The development and application of fecal indexes for predicting dry matter intake, dry matter digestibility and fecal dry matter output

Analytical analyses were conducted upon thirty-six fecal samples obtained from two digestion trials to determine Dry Matter, Nitrogen, Acid Detergent Fiber, Acid Detergent Lignin and Urobilinogen. In addition, individual in vivo Digestible Dry Matter was calculated from total fecal collection procedures. A technique was developed for quantitative urobilinogen analysis of dried fecal samples. From these independent variables cited, and the independent variable animal weight, equations were developed for predicting Fecal Dry Matter Output, Dry Matter Intake, Digestible Dry Matter, and Digestible Dry Matter Intake. A prediction equation was developed which accounted for over 43 percent of the variation in Fecal Dry Matter Output (P\u27\u3c 0.0003). The equation included the independent variable Urobilinogen. Acid Detergent Lignin, and Animal Weight. It was found that 78 percent of the variation in Dry Matter Intake (P \u3c 0.0001) could be explained by an equation containing the independent variables Nitrogen, Acid Detergent Lignin, and Urobilinogen Cubed. An equation including the independent variables Nitrogen, Acid Detergent Lignin, Acid Detergent Fiber, and the Natural Log of Urobilinogen explained 67 percent of the variation in Digestible Dry Matter (P \u3c 0.0001). Digestible Dry Matter Intake was found to be the most predictable of all the dependent variables studied. Almost 83 percent of the variation in Digestible Dry Matter Intake (P \u3c 0.0001) was accounted for by an equation containing the independent variables Nitrogen, Acid Detergent Fiber, and Urobilinogen Cubed. The large amount of explainable variation in the dependent variables considered in this study was achieved by using different regression equations for each dependent variable to be predicted. It was concluded, therefore, that no one fecal index could best explain the variation in all dependent variables of interest. Rather, several fecal indexes must be employed if more than one dependent variable is to be predicted. The value of fecal indexes in the evaluation of grazed forages is supported by the results of this study

Cortical oscillatory dynamics and benzodiazepine-site modulation of tonic inhibition in fast spiking interneurons

Tonic conductance mediated by extrasynaptic GABAA receptors has been implicated in the modulation of network oscillatory activity. Using an in vitro brain slice to produce oscillatory activity and a kinetic model of GABAA receptor dynamics, we show that changes in tonic inhibitory input to fast spiking interneurons underlie benzodiazepine-site mediated modulation of neuronal network synchrony in rat primary motor cortex. We found that low concentrations (10 nM) of the benzodiazepine site agonist, zolpidem, reduced the power of pharmacologically-induced beta-frequency (15-30 Hz) oscillatory activity. By contrast, higher doses augmented beta power. Application of the antagonist, flumazenil, also increased beta power suggesting endogenous modulation of the benzodiazepine binding site. Voltage-clamp experiments revealed that pharmacologically-induced rhythmic inhibitory postsynaptic currents were reduced by 10 nM zolpidem, suggesting an action on inhibitory interneurons. Further voltage-clamp studies of fast spiking cells showed that 10 nM zolpidem augmented a tonic inhibitory GABAA receptor mediated current in fast spiking cells whilst higher concentrations of zolpidem reduced the tonic current. A kinetic model of zolpidem-sensitive GABAA receptors suggested that incubation with 10 nM zolpidem resulted in a high proportion of GABAA receptors locked in a kinetically slow desensitized state whilst 30 nM zolpidem favoured rapid transition into and out of desensitized states. This was confirmed experimentally using a challenge with saturating concentrations of GABA. Selective modulation of an interneuron-specific tonic current may underlie the reversal of cognitive and motor deficits afforded by low-dose zolpidem in neuropathological states

Clustering around radio galaxies at z~1.5

The importance of studying old elliptical galaxies at redshift z ~ 1.5 is reviewed, considering both what can be learned by extending studies of the evolution of cluster galaxy scaling relations to earlier cosmic epochs, and the age-dating of old elliptical galaxies at high redshifts. Following this, the first results are provided of an on-going project to find such distant elliptical galaxies, through an investigation of the cluster environments of powerful radio sources with redshifts 1.44 < z < 1.7. These studies show a considerable excess of red galaxies in the radio sources fields, with the magnitudes (K >~ 17.5) and colours (R-K > 4) expected of old passively evolving galaxies at the radio source redshift. The red galaxy overdensities are found on two different scales around the radio sources; a pronounced small-scale peak at radial distances of <~ 150 kpc, and a weaker large-scale excess extending out to 1 - 1.5 Mpc. The presence and richness of these red galaxy excesses varies considerably from source to source. An interpretation of these results is provided.Comment: LaTeX, 6 pages, Elsevier Science format. To appear in "Radio galaxies: past, present & future". eds. M. Jarvis et al., Leiden, Nov 200

GABA-mediated changes in inter-hemispheric beta frequency activity in early-stage Parkinson's disease

In Parkinson's disease (PD), elevated beta (15-35Hz) power in subcortical motor networks is widely believed to promote aspects of PD symptomatology, moreover, a reduction in beta power and coherence accompanies symptomatic improvement following effective treatment with l-DOPA. Previous studies have reported symptomatic improvements that correlate with changes in cortical network activity following GABAA receptor modulation. In this study we have used whole-head magnetoencephalography to characterize neuronal network activity, at rest and during visually cued finger abductions, in unilaterally symptomatic PD and age-matched control participants. Recordings were then repeated following administration of sub-sedative doses of the hypnotic drug zolpidem (0.05mg/kg), which binds to the benzodiazepine site of the GABAA receptor. A beamforming based 'virtual electrode' approach was used to reconstruct oscillatory power in the primary motor cortex (M1), contralateral and ipsilateral to symptom presentation in PD patients or dominant hand in control participants. In PD patients, contralateral M1 showed significantly greater beta power than ipsilateral M1. Following zolpidem administration contralateral beta power was significantly reduced while ipsilateral beta power was significantly increased resulting in a hemispheric power ratio that approached parity. Furthermore, there was highly significant correlation between hemispheric beta power ratio and Unified Parkinson's Disease Rating Scale (UPDRS). The changes in contralateral and ipsilateral beta power were reflected in pre-movement beta desynchronization and the late post-movement beta rebound. However, the absolute level of movement-related beta desynchronization was not altered. These results show that low-dose zolpidem not only reduces contralateral beta but also increases ipsilateral beta, while rebalancing the dynamic range of M1 network oscillations between the two hemispheres. These changes appear to underlie the symptomatic improvements afforded by low-dose zolpidem

Near-Infrared Galaxy Counts to J and K ~ 24 as a Function of Image Size

We have used the Keck 10m telescope to count objects as a function of image size in 2 high galactic latitude fields covering 1.5 arcmin^2 and reaching 50% completeness at K=24 and J=24.5 for stellar sources. Counts extend ~1 mag deeper in K than surveys with other telescopes; complement Keck surveys providing counts at comparable or shallower depths but not utilizing image structure; and extend by several magnitudes the J band counts from other surveys. We find the surface-density of objects at K=23 to be higher than previously found (~500,000/mag/deg^2), but at K<22 to be consistent with most other surveys in amplitude and slope (~0.36). J band counts have similar slope. J and K counts are in excess of our empirical no-evolution models for an open universe, and a factor of 2 higher than mild-evolution models at J and K ~ 23. The slope of the model counts is insensitive to geometry even in the near-infrared because the counts are dominated by low-luminosity (<0.1L*) objects at modest redshift (z<1) with small apparent sizes (r05<0.4", i.e. <4 kpc). The observed counts rise most steeply for these smaller objects, which dominate fainter than K=22.3 and J=23.3. However, the greatest excess relative to no-evolution models occurs for the apparently larger objects (median J-K~1.5). The size and colors of such objects correspond equally well to luminous (>0.1L*), galaxies at 1<z<4, or progressively more diffuse, low-luminosity (0.001-0.1L*) galaxies at z<1. We rule out the excess is from very low luminosity (<0.0001L*) red galaxies at z<0.25. There is a deficit of galaxies with red J-K colors corresponding to luminous, early-type galaxies at 1<z<3. Assuming the deficit is due to their appearance as blue galaxies, they account only for 10-30% of the excess of large, blue galaxies. [abridged]Comment: accepted for publication in ApJ; 34 pages text, 9 tables, 10 figures (embedded); full resolution figures available at http://www.astro.wisc.edu/~mab/publications/pub.htm

Anatomy of a post-starburst minor merger: a multi-wavelength WFC3 study of NGC 4150

(Abridged) We present a spatially-resolved near-UV/optical study of NGC 4150, using the Wide Field Camera 3 (WFC3) on board the Hubble Space Telescope. Previous studies of this early-type galaxy (ETG) indicate that it has a large reservoir of molecular gas, exhibits a kinematically decoupled core (likely indication of recent merging) and strong, central H_B absorption (indicative of young stars). The core of NGC 4150 shows ubiquitous near-UV emission and remarkable dusty substructure. Our analysis shows this galaxy to lie in the near-UV green valley, and its pixel-by-pixel photometry exhibits a narrow range of near-UV/optical colours that are similar to those of nearby E+A (post-starburst) galaxies. We parametrise the properties of the recent star formation (age, mass fraction, metallicity and internal dust content) in the NGC 4150 pixels by comparing the observed near-UV/optical photometry to stellar models. The typical age of the recent star formation (RSF) is around 0.9 Gyrs, consistent with the similarity of the near-UV colours to post-starburst systems, while the morphological structure of the young component supports the proposed merger scenario. The RSF metallicity, representative of the metallicity of the gas fuelling star formation, is around 0.3 - 0.5 Zsun. Assuming that this galaxy is a merger and that the gas is sourced mainly from the infalling companion, these metallicities plausibly indicate the gas-phase metallicity (GPM) of the accreted satellite. Comparison to the local mass-GPM relation suggests (crudely) that the mass of the accreted system is around 3x10^8 Msun, making NGC 4150 a 1:20 minor merger. A summation of the pixel RSF mass fractions indicates that the RSF contributes about 2-3 percent of the stellar mass. This work reaffirms our hypothesis that minor mergers play a significant role in the evolution of ETGs at late epochs.Comment: 28 pages, 2 tables, accepted for publication in Ap

Review of the Palaearctic species of Ismaridae Thomson, 1858 (Hymenoptera: Diaprioidea)

This is an open access article, available to all readers online, published under a Creative Commons BY-NC-ND license: https://creativecommons.org/licenses/by-nc-nd/3.0/. The attached file is the published version of the article

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

BACKGROUND: Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp. psittaci outer membrane protein A (ompA) gene in pharyngeal swabs. METHODS: The fluorescein-biotin labelled PCR products were immobilized on streptavidin-coated microtiter plates and detected with anti-fluorescein peroxidase conjugate and a colorimetric substrate. An internal inhibition control was included to rule out the presence of inhibitors of DNA amplification. The diagnostic value of the ompA nested PCR-EIA in comparison to cell culture and a 16S-rRNA based nested PCR was assessed in pharyngeal turkey swabs from 10 different farms experiencing respiratory disease. RESULTS: The sensitivity of the nested PCR-EIA was established at 0.1 infection forming units (IFU). Specificity was 100%. The ompA nested PCR-EIA was more sensitive than the 16S-rRNA based nested PCR and isolation, revealing 105 out of 200 (52.5%) positives against 13 and 74 for the latter two tests, respectively. Twenty-nine (23.8%) out of 122 ompA PCR-EIA negatives showed the presence of inhibitors of DNA amplification, although 27 of them became positive after diluting (1/10) the specimens in PCR buffer or after phenol-chloroform extraction and subsequent ethanol precipitation. CONCLUSION: The present study stresses the need for an internal control to confirm PCR true-negatives and demonstrates the high prevalence of chlamydiosis in Belgian turkeys and its potential zoonotic risk. The ompA nested PCR-EIA described here is a rapid, highly sensitive and specific diagnostic assay and will help to facilitate the diagnosis of Cp. psittaci infections in both poultry and man

Dopamine acting at D1-like, D2-like and α1-adrenergic receptors differentially modulates theta and gamma oscillatory activity in primary motor cortex

The loss of dopamine (DA) in Parkinson’s is accompanied by the emergence of exaggerated theta and beta frequency neuronal oscillatory activity in the primary motor cortex (M1) and basal ganglia. DA replacement therapy or deep brain stimulation reduces the power of these oscillations and this is coincident with an improvement in motor performance implying a causal relationship. Here we provide in vitro evidence for the differential modulation of theta and gamma activity in M1 by DA acting at receptors exhibiting conventional and non-conventional DA pharmacology. Recording local field potentials in deep layer V of rat M1, co-application of carbachol (CCh, 5 μM) and kainic acid (KA, 150 nM) elicited simultaneous oscillations at a frequency of 6.49 ± 0.18 Hz (theta, n = 84) and 34.97 ± 0.39 Hz (gamma, n = 84). Bath application of DA resulted in a decrease in gamma power with no change in theta power. However, application of either the D1-like receptor agonist SKF38393 or the D2-like agonist quinpirole increased the power of both theta and gamma suggesting that the DA-mediated inhibition of oscillatory power is by action at other sites other than classical DA receptors. Application of amphetamine, which promotes endogenous amine neurotransmitter release, or the adrenergic α1-selective agonist phenylephrine mimicked the action of DA and reduced gamma power, a result unaffected by prior co-application of D1 and D2 receptor antagonists SCH23390 and sulpiride. Finally, application of the α1-adrenergic receptor antagonist prazosin blocked the action of DA on gamma power suggestive of interaction between α1 and DA receptors. These results show that DA mediates complex actions acting at dopamine D1-like and D2-like receptors, α1 adrenergic receptors and possibly DA/α1 heteromultimeric receptors to differentially modulate theta and gamma activity in M1

Spike firing and IPSPs in layer V pyramidal neurons during beta oscillations in rat primary motor cortex (M1) in vitro

Beta frequency oscillations (10-35 Hz) in motor regions of cerebral cortex play an important role in stabilising and suppressing unwanted movements, and become intensified during the pathological akinesia of Parkinson's Disease. We have used a cortical slice preparation of rat brain, combined with concurrent intracellular and field recordings from the primary motor cortex (M1), to explore the cellular basis of the persistent beta frequency (27-30 Hz) oscillations manifest in local field potentials (LFP) in layers II and V of M1 produced by continuous perfusion of kainic acid (100 nM) and carbachol (5 µM). Spontaneous depolarizing GABA-ergic IPSPs in layer V cells, intracellularly dialyzed with KCl and IEM1460 (to block glutamatergic EPSCs), were recorded at -80 mV. IPSPs showed a highly significant (P< 0.01) beta frequency component, which was highly significantly coherent with both the Layer II and V LFP oscillation (which were in antiphase to each other). Both IPSPs and the LFP beta oscillations were abolished by the GABAA antagonist bicuculline. Layer V cells at rest fired spontaneous action potentials at sub-beta frequencies (mean of 7.1+1.2 Hz; n = 27) which were phase-locked to the layer V LFP beta oscillation, preceding the peak of the LFP beta oscillation by some 20 ms. We propose that M1 beta oscillations, in common with other oscillations in other brain regions, can arise from synchronous hyperpolarization of pyramidal cells driven by synaptic inputs from a GABA-ergic interneuronal network (or networks) entrained by recurrent excitation derived from pyramidal cells. This mechanism plays an important role in both the physiology and pathophysiology of control of voluntary movement generation