228 research outputs found

    Analysis of "Midnight" Tracks in the Stardust Interstellar Dust Collector: Possible Discovery of a Contemporary Interstellar Dust Grain

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    In January 2006, the Stardust sample return capsule returned to Earth bearing the first solid samples from a primitive solar system body, Comet 81P/Wild2, and a collector dedicated to the capture and return of contemporary interstellar dust. Both collectors were approximately 0.1m(exp 2) in area and were composed of aerogel tiles (85% of the collecting area) and aluminum foils. The Stardust Interstellar Dust Collector (SIDC) was exposed to the interstellar dust stream for a total exposure factor of 20 m(exp 2) day. The Stardust Interstellar Preliminary Examination (ISPE) is a three-year effort to characterize the collection using nondestructive techniques

    Noncoding RNA Mediated Traffic of Foreign mRNA into Chloroplasts Reveals a Novel Signaling Mechanism in Plants

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    Communication between chloroplasts and the nucleus is one of the milestones of the evolution of plants on earth. Proteins encoded by ancestral chloroplast-endogenous genes were transferred to the nucleus during the endosymbiotic evolution and originated this communication, which is mainly dependent on specific transit-peptides. However, the identification of nuclear-encoded proteins targeted to the chloroplast lacking these canonical signals suggests the existence of an alternative cellular pathway tuning this metabolic crosstalk. Non-coding RNAS (NcRNAs) are increasingly recognized as regulators of gene expression as they play roles previously believed to correspond to proteins. Avsunviroidae family viroids are the only noncoding functional RNAs that have been reported to traffic inside the chloroplasts. Elucidating mechanisms used by these pathogens to enter this organelle will unearth novel transport pathways in plant cells. Here we show that a viroid-derived NcRNA acting as a 5′UTR-end mediates the functional import of Green Fluorescent Protein (GFP) mRNA into chloroplast. This claim is supported by the observation at confocal microscopy of a selective accumulation of GFP in the chloroplast of the leaves expressing the chimeric vd-5′UTR/GFP and by the detection of the GFP mRNA in chloroplasts isolated from cells expressing this construct. These results support the existence of an alternative signaling mechanism in plants between the host cell and chloroplasts, where an ncRNA functions as a key regulatory molecule to control the accumulation of nuclear-encoded proteins in this organelle. In addition, our findings provide a conceptual framework to develop new biotechnological tools in systems using plant chloroplast as bioreactors. Finally, viroids of the family Avsunviroidae have probably evolved to subvert this signaling mechanism to regulate their differential traffic into the chloroplast of infected cells

    Status of the Stardust ISPE and the Origin of Four Interstellar Dust Candidates

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    Some bulk properties of interstellar dust are known through infrared and X-ray observations of the interstellar medium. However, the properties of individual interstellar dust particles are largely unconstrained, so it is not known whether individual interstellar dust particles can be definitively distinguished from interplanetary dust particles in the Stardust Interstellar Dust Collector (SIDC) based only on chemical, mineralogical or isotopic analyses. It was therefore understood from the beginning of the Stardust Interstellar Preliminary Examination (ISPE) that identification of interstellar dust candidates would rest on three criteria - broad consistency with known extraterrestrial materials, inconsistency with an origin as secondary ejecta from impacts on the spacecraft, and consistency, in a statistical sense, of observed dynamical properties - that is, trajectory and capture speed - with an origin in the interstellar dust stream. Here we quantitatively test four interstellar dust candidates, reported previously [1], against these criteria

    “Dogged” Search of Fresh Nakhla Surfaces Reveals New Alteration Textures

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    Special Issue: 74th Annual Meeting of the Meteoritical Society, August 8-12, 2011, London, U.K.International audienceCarbonaceous chondrites are considered as amongst the most primitive Solar System samples available. One of their primitive characteristics is their enrichment in volatile elements.This includes hydrogen, which is present in hydrated and hydroxylated minerals. More precisely, the mineralogy is expected to be dominated by phyllosilicates in the case of CM chondrites, and by Montmorillonite type clays in the case of CI. Here, in order to characterize and quantify the abundance of lowtemperature minerals in carbonaceous chondrites, we performed thermogravimetric analysis of matrix fragments of Tagish Lake, Murchison and Orgueil

    Finding Interstellar Particle Impacts on Stardust Aluminium Foils: The Safe Handling, Imaging, and Analysis of Samples Containing Femtogram Residues

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    Impact ionisation detectors on a suite of spacecraft have shown the direction, velocity, flux and mass distribution of smaller ISP entering the Solar System. During the aphelion segments of the Stardust flight, a dedicated collector surface was oriented to intercept ISP of beta = 1, and returned to Earth in January 2006. In this paper we describe the probable appeareance and size of IS particle craters from initial results of experimental impacts and numerical simulation, explain how foils are being prepared and mounted for crater searching by automated acquisition of high magnification electron images (whilst avoiding contamination of the foils) and comment on appropriate analytical techniques for Preliminary Examination (PE)

    Preliminary Examination of the Interstellar Collector of Stardust

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    The findings of the Stardust spacecraft mission returned to earth in January 2006 are discussed. The spacecraft returned two unprecedented and independent extraterrestrial samples: the first sample of a comet and the first samples of contemporary interstellar dust. An important lesson from the cometary Preliminary Examination (PE) was that the Stardust cometary samples in aerogel presented a technical challenge. Captured particles often separate into multiple fragments, intimately mix with aerogel and are typically buried hundreds of microns to millimeters deep in the aerogel collectors. The interstellar dust samples are likely much more challenging since they are expected to be orders of magnitudes smaller in mass, and their fluence is two orders of magnitude smaller than that of the cometary particles. The goal of the Stardust Interstellar Preliminary Examination (ISPE) is to answer several broad questions, including: which features in the interstellar collector aerogel were generated by hypervelocity impact and how much morphological and trajectory information may be gained?; how well resolved are the trajectories of probable interstellar particles from those of interplanetary origin?; and, by comparison to impacts by known particle dimensions in laboratory experiments, what was the mass distribution of the impacting particles? To answer these questions, and others, non-destructive, sequential, non-invasive analyses of interstellar dust candidates extracted from the Stardust interstellar tray will be performed. The total duration of the ISPE will be three years and will differ from the Stardust cometary PE in that data acquisition for the initial characterization stage will be prolonged and will continue simultaneously and parallel with data publications and release of the first samples for further investigation
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