Laboratory study of the impact of repetitive electrical and mechanical stimulation on brown shrimp Crangon crangon

Pulse trawling is currently the best available alternative to beam trawling in the brown shrimp Crangon crangon and Sole Solea solea (also known as Solea vulgaris) fisheries. To evaluate the effect of repetitive exposure to electrical fields, brown shrimp were exposed to the commercial electrodes and pulse settings used to catch brown shrimp (shrimp startle pulse) or Sole (Sole cramp pulse) 20 times in 4 d and monitored for up to 14 d after the first exposure. Survival, egg loss, molting, and the degree of intranuclear bacilliform virus (IBV) infection were evaluated and compared with those in stressed but not electrically exposed (procedural control) and nonstressed, nonexposed (control) brown shrimp as well as brown shrimp exposed to mechanical stimuli. The lowest survival at 14 d (57.3%) occurred in the Sole cramp pulse treatment, and this was significantly lower than in the group with the highest survival, the procedural control (70.3%). No effect of electrical stimulation on the severity of IBV infection was found. The lowest percentage of molts occurred in the repetitive mechanical stimulation treatment (14.0%), and this was significantly lower than in the group with the highest percentage of molts, the procedural control (21.7%). Additionally, the mechanically stimulated brown shrimp that died during the experiment had a significantly larger size than the surviving individuals. Finally, no effect of the shrimp startle pulse was found. Therefore, it can be concluded that repetitive exposure to a cramp stimulus and mechanical stimulation may have negative effects on the growth and/or survival of brown shrimp. However, there is no evidence that electrical stimulation during electrotrawls would have a larger negative impact on brown shrimp stocks than mechanical stimulation during conventional beam trawling

Repositioning the Catalytic Triad Aspartic Acid of Haloalkane Dehalogenase: Effects on Stability, Kinetics, and Structure

Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. The covalent intermediate, which is formed by nucleophilic substitution with Asp124, is hydrolyzed by a water molecule that is activated by His289. The role of Asp260, which is the third member of the catalytic triad, was studied by site-directed mutagenesis. Mutation of Asp260 to asparagine resulted in a catalytically inactive D260N mutant, which demonstrates that the triad acid Asp260 is essential for dehalogenase activity. Furthermore, Asp260 has an important structural role, since the D260N enzyme accumulated mainly in inclusion bodies during expression, and neither substrate nor product could bind in the active-site cavity. Activity for brominated substrates was restored to D260N by replacing Asn148 with an aspartic or glutamic acid. Both double mutants D260N+N148D and D260N+N148E had a 10-fold reduced kcat and 40-fold higher Km values for 1,2-dibromoethane compared to the wild-type enzyme. Pre-steady-state kinetic analysis of the D260N+N148E double mutant showed that the decrease in kcat was mainly caused by a 220-fold reduction of the rate of carbon-bromine bond cleavage and a 10-fold decrease in the rate of hydrolysis of the alkyl-enzyme intermediate. On the other hand, bromide was released 12-fold faster and via a different pathway than in the wild-type enzyme. Molecular modeling of the mutant showed that Glu148 indeed could take over the interaction with His289 and that there was a change in charge distribution in the tunnel region that connects the active site with the solvent. On the basis of primary structure similarity between DhlA and other α/β-hydrolase fold dehalogenases, we propose that a conserved acidic residue at the equivalent position of Asn148 in DhlA is the third catalytic triad residue in the latter enzymes.

Radial velocities of early-type stars in the Perseus OB2 association

We present radial velocities for 29 B- and A-type stars in the field of the nearby association Perseus OB2. The velocities are derived from spectra obtained with AURELIE, via cross correlation with radial velocity standards matched as closely as possible in spectral type. The resulting accuracy is ~2 - 3 km s$^{-1}$. We use these measurements, together with published values for a few other early-type stars, to study membership of the association. The mean radial velocity (and measured velocity dispersion) of Per OB2 is 23.5 \pm 3.9 km s$^{-1}$, and lies ~15 km s$^{-1}$ away from the mean velocity of the local disk field stars. We identify a number of interlopers in the list of possible late-B- and A-type members which was based on Hipparcos parallaxes and proper motions, and discuss the colour-magnitude diagram of the association.Comment: 20 pages, 9 figures, accepted for publication in A&A, minor revision

The role of affective teacher–student relationships in bullying and peer victimization: A multilevel meta-analysis

This meta-analysis synthesizes evidence about the associations of affective teacher–student relationships with bullying perpetration and peer victimization. A systematic database search resulted in 65 primary studies (k) that met the inclusion criteria. The final sample included 185,881 students from preschool to high school. Separate multilevel analyses were conducted for bullying perpetration (k = 25, N = 97,627) and peer victimization (k = 57, N = 151,653). Results showed small to medium, negative overall correlations between teacher-student relationship quality and both bullying perpetration (r = −.17, 95% CI [−.21, −.14]) and peer victimization (r = −.14, 95% CI [−.17, −.11]). Teacher-student relationship quality was also related to less subsequent peer victimization (b = −0.05, 95% CI [−0.08, −0.02]). Associations between teacher-student relationship quality and bullying were stronger for ethnic minority students and when the same informant reported about both variables. Associations with peer victimization were stronger for negative (e.g., conflict) than for positive (e.g., closeness) relationship indicators and when the same informant was used for both variables. Generally, findings demonstrate that higher-quality teacher-student relationships are related to less bullying perpetration and less peer victimization. Hence, promoting positive and minimizing negative teacher-student relationships may help to tackle school-based bullying and peer victimization

Variations in Stellar Clustering with Environment: Dispersed Star Formation and the Origin of Faint Fuzzies

The observed increase in star formation efficiency with average cloud density, from several percent in whole giant molecular clouds to ~30 or more in cluster-forming cores, can be understood as the result of hierarchical cloud structure if there is a characteristic density as which individual stars become well defined. Also in this case, the efficiency of star formation increases with the dispersion of the density probability distribution function (pdf). Models with log-normal pdf's illustrate these effects. The difference between star formation in bound clusters and star formation in loose groupings is attributed to a difference in cloud pressure, with higher pressures forming more tightly bound clusters. This correlation accounts for the observed increase in clustering fraction with star formation rate and with the observation of Scaled OB Associations in low pressure environments. ``Faint fuzzie'' star clusters, which are bound but have low densities, can form in regions with high Mach numbers and low background tidal forces. The proposal by Burkert, Brodie & Larsen (2005) that faint fuzzies form at large radii in galactic collisional rings, satisfies these constraints.Comment: 14 pages, 2 figures, ApJ, 672, January 10th 200