An Ecological Study of Cladophora glomerate (Chlorophyceae) near Dallas

Cladophora glomerata, a green alga of the order Ulotrichales, family Cladophoraceae, is characterized by dich tomous branching, with ramuli ending in dense terminal fascicles…. Between October, 1949 and July, 1950, we collected this species in the Dallas area from Elm Fork of the Trinity River, and Bachman\u27s, White Rock, and Ten Mile creeks

The length of homology required for gene targeting in embryonic stem cells

Homologous recombination has been used to introduce site-specific mutations into murine embryonic stem (ES) cells with both insertion and replacement vectors. In this study, we compared the frequency of gene targeting with various lengths of homology and found a dramatic increase in targeting with an increase in homology from 1.3 to 6.8 kb. We examined in detail the relationship between the length of homology and the gene-targeting frequency for replacement vectors and found that a critical length of homology is needed for targeting. Adding greater lengths of homology to this critical length has less of an effect on the targeting frequency. We also analyzed the lengths of homology necessary on both arms of the vector for gene replacement events and found that 472 bp of homology is used as efficiently as 1.2 kb in the formation and resolution of crossover junctions

Preparation of Neutron-activated Xenon for Liquid Xenon Detector Calibration

We report the preparation of neutron-activated xenon for the calibration of liquid xenon (LXe) detectors. Gamma rays from the decay of xenon metastable states, produced by fast neutron activation, were detected and their activities measured in a LXe scintillation detector. Following a five-day activation of natural xenon gas with a Cf-252 (4 x 10^5 n/s) source, the activities of two gamma ray lines at 164 keV and 236 keV, from Xe-131m and Xe-129m metastable states, were measured at about 95 and 130 Bq/kg, respectively. We also observed three additional lines at 35 keV, 100 keV and 275 keV, which decay away within a few days. No long-lifetime activity was observed after the neutron activation.Comment: to be published in NIM A, corrected typos in Table 1 and Fig.6 of the previous versio

Serially-regulated biological networks fully realize a constrained set of functions

We show that biological networks with serial regulation (each node regulated by at most one other node) are constrained to {\it direct functionality}, in which the sign of the effect of an environmental input on a target species depends only on the direct path from the input to the target, even when there is a feedback loop allowing for multiple interaction pathways. Using a stochastic model for a set of small transcriptional regulatory networks that have been studied experimentally, we further find that all networks can achieve all functions permitted by this constraint under reasonable settings of biochemical parameters. This underscores the functional versatility of the networks.Comment: 9 pages, 3 figure

Noise-induced dynamics in bistable systems with delay

Noise-induced dynamics of a prototypical bistable system with delayed feedback is studied theoretically and numerically. For small noise and magnitude of the feedback, the problem is reduced to the analysis of the two-state model with transition rates depending on the earlier state of the system. In this two-state approximation, we found analytical formulae for the autocorrelation function, the power spectrum, and the linear response to a periodic perturbation. They show very good agreement with direct numerical simulations of the original Langevin equation. The power spectrum has a pronounced peak at the frequency corresponding to the inverse delay time, whose amplitude has a maximum at a certain noise level, thus demonstrating coherence resonance. The linear response to the external periodic force also has maxima at the frequencies corresponding to the inverse delay time and its harmonics.Comment: 4 pages, 4 figures, submitted to Physical Review Letter

Cell cycle-dependent variations in protein concentration

Computational modeling of biological systems has become an effective tool for analyzing cellular behavior and for elucidating key properties of the intricate networks that underlie experimental observations. While most modeling techniques rely heavily on the concentrations of intracellular molecules, little attention has been paid to tracking and simulating the significant volume fluctuations that occur over each cell division cycle. Here, we use fluorescence microscopy to acquire single cell volume trajectories for a large population of Saccharomyces cerevisiae cells. Using this data, we generate a comprehensive set of statistics that govern the growth and division of these cells over many generations, and we discover several interesting trends in their size, growth and protein production characteristics. We use these statistics to develop an accurate model of cell cycle volume dynamics, starting at cell birth. Finally, we demonstrate the importance of tracking volume fluctuations by combining cell division dynamics with a minimal gene expression model for a constitutively expressed fluorescent protein. The significant oscillations in the cellular concentration of a stable, highly expressed protein mimic the observed experimental trajectories and demonstrate the fundamental impact that the cell cycle has on cellular functions

Dynamically Driven Renormalization Group Applied to Sandpile Models

The general framework for the renormalization group analysis of self-organized critical sandpile models is formulated. The usual real space renormalization scheme for lattice models when applied to nonequilibrium dynamical models must be supplemented by feedback relations coming from the stationarity conditions. On the basis of these ideas the Dynamically Driven Renormalization Group is applied to describe the boundary and bulk critical behavior of sandpile models. A detailed description of the branching nature of sandpile avalanches is given in terms of the generating functions of the underlying branching process.Comment: 18 RevTeX pages, 5 figure

Comments on Non-Commutative Phenomenology

It is natural to ask whether non-commutative geometry plays a role in four dimensional physics. By performing explicit computations in various toy models, we show that quantum effects lead to violations of Lorentz invariance at the level of operators of dimension three or four. The resulting constraints are very stringent.Comment: Correction of an error in the U(1) and U(N) calculation leads to stronger limits than those given previously Clarifying comments and reference adde