248 research outputs found
Conservation of binding site specificity of three yeast DNA binding proteins
AbstractSequence specific binding of protein extracts from 13 different yeast species to three oligonucleotide probes and two points mutants derived from Saccharomyces cerevisiae DNA binding proteins were tested using mobility shift assays. The probes were high affinity binding sites for GRF1/RAP1/ ABF1 and CP1/CPF1. Most yeasts in the genus Saccharomyces showed specific binding to all three probes and also displayed similar sequence requirements when challenged by molar excesses of mutant probes. The affinities for the probes varied amongst the other yeasts tested, but in general, CPF1 binding activity was the most widespread, while the other two were more limited
Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield
Open Access Article; Published online: 17 Jan 2020Cassava is an important staple crop in subâSaharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the CalvinâBenson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouseâgrown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement
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Analysis of energy-efficiency investment decisions by small and medium-sized manufacturers
This report highlights the results of a comprehensive analysis of investment decisions regarding energy-efficiency measures at small and medium-sized manufacturing plants. The analysis is based on the experiences of companies participating in the DOE Industrial Assessment Center (IAC) program. The IAC program is a network of university-based centers that provides energy and waste assessments to small and medium-sized manufacturing plants. The purposes of this report are to do the following: (1) Examine what the data collected reveal about patterns of implementation of recommended energy- efficiency measures, (2) Evaluate how various factors, such as the type of industry, the characteristics of the manufacturing plants, or the cost of the measures, appear to effect implementation rates, (3) Examine reasons why recommended energy-saving measures are accepted or rejected
A Current Induced Transition in atomic-sized contacts of metallic Alloys
We have measured conductance histograms of atomic point contacts made from
the noble-transition metal alloys CuNi, AgPd, and AuPt for a concentration
ratio of 1:1. For all alloys these histograms at low bias voltage (below 300
mV) resemble those of the noble metals whereas at high bias (above 300 mV) they
resemble those of the transition metals. We interpret this effect as a change
in the composition of the point contact with bias voltage. We discuss possible
explanations in terms of electromigration and differential diffusion induced by
current heating.Comment: 5 pages, 6 figure
Soil carbon sequestration in grazing systems : managing expectations
The inputs of C.M.G., M.H., and P.S. contribute to the project DEVIL [NE/M021327/1]. The input of P.S. also contributes to the following projects: U-GRASS [NE/M016900/1] and Soils-R-GRREAT [NE/P019455/1]. We thank the Centre of Organic Production and Consumption (EPOK) at the Swedish University of Agricultural Sciences for funding E.R.âs part of the research.Peer reviewedPostprin
Beautiful Mirrors and Precision Electroweak Data
The Standard Model (SM) with a light Higgs boson provides a very good
description of the precision electroweak observable data coming from the LEP,
SLD and Tevatron experiments. Most of the observables, with the notable
exception of the forward-backward asymmetry of the bottom quark, point towards
a Higgs mass far below its current experimental bound. The disagreement, within
the SM, between the values for the weak mixing angle as obtained from the
measurement of the leptonic and hadronic asymmetries at lepton colliders, may
be taken to indicate new physics contributions to the precision electroweak
observables. In this article we investigate the possibility that the inclusion
of additional bottom-like quarks could help resolve this discrepancy. Two
inequivalent assignments for these new quarks are analysed. The resultant fits
to the electroweak data show a significant improvement when compared to that
obtained in the SM. While in one of the examples analyzed, the exotic quarks
are predicted to be light, with masses below 300 GeV, and the Higgs tends to be
heavy, in the second one the Higgs is predicted to be light, with a mass below
250 GeV, while the quarks tend to be heavy, with masses of about 800 GeV. The
collider signatures associated with the new exotic quarks, as well as the
question of unification of couplings within these models and a possible
cosmological implication of the new physical degrees of freedom at the weak
scale are also discussed.Comment: 21 pages, 4 embedded postscript figures, LaTeX. Two minor corrections
performe
Phenomenological constraints on SUSY SU(5) GUTs with non-universal gaugino masses
We study phenomenological aspects of supersymmetric SU(5) grand unified
theories with non-universal gaugino masses. For large tan beta, we investigate
constraints from the requirement of successful electroweak symmetry breaking,
the positivity of stau mass squared and the b to s gamma decay rate. In the
allowed region, the nature of the lightest supersymmetric particle is
determined. Examples of mass spectra are given. We also calculate loop
corrections to the bottom mass due to superpartners.Comment: 10 pages, 2 figures (8 eps files), uses REVTeX. Replaced to match the
version to be published in PRD: minor corrections and addition
Defining a land boundary for sustainable livestock consumption
The need for more sustainable production and consumption of animal source food (ASF) is central to the achievement of the sustainable development goals: within this context, wise use of land is a core challenge and concern. A key question in feeding the future world is: how much ASF should we eat? We demonstrate that livestock raised under the circular economy concept could provide a significant, nonnegligible part (9â23 g/per capita) of our daily protein needs (~50â60 g/per capita). This livestock then would not consume human-edible biomass, such as grains, but mainly convert leftovers from arable land and grass resources into valuable food, implying that production of livestock feed is largely decoupled from arable land. The availability of these biomass streams for livestock then determines the boundaries for livestock production and consumption. Under this concept, the competition for land for feed or food would be minimized and compared to no ASF, including some ASF in the human diet could free up about one quarter of global arable land. Our results also demonstrate that restricted growth in consumption of ASF in Africa and Asia would be feasible under these boundary conditions, while reductions in the rest of the world would be necessary to meet land use sustainability criteria. Managing this expansion and contraction of future consumption of ASF is essential for achieving sustainable nutrition security.</p
Phenomenology of flavor-mediated supersymmetry breaking
The phenomenology of a new economical SUSY model that utilizes dynamical SUSY
breaking and gauge-mediation (GM) for the generation of the sparticle spectrum
and the hierarchy of fermion masses is discussed. Similarities between the
communication of SUSY breaking through a messenger sector, and the generation
of flavor using the Froggatt-Nielsen (FN) mechanism are exploited, leading to
the identification of vector-like messenger fields with FN fields, and the
messenger U(1) as a flavor symmetry. An immediate consequence is that the first
and second generation scalars acquire flavor-dependent masses, but do not
violate FCNC bounds since their mass scale, consistent with effective SUSY, is
of order 10 TeV. We define and advocate a minimal flavor-mediated model (MFMM),
recently introduced in the literature, that successfully accommodates the small
flavor-breaking parameters of the standard model using order one couplings and
ratios of flavon field vevs. The mediation of SUSY breaking occurs via two-loop
log-enhanced GM contributions, as well as several one-loop and two-loop
Yukawa-mediated contributions for which we provide analytical expressions. The
MFMM is parameterized by a small set of masses and couplings, with values
restricted by several model constraints and experimental data. The
next-to-lightest sparticle (NLSP) always has a decay length that is larger than
the scale of a detector, and is either the lightest stau or the lightest
neutralino. Similar to ordinary GM models, the best collider search strategies
are, respectively, inclusive production of at least one highly ionizing track,
or events with many taus plus missing energy. In addition, D^0 - \bar{D}^0
mixing is also a generic low energy signal. Finally, the dynamical generation
of the neutrino masses is briefly discussed.Comment: 54 pages, LaTeX, 8 figure
Energy Flow in the Hadronic Final State of Diffractive and Non-Diffractive Deep-Inelastic Scattering at HERA
An investigation of the hadronic final state in diffractive and
non--diffractive deep--inelastic electron--proton scattering at HERA is
presented, where diffractive data are selected experimentally by demanding a
large gap in pseudo --rapidity around the proton remnant direction. The
transverse energy flow in the hadronic final state is evaluated using a set of
estimators which quantify topological properties. Using available Monte Carlo
QCD calculations, it is demonstrated that the final state in diffractive DIS
exhibits the features expected if the interaction is interpreted as the
scattering of an electron off a current quark with associated effects of
perturbative QCD. A model in which deep--inelastic diffraction is taken to be
the exchange of a pomeron with partonic structure is found to reproduce the
measurements well. Models for deep--inelastic scattering, in which a
sizeable diffractive contribution is present because of non--perturbative
effects in the production of the hadronic final state, reproduce the general
tendencies of the data but in all give a worse description.Comment: 22 pages, latex, 6 Figures appended as uuencoded fil
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