Uncertainties and speculations about the life cycle of the eastern oyster pathogen Haplosporidium nelsoni (MSX)

For 30 years, the pathogen Haplosporidium nelsoni (MSX) has been causing serious mortalities of eastern oysters Crassostrea virginica in the Delaware and Chesapeake bays of the eastern USA. Its life cycle is largely unknown, and methods for control are wanting. Breeding of resistant eastern oyster strains, at this time, offers the best hope for some degree of control of the disease. Although haplosporidians are known by their spores, controlled transmission, with one possible exception, has not been achieved in any of the 30 recognized species. Haplosporidium nelsoni rarely sporulates in eastern oysters, and this and other observations led to early speculation that another host probably exists. Based on apparent effects of environment on H. nelsoni abundance, particularly in Delaware Bay, we are attempting to profile a hypothetical alternate host. Recent increases in abundance and activity of MSX in Chesapeake and Delaware bays and in Virginia rivers are associated with droughts, but this is not true elsewhere. Distribution of H. nelsoni along the Atlantic coast appears to have spread slowly southward from Chesapeake and Delaware bays, but is not identified with eastern oyster mortalities in southern localities. To the north of the original epizootics, H. nelsoni has been scattered along the Long Island, Connecticut, and Massachusetts coasts for 30 years or more, usually without occurrence of serious mortalities of eastern oysters. Foci of increasing H. nelsoni activity with mortality, over the past 5 years, are of great concern in these areas. Careful examination of changes in these northern areas may contribute significantly to our understanding of the relationships between H. nelsoni and its environment.https://scholarworks.wm.edu/vimsbooks/1117/thumbnail.jp

Gamma radiation survey of the LDEF spacecraft

The retrieval of the Long Duration Exposure Facility spacecraft in January 1990 after nearly six years in orbit offered a unique opportunity to study the long term buildup of induced radioactivity in the variety of materials on board. We conducted the first complete gamma-ray survey of a large spacecraft on LDEF shortly after its return to earth. A surprising observation was the Be-7 activity which was seen primarily on the leading edge of the satellite, implying that it was picked up by LDEF in orbit. This is the first known evidence for accretion of a radioactive isotope onto an orbiting spacecraft. Other isotopes observed during the survey, the strongest being Na-22, are all attributed to activation of spacecraft components. Be-7 is a spallation product of cosmic rays on nitrogen and oxygen in the upper atmosphere. However, the observed density is much greater than expected due to cosmic-ray production in situ. This implies transport of Be-7 from much lower altitudes up to the LDEF orbit

Curvature invariants in type N spacetimes

Scalar curvature invariants are studied in type N solutions of vacuum Einstein's equations with in general non-vanishing cosmological constant Lambda. Zero-order invariants which include only the metric and Weyl (Riemann) tensor either vanish, or are constants depending on Lambda. Even all higher-order invariants containing covariant derivatives of the Weyl (Riemann) tensor are shown to be trivial if a type N spacetime admits a non-expanding and non-twisting null geodesic congruence. However, in the case of expanding type N spacetimes we discover a non-vanishing scalar invariant which is quartic in the second derivatives of the Riemann tensor. We use this invariant to demonstrate that both linearized and the third order type N twisting solutions recently discussed in literature contain singularities at large distances and thus cannot describe radiation fields outside bounded sources.Comment: 17 pages, to appear in Class. Quantum Gra

Developmental contexts and features of elite academy football players: Coach and player perspectives

Player profiling can reap many benefits; through reflective coach-athlete dialogue that produces a profile the athlete has a raised awareness of their own development, while the coach has an opportunity to understand the athlete's viewpoint. In this study, we explored how coaches and players perceived the development features of an elite academy footballer and the contexts in which these features are revealed, in order to develop a player profile to be used for mentoring players. Using a Delphi polling technique, coaches and players experienced a number of 'rounds' of expressing their opinions regarding player development contexts and features, ultimately reduced into a consensus. Players and coaches had differing priorities on the key contexts of player development. These contexts, when they reflect the consensus between players and coaches were heavily dominated by ability within the game and training. Personal, social, school, and lifestyle contexts featured less prominently. Although 'discipline' was frequently mentioned as an important player development feature, coaches and players disagreed on the importance of 'training'

Breathers in the weakly coupled topological discrete sine-Gordon system

Existence of breather (spatially localized, time periodic, oscillatory) solutions of the topological discrete sine-Gordon (TDSG) system, in the regime of weak coupling, is proved. The novelty of this result is that, unlike the systems previously considered in studies of discrete breathers, the TDSG system does not decouple into independent oscillator units in the weak coupling limit. The results of a systematic numerical study of these breathers are presented, including breather initial profiles and a portrait of their domain of existence in the frequency-coupling parameter space. It is found that the breathers are uniformly qualitatively different from those found in conventional spatially discrete systems.Comment: 19 pages, 4 figures. Section 4 (numerical analysis) completely rewritte

Anthropogenic Control over Wintertime Oxidation of Atmospheric Pollutants

Anthropogenic air pollutants such as nitrogen oxides (NO(x) = NO + NO(2)), sulfur dioxide (SO(2)), and volatile organic compounds (VOC), among others, are emitted to the atmosphere throughout the year from energy production and use, transportation, and agriculture. These primary pollutants lead to the formation of secondary pollutants such as fine particulate matter (PM(2.5)) and ozone (O(3)) and perturbations to the abundance and lifetimes of short-lived greenhouse gases. Free radical oxidation reactions driven by solar radiation govern the atmospheric lifetimes and transformations of most primary pollutants and thus their spatial distributions. During winter in the mid and high latitudes, where a large fraction of atmospheric pollutants are emitted globally, such photochemical oxidation is significantly slower. Using observations from a highly instrumented aircraft, we show that multi-phase reactions between gas-phase NO(x) reservoirs and aerosol particles, as well as VOC emissions from anthropogenic activities, lead to a suite of atypical radical precursors dominating the oxidizing capacity in polluted winter air, and thus, the distribution and fate of primary pollutants on a regional to global scale

Adiabatic limit and the slow motion of vortices in a Chern-Simons-Schr\"odinger system

We study a nonlinear system of partial differential equations in which a complex field (the Higgs field) evolves according to a nonlinear Schroedinger equation, coupled to an electromagnetic field whose time evolution is determined by a Chern-Simons term in the action. In two space dimensions, the Chern-Simons dynamics is a Galileo invariant evolution for A, which is an interesting alternative to the Lorentz invariant Maxwell evolution, and is finding increasing numbers of applications in two dimensional condensed matter field theory. The system we study, introduced by Manton, is a special case (for constant external magnetic field, and a point interaction) of the effective field theory of Zhang, Hansson and Kivelson arising in studies of the fractional quantum Hall effect. From the mathematical perspective the system is a natural gauge invariant generalization of the nonlinear Schroedinger equation, which is also Galileo invariant and admits a self-dual structure with a resulting large space of topological solitons (the moduli space of self-dual Ginzburg-Landau vortices). We prove a theorem describing the adiabatic approximation of this system by a Hamiltonian system on the moduli space. The approximation holds for values of the Higgs self-coupling constant close to the self-dual (Bogomolny) value of 1. The viability of the approximation scheme depends upon the fact that self-dual vortices form a symplectic submanifold of the phase space (modulo gauge invariance). The theorem provides a rigorous description of slow vortex dynamics in the near self-dual limit.Comment: Minor typos corrected, one reference added and DOI give

Agreement was moderate between data-based and opinion-based assessments of biases affecting randomised trials within meta-analyses

BACKGROUND: Randomised trials included in meta-analyses are often affected by bias caused by methodological flaws or limitations, but the degree of bias is unknown. Two proposed methods adjust trial results for bias using: (1) empirical evidence from published meta-epidemiological studies; or (2) expert opinion. METHODS: We investigated agreement between data-based and opinion-based approaches to assessing bias in each of four domains: sequence generation, allocation concealment, blinding and incomplete outcome data. From each sampled meta-analysis, a pair of trials with the highest and lowest empirical model-based bias estimates was selected. Independent assessors were asked which trial within each pair was judged more biased on the basis of detailed trial design summaries. RESULTS: Assessors judged trials to be equally biased in 68% of pairs evaluated. When assessors judged one trial as more biased, the proportion of judgements agreeing with the model-based ranking was highest for allocation concealment (79%) and blinding (79%) and lower for sequence generation (59%) and incomplete outcome data (56%). CONCLUSIONS: Most trial pairs found to be discrepant empirically were judged to be equally biased by assessors. We found moderate agreement between opinion and data-based evidence in pairs where assessors ranked one trial as more biased

Inheritance, Biochemical Abnormalities, and Clinical Features of Feline Mucolipidosis II: The First Animal Model of Human I-Cell Disease

Mucolipidosis II (ML II), also called I-cell disease, is a unique lysosomal storage disease caused by deficient activity of the enzyme N-acetylglucosamine-1-phosphotransferase, which leads to a failure to internalize enzymes into lysosomes. We report on a colony of domestic shorthair cats with ML II that was established from a half-sibling male of an affected cat. Ten male and 9 female kittens out of 89 kittens in 26 litters born to clinically normal parents were affected; this is consistent with an autosomal recessive mode of inheritance. The activities of three lysosomal enzymes from affected kittens, compared to normal adult control cats, were high in serum (11-73 times normal) but low in cultured fibroblasts (9-56% of normal range) that contained inclusion bodies (I-cells), reflecting the unique enzyme defect in ML II. Serum lysosomal enzyme activities of adult obligate carriers were intermediate between normal and affected values. Clinical features in affected kittens were observed from birth and included failure to thrive, behavioral dullness, facial dysmorphia, and ataxia. Radiographic lesions included metaphyseal flaring, radial bowing, joint laxity, and vertebral fusion. In contrast to human ML II, diffuse retinal degeneration leading to blindness by 4 months of age was seen in affected kittens. All clinical signs were progressive and euthanasia or death invariably occurred within the first few days to 7 months of life, often due to upper respiratory disease or cardiac failure. The clinical and radiographic features, lysosomal enzyme activities, and mode of inheritance are homologous with ML II in humans. Feline ML II is currently the only animal model in which to study the pathogenesis of and therapeutic interventions for this unique storage diseas