Relevancia de la retroalimentación continua en el proceso de evaluación de desempeño de los asociados de TCS solution center sucursal Colombia

Cuestionario desde la técnica encuesta aplicada para la evaluación de desempeñoEl aporte a las metas y objetivos de la empresa, del área o del proyecto, es difícil de medir sin una correcta evaluación de desempeño, donde se planteen las metas individuales y de equipo y periódicamente se realice el seguimiento objetivo de su avance. Esta es una herramienta, que le permite a las empresas medir el aporte individual, grupal, por grados o roles y así mismo dar feedback a los colaboradores ayudándolos a encaminar sus esfuerzos hacia los objetivos corporativos o del negocio, reconocer sus oportunidades de mejora y lograr su optimo desempeño. Es un tema que va ligado a la formación y el desarrollo y al programa de reconocimientos, estas dos funciones son complemento, para que la evaluación de desempeño tenga buenos resultados. Por lo que el desarrollo de esta investigación está encaminada a identificar las diversas condiciones de la evaluación de desempeño desarrollada en la organización TCS Solution center sucursal Colombia, con el propósito de proponer acciones de mejora en el proceso de retroalimentación de los resultados de la evaluación de desempeño que podrían redundar en el aumento de la productividad y satisfacción en los trabajadores de la organización.The contribution to the goals and objectives of the company, the area or the project is difficult to measure without a correct performance evaluation, where individual and team goals are set and periodically objective monitoring of their progress is carried out. This is a tool that allows companies to measure individual, group, grade or role contributions and also give feedback to employees, helping them direct their efforts towards corporate or business objectives, recognize their opportunities for improvement and achieve its optimal performance. It is an issue that is linked to training and development and the recognition program, these two functions are complementary, so that the performance evaluation has good results. Therefore, the development of this research is aimed at identifying the various conditions of the performance evaluation developed in the organization TCS Solution center Colombia branch, with the purpose of proposing improvement actions in the feedback process of the results of the evaluation of performance. performance that could result in increased productivity and satisfaction in the organization's workers

Simultaneous Isolation of Circulating Nucleic Acids and EV-Associated Protein Biomarkers From Unprocessed Plasma Using an AC Electrokinetics-Based Platform.

The power of personalized medicine is based on a deep understanding of cellular and molecular processes underlying disease pathogenesis. Accurately characterizing and analyzing connections between these processes is dependent on our ability to access multiple classes of biomarkers (DNA, RNA, and proteins)-ideally, in a minimally processed state. Here, we characterize a biomarker isolation platform that enables simultaneous isolation and on-chip detection of cell-free DNA (cfDNA), extracellular vesicle RNA (EV-RNA), and EV-associated proteins in unprocessed biological fluids using AC Electrokinetics (ACE). Human biofluid samples were flowed over the ACE microelectrode array (ACE chip) on the Verita platform while an electrical signal was applied, inducing a field that reversibly captured biomarkers onto the microelectrode array. Isolated cfDNA, EV-RNA, and EV-associated proteins were visualized directly on the chip using DNA and RNA specific dyes or antigen-specific, directly conjugated antibodies (CD63, TSG101, PD-L1, GPC-1), respectively. Isolated material was also eluted off the chip and analyzed downstream by multiple methods, including PCR, RT-PCR, next-generation sequencing (NGS), capillary electrophoresis, and nanoparticle size characterization. The detection workflow confirmed the capture of cfDNA, EV-RNA, and EV-associated proteins from human biofluids on the ACE chip. Tumor specific variants and the mRNAs of housekeeping gene PGK1 were detected in cfDNA and RNA isolated directly from chips in PCR, NGS, and RT-PCR assays, demonstrating that high-quality material can be isolated from donor samples using the isolation workflow. Detection of the luminal membrane protein TSG101 with antibodies depended on membrane permeabilization, consistent with the presence of vesicles on the chip. Protein, morphological, and size characterization revealed that these vesicles had the characteristics of EVs. The results demonstrated that unprocessed cfDNA, EV-RNA, and EV-associated proteins can be isolated and simultaneously fluorescently analyzed on the ACE chip. The compatibility with established downstream technologies may also allow the use of the platform as a sample preparation method for workflows that could benefit from access to unprocessed exosomal, genomic, and proteomic biomarkers

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Review of the decisions of the contentious administrative jurisdiction by the international jurisprudence

Teniendo en cuenta el fenómeno de la Constitucionalización del Derecho, en este caso del Derecho Administrativo y en especial la apertura y subordinación del ordenamiento jurídico colombiano al Derecho Internacional de los Derechos Humanos, se hace imperante, tal y como ya se hizo en el Derecho Penal. Permitir que las decisiones de los organismos internacionales de Derechos Humanos se conviertan en causal del recurso extraordinario de revisión del Código Contencioso Administrativo, con el fin de cumplir con lo que se conoce como reparación a las violaciones a los Derechos Humanos.Pursuant to the constitutional law phenomenon, and regarding to the administrative law, specially, the opening and subordination of the national law towards the international human rights law. It is imperative that as previously done in the criminal law, to allow that the decisions coming from the human rights international organizations could be ground within the review of the extraordinary remedies, of the contentious administrative code, with the intention to serve to the term known as reparation inside the human rights violations

La revisión de las decisiones del contencioso administrativo por parte de las sentencias internacionales

Teniendo en cuenta el fenómeno de la Constitucionalización del Derecho, en este caso del Derecho Administrativo y en especial la apertura y subordinación del ordenamiento jurídico colombiano al Derecho Internacional de los Derechos Humanos, se hace imperante, tal y como ya se hizo en el Derecho Penal. Permitir que las decisiones de los organismos internacionales de Derechos Humanos se conviertan en causal del recurso extraordinario de revisión del Código Contencioso Administrativo, con el fin de cumplir con lo que se conoce como reparación a las violaciones a los Derechos Humanos

Review of the decisions of the contentious administrative jurisdiction by the international jurisprudence

Teniendo en cuenta el fenómeno de la Constitucionalización del Derecho, en este caso del Derecho Administrativo y en especial la apertura y subordinación del ordenamiento jurídico colombiano al Derecho Internacional de los Derechos Humanos, se hace imperante, tal y como ya se hizo en el Derecho Penal. Permitir que las decisiones de los organismos internacionales de Derechos Humanos se conviertan en causal del recurso extraordinario de revisión del Código Contencioso Administrativo, con el fin de cumplir con lo que se conoce como reparación a las violaciones a los Derechos Humanos.Pursuant to the constitutional law phenomenon, and regarding to the administrative law, specially, the opening and subordination of the national law towards the international human rights law. It is imperative that as previously done in the criminal law, to allow that the decisions coming from the human rights international organizations could be ground within the review of the extraordinary remedies, of the contentious administrative code, with the intention to serve to the term known as reparation inside the human rights violations

Educación para la paz: Instrumento esencial en la gestión del postconflicto colombiano

The purpose of this paper consists in review the Peace Education definition from the legal perspective that involves the analysis of national and international law in Colombia related to this topic.El objeto de este artículo es revisar el concepto de Educación para la Paz, desde una perspectiva jurídica, abarcando la normativa colombiana y la internacional con miras a realizar algunas consideraciones de orden práctico y jurídico

Neutron Reflectivity Study on the Postpolymerization Modification of Poly(2-hydroxyethyl methacrylate) Brushes

Postpolymerization modification reactions are widely employed to prepare functional polymer brushes. Relatively little is known, however, about the distribution of functional groups in such postmodified brushes. Using neutron reflectometry and UV-vis spectroscopy as principal tools, this article investigates the p-nitrophenyl chloroformate (NPC)-mediated postpolymerization modification of poly(2-hydroxyethyl methacrylate) (PHEMA) brushes, prepared via surface-initiated atom transfer radical polymerization, with D-10 leucine and D-3 serine. The neutron reflectometry experiments indicate that the postpolymerization modification depends both on brush thickness and grafting density. Whereas for dense brushes, postpolymerization modification with D-10 leucine is limited to the top similar to 200 angstrom of the brush, independently of the brush thickness, the extent of postmodification can be significantly enhanced by decreasing the grafting density of the brush or by using the more hydrophilic and sterically less demanding D-3 serine, which reflects the ability of this amino acid to more readily penetrate the brush. UV-vis experiments revealed that the NPC activation is also nonuniform, but brush thickness and grafting density dependent, which adds to brush thickness and density and the nature of the amino acid as another of a complex set of variables that determine the final distribution of functional groups in postmodified brushes