizability). In consequence, HRS is often regarded as formally forbidden in centrosymmetric media. However, for the fundamental three-photon interaction, theory based on the standard electric dipole approximation, representable as E13, does not account for all experimental observations. The relevant results emerge upon extending the theory to include E12M1 and E12E2 contributions, incorporating one magnetic dipolar or electric quadrupolar interaction, respectively, to a consistent level of multipolar expansion. Both additional interactions require the deployment of higher orders in the multipole expansion, with the E12E2 interaction analogous in rank and parity to a four-wave susceptibility. To elicit the correct form of response from fluid or disordered media invites a tensor representation which does not oversimplify the molecular components, yet which can produce results to facilitate the interpretation of experimental observations. The detailed derivation in this work leads to results which are summarized for the following: perpendicular detection of polarization components both parallel and perpendicular to the pump radiation, leading to distinct polarization ratio results, as well as a reversal ratio for forward scattered circular polarizations. The results provide a route to handling data with direct physical interpretation, to enable the more sophisticated design of molecules with sought nonlinear optical properties

Laboratory and tentative interstellar detection of trans-methyl formate using the publicly available Green Bank Telescope PRIMOS survey

The rotational spectrum of the higher-energy trans conformational isomer of methyl formate has been assigned for the first time using several pulsed-jet Fourier transform microwave spectrometers in the 6-60 GHz frequency range. This species has also been sought toward the Sagittarius B2(N) molecular cloud using the publicly available PRIMOS survey from the Green Bank Telescope. We detect seven absorption features in the survey that coincide with laboratory transitions of trans-methyl formate, from which we derive a column density of 3.1 (+2.6, -1.2) \times 10^13 cm-2 and a rotational temperature of 7.6 \pm 1.5 K. This excitation temperature is significantly lower than that of the more stable cis conformer in the same source but is consistent with that of other complex molecular species recently detected in Sgr B2(N). The difference in the rotational temperatures of the two conformers suggests that they have different spatial distributions in this source. As the abundance of trans-methyl formate is far higher than would be expected if the cis and trans conformers are in thermodynamic equilibrium, processes that could preferentially form trans-methyl formate in this region are discussed. We also discuss measurements that could be performed to make this detection more certain. This manuscript demonstrates how publicly available broadband radio astronomical surveys of chemically rich molecular clouds can be used in conjunction with laboratory rotational spectroscopy to search for new molecules in the interstellar medium.Comment: 40 pages, 7 figures, 4 tables; accepted for publication in Ap

Preparation and Characterization of Multimodal Hybrid Organic and Inorganic Nanocrystals of Camptothecin and Gold

We demonstrate a novel inorganic-organic crystalline nanoconstruct, where gold atoms were imbedded in the crystal lattices as defects of camptothecin nanocrystals, suggesting its potential use as simultaneous agents for cancer therapy and bioimaging. The incorporation of gold, a potential computed tomography (CT) contrast agent, in the nanocrystals of camptothecin was detected by transmission electron microscope (TEM) and further quantified by energy dispersive X-ray spectrometry (EDS) and inductively coupled plasma-optical emission spectrometers (ICP-OES). Due to gold\u27s high attenuation coefficient, only a relatively small amount needs to be present in order to create a good noise-to-contrast ratio in CT imaging. The imbedded gold atoms and clusters are expected to share the same biological fate as the camptothecin nanocrystals, reaching and accumulating in tumor site due to the enhanced permeation and retention (EPR) effect

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells.

Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene. Despite the inclusion of these elements, the efficacy of these vectors may be limited by transgene silencing due to the genomic environment surrounding the integration site. Barrier insulators can be used to give more consistent expression and resist silencing even with lower vector copies. Here, the barrier activity of an insulator element from the human ankyrin-1 gene was analyzed in a lentiviral vector carrying an antisickling human β-globin gene. Inclusion of a single copy of the Ankyrin insulator did not affect viral titer, and improved the consistency of expression from the vector in murine erythroleukemia cells. The presence of the Ankyrin insulator element did not change transgene expression in human hematopoietic cells in short-term erythroid culture or in vivo in primary murine transplants. However, analysis in secondary recipients showed that the lentiviral vector with the Ankyrin element preserved transgene expression, whereas expression from the vector lacking the Ankyrin insulator decreased in secondary recipients. These studies demonstrate that the Ankyrin insulator may improve long-term β-globin expression in hematopoietic stem cells for gene therapy of hemoglobinopathies

Submillimeter continuum observations of Sagittarius B2 at subarcsecond spatial resolution

We report the first high spatial resolution submillimeter continuum observations of the Sagittarius B2 cloud complex using the Submillimeter Array (SMA). With the subarcsecond resolution provided by the SMA, the two massive star-forming clumps Sgr B2(N) and Sgr B2(M) are resolved into multiple compact sources. In total, twelve submillimeter cores are identified in the Sgr B2(M) region, while only two components are observed in the Sgr B2(N) clump. The gas mass and column density are estimated from the dust continuum emission. We find that most of the cores have gas masses in excess of 100 M$_{\odot}$ and column densities above 10$^{25}$ cm$^{-2}$. The very fragmented appearance of Sgr B2(M), in contrast to the monolithic structure of Sgr B2 (N), suggests that the former is more evolved. The density profile of the Sgr B2(N)-SMA1 core is well fitted by a Plummer density distribution. This would lead one to believe that in the evolutionary sequence of the Sgr B2 cloud complex, a massive star forms first in an homogeneous core, and the rest of the cluster forms subsequently in the then fragmenting structure.Comment: 4 pages, 2 figures, accepted by A&A letter

Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences.

Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral vectors has proven challenging. In this study, we deploy LV-MPRA (lentiviral vector-based, massively parallel reporter assay), an approach that simultaneously analyzes thousands of synthetic DNA fragments in parallel to identify sequence-intrinsic and lineage-specific enhancer function at near-base-pair resolution. We demonstrate the power of LV-MPRA in elucidating the boundaries of previously unknown intrinsic enhancer sequences of the human β-globin locus control region. Our approach facilitated the rapid assembly of novel therapeutic βAS3-globin lentiviral vectors harboring strong lineage-specific recombinant control elements capable of correcting a mouse model of sickle cell disease. LV-MPRA can be used to map any genomic locus for enhancer activity and facilitates the rapid development of therapeutic vectors for treating disorders of the hematopoietic system or other specific tissues and cell types

The structure of molecular clumps around high-mass young stellar objects

We have used the IRAM 30-m and FCRAO 14-m telescopes to observe the molecular clumps associated with 12 ultracompact (UC) HII regions in the J=6-5, 8-7 and 13-12 rotational transitions of methyl-acetylene (CH3C2H). Under the assumption of LTE and optically thin emission, we have derived temperature estimates ranging from 30 to 56 K. We estimate that the clumps have diameters of 0.2-1.6 pc, H_2 densities of 10^5-10^6 {cm^{-3}}, and masses of 10^2-2 10^4 M_\odot. We compare these values with those obtained by other authors from different molecular tracers and find that the H_2 density and the temperature inside the clumps vary respectively like n_{H_2} ~ R^{-2.6} and T ~ R^{-0.5}, with R distance from the centre. We also find that the virial masses of the clumps are ~3 times less than those derived from the CH3C2H column densities: we show that a plausible explanation is that magnetic fields play an important role to stabilise the clumps, which are on the verge of gravitational collapse. Finally, we show that the CH3C2H line width increases for decreasing distance from the clump centre: this effect is consistent with infall in the inner regions of the clumps. We conclude that the clumps around UC HII regions are likely to be transient (~10^(5) yr) entities, remnants of isothermal spheres currently undergoing gravitational collapse: the high mass accretion rates (~10^{-2} M_\odot yr^{-1}) lead to massive star formation at the centre of such clumps.Comment: 15 pages, 11 figures, A & A in pres