Relative Humidity on Mars: New Results From the Phoenix TECP Sensor

In situ measurements of relative humidity (RH) on Mars have only been performed by the Phoenix (PHX) and Mars Science Laboratory (MSL) missions. Here we present results of our recalibration of the PHX thermal and electrical conductivity probe (TECP) RH sensor. This recalibration was conducted using a TECP engineering model subjected to the full range of environmental conditions at the PHX landing site in the Michigan Mars Environmental Chamber. The experiments focused on the warmest and driest conditions (daytime) because they were not covered in the original calibration (Zent et al., 2010, https://doi.org/10.1029/2009JE003420) and previous recalibration (Zent et al., 2016, https://doi.org/10.1002/2015JE004933). In nighttime conditions, our results are in excellent agreement with the previous 2016 recalibration, while in daytime conditions, our results show larger water vapor pressure values. We obtain vapor pressure values in the range ~0.005–1.4 Pa, while Zent et al. (2016, https://doi.org/10.1002/2015JE004933) obtain values in the range ~0.004–0.4 Pa. Our higher daytime values are in better agreement with independent estimates from the ground by the PHX Surface Stereo Imager instrument and from orbit by Compact Reconnaissance Imaging Spectrometer for Mars. Our results imply larger day‐to‐night ratios of water vapor pressure at PHX compared to MSL, suggesting a stronger atmosphere‐regolith interchange in the Martian arctic than at lower latitudes. Further, they indicate that brine formation at the PHX landing site via deliquescence can be achieved only temporarily between midnight and 6 a.m. on a few sols. The results from our recalibration are important because they shed light on the near‐surface humidity environment on Mars.Key PointsWe have recalibrated the relative humidity sensor of the Mars Phoenix landerWe obtain water vapor pressure values in the range ~0.005–1.4 Pa, while in previous recalibrations, values in the range ~0.004–0.4 PaOur results show a two‐order‐of‐magnitude diurnal variation of water vapor pressure, suggesting a strong atmosphere‐regolith interchangePlain Language SummaryWe present our recalibration of Phoenix’s humidity sensor. This recalibration was conducted with a copy of the sensor subjected to the environmental conditions at the Phoenix landing site. Our experiments focus on the warmest and driest conditions because they were not covered in previous calibrations. Our recalibration shows daytime water content values one order of magnitude larger than those in the previous calibration. At nighttime conditions, our results are in excellent agreement with the previous calibration. Our higher daytime values are in better agreement with independent estimates from the ground, and from orbit. Our results imply larger diurnal variations of water content at Phoenix compared to Curiosity, suggesting a stronger atmosphere‐soil interchange in the Martian arctic than at lower latitudes. Further, they indicate that environmental conditions favorable for the formation of saline solutions (brine) are only achieved temporarily between midnight and 6 a.m. on a few Martian days. The results from our recalibration are important because measurements of humidity on the Martian surface are needed to shed light on the local and global water cycle of Mars, and so far, only the Phoenix mission in the arctic region and the Curiosity rover at equatorial latitudes have performed such measurements.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153252/1/jgre21230.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/153252/2/jgre21230_am.pd

Differences in collagen prolyl 4-hydroxylase assembly between two Caenorhabditis nematode species despite high amino acid sequence identity of the enzyme subunits

The collagen prolyl 4-hydroxylases (P4Hs) are essential for proper extracellular matrix formation in multicellular organisms. The vertebrate enzymes are α2β2 tetramers, in which the β subunits are identical to protein disulfide isomerase (PDI). Unique P4H forms have been shown to assemble from the <i>Caenorhabditis</i> <i>elegans</i> catalytic α subunit isoforms PHY-1 and PHY-2 and the β subunit PDI-2. A mixed PHY-1/PHY-2/(PDI-2)<sub>2</sub> tetramer is the major form, while PHY-1/PDI- 2 and PHY-2/PDI-2 dimers are also assembled but less efficiently. Cloning and characterization of the orthologous subunits from the closely related nematode <i>Caenorhabditis</i> <i>briggsae</i> revealed distinct differences in the assembly of active P4H forms in spite of the extremely high amino acid sequence identity (92-97%) between the <i>C. briggsae</i> and <i>C. elegans</i> subunits. In addition to a PHY-1/PHY-2(PDI-2)<sub>2</sub> tetramer and a PHY-1/PDI-2 dimer, an active (PHY- 2)<sub>2</sub>(PDI-2)<sub>2</sub> tetramer was formed in <i>C. briggsae</i> instead of a PHY-2/PDI-2 dimer. Site-directed mutagenesis studies and generation of inter-species hybrid polypeptides showed that the N-terminal halves of the <i>Caenorhabditis</i> PHY-2 polypeptides determine their assembly properties. Genetic disruption of <i>C. briggsae phy-1</i> (<i>Cb-dpy-18</i>) via a <i>Mos1</i> insertion resulted a small (short) phenotype that is less severe than the dumpy (short and fat) phenotype of the corresponding <i>C. elegans</i> mutants (<i>Ce-dpy-18</i>). <i>C. briggsae</i> <i>phy-2</i> RNA interference produced no visible phenotype in the wild type nematodes but produced a severe dumpy phenotype and larval arrest in <i>phy-1</i> mutants. Genetic complementation of the <i>C. briggsae</i> and <i>C. elegans</i> <i>phy-1</i> mutants was achieved by injection of a wild type <i>phy-1</i> gene from either species

COVID-19 outbreak at a reception centre for asylum seekers in Espoo, Finland

Publisher Copyright: © 2021Background shared accommodation may increase the risk of SARS-CoV-2 transmission. In April 2020, an increasing number of asylum seekers at a reception centre in Espoo, Finland presented with COVID-19 despite earlier implementation of preventive measures. We decided to screen the entire population of the centre for SARS-CoV-2. Methods we offered nasopharyngeal swab collection and SARS-CoV-2 real-time polymerase chain reaction (RT-PCR) analysis to the centre's clients. Symptoms were recorded at the time of diagnostic sample collection using electronic forms and followed up for two weeks through phone interviews and a review of medical records. Findings 260 clients were screened. Of them, 96 (37%) were found positive for SARS-CoV-2 and isolated. The high attack rate prompted the local public health authority to set the other clients in quarantine for 14 days to prevent further spread. Of the positive cases, 61 (64%) reported having had symptoms at the time of the screening or one week prior. Of the 35 initially asymptomatic individuals, 12 developed symptoms during follow-up, while 23 (or 18% of all screened SARS-CoV-2 positive clients) remained asymptomatic. No widespread transmission of COVID-19 was detected after the quarantine was lifted. Interpretation in this large COVID-19 outbreak, voluntary mass screening provided valuable information about its extent and helped guide the public health response. Comprehensive quarantine and isolation measures were likely instrumental in containing the outbreak. Funding Finnish Institution for Health and Welfare, Finnish Immigration Agency, City of EspooPeer reviewe

Expression profiling of metalloproteinases and tissue inhibitors of metalloproteinases in normal and degenerate human achilles tendon

To profile the messenger RNA (mRNA) expression for the 23 known genes of matrix metalloproteinases (MMPs), 19 genes of ADAMTS, 4 genes of tissue inhibitors of metalloproteinases (TIMPs), and ADAM genes 8, 10, 12, and 17 in normal, painful, and ruptured Achilles tendons. Tendon samples were obtained from cadavers or from patients undergoing surgical procedures to treat chronic painful tendinopathy or ruptured tendon. Total RNA was extracted and mRNA expression was analyzed by quantitative real-time reverse transcription–polymerase chain reaction, normalized to 18S ribosomal RNA. In comparing expression of all genes, the normal, painful, and ruptured Achilles tendon groups each had a distinct mRNA expression signature. Three mRNA were not detected and 14 showed no significant difference in expression levels between the groups. Statistically significant (P < 0.05) differences in mRNA expression, when adjusted for age, included lower levels of MMPs 3 and 10 and TIMP-3 and higher levels of ADAM-12 and MMP-23 in painful compared with normal tendons, and lower levels of MMPs 3 and 7 and TIMPs 2, 3, and 4 and higher levels of ADAMs 8 and 12, MMPs 1, 9, 19, and 25, and TIMP-1 in ruptured compared with normal tendons. The distinct mRNA profile of each tendon group suggests differences in extracellular proteolytic activity, which would affect the production and remodeling of the tendon extracellular matrix. Some proteolytic activities are implicated in the maintenance of normal tendon, while chronically painful tendons and ruptured tendons are shown to be distinct groups. These data will provide a foundation for further study of the role and activity of many of these enzymes that underlie the pathologic processes in the tendon

Humidity calibration of relative humidity devices in Martian conditions

Finnish Meteorological Institute (FMI) has developed relative humidity measurement devices for past and future Mars lander missions: REMS-H for Curiosity, MEDA HS for Mars 2020 and METEO-H for ExoMars 2020. The sensors used in these devices are HUMICAP® capacitive thin-film polymer sensors by Vaisala Inc. New calibration measurements are performed with ground reference models of these devices in the Mars Simulation Facility (MSF) and Planetary Analog Simulation Laboratory (PASLAB) at the German Aerospace Center (DLR) in spring 2020. The preliminary results will be given at the EGU 2020. Calibration of relative humidity devices requires in minimum two humidity points over the expected operational temperature and pressure range of the device. With two-point calibration the relative humidity devices can be used for scientific measurements with satisfactory quality but the uncertainty is notable. Stable humidity conditions between dry and saturation humidity in Martian conditions can be achieved reliably in very few laboratories in the whole world and humidity measurements in Martian conditions have been previously performed for the same devices in FMI laboratory and in Michigan Mars Environmental Chamber (MMEC) at the University of Michigan. The new measurement campaign will consist of stable humidity point measurements in multiple temperatures between +10°C to -70°C in CO2 gas and Martian pressure of approximately 7 hPa. The measurements are performed simultaneously for multiple devices in a small pressure vessel with continuous humidified carbon dioxide flow. The new measurement campaign will improve the characterization of the existing relative humidity devices in Mars lander missions and define in more detail the measurement uncertainties

Calibration and first results of relative humidity sensor MEDA HS onboard M2020 rover

MEDA HS is the relative humidity sensor on the Mars 2020 Perseverance rover provided by theFinnish Meteorological Institute (FMI). The sensor is a part of Mars Environmental DynamicAnalyzer (MEDA), a suite of environmental sensors provided by Centro de Astrobiología in Madrid,Spain. MEDA HS, along with METEO-H in ExoMars 2022 surface platform, is a successor of REMS-Hon board Curiosity.Calibration of relative humidity (RH) instruments for Mars missions is challenging due to the rangeof RH (from 0 to close to 100%) and temperature conditions (from about -90 ºC to + 22 ºC) thatneed to be simulated in the lab. Thermal gradients in different parts of the system need to be wellknown and controlled to ensure reliable reference RH readings. For MEDA HS the calibration testshave been performed for different models of MEDA HS in three Martian humidity simulatorlaboratories: FMI laboratory, Michigan Mars Environmental Chamber (MMEC) and DLR PASLAB(Planetary Analog Simulation Laboratory). MEDA HS flight model was tested at FMI together with flight spare and ground reference models inlow pressure dry CO2 gas from +22ºC to -70ºC and in saturation conditions from -40ºC down to-70ºC. Further, the MEDA HS flight model final calibration is complemented by calibration datatransferred from an identical ground reference model which has gone through rigorous testingalso after the flight model delivery. During the test campaign at DLR PASLAB that started inAutumn 2020, MEDA HS has been calibrated over the full relative humidity scale between -70 to-40ºC in CO2 in the pressure ranges from 5.5 to 9.5 hPa, representative of Martian surfaceatmospheric pressure. The results can be extrapolated to higher and lower temperatures.In this presentation the final flight calibration and performance of the MEDA HS will be presentedtogether with first results expected from the surface of Mars by the Perseverance rover

Gene Ontology annotations and resources.

The Gene Ontology (GO) Consortium (GOC, http://www.geneontology.org) is a community-based bioinformatics resource that classifies gene product function through the use of structured, controlled vocabularies. Over the past year, the GOC has implemented several processes to increase the quantity, quality and specificity of GO annotations. First, the number of manual, literature-based annotations has grown at an increasing rate. Second, as a result of a new 'phylogenetic annotation' process, manually reviewed, homology-based annotations are becoming available for a broad range of species. Third, the quality of GO annotations has been improved through a streamlined process for, and automated quality checks of, GO annotations deposited by different annotation groups. Fourth, the consistency and correctness of the ontology itself has increased by using automated reasoning tools. Finally, the GO has been expanded not only to cover new areas of biology through focused interaction with experts, but also to capture greater specificity in all areas of the ontology using tools for adding new combinatorial terms. The GOC works closely with other ontology developers to support integrated use of terminologies. The GOC supports its user community through the use of e-mail lists, social media and web-based resources

An expanded evaluation of protein function prediction methods shows an improvement in accuracy

Background: A major bottleneck in our understanding of the molecular underpinnings of life is the assignment of function to proteins. While molecular experiments provide the most reliable annotation of proteins, their relatively low throughput and restricted purview have led to an increasing role for computational function prediction. However, assessing methods for protein function prediction and tracking progress in the field remain challenging. Results: We conducted the second critical assessment of functional annotation (CAFA), a timed challenge to assess computational methods that automatically assign protein function. We evaluated 126 methods from 56 research groups for their ability to predict biological functions using Gene Ontology and gene-disease associations using Human Phenotype Ontology on a set of 3681 proteins from 18 species. CAFA2 featured expanded analysis compared with CAFA1, with regards to data set size, variety, and assessment metrics. To review progress in the field, the analysis compared the best methods from CAFA1 to those of CAFA2. Conclusions: The top-performing methods in CAFA2 outperformed those from CAFA1. This increased accuracy can be attributed to a combination of the growing number of experimental annotations and improved methods for function prediction. The assessment also revealed that the definition of top-performing algorithms is ontology specific, that different performance metrics can be used to probe the nature of accurate predictions, and the relative diversity of predictions in the biological process and human phenotype ontologies. While there was methodological improvement between CAFA1 and CAFA2, the interpretation of results and usefulness of individual methods remain context-dependent. Keywords: Protein function prediction, Disease gene prioritizationpublishedVersio

An Expanded Evaluation of Protein Function Prediction Methods Shows an Improvement In Accuracy

Background: A major bottleneck in our understanding of the molecular underpinnings of life is the assignment of function to proteins. While molecular experiments provide the most reliable annotation of proteins, their relatively low throughput and restricted purview have led to an increasing role for computational function prediction. However, assessing methods for protein function prediction and tracking progress in the field remain challenging. Results: We conducted the second critical assessment of functional annotation (CAFA), a timed challenge to assess computational methods that automatically assign protein function. We evaluated 126 methods from 56 research groups for their ability to predict biological functions using Gene Ontology and gene-disease associations using Human Phenotype Ontology on a set of 3681 proteins from 18 species. CAFA2 featured expanded analysis compared with CAFA1, with regards to data set size, variety, and assessment metrics. To review progress in the field, the analysis compared the best methods from CAFA1 to those of CAFA2. Conclusions: The top-performing methods in CAFA2 outperformed those from CAFA1. This increased accuracy can be attributed to a combination of the growing number of experimental annotations and improved methods for function prediction. The assessment also revealed that the definition of top-performing algorithms is ontology specific, that different performance metrics can be used to probe the nature of accurate predictions, and the relative diversity of predictions in the biological process and human phenotype ontologies. While there was methodological improvement between CAFA1 and CAFA2, the interpretation of results and usefulness of individual methods remain context-dependent