Nosocomial infections and their control strategies

Nosocomial infections are also known as hospital-acquired/associated infections. National Healthcare Safety Network along with Centers for Disease Control for surveillance has classified nosocomial infection sites into 13 types with 50 infection sites, which are specific on the basis of biological and clinical criteria. The agents that are usually involved in hospital-acquired infections include Streptococcus spp., Acinetobacter spp., enterococci, Pseudomonas aeruginosa, coagulase-negative staphylococci, Staphylococcus aureus, Bacillus cereus, Legionella and Enterobacteriaceae family members, namely, Proteus mirablis, Klebsiella pneumonia, Escherichia coli, Serratia marcescens. Nosocomial pathogens can be transmitted through person to person, environment or contaminated water and food, infected individuals, contaminated healthcare personnel's skin or contact via shared items and surfaces. Mainly, multi-drug-resistant nosocomial organisms include methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, Pseudomonas aeruginosa and Klebsiella pneumonia, whereas Clostridium difficile shows natural resistance. Excessive and improper use of broad-spectrum antibiotics, especially in healthcare settings, is elevating nosocomial infections, which not only becomes a big health care problem but also causes great economic and production loss in the community. Nosocomial infections can be controlled by measuring and comparing the infection rates within healthcare settings and sticking to the best healthcare practices. Centers for Disease Control and Prevention provides the methodology for surveillance of nosocomial infections along with investigation of major outbreaks. By means of this surveillance, hospitals can devise a strategy comprising of infection control practices

A cost effective preparative thin layer chromatography cleanup method for high performance liquid chromatography analysis of aflatoxins B1, B2 and G2

Aflatoxins are the by-products of fungal metabolism and common contaminants in feed. To keep their level below permissible limits, various assays have been developed. Currently, High Performance Liquid Chromatography (HPLC) with fluorescence detection of toxins is most widely used method but the cleanup methods requiring specific gadgets have increased the cost of the assay. Thin Layer Chromatography (TLC) is effective and economical but it only gives semi quantitative determination for aflatoxin.This study explores the preparative potential of TLC as cleanup method for HPLC analysis of toxin. Standard aflatoxins solutions containing 10 ng of B1, 2.5 ng of B2 and 2.5 ng of G2 were spotted on silica plates and then extracted using chloroform and acetone (4:1). The extracted toxins were resolved and quantified on HPLC using fluorescent detection. The results showed 81, 79 and 60% mean recovery of aflatoxin B1, B2 and G2, respectively. This method was proved equivocally comparable to other methods of aflatoxins cleanup and thus can be used as an alternative cost effective cleanup method

A methodology for flexibility analysis of pipeline systems

Pipeline systems serve a crucial role in an effective transport of fluids to the designated location for medium to long span of distances. Owing to its paramount economic significance, pipeline design field have undergone extensive development over the past few years for enhancing the optimization and transport efficiency. This research paper attempts to propose a methodology for flexibility analysis of pipeline systems through employing contemporary computational tools and practices. A methodical procedure is developed, which involves modeling of the selected pipeline system in CAESAR II followed by the insertion of pipe supports and restraints. The specific location and selection of the inserted supports is based on the results derived from the displacement, stress, reaction, and nozzle analysis of the concerned pipeline system. Emphasis is laid on the compliance of the design features to the leading code of pipeline transportation systems for liquid and slurries, ASME B31.4. The discussed procedure and approach can be successfully adjusted for the analysis of various other types of pipeline system configuration. In addition to the provision of systematic flow in analysis, the method also improves efficient time-saving practices in the pipeline stress analysis

The Response of Land Surface Temperature to the Changing Land-Use Land-Cover in a Mountainous Landscape under the Influence of Urbanization: Gilgit City as a case study in the Hindu Kush Himalayan Region of Pakistan

With growing urbanization in mountainous landscapes, the built-up areas dominate other land use classesresulting in increased land surface temperature (LST). Gilgit city in northern Pakistan has witnessed tremendousurban growth in the recent past decades. It is anticipated that this growth will exponentially increase in the nearfuture because of the China-Pakistan Economic Corridor (CPEC) initiatives, as this city happens to be thecommercial hub of the northern region of Pakistan. The objective of present study is to explore the influence ofland use and land cover variations on LST and to evaluate the relationship between LST with normalizeddifference vegetation index (NDVI), normalized difference water index (NDWI), and normalized difference built -up index (NDBI) values. This study is carried out on data from Google earth and three Landsat images (Landsat 5-TM, Landsat 7-ETM, and Landsat OLI_TIRS-8) during the period from 1992, 2004 and 2016. Land use/coverclasses are determined through supervised classification and LST maps are created using the Mono -windowalgorithm. The accuracy assessment of land use/cover classes is carried out comparing Google Earth digitizedvector for the periods of 2004 and 2016 with Landsat classified images. Further, NDVI, NDBI, and NDWI mapsare computed from images for years 1992, 2004, and 2016. The relationships of LST with NDVI, NDBI, andNDWI are computed using Linear Regression analysis. The results reveal that the variations in land use and landcover play a substantial role in LST variability. The maximum temperatures are connected with built -up areas andbarren land, ranging from 48.4°C, 50.7°C, 51.6°C, in 1992, 2004, and 2016, respectively. Inversely, minimumtemperatures are linked to forests and water bodies, ranging from 15.1°C, 16°C, 21.6°C, in 1992, 2004, and 2016respectively. This paper also results that NDBI correlates positively with high temperatures, whereas NDVI andNDWI associate negatively with lesser temperatures. The study will support to policymakers and urban planners tostrategize the initiatives for eco-friendly and climate-resilient urban development in fragile mountainouslandscapes

Impact of Audit Quality on Real Earnings Management: Moderating Role of Corporate Governance

Purpose: This study aims to examine the effectiveness of audit quality in restricting earnings management in the presence of country’s governance system. Methodology: The data was collected from 195 Pakistani-based and 150 UK based non-financial companies. The sampling period is ten years from 2010 to 2019. To test the hypotheses, the Generalized Method of Moments was applied. Findings: The results showed that firms switch from accrual earnings management to real earnings management in developed economies which are characterized by strong governance mechanism. Moreover, the negative association between governance mechanism and earnings management is increased in the presence of Big-4 auditor. Conclusion: It is concluded that audit quality restricts the firms to use real earnings management especially in those countries where governance mechanism is strong

Anticonvulsant, Antimicrobial and Cytotoxic Activities of Berberis calliobotrys Aitch ex Koehne (Berberidaceae)

Purpose: To evaluate the anticonvulsant, antimicrobial and cytotoxic activities of Berberis calliobotrys.Methods: The powdered plant material (10 kg) was extracted thrice with methanol (3 × 12 L) by dipping for seven days. The methanol extract was concentrated to dryness under reduced pressure, and then successively fractionated with solvents of different polarity, including n-hexane, chloroform, ethyl acetate and n-butanol. The anticonvulsant effect of the extract and fractions (at oral doses 500 and 1000 mg/kg) was studied against picrotoxin-, pentylenetetrazole (PTZ)- and strychnine-induced seizures in Swiss albino mice of either sex divided into 12 groups (n = 6). Diazepam was used as standard drug. Antimicrobial activity of the extract against Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus as well as against Candida albicans, Penicillium notatum was conducted by disc diffusion method and minimum inhibitory concentration (MIC). Cytotoxicity of the extract/fractions was analyzed by  haemolytic method while the phenolic compounds present in the ethyl acetate fraction of the plant were determined by high performance liquid chromatography (HPLC).Results: The extract and its ethyl acetate and n-butanol fractions showed maximum response against drug-induced convulsions and provided 100 % protection to animals at both doses. They also showed zones of inhibition of 27.00 ± 2.51, and 22.00 ± 2.51 mm against all bacterial and fungal strains, respectively, especially Staphylococcus aureus. The methanol extract and ethyl acetate fraction also showed high MIC against all bacterial and fungal strains. Cytotoxicity data from hemolytic assay indicate that the extract/fractions are safe. The highest amount of phenolic found was chlorogenic acid (84.44 ± 0.06 ppm).Conclusion: The plant is thus a potential source of new lead compounds for the development of new clinically effective anticonvulsant and antimicrobial compounds.Keywords: Berberis calliobotrys, Anticonvulsant, Antimicrobial, Haemolytic, Phenolics, Chlorogenic aci

Assessment of Optimal Production Through Assembly Line-Balancing and Product-Mix Flexibility

Timely accomplishment of production targets is a challenging task in low volume–high variety environment. Assessment of the manufacturing flexibility of a production system assists in achieving the desired objectives. In this research, the operational flexibility of a production system is investigated which operates under the low-volume high-variety production scenario. Prospective dimensions of theproduction flexibility are studied to analyze its interface with the integrated functional units. It was analyzed that with a low-volumeoperational flexibility (OF) varies rationally despite high job varieties. Line-balancing and queuing techniques are applied to ascertain theoptimum productivity. A sensitivity analysis is also performed to evaluate the critical parameters that affect the OF and productivity level.OF index of the production system was estimated by means of the optimized production parameters. A comparative analysis is performedto evaluate the flexibility in conventional and flexible production cells. Analytical and computational results show a close approximationand validate the implemented schemes

Inventory Management and Its Effects on Customer Satisfaction

This study examines how inventory management puts positive impact on customer satisfaction and how easily we can check the performance. It also helps retailers to put their inventories in proper order which tells them about demand and supply of their inventories. Proper inventory management system reduces the risk of short of inventories which reduce the cost of lost customers. The objective of the study is to minimize the risk of dissatisfaction of customers and found how to sustain customer satisfaction with the help of proper inventories system. This paper also outlines significant relationship between Customer needs, Quality with variable of prime interest. Poor association has been found between performance and customer satisfaction

An agent based architecture for cognitive spectrum management

In the recent years, wireless technologies and devices have progressed dramatically that has augmented the demand for electromagnetic spectrum. Some research work showed that spectrum access and provision to user is not possible due to shortage of spectrum but federal communication commission refused to accept this theory and indicated that the spectrum is available since most of the frequency bands are underutilized. In order to allow the use of these frequency bands without interference, cognitive radio was proposed that characterizes the growing intelligence of radio systems can adapt to the radio environment, allowing opportunistic usage and sharing with the existing uses of spectrum. To take this concept a step further, we propose to use intelligent agent for spectrum management in the context of cognitive radio in this paper. In our proposed architecture, agents are embedded in the radio devices that coordinate their operations to benefit from network and avoid interference with the primary user. Agents carry a set of modules to gather information about the terminal status and the radio environment and act accordingly to the constraints of the user application