Soybean Aphid: A New Pest for North America

The soybean aphid (Aphis glycines), originally a native of China and Japan, was first identified in the United States during the summer and fall of 2000. Infestations were confirmed in several Midwestern states from Ohio to West Virginia and Kentucky, west into Missouri and Iowa. Late last summer, the heaviest infestations were located in Michigan, Wisconsin, Minnesota, and Illinois. At the moment, we do not know of confirmed infestations within the state of Kansas, with the nearest verified sightings occurring about midway through Iowa and Missouri.Originating text in English.Citation: Higgins, R. (2002). Soybean Aphid: A New Pest for North America. Kansas State University, Research and Extension. https://web.archive.org/web/20011125111927/http://www.msue.msu.edu/ipm/CAT01_field/FC07-12-01.ht

TurboExpander Wheel Damage Analysis: A Case Study

Case StudyCS8Damage analysis of a turbo-expander wheel in a geothermal application will be discussed. Presentation includes: background, description of damage, damage analysis, conclusions, and finally discussion of remedial actions to achieve longer life for the wheel

Unfair Treatment and Self-Regulatory Focus

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/89950/1/oyserman__uskul__yoder__et_al..pd

Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses

Xenotransplantation (cross-species transplantation) using genetically-engineered pig organs offers a potential solution to address persistent organ shortage. Current evaluation of porcine genetic modifications is to monitor the nonhuman primate immune response and survival after pig organ xenotransplantation. This measure is an essential step before clinical xenotransplantation trials, but it is time-consuming, costly, and inefficient with many variables. We developed an efficient approach to quickly examine human-to-pig xeno-immune responses in vitro. A porcine endothelial cell was characterized and immortalized for genetic modification. Five genes including GGTA1, CMAH, β4galNT2, SLA-I α chain, and β2-microglobulin that are responsible for the production of major xenoantigens (αGal, Neu5Gc, Sda, and SLA-I) were sequentially disrupted in immortalized porcine endothelial cells using CRISPR/Cas9 technology. The elimination of αGal, Neu5Gc, Sda, and SLA-I dramatically reduced the antigenicity of the porcine cells, though the cells still retained their ability to provoke human natural killer cell activation. In summary, evaluation of human immune responses to genetically modified porcine cells in vitro provides an efficient method to identify ideal combinations of genetic modifications for improving pig-to-human compatibility, which should accelerate the application of xenotransplantation to humans

Strategy for wildlife ecological risk assessment at Teck Cominco’s Kimberley operations, BC

The overall objective of this predictive wildlife ecological risk assessment (ERA) is to assess contaminant-related risks after implementation of Teck Cominco's closure plan for its Kimberley Operations. The first stage of the wildlife ERA, problem formulation (PF), was conducted to identify the most likely sources of potential risks for the site to help focus subsequent ERA investigations. This paper summarizes key findings of the PF and presents an overview of the main components of the wildlife ERA. A separate paper (Mann et al., 2004) addresses potential risks to the aquatic environment.Non UBCUnreviewedOthe

Strategy for aquatic ecological risk assessment at Teck Cominco's Kimberley operations, BC

The overall objective of this predictive aquatic ecological risk assessment (ERA) is to assess contaminant-related risks after implementation of Teck Cominco’s closure plan for the site. The first stage of the ERA, problem formulation (PF), was conducted to identify the most likely risk drivers for the site to help focus subsequent aquatic ERA investigations. This paper summarizes the key findings of the PF and presents an overview of the main components of the aquatic ERA. The terrestrial component of the ERA is presented in a separate paper (Allard et al., 2004).Non UBCUnreviewedOthe

Effects of Lymphocyte Isolation and Timing of Processing on Detection of CD127 Expression on T Cells in Human Immunodeficiency Virus-Infected Patients

Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials

Effects of Delays in Peripheral Blood Processing, Including Cryopreservation, on Detection of CD31 Expression on Naïve CD4 T Cells▿

Delayed processing of peripheral blood or peripheral blood mononuclear cell isolation and cryopreservation can lead to the detection of somewhat higher levels of CD31 expression on naïve CD4 T cells by flow cytometry. These observations should be considered in the planning of multicenter clinical trials and in the interpretation of the results of functional studies