Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole

Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization

Recursos y estrategias para la enseñanza de la estadística y la analítica de datos en la educación superior

The purpose of this study is to present a literature review analysis on the teaching of statistics and data analytics in higher education. The assessment includes examining the role played by specific factors as mediators of teaching-learning processes. These factors include: disciplinary and pedagogical training of teachers, teaching styles, classroom experiences, use of didactic resources, and context. The literature review revealed that there is a need to create and develop innovative learning environments that transform the teaching of statistics at the university level, starting from rethinking the contents and curricula to favor the social dimension and the humanistic and complex thinking of students. It is concluded that, currently, the generalization of digital media and social networks imposes new challenges for teaching statistics and data analytics in the context of data science, machine learning, and big data.El propósito de este estudio es presentar una reflexión documentada frente a la enseñanza de la estadística y la analítica de datos en la educación superior. Se analiza el papel que cumplen factores como la formación disciplinar y pedagógica de los docentes, sus estilos de enseñanza, las experiencias en el aula de clase, el uso de recursos didácticos y el contexto, como mediadores de los procesos de enseñanza-aprendizaje. Como resultado de la referenciación de autores realizada, se expone frente a la necesidad de crear y desarrollar ambientes de aprendizaje innovadores que transformen la enseñanza de la estadística a nivel universitario, a partir de repensar los contenidos y currículos para favorecer la dimensión socio humanística y el pensamiento complejo de los estudiantes. Se concluye que actualmente la generalización de medios digitales y las redes sociales constituyen un nuevo reto para la enseñanza de la estadística y la analítica de datos en su integración con ciencia de datos, aprendizaje automático y macrodatos

Contribution of the Residual Body in the Spatial Organization of Toxoplasma gondii Tachyzoites within the Parasitophorous Vacuole

properly cited. Toxoplasma gondii proliferates and organizes within a parasitophorous vacuole in rosettes around a residual body and is surrounded by a membranous nanotubular network whose function remains unclear. Here, we characterized structure and function of the residual body in intracellular tachyzoites of the RH strain. Our data showed the residual body as a body limited by a membrane formed during proliferation of tachyzoites probably through the secretion of components and a pinching event of the membrane at the posterior end. It contributes in the intravacuolar parasite organization by the membrane connection between the tachyzoites posterior end and the residual body membrane to give place to the rosette conformation. Radial distribution of parasites in rosettes favors an efficient exteriorization. Absence of the network and presence of atypical residual bodies in a ΔGRA2-HXGPRT knock-out mutant affected the intravacuolar organization of tachyzoites and their exteriorization

Spanish Validation of the Flourishing Scale in the General Population

Well-being research and its measurement have grown in the last two decades. The objective of this study was to adapt and validate the Flourishing Scale in a sample of Spanish adults. This was a cross-sectional study using a non-probabilistic sample of 999 Spanish general adult population participants. The psychometric properties of the scale were analysed from an exploratory and confirmatory perspective. Exploratory factor analysis showed a one-factor solution explaining 42.3% of the variance; an internal consistency of .846; temporal reliability correlation of .749; convergent validity with the Satisfaction with Life Scale of .521 and criterion validity with positive and negative affect (PANAS), pessimism and optimism (LOT-R) ranging from .270 to .488. Confirmatory factor analysis testing the one-factor solution showed a χ2 of 65.57 df = 20; CFI of .982, RMSEA of .06, average variance extracted index of .518 and composite reliability index of .841. Results showed that the Spanish version of the FS is a reliable and valid method for measuring high levels of well-bein

Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace

Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.The autonomous government of Castilla y León (Project BU268A11-2

Long-term survival in patients with non-small cell lung cancer and synchronous brain metastasis treated with whole-brain radiotherapy and thoracic chemoradiation

<p>Abstract</p> <p>Background</p> <p>Brain metastases occur in 30-50% of Non-small cell lung cancer (NSCLC) patients and confer a worse prognosis and quality of life. These patients are usually treated with Whole-brain radiotherapy (WBRT) followed by systemic therapy. Few studies have evaluated the role of chemoradiotherapy to the primary tumor after WBRT as definitive treatment in the management of these patients.</p> <p>Methods</p> <p>We reviewed the outcome of 30 patients with primary NSCLC and brain metastasis at diagnosis without evidence of other metastatic sites. Patients were treated with WBRT and after induction chemotherapy with paclitaxel and cisplatin for two cycles. In the absence of progression, concurrent chemoradiotherapy for the primary tumor with weekly paclitaxel and carboplatin was indicated, with a total effective dose of 60 Gy. If disease progression was ruled out, four chemotherapy cycles followed.</p> <p>Results</p> <p>Median Progression-free survival (PFS) and Overall survival (OS) were 8.43 ± 1.5 and 31.8 ± 15.8 months, respectively. PFS was 39.5% at 1 year and 24.7% at 2 years. The 1- and 2-year OS rates were 71.1 and 60.2%, respectively. Three-year OS was significantly superior for patients with N0-N1 stage disease vs. N2-N3 (60 vs. 24%, respectively; Response rate [RR], 0.03; <it>p</it>= 0.038).</p> <p>Conclusions</p> <p>Patients with NSCLC and brain metastasis might benefit from treatment with WBRT and concurrent thoracic chemoradiotherapy. The subgroup of N0-N1 patients appears to achieve the greatest benefit. The result of this study warrants a prospective trial to confirm the benefit of this treatment.</p

Odorous and pungent attributes of mixed and unmixed odorants

In order to explore functional properties of the olfactory and common chemical senses as well as their relation to the total nasal sensation experienced, various concentrations of two pungent odorants were presented alone and in the presence of different backgrounds of the other irritant. Stimuli comprised formaldehyde (at 1.0, 3.5, 6.9, and 16.7 ppm), ammonia (at 210, 776, 1,172, and 1,716 ppm), and their 16 possible binary mixtures. Subjects were asked to estimate the total nasal perceived intensity, and then to assess the olfactory (odor) and common chemical (pungency) attributes of the evoked sensations. The results showed that stimulus-response functions for pungency are steeper than those for odor. Furthermore, odor was always hypoadditive in mixtures (i.e., mixtures were perceived as less intense than the sum of their components), whereas pungency was, mainly, additive, and even suggested hyperadditivity. Total perceived intensity of the stimuli, alone and in mixtures, followed the stimulus-response patterns for pungency, which, therefore, emerged as the dominating attribute used by subjects in scaling the explored range of concentrations. The relationship between total nasal perceived intensity of the mixtures and that of their components reflected hypoaddition, resembling the outcome for the odor attribute