Some hematological and biochemical changes in blood serum of Beluga (Huso huso) after chronic exposure to diazinon

Hematological and biochemical changes were studied in beluga (Huso huso) weighing 450±50g in the presence of b,1-3, D-glucan as a single intraperitoneal injection (0.3mg/kg body weight) once, and thereafter a chronic exposure to organophosphate, diazinon at concentration of 1.5 mg/L as a continuous bath for 63 days at 22±1ºC. Results of erythrocyte profile in fish treated with glucan, but without diazinon, generally showed significant higher values of RBC (P0.05) in the values of HCT, Hb, MCH and MCHC between the two groups. Also, fish treated with glucan, but without diazinon bath, showed significant higher values of RBC than that untreated fish up to 3 weeks post-treatment (P0.05). Furthermore, fish treated with diazinon showed higher levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) than other three groups for 14-28 days post-treatment and then they decreased to the lower level for the rest of experiment. Also, fish treated with glucan-diazinon showed insignificant higher levels of these enzymes up to 21 days post-exposure compare to fish treated with glucan but without diazinon bath (P>0.05). However, such levels reduced to a lower level in this group for the rest of experiment (P>0.05). Also, fish treated with glucan-diazinon showed generally lower and higher levels of total protein and glucose concentrations in blood plasma, respectively compared to fish treated with glucan but without diazinon bath (P<0.05)

Radiation characteristic of slots on conducting circular cylindercovered by eccentric dielectric cylinder

The radiation characteristics of axial slots on a circular conducting cylinder covered by an eccentric dielectric cylinder are investigated. A dual infinite-series solution based on the boundary-value method is obtained with the aid of the addition theorem of Bessel functions. The dual infinite series involved in the solution is then properly truncated to generate numerical results. Comparison with published data for concentric cylinders shows excellent agreement. Several interesting radiation characteristics corresponding to new geometries of the coated slot cylindrical antenna are presente

Radiation characteristic of slots on conducting circular cylindercovered by eccentric dielectric cylinder

The radiation characteristics of axial slots on a circular conducting cylinder covered by an eccentric dielectric cylinder are investigated. A dual infinite-series solution based on the boundary-value method is obtained with the aid of the addition theorem of Bessel functions. The dual infinite series involved in the solution is then properly truncated to generate numerical results. Comparison with published data for concentric cylinders shows excellent agreement. Several interesting radiation characteristics corresponding to new geometries of the coated slot cylindrical antenna are presente

Classification of visualization exudates fundus images results using support vector machine

This paper classifies the characteristics of normal and exudates fundus images by determine its accuracy for diagnostic purposes. Image normalization was performed on 149 fundus images (81 normal and 68 exudates) from MESSIDOR databases to standardize the colours in the fundus images. The OD removed fundus image and fundus image with the exudates areas removed. The SVM1 classifier was applied to 30 test fundus images to determine the best optimal parameter. The kernel function settings; linear, polynomial, quadratic and RBF have an effect on the classification results. For SVM1, the best parameter in classifying pixels is linear kernel function. The visualization results using CAC and radar chart are classified using ts accuracy. It has proven to discriminated exudates and non exudates pixels in fundus image using linear kernel function of SVM1 to diagnose DR.Keywords: Diabetic retinopathy (DR); Optic disc (OD); Support Vector Machine (SVM); AC); Radial Basis Function (RBF)

Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water

Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157:H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g or mL, respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water

Forecasting rainfed sorghum yield using satellite-derived vegetation indices with limited ground-based information in Gadarif region, eastern Sudan

A practical crop growth and yield monitoring system based on satellite data is required and fundamental not only for precision farming, but also very useful for global food security enhancement. This study was performed to determine the optimal vegetation index and also to identify the best time for making a reliable crop yield forecast in one of the major sorghum-growing region (Gedarif State, Sudan). The study was also aimed to develop a simple yield prediction model which was later validated using an official yield data acquired during 2013 and 2014 cropping seasons from the Department of Information System and Statistical Analysis of the State Ministry of Agriculture, Gedarif State. The study used NASA’s multi-temporal MODerate resolution Imaging Spectroradiometer (MODIS) land products with limited ground information. Relationship between sorghum yield and crop reflectance indicated that normalized difference vegetation index (NDVI) at the third dekad of September (Sep.III) is the most appropriate to develop sorghum yield prediction model with higher R2 value of 0.77 (p&lt;0.05) compared to other vegetation indices (normalized ratio vegetation index, NRVI and soil-adjusted vegetation index, SAVI). The plotting of estimated yield against actual yield during 2013 and 2014 cropping seasons revealed strong positive and linear correlations (R2 = 0.64 (p=0.06) and 0.74 (p&lt;0.05), respectively with average R2 = 0.71 (p&lt;0.001) for both seasons. This study concluded that a good prediction of rainfed sorghum yield could be achieved more than 30 days before harvesting with quick, accurate and cost-effective method compared to traditional field surveys

Some hematological and biochemical changes in blood serum of grass carp (Ctenopharyngodon idella ) vaccinated with Aeromonas hydrophila following exposure to sublethal concentration of diazinon

Diazinon is commonly used for pest control in the agricultural fields in north of Iran. This study was conducted to determine the chronic toxicity of organophosphorous pesticide (Diazinon) in vaccinated fish and its effects on some hematological parameters and biochemical blood plasma profiles of Grass Carp (Ctenopharyngodon idella). This experiment was carried out in three groups. The first group was vaccinated and exposed to diazinon (group A) while the second group was vaccinated and bathed with PBS bath (group B). The remaining fish were used as unvaccinated fish and were kept in clean water separately (group C). Diazinon was applied at concentrations of 2 mg/L for 12 hours since the experiments were initiated. The experimental groups (A and B) showed significantly lower values (p < 0.05) of erythrocyte count, haemoglobin content, haematocrit, leucocytes, Lymphocyte, myelocyte and monocyte, as well as in alkaline phosphatases, alanine aminotransferase, aspartate aminotransferase and, lactate dehydrogenase compared to the control group (C). Values of MCV, MCH and MCHC of experimental species (A and B) were compared to the control group (C). The results of examinations of the biochemical blood plasma profile indicate a marked neurotoxic effect of diazinon in fishes. Changes in values of both erythrocyte and leukocyte profile after exposure to diazinon-based preparation may be referred to disruption of haematopoiesis as well as to a decrease on non-specific immunity of the fish

Detection of asphyxia in infants using deep learning Convolutional Neural Network (CNN) trained on Mel Frequency Cepstrum Coefficient (MFCC) features extracted from cry sounds

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Induction of cryptic antifungal pulicatin derivatives from <i>Pantoea agglomerans</i> by microbial co-culture

Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment