Comparison of Otolith and Scale Age Determinations for Freshwater Drum from the Mississippi River

A comparative aging study was conducted using scales and otoliths from 123 freshwater drum collected in Pool 14 of the Mississippi River. Two independent readings by 2 investigators resulted in full agreement on ages assigned using otoliths, but only 64% agreement using the scale method. A final age was determined for the remaining scale samples based on the most commonly assigned age. However, there was no agreement on assigned ages for 12% of the scales examined. Otoliths were validated as an accurate method for aging freshwater drum by age frequency histograms for 3 consecutive years. The marked 3-year periodicity in appearance of strong year-classes allowed these strong year-classes to be followed through successive years of study providing a check on the reliability of this aging method. Scales were concluded to be only 61% reliable for aging freshwater drum. The observed trend indicated that assigned ages using scales were commonly overestimated for fish age 9 and younger and underestimated for older fish

Relative Survival of Three Sizes of Walleyes Stocked into Illinois Lakes

The ability to differentially batch-mark several size-groups of fish stocked concurrently in lakes or rivers makes it possible to compare survival within a single year. We evaluated stocking of walleyes Stizostedion vitreum in Illinois during 1991–1996. Ten lakes were stocked with combinations of two or three sizes of walleyes, including fry and small (50-mm) and large (100-mm) fingerlings. This permitted a total of 73 lake-year comparisons: fry versus large fingerlings (23 lake-years), fry versus small fingerlings (29 lake-years), and small versus large fingerlings (21 lake-years). Stocked fish were differentially marked with oxytetracycline or fin clips. Electrofishing catch per effort, relative survival, and population estimates were used in conjunction with production costs to compare size-based contributions and survival. Survival generally favored fingerlings over fry (70% of lake-years for small fingerlings, 67% for large fingerlings) and small fingerlings over large fingerlings (72% of lake-years). In some lake-years, fry and large fingerlings had the highest survival. Fish stocked as fry and small fingerlings were larger than large fingerlings beginning their third year of growth. Based on our results, stocking small fingerlings will generally be more cost-effective than stocking fry or large fingerlings. However, if fry or large fingerlings are available, stocking assessment on specific lakes may allow biologists to determine particular lakes where alternative sizes could be economically feasible

Gear-Specific Population Demographics of Channel Catfish in a Large Midwestern River

Various gear types have been used to sample populations of channel catfish Ictalurus punctatus in lotic systems. However, these gears produce different population characteristics (i.e., recruitment, growth, and mortality). We compared the population demographics of channel catfish in the Wabash River, Indiana, sampled with baited 25- and 32-mm-bar mesh hoop nets and three-phase alternating current (AC) electrofishing. Based on catch per unit effort, the relative abundance of channel catfish sampled with 32-mm hoop nets was lower than that of fish sampled with 25-mm hoop nets and AC electrofishing. Each gear type also resulted in a different length frequency, mean length increasing progressively in sampling with 25-mm hoop nets, 32-mm hoop nets, and AC electrofishing. Similarly, age-frequency distributions differed among gears. The 25-mm hoop nets biased the age structure toward younger individuals (mean age = 2.5), whereas both 32-mm hoop nets (mean age = 4.0) and AC electrofishing (mean age = 5.8) included older fish. Catch-curve analysis generated different mortality rates for the three gear types, the mortality rate being highest (50%) in fish sampled with 25-mm hoop nets. Gear-specific size and age structures led to differences in von Bertalanffy statistics among the 25-mm hoop nets and AC electrofishing, while the results for 32-mm hoop nets were uninterpretable. Because the different gears led to conflicting parameter estimates, management practices based on sampling with single gears may be contradictory. Given the differences in gear selectivity, biologists need to approach management cautiously until calibration to the true size and age structure is conducted

Operational Comparison of Three Electrofishing Systems

Three different electrofishing systems were compared to determine their relative efficiency with respect to species and numbers of fish collected. These results indicated that modifications or changes in electrofishing gear during a monitoring program should not be made unless it can be demonstrated that collecting efficiency is not altered

Control of a SiC 2.5 MHz resonant full-bridge inverter for inductively driven plasma

The electronic ballast of an inductively driven plasma faces a mostly inductive, variable load impedance. The SiC full-bridge inverter uses the inductive behavior to achieve zero-voltage-switching and a switching frequency of 2.5 MHz, at 3.9 kVA and 764W in the plasma. The control is realized on a modern, small TI Piccolo microcontroller with a high-resolution PWM module and compensates for the variable load impedance. We present design and experimental results of a 2.5 MHz inverter for inductively driven plasma without the need for a large FPGA controller

Spatial random downscaling of rainfall signals in Andean heterogeneous terrain.

Remotely sensed data are often used as proxies for indirect precipitation measures over data-scarce and complex-terrain areas such as the Peruvian Andes. Although this information might be appropriate for some research requirements, the extent at which local sites could be related to such information is very limited because of the resolution of the available satellite data. Downscaling techniques are used to bridge the gap between what climate modelers (global and regional) are able to provide and what decision-makers require (local). Precipitation downscaling improves the poor local representation of satellite data and helps end-users acquire more accurate estimates of water availability. Thus, a multifractal downscaling technique complemented by a heterogeneity filter was applied to TRMM (Tropical Rainfall Measuring Mission) 3B42 gridded data (spatial resolution ~ 28 km) from the Peruvian Andean high plateau or Altiplano to generate downscaled rainfall fields that are relevant at an agricultural scale (spatial resolution ~ 1 km)

Retrieving Aerosol in a Cloudy Environment: Aerosol Availability as a Function of Spatial and Temporal Resolution

The challenge of using satellite observations to retrieve aerosol properties in a cloudy environment is to prevent contamination of the aerosol signal from clouds, while maintaining sufficient aerosol product yield to satisfy specific applications. We investigate aerosol retrieval availability at different instrument pixel resolutions, using the standard MODIS aerosol cloud mask applied to MODIS data and a new GOES-R cloud mask applied to GOES data for a domain covering North America and surrounding oceans. Aerosol availability is not the same as the cloud free fraction and takes into account the technqiues used in the MODIS algorithm to avoid clouds, reduce noise and maintain sufficient numbers of aerosol retrievals. The inherent spatial resolution of each instrument, 0.5x0.5 km for MODIS and 1x1 km for GOES, is systematically degraded to 1x1 km, 2x2 km, 4x4 km and 8x8 km resolutions and then analyzed as to how that degradation would affect the availability of an aerosol retrieval, assuming an aerosol product resolution at 8x8 km. The results show that as pixel size increases, availability decreases until at 8x8 km 70% to 85% of the retrievals available at 0.5 km have been lost. The diurnal pattern of aerosol retrieval availability examined for one day in the summer suggests that coarse resolution sensors (i.e., 4x4 km or 8x8 km) may be able to retrieve aerosol early in the morning that would otherwise be missed at the time of current polar orbiting satellites, but not the diurnal aerosol properties due to cloud cover developed during the day. In contrast finer resolution sensors (i.e., 1x1 km or 2x2 km) have much better opportunity to retrieve aerosols in the partly cloudy scenes and better chance of returning the diurnal aerosol properties. Large differences in the results of the two cloud masks designed for MODIS aerosol and GOES cloud products strongly reinforce that cloud masks must be developed with specific purposes in mind and that a generic cloud mask applied to an independent aerosol retrieval will likely fail

Transition from initiation to promoter proximal pausing requires the CTD of RNA polymerase II

The C-terminal domain (CTD) of mammalian RNA polymerase II consists of 52 repeats of the consensus hepta-peptide YSPTSPS, and links transcription to the processing of pre-mRNA. Although Pol II with a CTD shortened to five repeats (Pol II Δ5) is transcriptionally inactive on chromatin templates, it is not clear whether CTD is required for promoter recognition in vivo. Here, we demonstrate that in the context of chromatin, Pol II Δ5 can bind to the c-myc promoter with the same efficiency as wild type Pol II. However, Pol II Δ5 does not form a stable initiation complex, and does not transcribe promoter proximal sequences. Fluorescence recovery after photobleaching (FRAP) experiments with cells expressing enhanced green fluorescent protein (EGFP)-tagged Δ5 or wildtype Pol II revealed a single, highly mobile Pol II Δ5 fraction whereas wildtype Pol II yielded less mobile fractions. These data suggest that CTD is not required for promoter recognition, but rather for subsequent formation of a stable initiation complex and isomerization to an elongation competent complex