Genetic Influences on the Response to Neuromodulation in Craving Behaviors

Obesity and eating disorders are highly prevalent in the United States. People who suffer from obesity and/or eating disorders face serious health consequences and even death. Current treatments are not effective as recovery rates are low, so there is a dire need for an effective treatment for obesity and eating disorders. There have been studies investigating the use of transcranial direct current stimulation (tDCS) and transcranial magnetic stimulation (TMS) of the dorsolateral prefrontal cortex (DLPFC) as a means of treatment for these people. While findings show promise, there is much variability. The goal of this study is to further prior work by evaluating the ability of tDCS and TMS to modulate food cravings and impulsivity. Additionally, genetic factors will be analyzed for their use in predicting neuromodulation efficacy. For this study, we recruited a total of 30 participants who were assigned to either the tDCS group (n=15) or the TMS group (n=15). Each participant came in for a total of three visits where they completed a series of questionnaires, underwent sham or active neuromodulation, completed a food preference task, impulsivity task, and had blood drawn. Preliminary results demonstrate that tDCS and TMS can reduce wanting to eat in general. The reduction in wanting to eat may be through modifications of feelings of lack of control. More specifically, neuromodulation can selectively decrease the appeal of high calorie foods through activation of the dorsolateral prefrontal cortex. Further, analyzing genetic factors can help predict who will respond best to neuromodulation

Application of chicken microarrays for gene expression analysis in other avian species

BACKGROUND: With the threat of emerging infectious diseases such as avian influenza, whose natural hosts are thought to be a variety of wild water birds including duck, we are armed with very few genomic resources to investigate large scale immunological gene expression studies in avian species. Multiple options exist for conducting large gene expression studies in chickens and in this study we explore the feasibility of using one of these tools to investigate gene expression in other avian species. RESULTS: In this study we utilised a whole genome long oligonucleotide chicken microarray to assess the utility of cross species hybridisation (CSH). We successfully hybridised a number of different avian species to this array, obtaining reliable signals. We were able to distinguish ducks that were infected with avian influenza from uninfected ducks using this microarray platform. In addition, we were able to detect known chicken immunological genes in all of the hybridised avian species. CONCLUSION: Cross species hybridisation using long oligonucleotide microarrays is a powerful tool to study the immune response in avian species with little available genomic information. The present study validated the use of the whole genome long oligonucleotide chicken microarray to investigate gene expression in a range of avian species

Reflecting on childhood and child agency in history

In today’s fast changing and unpredictable world we tend to rely upon children’s agency to ensure their survival following traumatic events. Because of this, parents and teachers need to be conversant with ways of encouraging children’s agency to support their health and efficacy throughout the lifespan. This paper takes the form of a literature review covering historical and recent developments in the field of childhood, child abuse and child agency. It offers the findings of a qualitative document study that aimed to explore how childhood and child agency have been conceptualized historically in the Western world. It begins with a summary of theories and typologies of childhood throughout history, exploring the unique social and historical concepts in which theories of childhood and child agency developed. Furthermore, a paradigm shift in Western society towards women achieving agency is revealed. With this came greater attention to the child, especially the abused child. Focusing on abuse to which children have been exposed, this study looks at child agency and how it can be actualized for children’s wellbeing. Research findings make a strong case for the arts to provide valuable tools of resilience for the developing child. Arts-based activities have been increasingly appreciated as providing a voice for traumatized or abused children. Therefore, the findings of this study into agency and child abuse were distilled into a poem to demonstrate the potential impact on a child and to help adults better understand the historically perceived voiceless victim. The poem, as seen from the perspective of the child, offers a distinctive contribution to the literature on child abuse. Key findings of this study are discussed, including the importance of increasing understanding of children’s agency. It is argued that a shift in childrearing, incorporated child protection laws and agentic experiences build children’s confidence to challenge the traditional adult-child power relationship. The results of this research help to contribute to teacher knowledge about the intricate background to child abuse and child agenc

Sustainable organic plant breeding: Final report - a vision, choices, consequences and steps

In general, the characteristics of organic varieties - and by extension of organic plant breeding - differ from that of conventional breeding systems and conventional varieties. Realising an organic plant breeding system and subsequently steering it to meet changing demands is no less than a mammoth task. The many actions to be undertaken can be divided into short-term commercial and scientific activities, and longer or long-term commercial and scientific activities. Action must be taken in the short-term to ensure adequate quantities of organically propagated plants and seed. This is vital in consideration of Regulation 2092/91/EC which states that, as of 1 January 2000, all propagating material used in organic production must be of organic origin. Additional measures are needed to accelerate the development of organically propagated varieties. Within the breeding sector, variety groups should be established to streamline communication in the chain. Variety groups should have a large contingent of farmers, as well as representatives from the trade branch and breeders. Members should communicate intensively with each other, share experiences, and participate in trials and variety assessments. Questions, wishes and bottlenecks could be recorded by variety groups and passed on to other parties in the chain. The practical details of the plant health concept which is at the basis of organic breeding must be worked out (operationalised). This will require scientific research, for example on: root development and mineral absorption efficiency weed suppressive capacity in situ versus ex situ maintenance resistance breeding in combination with cultivation measures seed-transmitted diseases adaptive capacity alternatives for growth stimulants, silver nitrate and silver thiosulfate in the cultivation of cucumbers and pickles Such research should be carried out by academic institutions (such as Wageningen University and Research Centre) in collaboration with Louis Bolk Institute, Stichting Zaadgoed and private companies. A platform should be established to make an inventory of problems and priorities and to develop research proposals. Farmers could contribute their ideas to the platform through the variety groups. Conclusion A plant breeding system for organic production should be based on the organic concept of plant health and on the organic position on chain relationships. As the total land area under organic production is still relatively small, it is unlikely that commercial breeders will make large investments to develop organic breeding programmes without financial support from other parties, i.e. the government. In this early stage, it is vital that the government provides generous funding and plays an active enabling role. We hope that the action plan to stimulate organic plant breeding, as requested by Parliament, will dovetail with the activities described above

Mouse hepatitis virus neurovirulence: evidence of a linkage between S glycoprotein expression and immunopathology.

Differences in disease outcome between the highly neurovirulent MHV-JHM and mildly neurovirulent MHV-A59 have been attributed to variations within the spike (S) glycoprotein. Previously, we found that MHV-JHM neurovirulence was marked by diminished expression of interferon-gamma (IFN-gamma) mRNA and a reduced presence of CD8 T cells in the CNS concomitant with heightened macrophage inflammatory protein (MIP)-1 transcript levels and greater macrophage infiltration relative to MHV-A59 infection. Here, the ability of the S and non-spike genes to regulate these immune responses was evaluated using chimeric viruses. Chimeric viruses WTR13 and S4R22 were made on MHV-A59 variant backgrounds and, respectively, contained the S gene of MHV-A59 and MHV-JHM. Unexpectedly, genes other than S appeared to modulate events critical to viral replication and survival. Unlike unresolving MHV-JHM infections, the clearance of WTR13 and S4R22 infections coincided with strong IFN-gamma transcription and an increase in the number of CD8 T cells infiltrating into the CNS. However, despite the absence of detectable viral titers, approximately 40% of S4R22-infected mice succumbed within 3 weeks, indicating that the enhanced mortality following S4R22 infection was not associated with high viral titers. Instead, similar to the MHV-JHM infection, reduced survival following S4R22 infection was observed in the presence of elevated MIP-1alpha and MIP-1beta mRNA accumulation and enhanced macrophage numbers within infected brains. These observations suggest that the S protein of MHV-JHM influences neurovirulence through the induction of MIP-1alpha- and MIP-1beta-driven macrophage immunopathology

Effective fuel temperature of wwer-1000

The main temperature characteristics of a pressurized water reactor are distinguished, supporting its safety and reliable operation. The special role of the uranium fuel effective temperature is emphasized and the accuracy of the analytical determination of the power effect is increased. The calculation of the temperature distribution along the radius of the fuel rod was carried out taking into account the temperature dependence of the thermal conductivity UO2. The design procedure was corrected for using the Finca-Ronchi dependence for the thermal conductivity of 95% density of the theoretical one

Time-resolved broadband analysis of slow-light propagation and superluminal transmission of electromagnetic waves in three-dimensional photonic crystals

A time-resolved analysis of the amplitude and phase of THz pulses propagating through three-dimensional photonic crystals is presented. Single-cycle pulses of THz radiation allow measurements over a wide frequency range, spanning more than an octave below, at and above the bandgap of strongly dispersive photonic crystals. Transmission data provide evidence for slow group velocities at the photonic band edges and for superluminal transmission at frequencies in the gap. Our experimental results are in good agreement with finite-difference-time-domain simulations.Comment: 7 pages, 11 figure

Time-resolved broadband analysis of slow-light propagation and superluminal transmission of electromagnetic waves in three-dimensional photonic crystals

A time-resolved analysis of the amplitude and phase of THz pulses propagating through three-dimensional photonic crystals is presented. Single-cycle pulses of THz radiation allow measurements over a wide frequency range, spanning more than an octave below, at and above the bandgap of strongly dispersive photonic crystals. Transmission data provide evidence for slow group velocities at the photonic band edges and for superluminal transmission at frequencies in the gap. Our experimental results are in good agreement with finite-difference-time-domain simulations