519 research outputs found
Domain Organization of Long Autotransporter Signal Sequences
Bacterial autotransporters represent a diverse family of proteins that autonomously translocate across the inner membrane of Gram-negative bacteria via the Sec complex and across the outer bacterial membrane. They often possess exceptionally long N-terminal signal sequences. We analyzed 90 long signal sequences of bacterial autotransporters and members of the two-partner secretion pathway in silico and describe common domain organization found in 79 of these sequences. The domains are in agreement with previously published experimental data. Our algorithmic approach allows for the systematic identification of functionally different domains in long signal sequences
A New Measurement of the Temperature Density Relation of the IGM From Voigt Profile Fitting
We decompose the Lyman-{\alpha} (Ly{\alpha}) forest of an extensive sample of
74 high signal-to-noise ratio and high-resolution quasar spectra into a
collection of Voigt profiles. Absorbers located near caustics in the peculiar
velocity field have the smallest Doppler parameters, resulting in a low-
cutoff in the - set by the thermal state of intergalactic
medium (IGM). We fit this cutoff as a function of redshift over the range
, which allows us to measure the evolution of the IGM
temperature-density () relation parameters
and . We calibrate our measurements against Ly forest
simulations, using 21 different thermal models of the IGM at each redshift,
also allowing for different values of the IGM pressure smoothing scale. We
adopt a forward-modeling approach and self-consistently apply the same
algorithms to both data and simulations, propagating both statistical and
modeling uncertainties via Monte Carlo. The redshift evolution of shows a
suggestive peak at , while our evolution of is consistent with
and disfavors inverted temperature-density relations. Our
measured evolution of and are generally in good agreement with
previous determinations in the literature. Both the peak in the evolution of
at , as well as the high temperatures K
that we observe at , strongly suggest that a significant episode
of heating occurred after the end of HI reionization, which was most likely the
cosmic reionization of HeII.Comment: Accepted for publication in ApJ, 23 pages, 26 figures, machine
readable tables available onlin
On two theorems for flat, affine group schemes over a discrete valuation ring
We include short and elementary proofs of two theorems characterizing
reductive group schemes over a discrete valuation ring, in a slightly more
general context.Comment: 10 pages. To appear in C. E. J.
Evaluation of an Activated Patient Diabetes Education Newsletter
This study evaluated a monthly; activated patient newsletter sent to over 7000 patients in Michigan with diabetes. The newsletter provided concise and action-oriented information about diabetes care. Patients who had signed up to receive the newsletter during the first 4 months of the project (1863) were surveyed to determine how many patients found the newsletter helpful; 80% (1498) of the patients replied. Patients who found the newsletter most helpful were older, had lower incomes, and reported more corrtplications, less understanding of diabetes, and being in poorer overall health. They also were more likely to have non-insulin-dependent diabetes mellitus (NIDDM) than insulin-dependent diabetes mellitus (IDDM). We concluded that the activated patient newsletter is a useful public health/patient education intervention for persons with diabetes. Such a newsletter should be part of a coordinated system of ongoing patient care, education, screening, and social and psychological support.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68904/2/10.1177_014572179402000106.pd
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The structure of blocks with a Klein four defect group
We prove Erdmann’s conjecture [16] stating that every block with a Klein four defect group has a simple module with trivial source, and deduce from this that Puig’s finiteness conjecture holds for source algebras of blocks with a Klein four defect group. The proof uses the classification of finite simple groups
Rank 3 permutation characters and maximal subgroups
In this paper we classify all maximal subgroups M of a nearly simple
primitive rank 3 group G of type L=Omega_{2m+1}(3), m > 3; acting on an L-orbit
E of non-singular points of the natural module for L such that 1_P^G <=1_M^G
where P is a stabilizer of a point in E. This result has an application to the
study of minimal genera of algebraic curves which admit group actions.Comment: 41 pages, to appear in Forum Mathematicu
Combination of tunicamycin with anticancer drugs synergistically enhances their toxicity in multidrug-resistant human ovarian cystadenocarcinoma cells
BACKGROUND: The pharmacologic modulatory effects of the antibiotic, tunicamycin (TM), on multidrug-resistant human UWOV2 ovarian cancer cells are reported. The UWOV2 cell line was derived from a cystadenocarcinoma in a patient refractory to combination chemotherapy with actinomycin D, vincristine (VCR), cis-diaminedichloroplatinum (II) (CDDP) and doxorubicin (DXR). In an attempt to explain drug resistance in this cell line, we examined the effects of TM on their sensitivity to various anticancer drugs, the uptake, efflux and retention of [(3)H]VCR, and their ability to bind [(14)C]DXR and [(3)H]azidopine (AZD), a photoaffinity label of the multidrug transporter, P-glycoprotein (Pgp). RESULTS: TM effectively decreased the EC(50 )for DXR, EXR, VCR and CDDP, thus enhancing their cytotoxicity. The antibiotic also prolonged the intracellular retention time of [(3)H]VCR and increased the binding of both [(14)C]DXR and [(3)H]AZD to the cells. CONCLUSION: It is concluded that the pharmacomodulatory effects of TM in these cells are mediated by global inhibition of protein and glycoprotein synthesis and synergistic interaction with antineoplastic drugs. The ability of TM to enhance the sensitivity of drug resistant tumour cells may have impact on the design and optimization of novel resistance modifiers to improve the efficacy of combination treatment of intractable neoplasms
Platelet - Rich Fibrin (PRF) - The effect of storage time on platelet concentration
The aim of this study was to determine whether storage
time had a significant effect on the platelet concentration of
platelet-rich fibrin (PRF). Three blood samples were drawn
from each participant into a sterile blood sampling tube.
Two of the blood samples were centrifuged to form PRF.
The third non-centrifuged sample was used to measure
the baseline blood platelet concentration. After PRF had
formed, it was removed from the respective test tubes at
different time intervals i.e. immediately after centrifugation
(Group A) and after 60 min of storage time in the blood
collecting tube (Group B). The residual blood from each
group was tested for platelet concentration and compared
with the baseline reading (as an indirect measure of the
platelet concentrate of PRF). The PRF produced in Group
A (PRF A) had a mean platelet concentration of 274
+ - 57.8 X 109/L, whereas the PRF of Group B (PRF B) was
278 + - 58.2 X 109/L. A statistically significant difference was
seen between the groups (p < 0.001).DHE
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