Transcriptional responses of Leishmania (Leishmania) amazonensis in the presence of trivalent sodium stibogluconate

Background: In the last decade, resistance to antimonials has become a serious problem due to the emergence of drug-resistant strains. Therefore, understanding the mechanisms used by Leishmania parasites to survive under drug pressure is essential, particularly for species of medical-veterinary importance such as L. amazonensis. Methods: Here, we used RNA-seq technology to analyse transcriptome profiles and identify global changes in gene expression between antimony-resistant and -sensitive L. amazonensis promastigotes. Results: A total of 723 differentially expressed genes were identified between resistant and sensitive lines. Comparative transcriptomic analysis revealed that genes encoding proteins involved in metabolism (fatty acids) and stress response, as well as those associated with antimony resistance in other Leishmania species, were upregulated in the antimony-resistant line. Most importantly, we observed upregulation of genes encoding autophagy proteins, suggesting that in the presence of trivalent stibogluconate (SbIII) L. amazonensis can activate these genes either as a survival strategy or to induce cell death, as has been observed in other parasites. Conclusions: This work identified global transcriptomic changes in an in vitro-adapted strain in response to SbIII. Our results provide relevant information to continue understanding the mechanism used by parasites of the subgenus Leishmania (L. amazonensis) to generate an antimony-resistant phenotype. © 2019 The Author(s)

Using LiDAR to assess the effect of fire and floods on upland peat bogs, Waterfall Gully, Mount Lofty Ranges, South Australia

A flood exceeding the 100 year average recurrence interval in November 2005 led to the failure of an upland peat bog in Waterfall Gully. The area is prone to severe bushfire and flood events and the control dam at the base of First Falls was filled with sediment sourced from Wilson Bog. A resistant quartzite bar at Fourth Falls has formed a natural constriction point against which burnt logs and debris have collected following previous fire events forming a natural dam resulting in sediment/peat accumulation upstream. The failure of the bog was inevitable as the vegetative material in the log-jam progressively weakened and rotted. Intense flooding triggered the failure but it was augmented by the build up of a critical mass of sediment upstream of the restriction point. The downstream force of the flood waters and the weight of the saturated bog sediments was enough to overcome the basal frictional forces resulting in slumping and headward erosion. LiDAR data clearly shows an erosion channel scoured out by the flood. Approximately 5000 m3 of sediment (-10,100 tonnes) was washed downstream. LiDAR coupled with a tri-spectral scanner has the capacity to identify other upland peat bogs due to their high NDVI value and assess their stability on steep slopes or narrow valleys. Fire is another risk to the stability of these bogs as it has the potential to remove binding vegetation and expose unconsolidated sediments to erosion during subsequent rain events. Groundsurface and vegetation surface DEM\u27s generated from LiDAR combined with NDVI maps derived from a tri-spectral scanner provide an ideal tool to monitor and assess the risk of slumping in other upland peat bogs

Intraspecific genomic divergence and minor structural variations in leishmania (Viannia) panamensis

Leishmania (Viannia) panamensis is one of the most important Leishmania species associated with cutaneous leishmaniasis (CL) in Latin America. Despite its wide geographic distribution and pathogenic potential in humans and animals, the genomic variability of this species is low compared with other Leishmania species circulating in the same geographical area. No studies have reported a detailed analysis of the whole genome of L. panamensis from clinical isolates using DNA highthroughput sequencing to clarify its intraspecific genomic variability or plausible divergence. Therefore, this study aimed to evaluate the intraspecific genomic variability of L. panamensis from Colombia and Panama. A total of 22 genomes were analyzed, 19 from Colombian patients with CL and three genomes from Panama obtained from public databases. The phylogenomic analysis revealed the potential existence of three well-supported clades as evidence of intraspecific divergence. Additionally, the whole-genome analysis showed low structural variations in terms of ploidy, copy number variations, and single-nucleotide polymorphisms (SNPs). SNPs shared among all clades were identified, revealing their importance in different biological processes of L. panamensis. The findings not only expand our knowledge of intraspecific genomic variability of one of the most important Leishmania species in South America but also highlights the possible existence of different clades/lineages/subpopulations across a geographic scale. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Spectral optical monitoring of the double peaked emission line AGN Arp 102B: II. Variability of the broad line properties

We investigate a long-term (26 years, from 1987 to 2013) variability in the broad spectral line properties of the radio galaxy Arp 102B, an active galaxy with broad double-peaked emission lines. We use observations presented in Paper I (Shapovalova et al. 2013) in the period from 1987 to 2011, and a new set of observations performed in 2012--2013. To explore the BLR geometry, and clarify some contradictions about the nature of the BLR in Arp 102B we explore variations in the H$\alpha$ and H$\beta$ line parameters during the monitored period. We fit the broad lines with three broad Gaussian functions finding the positions and intensities of the blue and red peaks in H$\alpha$ and H$\beta$. Additionally we fit averaged line profiles with the disc model. We find that the broad line profiles are double-peaked and have not been changed significantly in shapes, beside an additional small peak that, from time to time can be seen in the blue part of the H$\alpha$ line. The positions of the blue and red peaks { have not changed significantly during the monitored period. The H$\beta$ line is broader than H$\alpha$ line in the monitored period. The disc model is able to reproduce the H$\beta$ and H$\alpha$ broad line profiles, however, observed variability in the line parameters are not in a good agreement with the emission disc hypothesis. It seems that the BLR of Arp 102B has a disc-like geometry, but the role of an outflow can also play an important role in observed variation of the broad line properties.Comment: 17 pages, Accepted for publication in A&

Framework for classroom student grading with open-ended questions: A text-mining approach

The purpose of this paper is to present a framework based on text-mining techniques to support teachers in their tasks of grading texts, compositions, or essays, which form the answers to open-ended questions (OEQ). The approach assumes that OEQ must be used as a learning and evaluation instrument with increasing frequency. Given the time-consuming grading process for those questions, their large-scale use is only possible when computational tools can help the teacher. This work assumes that the grading decision is entirely a teacher’s task responsibility, not the result of an automatic grading process. In this context, the teacher is the author of questions to be included in the tests, administration and results assessment, the entire cycle for this process being noticeably short: a few days at most. An attempt is made to address this problem. The method is entirely exploratory, descriptive and data-driven, the only data assumed as inputs being the texts of essays and compositions created by the students when answering OEQ for a single test on a specific occasion. Typically, the process involves exceedingly small data volumes measured by the power of current home computers, but big data when compared with human capabilities. The general idea is to use software to extract useful features from texts, perform lengthy and complex statistical analyses and present the results to the teacher, who, it is believed, will combine this information with his or her knowledge and experience to make decisions on mark allocation. A generic path model is formulated to represent that specific context and the kind of decisions and tasks a teacher should perform, the estimated results being synthesised using graphic displays. The method is illustrated by analysing three corpora of 126 texts originating in three different real learning contexts, time periods, educational levels and disciplines.info:eu-repo/semantics/publishedVersio

OPTICAL SPECTROSCOPIC OBSERVATIONS OF GAMMA-RAY BLAZAR CANDIDATES. VI. FURTHER OBSERVATIONS FROM TNG, WHT, OAN, SOAR, AND MAGELLAN TELESCOPES

Indexación: Web of ScienceBlazars, one of the most extreme classes of active galaxies, constitute so far the largest known population of.-ray sources, and their number is continuously growing in the Fermi catalogs. However, in the latest release of the Fermi catalog there is still a large fraction of sources that are classified as blazar candidates of uncertain type (BCUs) for which optical spectroscopic observations are necessary to confirm their nature and their associations. In addition, about one-third of the gamma-ray point sources listed in the Third Fermi-LAT Source Catalog (3FGL) are still unassociated and lacking an assigned lower-energy counterpart. Since 2012 we have been carrying out an optical spectroscopic campaign to observe blazar candidates to confirm their nature. In this paper, the sixth of the series, we present optical spectroscopic observations for 30 gamma-ray blazar candidates from different observing programs we carried out with the Telescopio Nazionale Galileo, William Herschel Telescope, Observatorio Astronomico Nacional, Southern Astrophysical Research Telescope, and Magellan. Telescopes. We found that 21 out of 30 sources investigated are BL Lac objects, while the remaining targets are classified as flat-spectrum radio quasars showing the typical broad emission lines of normal quasi-stellar objects. We conclude that our selection of gamma-ray blazar. candidates based on their multifrequency properties continues to be a successful way to discover potential low-energy counterparts of the Fermi. unidentified gamma-ray sources and to confirm the nature of BCUs.http://iopscience.iop.org/article/10.3847/0004-6256/151/4/95/met

Study of Foaming Properties and Effect of the Isomeric Distribution of Some Anionic Surfactants

Using different reaction conditions of photosulfochlorination of n-dodecane, two samples of anionic surfactants of sulfonate type are obtained. Their micellar behavior has been already reported and the relationship between their isomeric distribution and their chemical structures and micellar behaviors have been more thoroughly explored. In this investigation, we screened the foaming properties (foaming power and foam stability) by a standardized method very similar to the Ross–Miles foaming tests to identify which surfactants are suitable for applications requiring high foaming, or, alternatively, low foaming. The results obtained for the synthesized surfactants are compared to those obtained for an industrial sample of secondary alkanesulfonate (Hostapur 60) and to those of a commercial sample of sodium dodecylsulfate used as reference for anionic surfactants. The foam formation and foam stability of aqueous solutions of the two samples of dodecanesulfonate are compared as a function of their isomeric distribution. These compounds show good foaming power characterized in most cases by metastable or dry foams. The highest foaming power is obtained for the sample rich in primary isomers which also produces foam with a relatively high stability. For the sample rich in secondary isomers we observe under fixed conditions a comparable initial foam height but the foam stability turns out to be low. This property is interesting for applications requiring low foaming properties such as dishwashing liquid for machines. The best results are observed near and above the critical micellar concentrations and at 25 C for both the samples

Characterisation of puroindoline genes in wild tetraploid and hexaploid wheats (Triticum araraticum; T. timopheevii and T. zhukovskyi)

Treatment with cisplatin-containing chemotherapy regimens causes hearing loss in 40-60% of cancer patients. It has been suggested that genetic variants in the genes encoding thiopurine S-methyltransferase (TPMT) and catechol O-methyltransferase (COMT) can predict the development of cisplatin-induced ototoxicity and may explain interindividual variability in sensitivity to cisplatin-induced hearing loss. Two recently published studies however, sought to validate these findings and showed inconsistent results. The aim of this study was to evaluate the role of polymorphisms in the TPMT and COMT genes in cisplatin-induced ototoxicity. Therefore we investigated two independent cohorts of 110 Dutch and 38 Spanish patients with osteosarcoma and performed a meta-analysis including all previously published studies resulting in a total population of 664 patients with cancer. With this largest meta-analysis performed to date, we show that the influence of TPMT and COMT on the development of cisplatin-induced hearing loss may be less important than previously suggested