8,974 research outputs found
ELECTRON MICROSCOPIC AUTORADIOGRAPHY OF GERMINAL CENTER CELLS IN MOUSE SPLEEN
The fine structure of tritiated thymidine-labeled cells in antigen-stimulated mouse spleen germinal centers is described. In studies on the ultrastructural level, two labeled cell types found in germinal centers are observed. Large lymphocytes are characterized by their very numerous free ribosomes, a paucity of endoplasmic reticulum, relatively few mitochondria, and a poorly developed Golgi region. The nuclei are large and vesicular, and large nucleoli are present. A second labeled cell type appears to contain more mitochondria and has a higher development of the Golgi area. The nucleus contains large, numerous blocks of chromatin, indicative of a more differentiated cell type. Reticular cells, both phagocytic and non-phagocytic, were not observed to be labeled in the germinal centers
Effects of Force Level and Hand Dominance on Bilateral Transfer of a Fine Motor Skill
Our research is about bilateral transfer, a concept in motor learning where skills
learned by one limb are "transferred", allowing the opposite limb to benefit from what
was learned by the first limb. Previous research into bilateral transfer has raised
questions about whether specific aspects of motor coordination are or are not
transferred. We wanted to see whether learning to control pinch force by the thumb
and index finger is transferable, and if it is, whether the learning transfers equally
from either hand. We also want to look into the effects of different force levels on
the degree of transfer. We designed a task using a program that takes force levels
as inputs and has the participant trace shapes on a screen. By having participants
perform with one hand, then practice with the other, and finally perform again with
the initial hand, we can measure transfer as the difference in performance before
and after practice with the other hand.Kinesiology and Health Educatio
The Effects of Self-Myofascial Release vs. Instrument Assisted Soft Tissue Mobilization on Performance
Please download pdf version here
Correlation of chlorophyll, suspended matter, and related parameters of waters in the lower Chesapeake Bay area to LANDSAT-1 imagery
The author has identified the following significant results. An effort to relate water parameters of the lower Chesapeake Bay area to multispectral scanner images of LANDSAT 1 has shown that some spectral bands can be correlated to water parameters, and has demonstrated the feasibility of synoptic mapping of estuaries by satellite. Bands 5 and 6 were shown to be useful for monitoring total particles. Band 5 showed high correlation with suspended sediment concentration. Attenuation coefficients monitored continuously by ship along three baselines were cross correlated with radiance values on three days. Improved correlations resulted when tidal conditions were taken into consideration. A contouring program was developed to display sediment variation in the lower Chesapeake Bay from the MSS bands
Cryogenic Propulsion Stage (CPS) Configuration in Support of NASA's Multiple Design Reference Missions (DRMs)
In support of the National Aeronautics and Space Administration's (NASA) Human Exploration and Operations Mission Directorate (HEOMD), the Space Launch System (SLS) is being designed for safe, affordable, and sustainable human and scientific exploration missions beyond Earth's or-bit (BEO). The SLS Team is tasked with developing a system capable of safely and repeatedly lofting a new fleet of spaceflight vehicles beyond Earth orbit. The Cryogenic Propulsion Stage (CPS) is a key enabler for evolving the SLS capability for BEO missions. This paper reports on the methodology and initial recommendations relative to the CPS, giving a brief retrospective of early studies on this promising propulsion hardware. This paper provides an overview of the requirements development and CPS configuration in support of NASA's multiple Design Reference Missions (DRMs)
Optically trapped atom interferometry using the clock transition of large Rb-87 Bose-Einstein condensates
We present a Ramsey-type atom interferometer operating with an optically
trapped sample of 10^6 Bose-condensed Rb-87 atoms. The optical trap allows us
to couple the |F =1, mF =0>\rightarrow |F =2, mF =0> clock states using a
single photon 6.8GHz microwave transition, while state selective readout is
achieved with absorption imaging. Interference fringes with contrast
approaching 100% are observed for short evolution times. We analyse the process
of absorption imaging and show that it is possible to observe atom number
variance directly, with a signal-to-noise ratio ten times better than the
atomic projection noise limit on 10^6 condensate atoms. We discuss the
technical and fundamental noise sources that limit our current system, and
outline the improvements that can be made. Our results indicate that, with
further experimental refinements, it will be possible to produce and measure
the output of a sub-shot-noise limited, large atom number BEC-based
interferometer.
In an addendum to the original paper, we attribute our inability to observe
quantum projection noise to the stability of our microwave oscillator and
background magnetic field. Numerical simulations of the Gross-Pitaevskii
equations for our system show that dephasing due to spatial dynamics driven by
interparticle interactions account for much of the observed decay in fringe
visibility at long interrogation times. The simulations show good agreement
with the experimental data when additional technical decoherence is accounted
for, and suggest that the clock states are indeed immiscible. With smaller
samples of 5 \times 10^4 atoms, we observe a coherence time of {\tau} =
(1.0+0.5-0.3) s.Comment: 22 pages, 6 figures Addendum: 11 pages, 6 figure
Feshbach resonances in the 6Li-40K Fermi-Fermi mixture: Elastic versus inelastic interactions
We present a detailed theoretical and experimental study of Feshbach
resonances in the 6Li-40K mixture. Particular attention is given to the
inelastic scattering properties, which have not been considered before. As an
important example, we thoroughly investigate both elastic and inelastic
scattering properties of a resonance that occurs near 155 G. Our theoretical
predictions based on a coupled channels calculation are found in excellent
agreement with the experimental results. We also present theoretical results on
the molecular state that underlies the 155G resonance, in particular concerning
its lifetime against spontaneous dissociation. We then present a survey of
resonances in the system, fully characterizing the corresponding elastic and
inelastic scattering properties. This provides the essential information to
identify optimum resonances for applications relying on interaction control in
this Fermi-Fermi mixture.Comment: Submitted to EPJD, EuroQUAM special issues "Cold Quantum Matter -
Achievements and Prospects", v2 with updated calibration of magnetic field
(+4mG correction) and updated figures 4 and
Reverse Doppler effect in backward spin waves scattered on acoustic waves
We report on the observation of reverse Doppler effect in backward spin waves
reflected off of surface acoustic waves. The spin waves are excited in a
yttrium iron garnet (YIG) film. Simultaneously, acoustic waves are also
generated. The strain induced by the acoustic waves in the magnetostrictive YIG
film results in the periodic modulation of the magnetic anisotropy in the film.
Thus, in effect, a travelling Bragg grating for the spin waves is produced. The
backward spin waves reflecting off of this grating exhibit a reverse Doppler
shift: shifting down rather than up in frequency when reflecting off of an
approaching acoustic wave. Similarly, the spin waves are shifted up in
frequency when reflecting from receding acoustic waves.Comment: 4 pages, 3 figure
Shiga toxin 1 elicits diverse biologic responses in mesangial cells
Shiga toxin 1 elicits diverse biologic responses in mesangial cells.BackgroundShiga toxin 1 (Stx1) is a causative agent in hemolytic uremic syndrome (HUS). Its receptor, the glycosphingolipid globotriaosylceramide (Gb3), is expressed on cultured human endothelial and mesangial cells. Mesangial cell injury in HUS ranges from mild cellular edema to severe mesangiolysis and eventual glomerulosclerosis. We hypothesized that, in addition to endothelial cells, mesangial cells are targets of Stx1.MethodsHuman mesangial cells were exposed to Stx1. Protein synthesis was measured using [35S]-methionine/cysteine. Cell viability was measured as the lysosomal uptake of Neutral Red. Monocyte chemotactic peptide (MCP-1) mRNA and protein were analyzed by Northern blotting and ELISA.ResultsStx1 (0.25 to 2500ng/ml) resulted in a dose-dependent inhibition of protein synthesis. This effect of Stx1 was potentiated by preincubation of the cells with interleukin-1α (IL-1α; 2ng/ml) or tumor necrosis-α (TNF-α; 500 U/ml). Stx1 had little effect on mesangial cell viability during the first 24hours of exposure to Stx1. However, prolonged incubation with Stx1 for 48 and 72hours resulted in a 68% and 80% decrease in cell-viability, respectively. Stx1 elicited a dose and time dependent increase in the levels of MCP-1 mRNA, an effect that was potentiated by preincubation with IL-1α.ConclusionThese data indicate that mesangial cells are susceptible to the effects of Stx1 in vitro. Stx1 exerts a spectrum of biologic effects on mesangial cells ranging from activation of chemokine genes to a lethal toxic injury. Immunoinflammatory cytokines potentiate the effects of Stx1. Thus, glomerular pathology in HUS may also result from a direct effect of Stx1 on mesangial cells
- …