Bibliography of reversed-phase partition chromatography

BIBLIOGRAPHY OF REVERSED-PHASE PARTITION CHROMATOGRAPH

Toughening of BIS maleimide resins: Synthesis and characterization of maleimide terminated poly(arylene ether) oligomers and polymers

Amine functional poly(arylene ether) sulfones were previously reported. Herein, the chemistry was extended to amorphous poly(arylene ether) ketones because of their higher fracture toughness values, relative to the polysulfones. It was demonstrated that the amino functional oligomers undergo a self-crosslinking reaction at temperatures above about 220 C. This produces an insoluble, but ductile network that has excellent resistance. A ketamine structure hypothesis was proposed and verified using solid state magic angle NMR. In most cases, the water generated upon ketamine formation is too low to produce porosity and solid networks are obtained. The stability of the ketamine networks towards hydrolysis is excellent. The chemistry was further demonstrated to be able to crosslink preformed nonfunctional poly(arylene ether) ketones if a difunctional amine was utilized. This concept has the possibility of greatly improving the creep resistance of thermoplastics. Also, a new technique was developed for converting the amine functional oligomers cleanly into maleimide structures. This method involves reacting maleic anhydride with monomeric aminophenols in the presence of solvent mixtures

Polyaryl ethers and related polysiloxane copolymer molecular coatings preparation and radiation degrdation

Poly(arylene ether sulfones) comprise a class of materials known as engineering thermoplastics which have a variety of important applications. These polymers are tough, rigid materials with good mechanical properties over a wide temperature range, and they are processed by conventional methods into products typically having excellent hydrolytic, thermal, oxidative and dimensional stability. Wholly aromatic random copolymers of hydroquinone and biphenol with 4.4 prime dichlorodiphenyl sulfone were synthesized via mechanical nucleophilic displacement. Their structures were characterized and mechanical behavior studied. These tough, ductile copolymers show excellent radiation resistance to electron beam treatment and retain much of the mechanical properties up to at least 700 Mrads under argon

Optical Variability of Narrow-Line Seyfert 1 Galaxies

We present results of a broad-band photometric study of the optical variability of six Narrow-Line Seyfert 1 (NLS1) galaxies observed at 172 epochs. We searched for microvariability on 33 nights. Strong evidence for microvariability was found only for our lowest luminosity object, NGC 4051, on one night. Weaker evidence suggests such variability on a few other nights for two other objects, but the data are not as convincing. Intra-night variability in NLS1s is thus concluded to be rare and of low amplitude. We give illustrations of how variable image quality can produce spurious variability. We find that for well-studied non-NLS1s there is a spread in the amplitude of seasonal variability (i.e., in some years an AGN is more variable than in others). We find that the means of the variability amplitudes of non-NLS1s over several seasons vary from object to object (i.e., some AGNs are, on average, more variable than others). NLS1s also show a spread in seasonal variabilities. The best-studied NLS1, Ark 564, shows a range of amplitudes of variability from season to season that is comparable to the range found in BLS1s, and in one season Ark 564 was as variable as the most variable non-NLS1. The seasonal amplitudes of variability for NLS1s are mostly in the lower half of the range of non-BLS1 seasonal amplitudes, but the absence of a suitable control sample makes a precise comparison difficult. However, on long timescales (weeks to years), NLS1s as a class are not more variable than non-NLS1s. The extreme variability seen in the X-rays was not seen in the optical. This has consequences for the models of AGNs in general as well as NLS1s in particular.Comment: Astrophysical Journal in press (tentatively scheduled for Vol. 608, June 2004 issue);47 pages; 19 figures. Electronic tables not included (contact first author if needed

Reverberation Mapping Measurements of Black Hole Masses in Six Local Seyfert Galaxies

We present the final results from a high sampling rate, multi-month, spectrophotometric reverberation mapping campaign undertaken to obtain either new or improved Hbeta reverberation lag measurements for several relatively low-luminosity AGNs. We have reliably measured thetime delay between variations in the continuum and Hbeta emission line in six local Seyfert 1 galaxies. These measurements are used to calculate the mass of the supermassive black hole at the center of each of these AGNs. We place our results in context to the most current calibration of the broad-line region (BLR) R-L relationship, where our results remove outliers and reduce the scatter at the low-luminosity end of this relationship. We also present velocity-resolved Hbeta time delay measurements for our complete sample, though the clearest velocity-resolved kinematic signatures have already been published.Comment: 52 pages (AASTeX: 29 pages of text, 8 tables, 7 figures), accepted for publication in the Astrophysical Journa

X-Ray/Ultraviolet Observing Campaign of the Markarian 279 Active Galactic Nucleus Outflow: a close look at the absorbing/emitting gas with Chandra-LETGS

We present a Chandra-LETGS observation of the Seyfert 1 galaxy Mrk 279. This observation was carried out simultaneously with HST-STIS and FUSE, in the context of a multiwavelength study of this source. The Chandra pointings were spread over ten days for a total exposure time of ~360 ks. The spectrum of Mrk279 shows evidence of broad emission features, especially at the wavelength of the OVII triplet. We quantitatively explore the possibility that this emission is produced in the broad line region (BLR). We modeled the broad UV emission lines seen in the FUSE and HST-STIS spectra following the ``locally optimally emitting cloud" approach. We find that the X-ray lines luminosity derived from the best fit BLR model can match the X-ray features, suggesting that the gas producing the UV lines is sufficient to account also for the X-ray emission. The spectrum is absorbed by ionized gas whose total column density is ~5x10^{20} cm^{-2}. The absorption spectrum can be modeled by two distinct gas components (log xi ~ 0.47 and 2.49, respectively) both showing a significant outflow velocity. However, the data allow also the presence of intermediate ionization components. The distribution of the column densities of such extra components as a function of the ionization parameter is not consistent with a continuous, power law-like, absorber, suggesting a complex structure for the gas outflow for Mrk 279 (abridged).Comment: 16 pages, 12 figures. To appear in A&

Diabetes reversal by inhibition of the low-molecular-weight tyrosine phosphatase.

Obesity-associated insulin resistance plays a central role in type 2 diabetes. As such, tyrosine phosphatases that dephosphorylate the insulin receptor (IR) are potential therapeutic targets. The low-molecular-weight protein tyrosine phosphatase (LMPTP) is a proposed IR phosphatase, yet its role in insulin signaling in vivo has not been defined. Here we show that global and liver-specific LMPTP deletion protects mice from high-fat diet-induced diabetes without affecting body weight. To examine the role of the catalytic activity of LMPTP, we developed a small-molecule inhibitor with a novel uncompetitive mechanism, a unique binding site at the opening of the catalytic pocket, and an exquisite selectivity over other phosphatases. This inhibitor is orally bioavailable, and it increases liver IR phosphorylation in vivo and reverses high-fat diet-induced diabetes. Our findings suggest that LMPTP is a key promoter of insulin resistance and that LMPTP inhibitors would be beneficial for treating type 2 diabetes

Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice.

Oxidized phospholipids (OxPL) are ubiquitous, are formed in many inflammatory tissues, including atherosclerotic lesions, and frequently mediate proinflammatory changes 1 . Because OxPL are mostly the products of non-enzymatic lipid peroxidation, mechanisms to specifically neutralize them are unavailable and their roles in vivo are largely unknown. We previously cloned the IgM natural antibody E06, which binds to the phosphocholine headgroup of OxPL, and blocks the uptake of oxidized low-density lipoprotein (OxLDL) by macrophages and inhibits the proinflammatory properties of OxPL2-4. Here, to determine the role of OxPL in vivo in the context of atherogenesis, we generated transgenic mice in the Ldlr-/- background that expressed a single-chain variable fragment of E06 (E06-scFv) using the Apoe promoter. E06-scFv was secreted into the plasma from the liver and macrophages, and achieved sufficient plasma levels to inhibit in vivo macrophage uptake of OxLDL and to prevent OxPL-induced inflammatory signalling. Compared to Ldlr-/- mice, Ldlr -/- E06-scFv mice had 57-28% less atherosclerosis after 4, 7 and even 12 months of 1% high-cholesterol diet. Echocardiographic and histologic evaluation of the aortic valves demonstrated that E06-scFv ameliorated the development of aortic valve gradients and decreased aortic valve calcification. Both cholesterol accumulation and in vivo uptake of OxLDL were decreased in peritoneal macrophages, and both peritoneal and aortic macrophages had a decreased inflammatory phenotype. Serum amyloid A was decreased by 32%, indicating decreased systemic inflammation, and hepatic steatosis and inflammation were also decreased. Finally, the E06-scFv prolonged life as measured over 15 months. Because the E06-scFv lacks the functional effects of an intact antibody other than the ability to bind OxPL and inhibit OxLDL uptake in macrophages, these data support a major proatherogenic role of OxLDL and demonstrate that OxPL are proinflammatory and proatherogenic, which E06 counteracts in vivo. These studies suggest that therapies inactivating OxPL may be beneficial for reducing generalized inflammation, including the progression of atherosclerosis, aortic stenosis and hepatic steatosis

Human Lipoxygenase Pathway Gene Variation and Association with Markers of Subclinical Atherosclerosis in the Diabetes Heart Study

Aims. Genes of the 5-lipoxygenase pathway are compelling candidates for atherosclerosis. We hypothesize that polymorphisms in ALOX12, ALOX15, ALOX5, and ALOX5AP genes are associated with subclinical atherosclerosis in multiple vascular beds. Methods. Families with two or more siblings with type 2 diabetes and their nondiabetic siblings were studied as part of the Diabetes Heart Study (DHS). European American diabetic (n = 828) and nondiabetic (n = 170) siblings were genotyped for SNPs in the ALOX12, ALOX15, ALOX5, and ALOX5AP genes. Subclinical measures of atherosclerosis (IMT, coronary (CorCP), carotid (CarCP) and aortic (AorCP) calcified plaque) were obtained. Results. Associations were observed between ALOX12 with CorCP, ALOX5 with CorCP, AorCP, and IMT, and ALOX5AP with CorCP and CarCP, independent of known epidemiologic risk factors. Further, lipoxygenase pathway SNPs that were associated with measures of atherosclerosis were associated with markers of inflammation (CRP, ICAM-1) and calcification (MGP). Conclusions. Polymorphisms within ALOX12, ALOX5, and ALOX5AP are genetically associated with subclinical atherosclerosis and with biomarkers of disease in families with type 2 diabetes. These results suggest that variants in lipoxygenase pathway genes may have pleiotropic effects on multiple components that determine risk of cardiovascular disease

A Revised Broad-Line Region Radius and Black Hole Mass for the Narrow-Line Seyfert 1 NGC 4051

We present the first results from a high sampling rate, multi-month reverberation mapping campaign undertaken primarily at MDM Observatory with supporting observations from telescopes around the world. The primary goal of this campaign was to obtain either new or improved Hbeta reverberation lag measurements for several relatively low luminosity AGNs. We feature results for NGC 4051 here because, until now, this object has been a significant outlier from AGN scaling relationships, e.g., it was previously a ~2-3sigma outlier on the relationship between the broad-line region (BLR) radius and the optical continuum luminosity - the R_BLR-L relationship. Our new measurements of the lag time between variations in the continuum and Hbeta emission line made from spectroscopic monitoring of NGC 4051 lead to a measured BLR radius of R_BLR = 1.87 (+0.54 -0.50) light days and black hole mass of M_BH = 1.73 (+0.55 -0.52) x 10^6 M_sun. This radius is consistent with that expected from the R_BLR-L relationship, based on the present luminosity of NGC 4051 and the most current calibration of the relation by Bentz et al. (2009a). We also present a preliminary look at velocity-resolved Hbeta light curves and time delay measurements, although we are unable to reconstruct an unambiguous velocity-resolved reverberation signal.Comment: 38 pages, 7 figures, accepted for publication in ApJ, changes from v1 reflect suggestions from anonymous refere