How, where and when to screen for porcine cytomegalovirus (PCMV) in donor pigs for xenotransplantation

Porcine cytomegalovirus (PCMV), that is actually a porcine roseolovirus (PRV), is a common herpesvirus in domestic pigs and wild boars. In xenotransplantation, PCMV/PRV has been shown to significantly reduce the survival time of pig kidneys and hearts in preclinical trials with different non-human primates. Furthermore, PCMV/PRV has been transmitted in the first pig to human heart xenotransplantation and contributed to the death of the patient. Although transmitted to the recipient, there is no evidence that PCMV/PRV can infect primate cells including human cells. PCMV/PRV is closely related to the human herpesviruses 6 and 7, and only distantly related to the human CMV (HCMV). Antiviral drugs used for the treatment of HCMV are less effective against PCMV/PRV. However, there are well described strategies to eliminate the virus from pig facilities. In order to detect the virus and to eliminate it, highly sensitive detection methods and the knowledge of how, where and when to screen the donor pigs is required. Here, a comparative testing of organs from pigs of different ages using polymerase chain reaction (PCR)-based and immunological methods was performed. Testing young piglets, PCMV/PRV was detected effectively by PCR in blood, bronchoalveolar lavage fluid, tonsils and heart. In adult animals, detection by PCR was not successful in most cases, because the virus load was below the detection limit or the virus was in its latent stage. Therefore, detection of antibodies against selected recombinant proteins corresponding to epitopes detected by nearly all infected animals in a Western blot assay is advantageous. By contrast, immunological testing is not beneficial in young animals as piglets might have PCMV/PRV-specific antibodies obtained from their infected mother via the colostrum. Using a thoughtful combination of PCR-based and immunological methods, detection of PCMV/PRV in donor pigs for xenotransplantation is feasible and a controlled elimination of the virus by early weaning or other methods is possible

A small unconditional non-financial incentive suggests an increase in survey response rates amongst older general practitioners (GPs): a randomised controlled trial study

BACKGROUND: Few studies have investigated the effect of small unconditional non-monetary incentives on survey response rates amongst GPs or medical practitioners. This study assessed the effectiveness of offering a small unconditional non-financial incentive to increase survey response rates amongst general practitioners within a randomised controlled trial (RCT). METHODS: An RCT was conducted within a general practice survey that investigated how to prolong working lives amongst ageing GPs in Australia. GPs (n = 125) were randomised to receive an attractive pen or no pen during their first invitation for participation in a survey. GPs could elect to complete the survey online or via mail. Two follow up reminders were sent without a pen to both groups. The main outcome measure was response rates. RESULTS: The response rate for GPs who received a pen was higher in the intervention group (61.9%) compared to the control group (46.8%). This study did not find a statistically significant effect of a small unconditional non-financial incentive (in the form of a pen) on survey response rates amongst GPs (Odds ratio, 95% confidence interval: 1.85 (0.91 to 3.77). No GPs completed the online version. CONCLUSION: A small unconditional non-financial incentives, in the form of a pen, may improve response rates for GPs

Can disability predict treatment outcome among traumatized refugees?

The aim of the present study was to examine the influence of disability on changes in symptoms of PTSD, anxiety, and depression among treatment-seeking traumatized refugees. Eighty-one refugees participated in different rehabilitation programs. PTSD symptomatology was assessed by the HTQ-IV and symptoms of depression and anxiety were assessed by the HSCL-25. Disability was assessed by the WHODAS 2.0 before treatment. Following treatment, no statistically significant changes in PTSD, depression, and anxiety symptom scores were observed. Disability in the domain ‘getting along’ was a significant predictor of PTSD scores following treatment, when controlling for baseline scores. Neither total disability nor individual disability domains predicted any other symptom changes. Living with one’s partner did, however, seem to be a consistent and significant predictor of treatment outcome. The results are discussed in terms of clinical implications and future research needs

Validation of a modified rat model for erectile function evaluation

The in vivo model for evaluation of erectile function in rats and mice has been widely used to investigate pathophysiology and treatment modalities of erectile function. The model is technically challenging which limits its broad availability. We have recently introduced a simplified surgical technique for dissection of corporal bodies and developed a new method to achieve stable contact between the cavernous nerve and the stimulating electrode without the need to manipulate the nerve between stimulations using 2-component silicone glue. The goal of this study was to validate this new technique and describe in detail the technical aspects of the procedure so that researchers with basic microsurgery skills can adopt it

Undergraduate Student Attitudes and Perspectives of the Accessibility, Supportiveness, and Appreciation of Research Opportunities in the Health Sciences

Undergraduate research is a “high-impact” educational practice that enriches student learning and facilitates student career advancement. This sequential explanatory mixed methods study, composed of a quantitative online questionnaire followed by qualitative focus group interviews, sought to explore undergraduate student attitudes on research and elicit perceived facilitators and barriers to undergraduate research engagement. The survey respondents (N = 377), all undergraduate health sciences students at McMaster University in Hamilton, Ontario, generally had positive attitudes toward undergraduate research, but had polarized perceptions of its accessibility, supportiveness, and appreciation. Follow-up focus group interviews with selected participants (N = 11) revealed four main themes: (1) the hidden curriculum of undergraduate research, (2) the paucity of meaningful research work for emerging student researchers, (3) the administrative barriers within the undergraduate research landscape, and (4) the inequitable access to undergraduate research opportunities. This study’s findings suggest potential avenues to improve the undergraduate student research experience,La recherche au premier cycle enrichit l’apprentissage des étudiants et facilite l’avancement de leur carrière. Cette étude séquenti-elle explicative à méthodes mixtes, comprenant un questionnaire suivi de groupes de discussion, visait à explorer les attitudes des étudiants de premier cycle en sciences de la santé à l’égard de la recherche, ainsi que les facilitateurs et les obstacles perçus quant à leur engagement dans celle-ci. Les n = 377 répondants au questionnaire avaient généralement une attitude positive à l’égard de la recherche au premier cycle, mais avaient des perceptions polarisées quant à son accessibilité, au soutien reçu et à l’appréciation obtenue. Les groupes de discussion avec n = 11 participants ont révélé quatre thèmes principaux : 1) le « programme caché » de la recherche au premier cycle; 2) la rareté des travaux de recherche significatifs pour les étudiants chercheurs; 3) les barrières adminis-tratives; et 4) l’accès inéquitable aux possibilités de recherche. Les résultats de cette étude suggèrent des méthodes pour améliorer l’expérience de recherche des étudiants de premier cycle