Incidence and predisposing factors of cognitive disorders following off-pump coronary artery bypasses graft surgery

Patients and Methods: A total of 171 patients who had undergone off-pump CABG without any history of psychiatric disorders were enrolled. Samples were selected according to a purposive sampling method. The Mini-Mental State Examination (MMSE) questionnaire was given to each patients to assess the incidence of cognitive disorder during the first 24 hours of surgery in ICU. To compare creatinine, erythrocytes sedimentation rate, extubation time, and patients’ age between those with and those without postoperative cognitive disorder, independent-samplest test was employed. To compare two groups in terms of any history of diseases such as diabetes, hypertension, and hyperlipidemia, and qualitative C-reactive protein (CRP), Chi square test was used. Results: Results showed that 75% of patients had postoperative cognitive disorder. There was a significant association between the history of hypertension, CRP, and preoperative creatinine levels in both cognitive disorder and control groups. Background: Cognitive disorder, which is a common problem for the hospitalized patients, is a fluctuating cognitive destruction that leads to the loss of consciousness. It is usually accompanied by increased mortality, prolonged hospital stay, and decreased rehabilitation. Objectives: The purpose of this study was to determine the risk factors associated with cognitive disorder after coronary artery bypasses graft surgery (CABG). Conclusions: Given the significant prevalence of postoperative cognitive disorder and significant association between the history of hypertension, CRP, and preoperative creatinine and cognitive disorder, the detection of patient’s clinical symptoms may improve diagnosis, treatment, and even prevention of cognitive disorder. © 2014, Iranian Society of Regional Anesthesia and Pain Medicine (ISRAPM); Published by Kowsar

Insights into Exosome Transport through the Blood–Brain Barrier and the Potential Therapeutical Applications in Brain Diseases

Drug delivery to the central nervous system (CNS) is limited due to the presence of the blood–brain barrier (BBB), a selective physiological barrier located at the brain microvessels that regulates the flow of cells, molecules and ions between the blood and the brain. Exosomes are nanosized extracellular vesicles expressed by all cell types and that function as cargos, allowing for communication between the cells. The exosomes were shown to cross or regulate the BBB in healthy and disease conditions. However, the mechanistic pathways by which exosomes cross the BBB have not been fully elucidated yet. In this review, we explore the transport mechanisms of exosomes through the BBB. A large body of evidence suggests that exosome transport through the BBB occurs primarily through transcytosis. The transcytosis mechanisms are influenced by several regulators. Inflammation and metastasis also enhance exosome trafficking across the BBB. We also shed light on the therapeutical applications of exosomes for treating brain diseases. Further investigations are essential to provide clearer insights related to trafficking of exosomes across the BBB and disease treatment

Targeting delivery of lipocalin 2-engineered mesenchymal stem cells to colon cancer in order to inhibit liver metastasis in nude mice

One of the major obstacles in cancer therapy is the lack of anticancer agent specificity to tumor tissues. The strategy of cell-based therapy is a promising therapeutic option for cancer treatment. The specific tumor-oriented migration of mesenchymal stem cells (MSCs) makes them a useful vehicle to deliver anticancer agents. In this study, we genetically manipulated bone marrow-derived mesenchymal stem cells with their lipocalin 2 (Lcn2) in order to inhibit liver metastasis of colon cancer in nude mice. Lcn2 was successfully overexpressed in transfected MSCs. The PCR results of SRY gene confirmed the presence of MSCs in cancer liver tissue. This study showed that Lcn2-engineered MSCs (MSC-Lcn2) not only inhibited liver metastasis of colon cancer but also downregulated the expression of vascular endothelial growth factor (VEGF) in the liver. Overall, MSCs by innate tropism toward cancer cells can deliver the therapeutic agent, Lcn2, and inhibit cancer metastasis. Hence, it could be a new modality for efficient targeted delivery of anticancer agent to liver metastasis. © 2015, International Society of Oncology and BioMarkers (ISOBM)

Examination of produced water from the Al-Hamada oilfield, Libya.

Produced water is generated to the surface from oil production. Because of the complex composition of produced water (a mixture of different organic and inorganic compounds and residues of oilfield chemicals - added to aid oil water separation) and due to the outcome of increasing volume and effect of discharging, its analysis has become a significant issue of environmental concern. For this purpose and also because of concerns over health and its safety, the chemical compositions of four produced water samples from Al-Hamada oilfield in the Libyan Arab desert were investigated in details. The physical-chemical properties included pH,TDS, EC, COD, cations and anions, organic compounds TOG, TPH, Base/Neutrals acids, Total phenols, BTEX, PAH carbon distribution of the oil extracted from the samples, inorganic compounds (heavy metals), added chemicals (corrosion inhibitor and biocides). Determinations were carried out using techniques, such as, GC-FID (HS-SPME), GC-MS, (ESI-MS/MS) and (LC-ESI-MS). The results indicate that the metals were within the expected natural ranges compared to those mentioned in the literature. Only manganese values were found to be higher than those in the literature in a range between 0.06-0.23ppm. BTEX and phenol were within range, 0.1- 0.2 and compounds acenaphthene, indeno(1,2,3-cd)pyrene, benzo(b)fluoranthene of PAH were identified in the range between0.4-2ppm. These originate could be from biodegradation by existing bacteria, which generally changes the chemical composition and reduce the toxicity of the water. Typical corrosion inhibitor chemicals quaternary ammonium compounds (quats) were detected with alkyl chain lengths of C12 (m/z 304), C14 (m/z 332), C16 (m/z 360) and C18 (m/z 388). On the whole, knowledge is needed about the level of oilfield chemicals in the produced water and groundwater and also phenol and alkyl phenols compound present that contribute to the environmental impact of produced water need to identify by GC-MS. Produced water should not be consumed by humans and animals

Developments in Rare Kaon Decay Physics

We review the current status of the field of rare kaon decays. The study of rare kaon decays has played a key role in the development of the standard model, and the field continues to have significant impact. The two areas of greatest import are the search for physics beyond the standard model and the determination of fundamental standard-model parameters. Due to the exquisite sensitivity of rare kaon decay experiments, searches for new physics can probe very high mass scales. Studies of the k->pnn modes in particular, where the first event has recently been seen, will permit tests of the standard-model picture of quark mixing and CP violation.Comment: One major revision to the text is the branching ratio of KL->ppg, based on a new result from KTeV. Several references were updated, with minor modifications to the text. A total of 48 pages, with 28 figures, in LaTeX; to be published in the Annual Review of Nuclear and Particle Science, Vol. 50, December 200

miR-27a-3p regulates expression of intercellular junctions at the brain endothelium and controls the endothelial barrier permeability

Background The brain endothelial barrier permeability is governed by tight and adherens junction protein complexes that restrict paracellular permeability at the blood-brain barrier (BBB). Dysfunction of the inter-endothelial junctions has been implicated in neurological disorders such as multiple sclerosis, stroke and Alzheimer’s disease. The molecular mechanisms underlying junctional dysfunction during BBB impairment remain elusive. MicroRNAs (miRNAs) have emerged as versatile regulators of the BBB function under physiological and pathological conditions, and altered levels of BBB-associated microRNAs were demonstrated in a number of brain pathologies including neurodegeneration and neuroinflammatory diseases. Among the altered micro-RNAs, miR-27a-3p was found to be downregulated in a number of neurological diseases characterized by loss of inter-endothelial junctions and disruption of the barrier integrity. However, the relationship between miR-27a-3p and tight and adherens junctions at the brain endothelium remains unexplored. Whether miR-27a-3p is involved in regulation of the junctions at the brain endothelium remains to be determined. Methods Using a gain-and-loss of function approach, we modulated levels of miR-27a-3p in an in-vitro model of the brain endothelium, key component of the BBB, and examined the resultant effect on the barrier paracellular permeability and on the expression of essential tight and adherens junctions. The mechanisms governing the regulation of junctional proteins by miR-27a-3p were also explored. Results Our results showed that miR-27a-3p inhibitor increases the barrier permeability and causes reduction of claudin-5 and occludin, two proteins highly enriched at the tight junction, while miR-27a-3p mimic reduced the paracellular leakage and increased claudin-5 and occludin protein levels. Interestingly, we found that miR-27-3p induces expression of claudin-5 and occludin by downregulating Glycogen Synthase Kinase 3 beta (GSK3ß) and activating Wnt/ ß-catenin signaling, a key pathway required for the BBB maintenance. Conclusion For the first time, we showed that miR-27a-3p is a positive regulator of key tight junction proteins, claudin-5 and occludin, at the brain endothelium through targeting GSK3ß gene and activating Wnt/ß-catenin signaling. Thus, miR-27a-3p may constitute a novel therapeutic target that could be exploited to prevent BBB dysfunction and preserves its integrity in neurological disorders characterized by impairment of the barrier’s function

Kaon decays and the flavour problem

After a brief introduction to the so-called flavour problem, we discuss the role of rare K decays in probing the mechanism of quark-flavour mixing. Particular attention is devoted to the formulation of the Minimal Flavour Violation hypothesis, as a general and natural solution to the flavour problem, and to the fundamental role of K -> pi nu nu-bar decays in testing this scenario.Comment: 10 pages, 6 figures, contribution to TH 2002 (Paris, July 2002

Effective Hamiltonian Approach to Hyperon Beta Decay with Final-State Baryon Polarization

Using an effective Hamiltonian approach, we obtain expressions for hyperon beta decay final-state baryon polarization. Terms through second order in the energy release are retained. The resulting approximate expressions are much simpler and more compact than the exact expressions, and they agree closely with them.Comment: 1 Figure Will appear in Phys Rev D 60 Article 117505 (Dec 1, 1999

General analysis of time evolution of decay spectrum in B^0 -> pi^+ pi^- l^+ l^-

Using the general, model independent form of effective Hamiltonian, the decay spectrum of B^0 -> pi^+ pi^- l^+ l^- decay is studied. The sensitivity of experimentally measurable asymmetries to the new Wilson coefficients and time is studied. It is observed that different asymmetries are sensitive to the new Wilson coefficients and they can serve as an efficient tool for establishing new physics beyond SM.Comment: 15 pp, 4 figures (postscript formatted), LaTex formatte

Experimental search for the decay mode K_L -> pi^0 gamma e^+ e^-

We report on results of a search for the decay mode K_L -> pi^0 gamma e^+ e^- conducted by the E162 experiment at KEK. We observed no events and set a 90% confidence level upper limit of Br(K_L -> pi^0 gamma e^+ e^-)< 7.1x10^{-7} for its branching ratio. This is the first published experimental result on this decay mode.Comment: 10 pages, 4 figures, submitted to Physics Letters