On the micro mechanics of one-dimensional normal compression

Discrete-element modelling has been used to investigate the micro mechanics of one-dimensional compression. One-dimensional compression is modelled in three dimensions using an oedometer and a large number of particles, and without the use of agglomerates. The fracture of a particle is governed by the octahedral shear stress within the particle due to the multiple contacts and a Weibull distribution of strengths. Different fracture mechanisms are considered, and the influence of the distribution of fragments produced for each fracture on the global particle size distribution and the slope of the normal compression line is investigated. Using the discrete-element method, compression is related to the evolution of a fractal distribution of particles. The compression index is found to be solely a function of the strengths of the particles as a function of size

The unique chemistry of Eastern Mediterranean water masses selects for distinct microbial communities by depth

Peer reviewedPublisher PD

Graduate dress code: How undergraduates are planning to use hair, clothes and make-up to smooth their transition to the workplace

This article explores the relationship between students’ identities, their ideas about professional appearance and their anticipated transition to the world of work. It is based on a series of semi-structured interviews with 13 students from a vocationally-focused university in England. It was found that participants viewed clothing and appearance as an important aspect of their transition to the workplace. They believed that, if carefully handled, their appearance could help them to fit in and satisfy the expectations of employers, although some participants anticipated that this process of fitting in might compromise their identity and values. The article addresses students’ anticipated means of handling the tension between adapting to a new environment and ‘being themselves’. It is argued that the way this process is handled is intertwined with wider facets of identity – most notably those associated with gender.The article is based on research funded by the University of Derby. © 2015 IP Publishing Ltd. ((http://www.ippublishing.com). Reproduced by permission

Draft genome sequences of five recent human uropathogenic Escherichia coli isolates

This study reports the release of draft genome sequences of five isolates of uropathogenic Escherichia coli (UPEC), isolated from patients suffering from uncomplicated cystitis in 2012 in Ann Arbor, Michigan. Phylogenetic analyses revealed that these strains belonged to E. coli phylogroups B2 and D and are closely related to known UPEC strains. Comparative genomic analysis revealed that more conserved proteins were shared between these recent isolates and UPEC strains causing cystitis than those causing pyelonephritis. Additional genomic comparisons identified that three isolates encode a type III secretion system (T3SS) and a putative T3SS effector gene cluster along with an invasin‐like outer membrane protein. The presence of T3SS genes is a rare occurrence among UPEC strains. These genomes further substantiate the heterogeneity of the gene pool of UPEC and provide a foundation for comparative genomic studies using recent clinical isolates.This publication briefly describes the draft genomes of five recent human uropathogenic (UPEC) Escherichia coli isolates. UPEC are of increasing importance to human health. The genomes of these new isolates are clearly and simply described and will be of great utility and interest to this research community.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136326/1/fim12059.pd

Siklus Hidup dan Statistik Demografi Kutukebul Bemisia Tabaci (Gennadius) (Hemiptera: Aleyrodidae) Biotipe B dan Non-B pada Tanaman Cabai (Capsicum Annuum L.)

Kutukebul Bemisia tabaci (Gennadius) biotipe B yang juga dikenal sebagai Bemisia argentifolii (Gennadius) merupakan jenis biotipe yang lebih ganas dalam merusak tanaman dibandingkan dengan biotipe non-B. Saat ini kutukebul B. tabaci biotipe B telah dilaporkan keberadaanya di Indonesia. Informasi dasar, seperti siklus hidup, lama hidup, keperidian, dan kemampuan berkembang biak kutukebul sangat diperlukan sebagai dasar dalam menyusun strategi pengendalian hama kutukebul tersebut. Penelitian bertujuan untuk mempelajari siklus hidup dan statistik demografi kutukebul B. tabaci biotipe B dan non-B pada tanaman cabai. Penelitian dilakukan dengan mengamati perkembangan sejak telur sampai menjadi dewasa di grow chamber yang terkontrol suhu dan pencahayaannya. Pengamatan dilakukan terhadap beberapa aspek biologi dan beberapa parameter statistik demografi. Hasil penelitian menunjukkan bahwa kutukebul B. tabaci biotipe B memiliki beberapa aspek biologi yang berbeda dengan non-B pada tanaman cabai. Siklus hidup kutukebul B. tabaci biotipe B dan non-B masing-masing adalah 33,27 dan 30,86 hari. Kutukebul B. tabaci biotipe B memiliki laju reproduksi bersih (R0) yang hampir sama dengan biotipe non-B, begitu juga rataan masa generasinya. Namun demikian, laju pertambahan intrinsik (r) kutukebul B. tabaci biotipe B 2,5 kali lebih cepat dibandingkan biotipe non-B. Kutukebul B. tabaci biotipe B berlipat ganda populasinya (DT) 2 kali lebih cepat dibandingkan biotipe non-B. Kutukebul B. tabaci biotipe B berpotensi lebih berbahaya dibandingkan dengan biotipe non-B sehingga perlu diwaspadai keberadaannya

EXAFS study of lead-free relaxor ferroelectric BaTi(1-x)Zr(x)O3 at the Zr K-edge

Extended X-ray absorption fine structure (EXAFS) experiments at the Zr K-edge were carried out on perovskite relaxor ferroelectrics BaTi(1-x)Zr(x)O3 (BTZ) (x = 0.25, 0.30, 0.35), and on BaZrO3 for comparison. Structural information up to 4.5 A around the Zr atoms is obtained, revealing that the local structure differs notably from the average Pm-3m cubic structure deduced from X-ray diffraction. In particular, our results show that the distance between Zr atoms and their first oxygen neighbors is independent of the Zr substitution rate x and equal to that measured in BaZrO3, while the X-ray cubic cell parameter increases linearly with x. Furthermore, we show that the Zr atoms tend to segregate in Zr-rich regions. We propose that the relaxor behavior in BTZ is linked to random elastic fields generated by this particular chemical arrangement, rather than to random electric fields as is the case in most relaxors.Comment: 13 pages, 12 figures, 4 tables. Submitted to Phys. Rev.

Complete homochirality induced by the nonlinear autocatalysis and recycling

A nonlinear autocatalysis of a chiral substance is shown to achieve homochirality in a closed system, if the back-reaction is included. Asymmetry in the concentration of two enantiomers or the enantiometric excess increases due to the nonlinear autocatalysis. Furthermore, when the back-reaction is taken into account, the reactant supplied by the decomposition of the enantiomers is recycled to produce more and more the dominant one, and eventually the homochirality is established.Comment: 4 pages, 2 figure

Microbial Response to the MC-252 Oil and Corexit 9500 in the Gulf of Mexico

The Deepwater Horizon spill released over 4.1 million barrels of crude oil into the Gulf of Mexico. In an effort to mitigate large oil slicks, the dispersant Corexit 9500 was sprayed onto surface slicks and injected directly at the wellhead at water depth of 1,500 m. Several research groups were involved in investigating the fate of the MC-252 oil using newly advanced molecular tools to elucidate microbial interactions with oil, gases, and dispersant. Microbial community analysis by different research groups revealed that hydrocarbon degrading bacteria belonging to Oceanospirillales, Colwellia, Cycloclasticus, Rhodobacterales, Pseudoalteromonas, and methylotrophs were found enriched in the contaminated water column. Presented here is a comprehensive overview of the ecogenomics of microbial degradation of MC-252 oil and gases in the water column and shorelines. We also present some insight into the fate of the dispersant Corexit 9500 that was added to aid in oil dispersion process. Our results show the dispersant was not toxic to the indigenous microbes at concentrations added, and different bacterial species isolated in the aftermath of the spill were able to degrade the various components of Corexit 9500 that included hydrocarbons, glycols, and dioctyl sulfosuccinate

Identification of Critical Paraoxonase 1 Residues Involved in High Density Lipoprotein Interaction

Paraoxonase 1 (PON1) is a high density lipoprotein (HDL)-associated protein with atherosclerosis-protective and systemic anti-oxidant functions. We recently showed that PON1, myeloperoxidase, and HDL bind to one another in vivo forming a functional ternary complex (Huang, Y., Wu, Z., Riwanto, M., Gao, S., Levison, B. S., Gu, X., Fu, X., Wagner, M. A., Besler, C., Gerstenecker, G., Zhang, R., Li, X. M., Didonato, A. J., Gogonea, V., Tang, W. H., et al. (2013) J. Clin. Invest. 123, 3815–3828). However, specific residues on PON1 involved in the HDL-PON1 interaction remain unclear. Unambiguous identification of protein residues involved in docking interactions to lipid surfaces poses considerable methodological challenges. Here we describe a new strategy that uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when incorporated into HDL, was used to identify amino acid residues on PON1 that directly interact with the lipoprotein phospholipid surface. Several specific PON1 residues (Leu-9, Tyr-185, and Tyr-293) were identified through covalent cross-links with the lipid probes using affinity isolation coupled to liquid chromatography with on-line tandem mass spectrometry. Based upon the crystal structure for PON1, the identified residues are all localized in relatively close proximity on the surface of PON1, defining a domain that binds to the HDL lipid surface. Site-specific mutagenesis of the identified PON1 residues (Leu-9, Tyr-185, and Tyr-293), coupled with functional studies, reveals their importance in PON1 binding to HDL and both PON1 catalytic activity and stability. Specifically, the residues identified on PON1 provide important structural insights into the PON1-HDL interaction. More generally, the new photoactivatable and affinity-tagged lipid probe developed herein should prove to be a valuable tool for identifying contact sites supporting protein interactions with lipid interfaces such as found on cell membranes or lipoproteins