The role of bacteria in pine wilt disease: insights from microbiome analysis.

Pine Wilt Disease (PWD) has a significant impact on Eurasia pine forests. The microbiome of the nematode (the primary cause of the disease), its insect vector, and the host tree may be relevant for the disease mechanism. The aim of this study was to characterize these microbiomes, from three PWD-affected areas in Portugal, using Denaturing Gradient Gel Electrophoresis, 16S rRNA gene pyrosequencing, and a functional inference-based approach (PICRUSt). The bacterial community structure of the nematode was significantly different from the infected trees but closely related to the insect vector, supporting the hypothesis that the nematode microbiome might be in part inherited from the insect. Sampling location influenced mostly the tree microbiome (P < 0.05). Genes related both with plant growth promotion and phytopathogenicity were predicted for the tree microbiome. Xenobiotic degradation functions were predicted in the nematode and insect microbiomes. Phytotoxin biosynthesis was also predicted for the nematode microbiome, supporting the theory of a direct contribution of the microbiome to tree-wilting. This is the first study that simultaneously characterized the nematode, tree and insect-vector microbiomes from the same affected areas, and overall the results support the hypothesis that the PWD microbiome plays an important role in the disease's development

Torque Ripple Minimization in a Switched Reluctance Drive by Neuro-Fuzzy Compensation

Simple power electronic drive circuit and fault tolerance of converter are specific advantages of SRM drives, but excessive torque ripple has limited its use to special applications. It is well known that controlling the current shape adequately can minimize the torque ripple. This paper presents a new method for shaping the motor currents to minimize the torque ripple, using a neuro-fuzzy compensator. In the proposed method, a compensating signal is added to the output of a PI controller, in a current-regulated speed control loop. Numerical results are presented in this paper, with an analysis of the effects of changing the form of the membership function of the neuro-fuzzy compensator.Comment: To be published in IEEE Trans. on Magnetics, 200

Crossed Module Bundle Gerbes; Classification, String Group and Differential Geometry

We discuss nonabelian bundle gerbes and their differential geometry using simplicial methods. Associated to any crossed module there is a simplicial group NC, the nerve of the 1-category defined by the crossed module and its geometric realization |NC|. Equivalence classes of principal bundles with structure group |NC| are shown to be one-to-one with stable equivalence classes of what we call crossed module gerbes bundle gerbes. We can also associate to a crossed module a 2-category C'. Then there are two equivalent ways how to view classifying spaces of NC-bundles and hence of |NC|-bundles and crossed module bundle gerbes. We can either apply the W-construction to NC or take the nerve of the 2-category C'. We discuss the string group and string structures from this point of view. Also a simplicial principal bundle can be equipped with a simplicial connection and a B-field. It is shown how in the case of a simplicial principal NC-bundle these simplicial objects give the bundle gerbe connection and the bundle gerbe B-field

Beyond Correlation Filters: Learning Continuous Convolution Operators for Visual Tracking

Discriminative Correlation Filters (DCF) have demonstrated excellent performance for visual object tracking. The key to their success is the ability to efficiently exploit available negative data by including all shifted versions of a training sample. However, the underlying DCF formulation is restricted to single-resolution feature maps, significantly limiting its potential. In this paper, we go beyond the conventional DCF framework and introduce a novel formulation for training continuous convolution filters. We employ an implicit interpolation model to pose the learning problem in the continuous spatial domain. Our proposed formulation enables efficient integration of multi-resolution deep feature maps, leading to superior results on three object tracking benchmarks: OTB-2015 (+5.1% in mean OP), Temple-Color (+4.6% in mean OP), and VOT2015 (20% relative reduction in failure rate). Additionally, our approach is capable of sub-pixel localization, crucial for the task of accurate feature point tracking. We also demonstrate the effectiveness of our learning formulation in extensive feature point tracking experiments. Code and supplementary material are available at http://www.cvl.isy.liu.se/research/objrec/visualtracking/conttrack/index.html.Comment: Accepted at ECCV 201

Measurement of miniband parameters of a doped superlattice by photoluminescence in high magnetic fields

We have studied a 50/50\AA superlattice of GaAs/Al$_{0.21}$Ga$_{0.79}$As composition, modulation-doped with Si, to produce $n=1.4\times 10^{12}$ cm$^{-2}$ electrons per superlattice period. The modulation-doping was tailored to avoid the formation of Tamm states, and photoluminescence due to interband transitions from extended superlattice states was detected. By studying the effects of a quantizing magnetic field on the superlattice photoluminescence, the miniband energy width, the reduced effective mass of the electron-hole pair, and the band gap renormalization could be deduced.Comment: minor typing errors (minus sign in eq. (5)

O sistema dois-híbridos de Saccharomyces cerevisiae.

Variações e potencial para a análise funcional e montagem de redes de interações proteína-proteína

Sharp lines in the absorption edge of EuTe and Pb0.1_{0.1}Eu0.9_{0.9}Te in high magnetic fields

The optical absorption spectra in the region of the \fd transition energies of epitaxial layers of of EuTe and \PbEuTe, grown by molecular beam epitaxy, were studied using circularly polarized light, in the Faraday configuration. Under \sigmam polarization a sharp symmetric absorption line (full width at half-maximum 0.041 eV) emerges at the low energy side of the band-edge absorption, for magnetic fields intensities greater than 6 T. The absorption line shows a huge red shift (35 meV/T) with increasing magnetic fields. The peak position of the absorption line as a function of magnetic field is dominated by the {\em d-f} exchange interaction of the excited electron and the \Euion spins in the lattice. The {\em d-f} exchange interaction energy was estimated to be $J_{df}S=0.15\pm 0.01$ eV. In \PbEuTe the same absorption line is detected, but it is broader, due to alloy disorder, indicating that the excitation is localized within a finite radius. From a comparison of the absorption spectra in EuTe and \PbEuTe the characteristic radius of the excitation is estimated to be $\sim 10$\AA.Comment: Journal of Physics: Condensed Matter (2004, at press

Galaxy formation in the Planck cosmology - II. Star-formation histories and post-processing magnitude reconstruction

We adapt the L-Galaxies semi-analytic model to follow the star-formation histories (SFH) of galaxies -- by which we mean a record of the formation time and metallicities of the stars that are present in each galaxy at a given time. We use these to construct stellar spectra in post-processing, which offers large efficiency savings and allows user-defined spectral bands and dust models to be applied to data stored in the Millennium data repository. We contrast model SFHs from the Millennium Simulation with observed ones from the VESPA algorithm as applied to the SDSS-7 catalogue. The overall agreement is good, with both simulated and SDSS galaxies showing a steeper SFH with increased stellar mass. The SFHs of blue and red galaxies, however, show poor agreement between data and simulations, which may indicate that the termination of star formation is too abrupt in the models. The mean star-formation rate (SFR) of model galaxies is well-defined and is accurately modelled by a double power law at all redshifts: SFR proportional to $1/(x^{-1.39}+x^{1.33})$, where $x=(t_a-t)/3.0\,$Gyr, $t$ is the age of the stars and $t_a$ is the loopback time to the onset of galaxy formation; above a redshift of unity, this is well approximated by a gamma function: SFR proportional to $x^{1.5}e^{-x}$, where $x=(t_a-t)/2.0\,$Gyr. Individual galaxies, however, show a wide dispersion about this mean. When split by mass, the SFR peaks earlier for high-mass galaxies than for lower-mass ones, and we interpret this downsizing as a mass-dependence in the evolution of the quenched fraction: the SFHs of star-forming galaxies show only a weak mass dependence.Comment: Accepted version of the paper, to appear in MNRAS. Compared to the original version, contains more detail on the post-processing of magnitudes, including a table of rms magnitude errors. SFHs available on Millennium database http://gavo.mpa-garching.mpg.de/MyMillennium

The complexity of porphyrin-like pigments in a marine annelid sheds new light on haem metabolism in aquatic invertebrates

PTDC/MAR-BIO/0113/2014. UID/Multi/04378/2019. UID/QUI/50006/2019. SFRH/BD/120030/2016. SFRH/BD/109462/2015. IF/00265/2015.True green pigments in the animal kingdom are scarce and are almost invariably porphyrinoids. Endogenous porphyrins resulting from the breakdown of haem are usually known as “bile pigments”. The pigmentation of intertidal Polychaeta has long gained attention due to its variety and vivid patterning that often seems incompatible with camouflage, as it occurs with Eulalia viridis, one of the few truly green Polychaeta. The present study combined UV and bright-field microscopy with HPLC to address the presence and distribution of pigments in several organs. The results showed two major types of porphyrin-like pigments, yellowish and greenish in colour, that are chiefly stored as intraplasmatic granules. Whereas the proboscis holds yellow pigments, the skin harbours both types in highly specialised cells. In their turn, oocytes and intestine have mostly green pigments. Despite some inter-individual variation, the pigments tend to be stable after prolonged storage at −20 °C, which has important implications for future studies. The results show that, in a foraging predator of the intertidal where melanins are circumscribed to lining the nervous system, porphyrinoid pigments have a key role in protection against UV light, in sensing and even as chemical defence against foulants and predators, which represents a remarkable adaptive feature.publishersversionpublishe