123 research outputs found

    Review of Impacts of Leadership Competence of Project Managers on Construction Project Success

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    Leadership - Human endeavors play an important role in all areas of socio-economic development for any country in the world. Construction investment projects need more leadership role by their own characteristics. This research aims to give an overview of the theories of project manager leadership competence, the viewpoints of evaluation of the project's success, and the views on the influence of leadership competences and the success of the project. Research on the use of reliable secondary document sources in the world is synthesized from research works, scientific reports in the field of project management. The results of this study show that there is a great deal of research literature on project manager leadership competence and the success of the project in general but not many research papers on construction projects. This research is intended to suggest further studies to determine the specific influences, from that the training direction will increase the project management competence in the field of construction

    Role of GmNAC019 transcription factor in salinity and drought tolerance of transgenic Arabidopsis thaliana

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    Increasingly severe drought and salinity stress due to global climate change have made these stresses bigger threats to ecosystem and agriculture. Previous studies reported that GmNAC019, a soybean NAC transcription factor - encoding gene, displayed induced expression upon drought treatment in wild-type cultivars. In this study, drought and salinity stresses were applied on GmNAC019-overexpressing Arabidopsis plants to verify the contribution of GmNAC019 in regulating plant response to the stress conditions. Results from the water loss rate and survival rate assays revealed that the transgenic line conferred improved tolerance to drought stress as evidenced by lower leaf water loss and significantly higher rate of survival than seen in the wild-type plants. Similarly, the survival rate assay for testing salinity effects on plants by growing the plants on MS medium supplemented with different NaCl concentrations also indicated that the transgenic plants had a better tolerance to salt stress as they displayed lower rate of root growth inhibition and higher survival rate. Taken these results altogether, it is suggested that GmNAC019 might play important role in aiding plant response to drought and salinity stresses. Specific functions of this gene should be elaborated in future studies to evaluate its potential application for crop improvement

    Isolation an agar degradation Bacillus sp. AT6 and preliminary application for seaweed saccharification

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    Background: Agar is a common polysaccharide found in nature. However, agar is strongly resisted to the degradation processing, leading to limitation of its application in various areas. Thus, finding an effective solution for agar saccharification significantly improves the economically effects of agar based substrates.Methods: Soil samples were collected from TienPhong Forestry Ltd. Company, ThuyXuan District, ThuaThien Hue province, Vietnam. Potential agar degrading bacteria were screened on a mineral salt agar medium. The isolate was identified based on 16S rRNA nucleotide sequence, morphological, physiological, and biochemical characteristics. Agarase production was evaluated by modification culture conditions including incubation time, shaking speed, and initial inoculum size. Molecular mass of extracellular agarase was determined by native SDS-PAGE. The effect of pH, temperature, metal ions, and organic solvents were conducted for enzyme characterization. Application of enzyme was investigated on seaweed saccharification.Result: An agar degrading bacterial strain was isolated from soils and identified as Bacillus sp. AT6. Maximal agarase accumulation obtained in the culture containing an inoculum size of 10% (v/v), shaking speed of 210 rpm, and 96 hours incubation. The agarase revealed a single band on zymogram analysis with an apparent molecular weight of 180 kDa. The optimal temperature and pH were 40°C and pH 8.0, respectively. All tested metal ions and organic solvents partially decreased enzyme activity. Treatment seaweed by agarase resulted in reducing sugars release present in the reaction, indicating the saccharification of seaweed was succeeded.Conclusion: Bacillus sp. AT6 is a new report of agarolytic bacteria that produces extracellular agarase enzymes. The present results promise strain AT6 is a great candidate for agar saccharification for industrial application

    An HPLC Quantitative Analysis of Paraquat in Human Plasma: A Helpful Tool for Diagnosis and Evaluation of Treatment of Paraquat Poisoning in Vietnamese Hospitals

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    In this paper, the paraquat (PQ) concentrations in plasma of poisoned patients were determined by high performance liquid chromatography (RP-HPLC) with a DAD detector followed simple extraction of PQ from plasma. The sample was simply pretreated with 15% trichloroacetic acid for deproteinization and directly injected to HPLC system. PQ in plasma was separated on a C8 column HPLC system using 2 channel mobile phase (A and B) with a volume ratio of 5:95, respectively. Channel A was 5% acetonitrile (ACN) and Channel B was a mixture of phosphate buffer (pH 2.5), sodium 1-heptanesulphonate (0.11% w / v), KCl (0.20% w / v), polyethylene glycol G400 (0.20% v / v) and methanol (20% v / v). The flow rate of mobile phase was 0.5 mL/min. The method detection limit (MDL) is 0.013 ?g/ mL and the quantitative limit is 0.040 ?g/ mL. The recovery of PQ in plasma samples was 96.1% - 105.9 % at 5 different concentrations ranging from 0.040 ?g / mL to 10.00 ?g/ mL. The within- and between-day relative standard deviations were all less than 0.82% and 1.43% respectively. The method was successfully applied for determining paraquat concentrations in plasma samples of 31 acute paraquat poisoned patients at Poison Control Center, Bach Mai hospital, Vietnam. Quantitative results revealed that plasma PQ level was a key factor for prognosis and hemoperfusion using resin membrane had significant effect in removing PQ from the blood

    Rural livelihood diversification in the South Central Coast of Vietnam

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    Agriculture is still a key livelihood earning for most rural households and working for wages does not seem to dramatically increase household income. Moreover, diversification of income sources is found to be influenced by these variables: age of household head, female head, ethnicity of household head, number of dependents, household labor, and community infrastructure. In addition, household labors, and average years of education have positive and statistically significant effects on household income. Households need to be acquainted with educational and training programs. These factors should be considered in policy decisions towards enhancing household income in the region

    Estimating Water Content and Grain Size of Intertidal Flat Sediments Using Visible to Shortwave-Infrared Reflectance and Sentinel 2A Data: A Case Study of the Red River Delta, Vietnam

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    Sediment properties such as water content (WC) and grain size (GS) are essential to characterize the environmental conditions of tidal flats. This article aimed to develop appropriate models to estimate the WC and GS of surface sediments for an intertidal flat on the Red river delta (Vietnam) using Sentinel 2A (S2A) images. The spectral reflectance, WC, and GS of 96 sub-samples from 12 sediment samples collected on December 17, 2017 were measured to clarify their relationships. The WC was highly correlated with the reflectance ratio of two shortwave-infrared bands, R(2190)/R(1610) (R² = 0.93). The median GS (D₅₀) at 0%, 15%, and 20% of WC was significantly correlated with the reflectance ratio of the near-infrared band (842 nm) versus the visible-green band (560 nm) (R² > 0.78). Next, D₅₀ was estimated from a multivariate regression model using this band ratio, the visible-red band (665 nm), and WC. The accuracy of the models was verified by comparisons with WC and D₅₀ from 20 samples collected on March 12th 2019 (RMSE of both WC and D₅₀ 30%) in very fine sediments (silts), which is consistent with other intertidal flats with similar sediment types. This article was limited to fine sediment samples. Therefore, our next step is to incorporate coarse sediments into the models to provide more universal mapping of WC and sediment types

    The impacts of lead recycling activities to human health and environment in Dong Mai craft village, Hung Yen, Vietnam

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    Lead (Pb) recycling activities in Dong Mai village, Chi Dao commune, Van Lam district, Hung Yen province have been taking place for more than 30 years. The development of recycling activities contributed to the improvement of the local economics. However, along with economic development, Dong Mai craft village is facing to serious pollution. Soil, air and water polluted by lead (Pb) caused food containing Pb at higher levels than allowed limit from 20 to 40 times. The pollution had the bad effect on human health in this village. The investigation results showed that 100 % of employee who participated on melting lead and 63.5 % of local children were poisoned by Pb. Besides, the local people got diseases relating to skin, eyes, etc. This situation requires the provincial authorities to find immediate solutions to reduce the impacts of Pb recycling activities to environment and human.Hoạt động tái chế chì của thôn Đông Mai, xã Chỉ Đạo, huyện Văn Lâm, tỉnh Hưng Yên đã diễn ra hơn 30 năm nay. Sự phát triển của nghề tái chế đã làm góp phần cải thiện kinh tế của địa phương. Tuy nhiên, cùng với sự phát triển kinh tế, làng nghề Đông Mai đã và đang đối mặt với ô nhiễm môi trường nghiêm trọng. Môi trường đất, nước không khí bị ô nhiễm bởi chì (Pb) dẫn đến thực phẩm nhiễm chì ở hàm lượng cao hơn mức cho phép từ 20- 40 lần. Sự ô nhiễm gây ra những ảnh hưởng xấu tới sức khỏe của người dân làng nghề. Kết quả điều tra cho thấy 100% công nhân tham gia nấu chì và 63.5% trẻ em địa phương bị nhiễm độc chì. Bên cạnh đó, người dân địa phương còn bị mắc của các loại bệnh ngoài da, bệnh về mắt, …. Tình trạng này đòi hỏi chính quyền địa phương phải tìm ra giải pháp khẩn cấp để giảm thiểu tác động của những hoạt động tái chế chì tới môi trường và con người

    Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco

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    Tobacco is an important commercial crop and a rich source of alkaloids for pharmaceutical and agricultural applications. However, its yield can be reduced by up to 70% due to virus infections, especially by a potyvirus Potato virus Y (PVY). The replication of PVY relies on host factors, and eukaryotic translation initiation factor 4Es (eIF4Es) have already been identified as recessive resistance genes against potyviruses in many plant species. To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two clades, eIF4E1 (eIF4E1-S and eIF4E1-T) and eIF4E2 (eIF4E2-S and eIF4E2-T) in the eIF4E gene family comprising six members in tobacco. We screened for CRISPR/Cas9-induced mutations by heteroduplex analysis and Sanger sequencing, and monitored PVY(O) accumulation in virus challenged regenerated plants by DAS-ELISA both in T0 and T1 generations. We found that all T0 lines carrying targeted mutations in the eIF4E1-S gene displayed enhanced resistance to PVY(O) confirming previous reports. More importantly, our combinatorial approach revealed that eIF4E1-S is necessary but not sufficient for complete PVY resistance. Only the quadruple mutants harboring loss-of-function mutations in eIF4E1-S, eIF4E1-T, eIF4E2-S and eIF4E2-T showed heritable high-level resistance to PVY(O) in tobacco. Our work highlights the importance of understanding host factor redundancy in virus replication and provides a roadmap to generate virus resistance by combinatorial CRISPR/Cas9-mediated editing in non-model crop plants with complex genomes
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