Analysis of genome expression of rhizobium etli in free living and plant-associated symbiotic state

Comunicaciones a congreso

Reported Dietary Intake, Disparity between the Reported Consumption and the Level Needed for Adequacy and Food Sources of Calcium, Phosphorus, Magnesium and Vitamin D in the Spanish Population: Findings from the ANIBES Study

Calcium, phosphorus, magnesium and vitamin D have important biological roles in the body, especially in bone metabolism. We aimed to study the reported intake, the disparity between the reported consumption and the level needed for adequacy and food sources of these four nutrients in the Spanish population. We assessed the reported intake for both, general population and plausible reporters. Results were extracted from the ANIBES survey, n = 2009. Three-day dietary reported intake data were obtained and misreporting was assessed according to the European Food Safety Authority (EFSA). Mean ± SEM (range) total reported consumption of calcium, phosphorus, magnesium, and vitamin D for the whole population were 698 ± 7 mg/day (71–2551 mg/day), 1176 ± 8 mg/day, (331–4429 mg/day), 222 ± 2 mg/day (73–782 mg/day), and 4.4 ± 0.1 µg/day (0.0–74.2 µg/day), respectively. In the whole group, 76% and 66%; 79% and 72%; and 94% and 93% of the population had reported intakes below 80% of the national and European recommended daily intakes for calcium, magnesium and vitamin D, respectively; these percentages were over 40% when the plausible reporters were analysed separately. The main food sources were milk and dairy products for calcium and phosphorus, cereals and grains for magnesium and fish for vitamin D. In conclusion, there is an important percentage of the Spanish ANIBES population not meeting the recommended intakes for calcium, magnesium and vitamin D.The ANIBES study was financially supported by a grant from Coca-Cola Iberia through an agreement with the Spanish Nutrition Foundation (FEN)

Dietary sources and intakes of folates and vitamin B12 in the Spanish population: Findings from the ANIBES study

Background: Folates and vitamin B12 are key nutrients in one-carbon metabolism and related diseases. Updated and plausible information on population intakes and their major dietary sources is scarce and urgently needed in Spain in order to increase the knowledge that can lead as previous step to prevention by fortification and supplementation policies. Aims: The present study aims to evaluate main dietary folate and vitamin B12 sources and intakes in the Spanish population. Materials and methods: Results were derived from the ANIBES cross-sectional study using a nationally representative sample of the Spanish population (9–75 years, n = 2,009). Results: Food groups with the highest mean proportional contribution to total folate intakes in both males and females were vegetables (21.7–24.9%) and cereals (10.7–11.2%), while meat and meat products (26.4%) and milk and dairy products (27.3%) were for B12. Total median folate and B12 intakes amongst women were 156.3 μg/d and 4.0 μg/d while for men were 163.6 μg/d and 4.5 μg/d, respectively. In all age groups, vitamin intakes were significantly higher in plausible than in non-plausible energy reporters. Conclusion: A limited number of participants had adequate folate intakes, whereas vitamin B12 intakes were adequate for practically the entire population. There is a clear need for improving folates intake in the Spanish population.The study was financially supported by a grant from Coca-Cola Iberia through an agreement with the Spanish Nutrition Foundation (Fundación Española de la Nutrición (FEN))

Diagnostic yield of 18F-FDG PET/CT in suspected diagnosis of vascular graft infection: A prospective cohort study

Background. Prosthetic vascular graft infection (PVGI) is a severe complication associated with high morbidity and mortality. Clinical diagnosis is complex, requiring image testing such as CT angiography or leukocyte scintigraphy, which has considerable limitations. The aim of this study was to know the diagnostic yield of PET/CT with 18F-Fluorodeoxyglucose (18F-FDG) in patients with suspected PVGI. Methods. We performed a prospective cohort study including 49 patients with suspected PVGI, median age of 62 ± 14 years. Three uptake patterns were defined following published recommendations: (i) focal, (ii) patched (PVGI criteria), and (iii) diffuse (no PVGI criterion). Results. Sensitivity, specificity, and positive and negative predictive values for 18F-FDG-PET/ CT were 88%, 79%, 67%, and 93%, respectively. 18F-FDG-PET/CT identified 14/16 cases of PVGI showing a focal (n = 10) or patched pattern (n = 4), being true negative in 26/33 cases with either a diffuse pattern (n = 16) or without uptake (n = 10). Five of the seven false-positive cases (71%) showed a patched pattern and all coincided with the application of adhesives for PVG placement. Conclusions. 18F-FDG-PET/CT is a useful technique for the diagnosis of PVGI. A patched pattern on PET/CT in patients in whom adhesives were applied for prosthetic vascular graft placement does not indicate infection. (J Nucl Cardiol 2018) Key Words: Fluorodeoxyglucose (FDG) Æ diagnostic and prognostic application Æ PET/CT imagin

ESTUDIOS QUÍMICOS DE AMBROSIA CUMANENSIS KUNTH EN PANAMÁ

Ambrosia cumanesis K (Asteraceae), known as Altamisa, is an aromatic species widely distributed throughout the country. Its use is associated with the treatment of kidney, anti-inflammatory, antiparasitic and laxative disorders. The chemical characterization of the aqueous and ethanolic extracts of the leaves of A. cumanesis K. was carried out, as well as of its essential oil, in order to evaluate its biological activity. Phytochemical screening tests were carried out to identify secondary metabolites, chromatographic methods were used for the purification of plant extracts and the identification of the main compounds was carried out by IR and NMR spectroscopy, and by CG-MS for the essential oil. The chemical analysis of the ethanolic extracts of the fresh leaves of Ambrosia cumanensis Kunth has led to the identification of alkaloids, terpenes and flavonoids as the major secondary metabolites. The extraction and purification of the extracts led to the isolation of three (3) known compounds, psilostachyn A, psilostachyn C and camphor. The analysis of the chemical composition of the essential oil of this plant has led to the identification of 25 compounds and the possible correlation between the composition variability and the seasonality. The microbiological activity of the ethanolic extracts and the essential oil was evaluated by inhibition halo, showing selective antimicrobial activity for the essential oil. This is the first report of chemical studies on Ambrosia cumanensis Kunth in Panama.Ambrosia cumanesis K (Asteraceae), conocida como Altamisa, es una especie aromática distribuida ampliamente en el país. Se ha asociado su uso al tratamiento de afecciones renales, como antiinflamatorio, antiparasitario y purgante, por lo que se realizó la caracterización química de los extractos acuosos y etanólicos de las hojas de A. cumanesis K., así como de su aceite esencial, con el fin de evaluar su actividad biológica. Se llevaron a cabo pruebas de tamizaje fitoquímico para identificación de metabolitos secundarios, se emplearon métodos cromatográficos para la purificación de los extractos vegetales y la identificación de los compuestos principales se realizó por espectroscopía IR y de RMN, y por CG-MS para el caso del aceite esencial. El análisis químico de los extractos etanólicos de las hojas frescas de A. cumanensis Kunth llevó a la identificación de alcaloides, terpenos y flavonoides como los metabolitos secundarios mayoritarios. La extracción y purificación de los extractos llevó al aislamiento de tres (3) compuestos conocidos, psilostachina A, psilostachina C y alcanfor. El análisis de la composición química del aceite esencial de esta planta ha producido la identificación de 25 compuestos, y mostró que la composición puede variar dependiendo de la estacionalidad. La actividad microbiológica de los extractos etanólicos y del aceite esencial fue evaluada por halo de inhibición, mostrando actividad antimicrobiana selectiva para el aceite esencial. Este es el primer reporte de estudios químicos sobre Ambrosia cumanensis Kunth en Panamá

Percepciones de riesgo al covid-19 entre jóvenes de comunidades indígenas y rurales del sureste de México

Introduction. On January 30, 2020, the WHO declared a health emergency due to the appearance of a new coronavirus: SARS-CoV-2, which causes the COVID-19 disease. In the early stages of a pandemic, adopting precautionary behavior is crucial to preventing the rapid spread of the disease. Objective. Analyze the perceptions of risk, emotions and behaviors of young students residing in rural and indigenous regions of great marginalization of Chiapas, Mexico, on COVID-19. Methods. A survey was applied to young students of the Colegio de Bachilleres de Chiapas, located in 17 indigenous and rural municipalities with high and very high marginalization. Results. 971 young people answered the survey; 47.6% were speakers of an indigenous language. 92.2% indicated knowing or having heard about COVID-19, 88.4% believe in its existence and 76.4% indicated feeling threatened or very threatened. 39.2% believe that the virus was created in a laboratory and 26.9% believe that it was made by the government. Conclusions. It is necessary to implement health promotion and education strategies with an intercultural approach that helps to counteract misinformation and build trust in the authorities and the health response to face the pandemicIntroducción. El 30 de enero de 2020, la OMS declaró emergencia sanitaria por la aparición de un nuevo coronavirus: SARS-CoV-2, que provoca la enfermedad COVID-19. En las primeras fases de una pandemia, adoptar conductas de precaución es crucial para prevenir una rápida propagación de la enfermedad. Objetivo. Analizar las percepciones de riesgo, emociones y comportamientos de jóvenes estudiantes residentes de regiones rurales e indígenas de gran marginación de Chiapas, México, sobre el COVID-19. Metódos. Se aplicó una encuesta a jóvenes estudiantes del Colegio de Bachilleres de Chiapas, ubicados en 17 municipios indígenas y rurales de alta y muy alta marginación. Resultados. 971 jóvenes respondieron la encuesta; 47.6% fueron hablantes de alguna lengua indígena. 92.2% señaló conocer o haber escuchado sobre COVID-19, el 88.4% cree en su existencia y el 76.4% señaló sentirse amenazado o muy amenazado. El 39.2% cree que el virus fue creado en un laboratorio y el 26.9% opina que fue hecho por el gobierno. Conclusiones. Es necesario implementar estrategias de promoción y educación para la salud con enfoque intercultural que contribuya a contrarrestar la desinformación y generar confianza en las autoridades y la respuesta sanitaria para hacer frente a la pandemia

Perceptions of risk to COVID-19 among young people from indigenous and rural communities in Southeast Mexico

Introducción. El 30 de enero de 2020, la OMS declaró emergencia sanitaria por la aparición de un nuevo coronavirus: SARS-CoV-2, que provoca la enfermedad COVID-19. En las primeras fases de una pandemia, adoptar conductas de precaución es crucial para prevenir una rápida propagación de la enfermedad. Objetivo. Analizar las percepciones de riesgo, emociones y comportamientos de jóvenes estudiantes residentes de regiones rurales e indígenas de gran marginación de Chiapas, México, sobre el COVID-19. Metódos. Se aplicó una encuesta a jóvenes estudiantes del Colegio de Bachilleres de Chiapas, ubicados en 17 municipios indígenas y rurales de alta y muy alta marginación. Resultados. 971 jóvenes respondieron la encuesta; 47.6% fueron hablantes de alguna lengua indígena. 92.2% señaló conocer o haber escuchado sobre COVID-19, el 88.4% cree en su existencia y el 76.4% señaló sentirse amenazado o muy amenazado. El 39.2% cree que el virus fue creado en un laboratorio y el 26.9% opina que fue hecho por el gobierno. Conclusiones. Es necesario implementar estrategias de promoción y educación para la salud con enfoque intercultural que contribuya a contrarrestar la desinformación y generar confianza en las autoridades y la respuesta sanitaria para hacer frente a la pandemia.ABSTRACT Introduction. On January 30, 2020, the WHO declared a health emergency due to the appearance of a new coronavirus: SARS-CoV-2, which causes the COVID-19 disease. In the early stages of a pandemic, adopting precautionary behavior is crucial to preventing the rapid spread of the disease. Objective. Analyze the perceptions of risk, emotions and behaviors of young students residing in rural and indigenous regions of great marginalization of Chiapas, Mexico, on COVID-19. Methods. A survey was applied to young students of the Colegio de Bachilleres de Chiapas, located in 17 indigenous and rural municipalities with high and very high marginalization. Results. 971 young people answered the survey; 47.6% were speakers of an indigenous language. 92.2% indicated knowing or having heard about COVID-19, 88.4% believe in its existence and 76.4% indicated feeling threatened or very threatened. 39.2% believe that the virus was created in a laboratory and 26.9% believe that it was made by the government. Conclusions. It is necessary to implement health promotion and education strategies with an intercultural approach that helps to counteract misinformation and build trust in the authorities and the health response to face the pandemic

A contemporary picture of enterococcal endocarditis

BACKGROUND: Enterococcal endocarditis (EE) is a growing entity in Western countries. However, quality data from large studies is lacking. OBJECTIVES: The purpose of this study was to describe the characteristics and analyze the prognostic factors of EE in the GAMES cohort. METHODS: This was a post hoc analysis of a prospectively collected cohort of patients from 35 Spanish centers from 2008 to 2016. Characteristics and outcomes of 516 cases of EE were compared with those of 3,308 cases of nonenterococcal endocarditis (NEE). Logistic regression and Cox proportional hazards regression analysis were performed to investigate risk factors for in-hospital and 1-year mortality, as well as relapses. RESULTS: Patients with EE were significantly older; more frequently presented chronic lung disease, chronic heart failure, prior endocarditis, and degenerative valve disease; and had higher median age-adjusted Charlson score. EE more frequently involved the aortic valve and prosthesis (64.3% vs. 46.7%; p < 0.001; and 35.9% vs. 28.9%; p = 0.002, respectively) but less frequently pacemakers/defibrillators (1.5% vs. 10.5%; p < 0.001), and showed higher rates of acute heart failure (45% vs. 38.3%; p = 0.005). Cardiac surgery was less frequently performed in EE (40.7% vs. 45.9%; p = 0.024). No differences in in-hospital and 1-year mortality were found, whereas relapses were significantly higher in EE (3.5% vs. 1.7%; p = 0.035). Increasing Charlson score, LogEuroSCORE, acute heart failure, septic shock, and paravalvular complications were risk factors for mortality, whereas prior endocarditis was protective and persistent bacteremia constituted the sole risk factor for relapse. CONCLUSIONS: Besides other baseline and clinical differences, EE more frequently affects prosthetic valves and less frequently pacemakers/defibrillators. EE presents higher rates of relapse than NEE. Copyright © 2020 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved. KEYWORDS: enterococci; epidemiology; heart failure; infective endocarditis; prosthetic valves; relapse

Optimal Timing for Cardiac Surgery in Infective Endocarditis with Neurological Complications: A Narrative Review

In patients with infective endocarditis and neurological complications, the optimal timing for cardiac surgery is unclear due to the varied risk of clinical deterioration when early surgery is performed. The aim of this review is to summarize the best evidence on the optimal timing for cardiac surgery in the presence of each type of neurological complication. An English literature search was carried out from June 2018 through July 2022. The resulting selection, comprising observational studies, clinical trials, systematic reviews and society guidelines, was organized into four sections according to the four groups of neurological complications: ischemic, hemorrhagic, infectious, and asymptomatic complications. Cardiac surgery could be performed without delay in cases of ischemic vascular neurological complication (provided the absence of severe damage, which can be avoided with the performance of mechanical thrombectomy in cases of major stroke), as well as infectious or asymptomatic complications. In the presence of intracranial hemorrhage, a delay of four weeks is recommended for most cases, although recent studies have suggested that performing cardiac surgery within four weeks could be a suitable option for selected cases. The findings of this review are mostly in line with the recommendations of the current European and American infective endocarditis guidelines