292 research outputs found
Waste Water Brine Purification through Electrodialysis Ion Exchange
Reutilizing resources onboard the International Space Station (ISS) and for future deep space missions are critical for mission longevity and sustainability. Waste water brine produced from water recovery systems contain chemical species that could be processed into a potential fertilizer for future plant systems.Fertilizer production can be achieved through a process called electrodialysis ion exchange. Waste water containing inorganic salt components are fed through a series of ion exchange membranes to produce fertilizer (a phosphate rich stream), electrolysis-grade water, and other useful commodities.A test bed was constructed to conduct controlled experiments and an experimental design procedure developed to determine the feasibility of the process. Conductivity and pH probes were utilized to determine the ion concentration in each of the product streams, along with ion chromatography (IC) to define the exact concentration of each ion in every stream throughout the experiment. This is crucial in order to convey the effectiveness of ion removal from the incoming waste water stream.The waste water and electrolyte streams were prepared in the lab prior to experimentation. Additionally, the ion exchange membrane configurations were developed and Opto 22 data analysis software incorporated to conduct measurements in real time.Ions successfully diffused across their respective membranes into the concentrate, acid, and base streams. This resulted in pure water, a phosphate rich stream, and a separate anion/hydrogen and cation/hydroxide stream
Presynaptic Type III Neuregulin1-ErbB signaling targets α7 nicotinic acetylcholine receptors to axons
Type III Neuregulin1 (Nrg1) isoforms are membrane-tethered proteins capable of participating in bidirectional juxtacrine signaling. Neuronal nicotinic acetylcholine receptors (nAChRs), which can modulate the release of a rich array of neurotransmitters, are differentially targeted to presynaptic sites. We demonstrate that Type III Nrg1 back signaling regulates the surface expression of α7 nAChRs along axons of sensory neurons. Stimulation of Type III Nrg1 back signaling induces an increase in axonal surface α7 nAChRs, which results from a redistribution of preexisting intracellular pools of α7 rather than from increased protein synthesis. We also demonstrate that Type III Nrg1 back signaling activates a phosphatidylinositol 3-kinase signaling pathway and that activation of this pathway is required for the insertion of preexisting α7 nAChRs into the axonal plasma membrane. These findings, in conjunction with prior results establishing that Type III Nrg1 back signaling controls gene transcription, demonstrate that Type III Nrg1 back signaling can regulate both short-and long-term changes in neuronal function
In Solidarity
This edition of Next Page is a departure from our usual question and answer format with a featured campus reader. Instead, we asked speakers who participated in the College’s recent Student Solidarity Rally (March 1, 2017) to recommend readings that might further our understanding of the topics on which they spoke
The Australia Telescope 20GHz (AT20G) Survey: analysis of the extragalactic source sample
The Australia Telescope 20 GHz (AT20G) survey is a blind survey of the whole
Southern sky at 20 GHz with follow-up observations at 4.8, 8.6, and 20 GHz
carried out with the Australia Telescope Compact Array (ATCA). In this paper we
present an analysis of radio spectral properties in total intensity and
polarisation, sizes, optical identifications, and redshifts of the sample of
the 5808 extragalactic sources in the survey catalogue of confirmed sources
over the whole Southern sky excluding the strip at Galactic latitude
|b|<1.5deg. The sample has a flux density limit of 40 mJy. Completeness has
been measured as a function of scan region and flux density. Averaging over the
whole survey area the follow-up survey is 78% complete above 50mJy and 93%
complete above 100mJy. 3332 sources with declination <-15deg have good quality
almost simultaneous observations at 4.8, 8.6, and 20GHz. The spectral analysis
shows that the sample is dominated by flat-spectrum sources. The fraction of
flat-spectrum sources decreases from 81% for 20GHz flux densities S>500mJy, to
60% for S<100mJy. There is also a clear spectral steepening at higher
frequencies with the median spectral index decreasing from -0.16 between 4.8
and 8.6GHz to -0.28 between 8.6 and 20GHz. Simultaneous observations in
polarisation are available for all the sources at all the frequencies. 768
sources have a good quality detection of polarised flux density at 20GHz; 467
of them were also detected in polarisation at 4.8 and/or at 8.6GHz so that it
has been possible to compare the spectral behaviour in total intensity and
polarisation. We have found that the polarised fraction increases slightly with
frequency and decreases with flux density. Cross matches and comparisons have
been made with other catalogues at lower radio frequencies, and in the optical,
X-ray and gamma-ray bands. Redshift estimates are available for 825 sources.Comment: 15 pages, 16 figures, accepted for publication in MNRA
Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting
We present an analytical method to quantify clustering in super-resolution
localization images of static surfaces in two dimensions. The method also
describes how over-counting of labeled molecules contributes to apparent
self-clustering and how the effective lateral resolution of an image can be
determined. This treatment applies to clustering of proteins and lipids in
membranes, where there is significant interest in using super-resolution
localization techniques to probe membrane heterogeneity. When images are
quantified using pair correlation functions, the magnitude of apparent
clustering due to over-counting will vary inversely with the surface density of
labeled molecules and does not depend on the number of times an average
molecule is counted. Over-counting does not yield apparent co-clustering in
double label experiments when pair cross-correlation functions are measured. We
apply our analytical method to quantify the distribution of the IgE receptor
(Fc{\epsilon}RI) on the plasma membranes of chemically fixed RBL-2H3 mast cells
from images acquired using stochastic optical reconstruction microscopy (STORM)
and scanning electron microscopy (SEM). We find that apparent clustering of
labeled IgE bound to Fc{\epsilon}RI detected with both methods arises from
over-counting of individual complexes. Thus our results indicate that these
receptors are randomly distributed within the resolution and sensitivity limits
of these experiments.Comment: 22 pages, 5 figure
'Reaching the hard to reach' - lessons learned from the VCS (voluntary and community Sector). A qualitative study.
Background The notion 'hard to reach' is a contested and ambiguous term that is commonly used within the spheres of social care and health, especially in discourse around health and social inequalities. There is a need to address health inequalities and to engage in services the marginalized and socially excluded sectors of society. Methods This paper describes a pilot study involving interviews with representatives from eight Voluntary and Community Sector (VCS) organisations . The purpose of the study was to explore the notion of 'hard to reach' and perceptions of the barriers and facilitators to accessing services for 'hard to reach' groups from a voluntary and community sector perspective. Results The 'hard to reach' may include drug users, people living with HIV, people from sexual minority communities, asylum seekers, refugees, people from black and ethnic minority communities, and homeless people although defining the notion of the 'hard to reach' is not straight forward. It may be that certain groups resist engaging in treatment services and are deemed hard to reach by a particular service or from a societal stance. There are a number of potential barriers for people who may try and access services, including people having bad experiences in the past; location and opening times of services and how services are funded and managed. A number of areas of commonality are found in terms of how access to services for 'hard to reach' individuals and groups could be improved including: respectful treatment of service users, establishing trust with service users, offering service flexibility, partnership working with other organisations and harnessing service user involvement.
Conclusions: If health services are to engage with groups that are deemed 'hard to reach' and marginalised from mainstream health services, the experiences and practices for engagement from within the VCS may serve as useful lessons for service improvement for statutory health services
Mass spectrometry-directed structure elucidation and total synthesis of ultra-long chain (O-acyl)-ω-hydroxy fatty acids
The (O-acyl)-ω-hydroxy FAs (OAHFAs) comprise an unusual lipid subclass present in the skin, vernix caseosa, and meibomian gland secretions. Although they are structurally related to the general class of FA esters of hydroxy FAs (FAHFAs), the ultra-long chain (30-34 carbons) and the putative -substitution of the backbone hydroxy FA suggest that OAHFAs have unique biochemistry. Complete structural elucidation of OAHFAs has been challenging because of their low abundance within complex lipid matrices. Furthermore, because these compounds occur as a mixture of closely related isomers, insufficient spectroscopic data have been obtained to guide structure confirmation by total synthesis. Here, we describe the full molecular structure of ultra-long chain OAHFAs extracted from human meibum by exploiting the gas-phase purification of lipids through multistage MS and novel multidimensional ion activation methods. The analysis elucidated sites of unsaturation, the stereochemical configuration of carbon-carbon double bonds, and ester linkage regiochemistry. Such isomer-resolved MS guided the first total synthesis of an ultra-long chain OAHFA, which, in turn, confirmed the structure of the most abundant OAHFA found in human meibum, OAHFA 50:2. The availability of a synthetic OAHFA opens new territory for future investigations into the unique biophysical and biochemical properties of these lipids
The Australia Telescope 20 GHz (AT20G) Survey: The Bright Source Sample
The Australia Telescope 20 GHz (AT20G) Survey is a blind survey of the whole
Southern sky at 20 GHz (with follow-up observations at 4.8 and 8.6 GHz) carried
out with the Australia Telescope Compact Array (ATCA) from 2004 to 2007. The
Bright Source Sample (BSS) is a complete flux-limited subsample of the AT20G
Survey catalogue comprising 320 extragalactic (|b|>1.5 deg) radio sources south
of dec = -15 deg with S(20 GHz) > 0.50 Jy. Of these, 218 have near simultaneous
observations at 8 and 5 GHz. In this paper we present an analysis of radio
spectral properties in total intensity and polarisation, size, optical
identifications and redshift distribution of the BSS sources. The analysis of
the spectral behaviour shows spectral curvature in most sources with spectral
steepening that increases at higher frequencies (the median spectral index
\alpha, assuming S\propto \nu^\alpha, decreases from \alpha_{4.8}^{8.6}=0.11
between 4.8 and 8.6 GHz to \alpha_{8.6}^{20}=-0.16 between 8.6 and 20 GHz),
even if the sample is dominated by flat spectra sources (85 per cent of the
sample has \alpha_{8.6}^{20}>-0.5). The almost simultaneous spectra in total
intensity and polarisation allowed us a comparison of the polarised and total
intensity spectra: polarised fraction slightly increases with frequency, but
the shapes of the spectra have little correlation. Optical identifications
provided an estimation of redshift for 186 sources with a median value of 1.20
and 0.13 respectively for QSO and galaxies.Comment: 34 pages, 19 figures, tables of data included, replaced with version
published in MNRA
Gut microbes shape microglia and cognitive function during malnutrition
Fecal-oral contamination promotes malnutrition pathology. Lasting consequences of early life malnutrition include cognitive impairment, but the underlying pathology and influence of gut microbes remain largely unknown. Here, we utilize an established murine model combining malnutrition and iterative exposure to fecal commensals (MAL-BG). The MAL-BG model was analyzed in comparison to malnourished (MAL mice) and healthy (CON mice) controls. Malnourished mice display poor spatial memory and learning plasticity, as well as altered microglia, non-neuronal CNS cells that regulate neuroimmune responses and brain plasticity. Chronic fecal-oral exposures shaped microglial morphology and transcriptional profile, promoting phagocytic features in MAL-BG mice. Unexpectedly, these changes occurred independently from significant cytokine-induced inflammation or blood-brain barrier (BBB) disruption, key gut-brain pathways. Metabolomic profiling of the MAL-BG cortex revealed altered polyunsaturated fatty acid (PUFA) profiles and systemic lipoxidative stress. In contrast, supplementation with an ω3 PUFA/antioxidant-associated diet (PAO) mitigated cognitive deficits within the MAL-BG model. These findings provide valued insight into the malnourished gut microbiota-brain axis, highlighting PUFA metabolism as a potential therapeutic target
The Australia Telescope 20 GHz Survey: The Source Catalogue
We present the full source catalogue from the Australia Telescope 20 GHz
(AT20G) Survey. The AT20G is a blind radio survey carried out at 20 GHz with
the Australia Telescope Compact Array (ATCA) from 2004 to 2008, and covers the
whole sky south of declination 0 deg. The AT20G source catalogue presented here
is an order of magnitude larger than any previous catalogue of high-frequency
radio sources, and includes 5890 sources above a 20 GHz flux-density limit of
40 mJy. All AT20G sources have total intensity and polarisation measured at 20
GHz, and most sources south of declination -15 deg also have near-simultaneous
flux-density measurements at 5 and 8 GHz. A total of 1559 sources were detected
in polarised total intensity at one or more of the three frequencies. We detect
a small but significant population of non-thermal sources that are either
undetected or have only weak detections in low-frequency catalogues. We
introduce the term Ultra-Inverted Spectrum (UIS) to describe these radio
sources, which have a spectral index alpha(5, 20) > +0.7 and which constitute
roughly 1.2 per cent of the AT20G sample. The 20 GHz flux densities measured
for the strongest AT20G sources are in excellent agreement with the WMAP 5-year
source catalogue of Wright et al. (2009), and we find that the WMAP source
catalogue is close to complete for sources stronger than 1.5 Jy at 23 GHz.Comment: 21 pages, accepted for publication in MNRA
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