Intracellular Penetration and Accumulation of Radiographic Contrast Media in the Rat Kidney

Radiographic iodine-containing contrast media (meglumine calcium metrizoate, iohexol and meglumine sodium ioxaglate) were injected intravenously in rats. At various intervals after exposure, in situ cryofixation of kidneys was performed. Thin, freeze-dried cryosections were examined by electron microscopy and X-ray microanalysis. In endothelial cells, erythrocytes and tubular cells high dry weight concentrations of iodine were found. Twenty-four hours after iohexol was injected, no trace of iodine was found in the plasma, microvilli or the nuclei of the tubular cells. Small organelle-like compartments in the cytoplasm of the proximal tubular cells contained high concentrations of iodine, whereas no iodine was found in the surrounding cytoplasm. Since no metabolism of contrast medium has been demonstrated, the iodine signals must be emitted from contrast medium molecules. Other elements were also measured, with the concentrations being always within the ranges found in tubular cells of control animals. The detection of intracellular contrast thus does not seem to be an artifact due to cell injury, but rather represents a physiological event in healthy cells in the rat kidney. Our results are in contradiction to the prevailing opinion that contrast media do not enter healthy cells. However, previous conclusions have been based on the use of conventional preparation methods, and the highly water soluble contrast molecules may have been lost during the different steps of fixation and processing

Inertial range scaling in numerical turbulence with hyperviscosity

Numerical turbulence with hyperviscosity is studied and compared with direct simulations using ordinary viscosity and data from wind tunnel experiments. It is shown that the inertial range scaling is similar in all three cases. Furthermore, the bottleneck effect is approximately equally broad (about one order of magnitude) in these cases and only its height is increased in the hyperviscous case--presumably as a consequence of the steeper decent of the spectrum in the hyperviscous subrange. The mean normalized dissipation rate is found to be in agreement with both wind tunnel experiments and direct simulations. The structure function exponents agree with the She-Leveque model. Decaying turbulence with hyperviscosity still gives the usual t^{-1.25} decay law for the kinetic energy, and also the bottleneck effect is still present and about equally strong.Comment: Final version (7 pages

Loss of heterozygosity is related to p53 mutations and smoking in lung cancer

Carcinogenesis results from an accumulation of several genetic alterations. Mutations in the p53 gene are frequent and occur at an early stage of lung carcinogenesis. Loss of multiple chromosomal regions is another genetic alteration frequently found in lung tumours. We have examined the association between p53 mutations, loss of heterozygosity (LOH) at frequently deleted loci in lung cancer, and tobacco exposure in 165 tumours from non-small cell lung cancer (NSCLC) patients. A highly significant association between p53 mutations and deletions on 3p, 5q, 9p, 11p and 17p was found. There was also a significant correlation between deletions at these loci. 86% of the tumours with concordant deletion in the 4 most involved loci (3p21, 5q11–13, 9p21 and 17p13) had p53 mutations as compared to only 8% of the tumours without deletions at the corresponding loci (P< 0.0001). Data were also examined in relation to smoking status of the patients and histology of the tumours. The frequency of deletions was significantly higher among smokers as compared to non-smokers. This difference was significant for the 3p21.3 (hMLH1 locus), 3p14.2 (FHIT locus), 5q11–13 (hMSH3 locus) and 9p21 (D9S157 locus). Tumours with deletions at the hMLH1 locus had higher levels of hydrophobic DNA adducts. Deletions were more common in squamous cell carcinomas than in adenocarcinomas. Covariate analysis revealed that histological type and p53 mutations were significant and independent parameters for predicting LOH status at several loci. In the pathogenesis of NSCLC exposure to tobacco carcinogens in addition to clonal selection may be the driving force in these alterations. © 2001 Cancer Research Campaign http://www.bjcancer.co

The scaling properties of dissipation in incompressible isotropic three-dimensional magnetohydrodynamic turbulence

The statistical properties of the dissipation process constrain the analysis of large scale numerical simulations of three dimensional incompressible magnetohydrodynamic (MHD) turbulence, such as those of Biskamp and Muller [Phys. Plasmas 7, 4889 (2000)]. The structure functions of the turbulent flow are expected to display statistical self-similarity, but the relatively low Reynolds numbers attainable by direct numerical simulation, combined with the finite size of the system, make this difficult to measure directly. However, it is known that extended self-similarity, which constrains the ratio of scaling exponents of structure functions of different orders, is well satisfied. This implies the extension of physical scaling arguments beyond the inertial range into the dissipation range. The present work focuses on the scaling properties of the dissipation process itself. This provides an important consistency check in that we find that the ratio of dissipation structure function exponents is that predicted by the She and Leveque [Phys. Rev. Lett 72, 336 (1994)] theory proposed by Biskamp and Muller. This supplies further evidence that the cascade mechanism in three dimensional MHD turbulence is non-linear random eddy scrambling, with the level of intermittency determined by dissipation through the formation of current sheets.Comment: 9 pages, 6 figures. Figures embedded in text. Typos corrected in text and references. Published in Physics of Plasmas. Abstract can be found at:http://link.aip.org/link/?php/12/02230

Naturally Occurring Agglutinins for Pheasant Red Blood Cells

Sera from 24 vertebrate species and 3 antisera specific for human type A, B and Rh-D cells, respectively, were tested for their ability to agglutinate red blood cells of ring-necked pheasants (Phasianus sp.). Normal sera were grouped on the basis of agglutination titer, and the majority were included in the moderate and strong groups. Differential agglutination was limited, however. Substantial variation in ability to agglutinate pheasant cells occurred among sera of individuals of the same species. The usefulness of natural sera tested appears limited in the investigation of blood group factors of ring-necked pheasants

Revisiting Semantics of Interactions for Trace Validity Analysis

Interaction languages such as MSC are often associated with formal semantics by means of translations into distinct behavioral formalisms such as automatas or Petri nets. In contrast to translational approaches we propose an operational approach. Its principle is to identify which elementary communication actions can be immediately executed, and then to compute, for every such action, a new interaction representing the possible continuations to its execution. We also define an algorithm for checking the validity of execution traces (i.e. whether or not they belong to an interaction's semantics). Algorithms for semantic computation and trace validity are analyzed by means of experiments.Comment: 18 pages of contents and 2 pages for references, 10 figures. Published in ETAPS-FASE2020 : "23rd International Conference on Fundamental Approaches to Software Engineering" in the "research papers" categor

Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family

Aims: The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny. Methods and Results: We used a high-performance liquid chromatography– tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25–5000 nmol l-1 . Conclusions: This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles. Significance and Impact of the Study: The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains

Current status of turbulent dynamo theory: From large-scale to small-scale dynamos

Several recent advances in turbulent dynamo theory are reviewed. High resolution simulations of small-scale and large-scale dynamo action in periodic domains are compared with each other and contrasted with similar results at low magnetic Prandtl numbers. It is argued that all the different cases show similarities at intermediate length scales. On the other hand, in the presence of helicity of the turbulence, power develops on large scales, which is not present in non-helical small-scale turbulent dynamos. At small length scales, differences occur in connection with the dissipation cutoff scales associated with the respective value of the magnetic Prandtl number. These differences are found to be independent of whether or not there is large-scale dynamo action. However, large-scale dynamos in homogeneous systems are shown to suffer from resistive slow-down even at intermediate length scales. The results from simulations are connected to mean field theory and its applications. Recent work on helicity fluxes to alleviate large-scale dynamo quenching, shear dynamos, nonlocal effects and magnetic structures from strong density stratification are highlighted. Several insights which arise from analytic considerations of small-scale dynamos are discussed.Comment: 36 pages, 11 figures, Spa. Sci. Rev., submitted to the special issue "Magnetism in the Universe" (ed. A. Balogh

Revising the Language Map of Korea

As linguists develop a deeper understanding of the properties of individual varieties of speech, they often find it necessary to reclassify dialects as independent languages, based on the criterion of intelligibility. This criterion is applied here to Jejueo, the traditional variety of speech used on Jeju Island, a province of the Republic of Korea. Although Jejueo has long been classified as a nonstandard dialect of Korean, evidence from an intelligibility experiment shows that it is not comprehensible to monolingual speakers of Korean and therefore should be treated as a separate language, in accordance with the usual practice within linguistics. This finding calls for a revision to the standard language map of Kore