Constitutive Activation of the Shaker Kv Channel

In different types of K+ channels the primary activation gate is thought to reside near the intracellular entrance to the ion conduction pore. In the Shaker Kv channel the gate is closed at negative membrane voltages, but can be opened with membrane depolarization. In a previous study of the S6 activation gate in Shaker (Hackos, D.H., T.H. Chang, and K.J. Swartz. 2002. J. Gen. Physiol. 119:521–532.), we found that mutation of Pro 475 to Asp results in a channel that displays a large macroscopic conductance at negative membrane voltages, with only small increases in conductance with membrane depolarization. In the present study we explore the mechanism underlying this constitutively conducting phenotype using both macroscopic and single-channel recordings, and probes that interact with the voltage sensors or the intracellular entrance to the ion conduction pore. Our results suggest that constitutive conduction results from a dramatic perturbation of the closed-open equilibrium, enabling opening of the activation gate without voltage-sensor activation. This mechanism is discussed in the context of allosteric models for activation of Kv channels and what is known about the structure of this critical region in K+ channels

Scanning the Intracellular S6 Activation Gate in the Shaker K+ Channel

In Kv channels, an activation gate is thought to be located near the intracellular entrance to the ion conduction pore. Although the COOH terminus of the S6 segment has been implicated in forming the gate structure, the residues positioned at the occluding part of the gate remain undetermined. We use a mutagenic scanning approach in the Shaker Kv channel, mutating each residue in the S6 gate region (T469-Y485) to alanine, tryptophan, and aspartate to identify positions that are insensitive to mutation and to find mutants that disrupt the gate. Most mutants open in a steeply voltage-dependent manner and close effectively at negative voltages, indicating that the gate structure can both support ion flux when open and prevent it when closed. We find several mutant channels where macroscopic ionic currents are either very small or undetectable, and one mutant that displays constitutive currents at negative voltages. Collective examination of the three types of substitutions support the notion that the intracellular portion of S6 forms an activation gate and identifies V478 and F481 as candidates for occlusion of the pore in the closed state

Observing Users - Designing clarity a case study on the user-centred design of a cross-language information retrieval system

This paper presents a case study of the development of an interface to a novel and complex form of document retrieval: searching for texts written in foreign languages based on native language queries. Although the underlying technology for achieving such a search is relatively well understood, the appropriate interface design is not. A study involving users (with such searching needs) from the start of the design process is described covering initial examination of user needs and tasks; preliminary design and testing of interface components; building, testing, and further refining an interface; before finally conducting usability tests of the system. Lessons are learned at every stage of the process leading to a much more informed view of how such an interface should be built

Which User Interaction for Cross-Language Information Retrieval? Design Issues and Reflections

A novel and complex form of information access is cross-language information retrieval: searching for texts written in foreign languages based on native language queries. Although the underlying technology for achieving such a search is relatively well understood, the appropriate interface design is not. This paper presents three user evaluations undertaken during the iterative design of Clarity, a cross-language retrieval system for rare languages, and shows how the user interaction design evolved depending on the results of usability tests. The first test was instrumental to identify weaknesses in both functionalities and interface; the second was run to determine if query translation should be shown or not; the final was a global assessment and focussed on user satisfaction criteria. Lessons were learned at every stage of the process leading to a much more informed view of what a cross-language retrieval system should offer to users

Which user interaction for cross-language information retrieval? Design issues and reflections

A novel and complex form of information access is cross-language information retrieval: searching for texts written in foreign languages based on native language queries. Although the underlying technology for achieving such a search is relatively well understood, the appropriate interface design is not. The authors present three user evaluations undertaken during the iterative design of Clarity, a cross-language retrieval system for low-density languages, and shows how the user-interaction design evolved depending on the results of usability tests. The first test was instrumental to identify weaknesses in both functionalities and interface; the second was run to determine if query translation should be shown or not; the final was a global assessment and focused on user satisfaction criteria. Lessons were learned at every stage of the process leading to a much more informed view of what a cross-language retrieval system should offer to users

Gestores de contenido en la arquitectura de la producción multiplataforma. El caso de Televisió de Catalunya

Se analiza un gestor de contenidos adaptado a las necesidades de Televisión de Catalunya a partir de un software que se comercializó con el nombre de DeliverTy, como sistema tecnológico para la producción audiovisual multiplataforma. Se plantean las características de este software, las ventajas que conlleva su implementación y se describen las implicaciones que se derivan de su uso en las diferentes fases e producción de contenidos multiplataforma. Del análisis se extraen conclusiones sobre las contribuciones de los gestores de contenidos a la producción de la información. Entre las ventajas del sistema se puede destacar la simplificación de las tareas de administración de los documentos y archivos generados por los trabajadores del medio televisivo y la facilidad para derivar dichos contenidos hacia las nuevas plataformas.This article examines an software adapted in-house from DeliverTy, a content management system, as a technological system for the multiplatform audiovisual production of the autonomous television network of Catalonia. We analyze the features of this software, discuss the benefits associated with its use, and elaborate on the different phases of digital media production. The study draws conclusions on the contribution of content management systems to information production. Among the advantages that stand out are the simplification of the tasks related to document management and files generation, as well as the simplification of the process to broadcast these contents on new platforms