7 research outputs found
Multilabel land cover aerial image classification using convolutional neural networks
Classifying the remote sensing images requires a deeper understanding of remote sensing imagery, machine learning classification
algorithms, and a profound insight into satellite imagesâ know-how properties. In this paper, a convolutional neural network (CNN) is
designed to classify the multispectral SAT-4 images into four classes: trees, grassland, barren land, and others. SAT-4 is an airborne
dataset that captures the images in 4 bands (R, G, B, infrared). The proposed CNN classifier learns the imageâs spectral and spatial
properties fromthe ground truth samples provided. The contribution of this paper is three-fold. (1) A classification framework for feature
extraction and normalization is built. (2) Nine different architectures of models are built, and multiple experiments are conducted to
classify the images. (3) A deeper understanding of the image structure and resolution is captured by varying different optimizers inCNN.
The correlation between images of varying classes is identified. The experimental study shows that vegetation health is predicted most
accurately by the proposed CNN models. It significantly differentiates the grassland vegetation from tree vegetation, which is better than
other classical methods. The tabulated results show that a state-of-the-art analysis is done to learn varying landcover classification models
New Signatures of Bio-Molecular Complexity in the Hypervelocity Impact Ejecta of Icy Moon Analogues
Impact delivery of prebiotic compounds to the early Earth from an impacting comet is considered to be one of the possible ways by which prebiotic molecules arrived on the Earth. Given the ubiquity of impact features observed on all planetary bodies, bolide impacts may be a common source of organics on other planetary bodies both in our own and other solar systems. Biomolecules such as amino acids have been detected on comets and are known to be synthesized due to impact-induced shock processing. Here we report the results of a set of hypervelocity impact experiments where we shocked icy mixtures of amino acids mimicking the icy surface of planetary bodies with high-speed projectiles using a two-stage light gas gun and analyzed the ejecta material after impact. Electron microscopic observations of the ejecta have shown the presence of macroscale structures with long polypeptide chains revealed from LCMS analysis. These results suggest a pathway in which impact on cometary ices containing building blocks of life can lead to the synthesis of material architectures that could have played a role in the emergence of life on the Earth and which may be applied to other planetary bodies as well. View Full-Tex
Influence of Phosphorus and Sulphur on Growth and Yield of Sunflower (Helianthus annuus L.)
A field experiment was conducted during Kharif 2022 at Crop Research Farm, Department of Agronomy, Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj (U.P). To determine the âInfluence of phosphorus and sulphur on growth and yield of sunflower (Helianthus annuus L.)â, The results showed that treatment 9 [Phosphorus (60 kg/ha) + Sulphur 25 kg/ha] recorded significantly higher plant height (123.10 cm), higher plant dry weight (91.03 g). Where as, higher number of seeds/capitulum (412.00), higher test weight (50.50 g), higher seed yield (1.25 t/ha), higher Stalk yield (3.44 t/ha), higher oil content (40.37%), was recorded in treatment 9 [Phosphorus (60 kg/ha) + Sulphur 25 kg/ha). Similarly, maximum gross returns (83,440.00 INR/ha), higher net returns (55,851.25 INR/ha), and highest benefit cost ratio (2.02) was also recorded in treatment 9 [Phosphorus (60kg/ha) +Sulphur (25kg/ha)], as compared to other treatments
Benzothiazole and Chromone Derivatives as Potential ATR Kinase Inhibitors and Anticancer Agents
Despite extensive studies and the great variety of existing anticancer agents, cancer treatment remains an aggravating and challenging problem. Therefore, the development of novel anticancer drugs with a better therapeutic profile and fewer side effects to combat this persistent disease is still necessary. In this study, we report a novel series of benzothiazole and chromone derivatives that were synthesized and evaluated for their anticancer activity as an inhibitor of ATR kinase, a master regulator of the DDR pathway. The cell viability of a set of 25 compounds was performed using MTT assay in HCT116 and HeLa cell lines, involving 72 h incubation of the compounds at a final concentration of 10 µM. Cells incubated with compounds 2c, 7h and 7l were found to show viability ≤50%, and were taken forward for dose–response studies. Among the tested compounds, three of them (2c, 7h and 7l) showed higher potency, with compound 7l exhibiting the best IC50 values in both the cell lines. Compounds 2c and 7l were found to be equally cytotoxic towards both the cell lines, namely, HCT116 and HeLa, while compound 7h showed better cytotoxicity towards HeLa cell line. For these three compounds, an immunoblot assay was carried out in order to analyze the inhibition of phosphorylation of Chk1 at Ser 317 in HeLa and HCT116 cells. Compound 7h showed inhibition of pChk1 at Ser 317 in HeLa cells at a concentration of 3.995 µM. Further analysis for Chk1 and pChk1 expression was carried out in Hela cells by treatment against all the three compounds at a range of concentrations of 2, 5 and 10 µM, wherein compound 7h showed Chk1 inhibition at 2 and 5 µM, while pChk1 expression was observed for compound 7l at a concentration of 5 µM. To support the results, the binding interactions of the compounds with the ATR kinase domain was studied through molecular docking, wherein compounds 2c, 7h and 7l showed binding interactions similar to those of Torin2, a known mTOR/ATR inhibitor. Further studies on this set of molecules is in progress for their specificity towards the ATR pathway
New Signatures of Bio-Molecular Complexity in the Hypervelocity Impact Ejecta of Icy Moon Analogues
Impact delivery of prebiotic compounds to the early Earth from an impacting comet is considered to be one of the possible ways by which prebiotic molecules arrived on the Earth. Given the ubiquity of impact features observed on all planetary bodies, bolide impacts may be a common source of organics on other planetary bodies both in our own and other solar systems. Biomolecules such as amino acids have been detected on comets and are known to be synthesized due to impact-induced shock processing. Here we report the results of a set of hypervelocity impact experiments where we shocked icy mixtures of amino acids mimicking the icy surface of planetary bodies with high-speed projectiles using a two-stage light gas gun and analyzed the ejecta material after impact. Electron microscopic observations of the ejecta have shown the presence of macroscale structures with long polypeptide chains revealed from LCMS analysis. These results suggest a pathway in which impact on cometary ices containing building blocks of life can lead to the synthesis of material architectures that could have played a role in the emergence of life on the Earth and which may be applied to other planetary bodies as well
Deconstructing Noncovalent Kelch-like ECH-Associated Protein 1 (Keap1) Inhibitors into Fragments to Reconstruct New Potent Compounds
Targeting the proteinâprotein interaction (PPI) between nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) is a potential therapeutic strategy to control diseases involving oxidative stress. Here, six classes of known small-molecule Keap1âNrf2 PPI inhibitors were dissected into 77 fragments in a fragment-based deconstruction reconstruction (FBDR) study and tested in four orthogonal assays. This gave 17 fragment hits of which six were shown by X-ray crystallography to bind in the Keap1 Kelch binding pocket. Two hits were merged into compound 8 with a 220â380-fold stronger affinity (K = 16 ÎźM) relative to the parent fragments. Systematic optimization resulted in several novel analogues with Ki values of 0.04â0.5 ÎźM, binding modes determined by X-ray crystallography, and enhanced microsomal stability. This demonstrates how FBDR can be used to find new fragment hits, elucidate important ligandâprotein interactions, and identify new potent inhibitors of the Keap1âNrf2 PPI