A Test pf Calf Rations -- Methods of Controlling Contamination of Milk during Milking

A Test of Calf Rations Since the spring of 1899 the Department of Dairy Husbandry has been conducting experiments in calf rearing by using hand separator skim-milk and light rations of grain. The first test was published in Bulletin No. 68 of this Station and dealt with a comparison of calves reared by hand with those sucking their dams. The hand-fed calves were reared on skim-milk, ground flaxseed being used to replace the lacking butter fat. This test resulted in a fine lot of calves reared at a very low cost, but it also brought out the fact that much more data were needed in the way of comparing rations to be used with skim-milk. Methods of Controlling Contamination of Milk During Milking Milk contamination takes place largely during the process of milking, especially when the cows are kept in the barn. It was, therefore, thought best to start\u27 with investigations along this first and most troublesome source of infection, namely, milking. The plan of the experiment was to test a number of advocated methods of lessening milk contamination. \u27To do this, four cows were selected from the University dairy herd, all uniform in breed, weight, and time of lactation, so as to avoid, as much as possible,variation among individual animals

A Test pf Calf Rations -- Methods of Controlling Contamination of Milk during Milking

A Test of Calf Rations Since the spring of 1899 the Department of Dairy Husbandry has been conducting experiments in calf rearing by using hand separator skim-milk and light rations of grain. The first test was published in Bulletin No. 68 of this Station and dealt with a comparison of calves reared by hand with those sucking their dams. The hand-fed calves were reared on skim-milk, ground flaxseed being used to replace the lacking butter fat. This test resulted in a fine lot of calves reared at a very low cost, but it also brought out the fact that much more data were needed in the way of comparing rations to be used with skim-milk. Methods of Controlling Contamination of Milk During Milking Milk contamination takes place largely during the process of milking, especially when the cows are kept in the barn. It was, therefore, thought best to start\u27 with investigations along this first and most troublesome source of infection, namely, milking. The plan of the experiment was to test a number of advocated methods of lessening milk contamination. \u27To do this, four cows were selected from the University dairy herd, all uniform in breed, weight, and time of lactation, so as to avoid, as much as possible,variation among individual animals

Quantum Cryptography

Quantum cryptography could well be the first application of quantum mechanics at the individual quanta level. The very fast progress in both theory and experiments over the recent years are reviewed, with emphasis on open questions and technological issues.Comment: 55 pages, 32 figures; to appear in Reviews of Modern Physic

A Cis-Regulatory Map of the Drosophila Genome

Systematic annotation of gene regulatory elements is a major challenge in genome science. Direct mapping of chromatin modification marks and transcriptional factor binding sites genome-wide1, 2 has successfully identified specific subtypes of regulatory elements3. In Drosophila several pioneering studies have provided genome-wide identification of Polycomb response elements4, chromatin states5, transcription factor binding sites6, 7, 8, 9, RNA polymerase II regulation8 and insulator elements10; however, comprehensive annotation of the regulatory genome remains a significant challenge. Here we describe results from the modENCODE cis-regulatory annotation project. We produced a map of the Drosophila melanogaster regulatory genome on the basis of more than 300 chromatin immunoprecipitation data sets for eight chromatin features, five histone deacetylases and thirty-eight site-specific transcription factors at different stages of development. Using these data we inferred more than 20,000 candidate regulatory elements and validated a subset of predictions for promoters, enhancers and insulators in vivo. We identified also nearly 2,000 genomic regions of dense transcription factor binding associated with chromatin activity and accessibility. We discovered hundreds of new transcription factor co-binding relationships and defined a transcription factor network with over 800 potential regulatory relationships

A Rho Scaffold Integrates the Secretory System with Feedback Mechanisms in Regulation of Auxin Distribution

In plants, auxin distribution and tissue patterning are coordinated via a feedback loop involving the auxin-regulated cell polarity factor ICR1 and the secretory machinery

Transitional Care for Patients with Congenital Colorectal Diseases: An EUPSA Network Office, ERNICA, and eUROGEN Joint Venture

Background: Transition of care (TOC; from childhood into adulthood) of patients with anorectal malformations (ARM) and Hirschsprung disease (HD) ensures continuation of care for these patients. The aim of this international study was to assess the current status of TOC and adult care (AC) programs for patients with ARM and HD. Methods: A survey was developed by members of EUPSA, ERN eUROGEN, and ERNICA, including patient representatives (ePAGs), comprising of four domains: general information, general questions about transition to adulthood, and disease-specific questions regarding TOC and AC programs. Recruitment of centres was done by the ERNs and EUPSA, using mailing lists and social media accounts. Only descriptive statistics were reported. Results: In total, 82 centres from 21 different countries entered the survey. Approximately half of them were ERN network members. Seventy-two centres (87.8%) had a self-reported area of expertise for both ARM and HD. Specific TOC programs were installed in 44% of the centres and AC programs in 31% of these centres. When comparing centres, wide variation was observed in the content of the programs. Conclusion: Despite the awareness of the importance of TOC and AC programs, these programs were installed in less than 50% of the participating centres. Various transition and AC programs were applied, with considerable heterogeneity in implementation, content and responsible caregivers involved. Sharing best practice examples and taking into account local and National Health Care Programs might lead to a better continuation of care in the future. Level of Evidence: III

Systematic MicroRNA Analysis Identifies ATP6V0C as an Essential Host Factor for Human Cytomegalovirus Replication

Recent advances in microRNA target identification have greatly increased the number of putative targets of viral microRNAs. However, it is still unclear whether all targets identified are biologically relevant. Here, we use a combined approach of RISC immunoprecipitation and focused siRNA screening to identify targets of HCMV encoded human cytomegalovirus that play an important role in the biology of the virus. Using both a laboratory and clinical strain of human cytomegalovirus, we identify over 200 putative targets of human cytomegalovirus microRNAs following infection of fibroblast cells. By comparing RISC-IP profiles of miRNA knockout viruses, we have resolved specific interactions between human cytomegalovirus miRNAs and the top candidate target transcripts and validated regulation by western blot analysis and luciferase assay. Crucially we demonstrate that miRNA target genes play important roles in the biology of human cytomegalovirus as siRNA knockdown results in marked effects on virus replication. The most striking phenotype followed knockdown of the top target ATP6V0C, which is required for endosomal acidification. siRNA knockdown of ATP6V0C resulted in almost complete loss of infectious virus production, suggesting that an HCMV microRNA targets a crucial cellular factor required for virus replication. This study greatly increases the number of identified targets of human cytomegalovirus microRNAs and demonstrates the effective use of combined miRNA target identification and focused siRNA screening for identifying novel host virus interactions