The V5A13.1 envelope glycoprotein deletion mutant of mouse hepatitis virus type-4 is neuroattenuated by its reduced rate of spread in the central nervous system.

Following intracerebral inoculation of adult Balb/c Byj mice, the MHV-4 strain of mouse hepatitis virus (MHV) had an LD50 of less than 0.1 PFU, whereas its monoclonal antibody resistant variant V5A13.1 had an LD50 of 10(4.2) PFU. To determine the basis for this difference in neurovirulence we have studied the acute central nervous system (CNS) infection of these two viruses by in situ hybridization. Both viruses infected the same, specific neuroanatomical areas, predominantly neurons, and spread via the cerebrospinal fluid, along neuronal pathways and between adjacent cells. The neuronal nuclei infected and the spread of virus within the brain are described. The main difference between the parental and variant viruses was the rate at which the infection spread. MHV-4 spread rapidly, destroying large numbers of neurons and the animals died within 4 days of infection. The variant virus spread to the same areas of the brain but at a slower rate. This difference in the rate of virus spread was also apparent from the brain virus titers. The slower rate of spread of the variant virus appears to allow intervention by the immune response. Consistent with this, the variant virus spread slowly in athymic nu/nu mice, but in the absence of an intact immune response, infection and destruction of neurons eventually reached the same extent as that of the parental virus and the mice died within 6 days of infection. We conclude that the V5A13.1 variant of MHV-4 is neuroattenuated by its slower rate of spread in the CNS

Thin electron-scale layers at the magnetopause

We use data from the four Cluster satellites to examine the microphysics of a thin electron-scale layer discovered on the magnetospheric side of the magnetopause. Here the ion and electron motions are decoupled in a layer about 20 km (a few electron scales) wide, including currents and strong electric fields. In this layer the electrons are E x B drifting with the ions as a background, and the region can be described by Hall MHD physics. A unique identification of the source of the thin layer is not possible, but our observations are consistent with recent simulations showing thin layers associated with the separatrix extending far away from a reconnection diffusion region

Cluster electron observations of the separatrix layer during traveling compression regions

[ 1] We present Cluster 4-point observations of electrons during traveling compression regions ( TCRs) on 19 September 2001. The electron and \B\ signatures vary with distance from the plasma sheet, confirming that transient plasma sheet bulges propagate past Cluster. TCRs with \B\ increases have either no electron signature, or unidirectional similar to1 keV electrons at the plasma sheet edge. However, spacecraft initially near the plasma sheet edge are engulfed within the bulge and observe a diamagnetic reduction in \B\. In cases where the underlying plasma sheet bulge moves earthward, electrons at the plasma sheet edge stream tailward. We suggest this represents either a remote observation of electrons closing the Hall current system in an ion diffusion region located farther tailward, or the outflow jets along the separatrix formed by a second neutral line located farther earthward of the spacecraft. The latter case implies the simultaneous action of multiple X-lines in the near-Earth tail

Electron Dynamics in the Diffusion Region of an Asymmetric Magnetic Reconnection

During a magnetopause crossing near the subsolar point Cluster observes the ion diffusion region of antiparallel magnetic reconnection. The reconnecting plasmas are asymmetric, differing in magnetic field strength, density, and temperature. Spatial changes in the electron distributions in the diffusion region are resolved and investigated in detail. Heating of magnetosheath electrons parallel to the magnetic field is observed. This heating is shown to be consistent with trapping of magnetosheath electrons by parallel electric fields

Cluster PEACE observations of electron pressure tensor divergence in the magnetotail

Cluster crossed the magnetotail neutral sheet on four occasions between 16: 38 and 16: 43 UT on 08/17/2003. The four-spacecraft capabilities of Cluster are used to determine spatial gradients from the magnetic field vectors and, for the first time, full electron pressure tensors. We find that the contribution to the electric field from the Hall term (max of similar to 6 mV/m) pointed towards the neutral sheet, whereas that from the electron pressure divergence ( max of similar to 1 mV/m) pointed away from the neutral sheet. The electric field contributions in this direction were closely anti-correlated. During this period Clusters 1 and 4 were sometimes above and below the neutral sheet respectively. This allowed the simultaneous observation of magnetic fields that are interpreted as two quadrants of the Hall magnetic field system. An associated field-aligned current system was detected using the curlometer and moments of the particle distributions

Fresh Thinking 2013-2050

The purpose of this report is to: • provide information on current production of a key group of fruit and vegetables grown in Western Australia and the amounts consumed, some of which are imported; • estimate how much of these fresh fruit and vegetables will be required to meet WA’s demand in 2025 and 2050; • estimate the water and land resource required to grow these fruit and vegetables under current production systems and project the demand for these resources to 2025 and 2050; and • consider the competitiveness of various fruit and vegetable industries and their capacity to meet emerging food demand locally, domestically and worldwide

Activation of PKR by Bunyamwera virus is independent of the viral interferon antagonist NSs

Double-stranded RNA (dsRNA) is a by-product of viral RNA polymerase activity, and its recognition is one mechanism by which the innate immune system is activated. Cellular responses to dsRNA include induction of alpha/beta interferon (IFN) synthesis and activation of the enzyme PKR, which exerts its antiviral effect by phosphorylating the eukaryotic initiation factor eIF-2 alpha, thereby inhibiting translation. We have recently identified the nonstructural protein NSs of Bunyamwera virus (BUNV), the prototype of the family Bunyaviridae, as a virulence factor that blocks the induction of IFN by dsRNA. Here, we investigated the potential of NSs to inhibit PKR. We show that wild-type (wt) BUNV that expresses NSs triggered PKR-dependent phosphorylation of eIF-2 alpha to levels similar to those of a recombinant virus that does not express NSs (BUNdelNSs virus). Furthermore, the sensitivity of viruses in cell culture to IFN was independent of PKR and was not determined by NSs. PKR knockout mice, however, succumbed to infection approximately 1 day earlier than wt mice or mice deficient in expression of RNase L, another dsRNA-activated antiviral enzyme. Our data indicate that (i) bunyaviruses activate PKR, but are only marginally sensitive to its antiviral effect, and (ii) NSs is different from other IFN antagonists, since it inhibits dsRNA-dependent IFN induction but has no effect on the dsRNA-activated PKR and RNase L systems