158 research outputs found
Effects of tallow and ground flaxseed on sensory and color characteristics of ribeye steaks
Forty-eight ribeye steaks from steers
fed diets containing steam-flaked corn
(SFC), steam-flaked corn with tallow
(SFC/Tallow), or steam-flaked corn with
rolled flaxseed (Flax) were used to evaluate
the effects of dietary fat on sensory traits,
retail display color stability, and fatty acid
composition. Steaks from Flax-fed steers
had increased deposition of alpha linolenic
acid (C18:3n3, an omega-3 fatty acid;
P<0.01) and developed a detectable off-flavor
(P<0.05) when compared to those of
cattle fed SFC and SFC/Tallow. There
were no differences in tenderness,
juiciness, or flavor intensity (P>0.10)
among the three treatments. Steaks from
cattle fed SFC retained a desirable color
longer than those from cattle fed Flax
(P<0.05) which may be attributable to
premature lipid oxidation in steaks from
cattle fed Flax. This study suggests that
altering the fat in the diet may affect flavor
and color stability of the meat. Feeding
flaxseed can effectively alter composition
of carcass tissues to yield beef that is high
in omega-3 fatty acids, which may lead to
premature lipid oxidation
Induced Anisotropies in NiCo Obliquely Deposited Films and Their effect on Magnetic Domains
English Article: Oblique and in-plane anisotropies in obliquely evaporated NiCo thin films were investigated in order to understand their origin. All the compositions studied clearly show the effect of columnar grain morphology coupled with some intrinsic factors such as magnetostriction and crystallinity. Energy calculations are undertaken to explain the effect of
Effects of supplementation of limit-fed growing diets with either soybean meal or nonenzymatically browned soybean meal on steer performance
Seventy two individually fed Angus × Hereford
steers (642 lb) were used to evaluate the
effects of supplementing limit-fed, growing diets
with either soybean meal (SBM) or nonenzymatically
browned soybean meal (NSBM).
Eight steers were allotted to a control diet
composed of 39.1% high-moisture corn, 42%
cottonseed hulls, 10.4% ground corn, 5% cane
molasses 2.25% urea, and 1.5% vitamins and
minerals (dry basis). The remaining steers were
allotted to diets that derived 100, 80, 60, or
40% of their supplemental protein from SBM or
60, 45, 30, or 15% of their supplemental protein
from NSBN. The balance of supplemental
protein came from urea. All diets were formulated
to contain 13.0% crude protein (dry
basis). Steers were fed once daily for 80 days
at 2.25% of BW. Average daily gain and
efficiency did not differ (P>.05) between
sources (ADG=1.932 + .103 × (% CP from
SBM) + .097 × (% CP from NSBM);
gain:feed=.140 + .0058 × (% CP from SBM) +
.0051 × (% CP from NSBM)). The lack of
response to NBSBM supplementation above
that for SBM suggests that either degradable
intake protein was limiting in the basal diet or a
large proportion of the amino acids in the
NSBM were unavailable due to overprocessing
Temperature-dependent interface magnetism and magnetization reversal in Co/Pt multilayers
We report on the temperature dependence of the magnetic properties and interface magnetism of Co/Pt multilayers. The magnetic properties including magnetization and anisotropy change substantially as the temperature varies from 300 to 10 K for samples with Co layer thickness in the range from 3 to 7 Å. The interface anisotropy of about 0.38 erg/cm2 is nearly independent of temperature. The magnetization reversal is dominated by domain wall motion for the thinner Co layers and dominated by nucleation for the thicker Co layers
A potential role for muscle in glucose homeostasis: in vivo kinetic studies in glycogen storage disease type 1a and fructose-1,6-bisphosphatase deficiency
A potential role for muscle in glucose homeostasis was recently suggested based on characterization of extrahepatic and extrarenal glucose-6-phosphatase (glucose-6-phosphatase-beta). To study the role of extrahepatic tissue in glucose homeostasis during fasting glucose kinetics were studied in two patients with a deficient hepatic and renal glycogenolysis and/or gluconeogenesis. Endogenous glucose production (EGP), glycogenolysis (GGL), and gluconeogenesis (GNG) were quantified with stable isotopes in a patient with glycogen storage disease type 1a (GSD-1a) and a patient with fructose-1,6-bisphosphatase (FBPase) deficiency. The [6,6-H-2(2)]glucose dilution method in combination with the deuterated water method was used during individualized fasting tests. Both patients became hypoglycemic after 2.5 and 14.5 h fasting, respectively. At that time, the patient with GSD-1a had EGP 3.84 mu mol/kg per min (30% of normal EGP after an overnight fast), GGL 3.09 mu mol/kg per min, and GNG 0.75 mu mol/kg per min. The patient with FBPase deficiency had EGP 8.53 mu mol/kg per min (62% of normal EGP after an overnight fast), GGL 6.89 mu mol/kg per min GGL, and GNG 1.64 mu mol/kg per min. EGP was severely hampered in both patients, resulting in hypoglycemia. However, despite defective hepatic and renal GNG in both disorders and defective hepatic GGL in GSD-1a, both patients were still able to produce glucose via both pathways. As all necessary enzymes of these pathways have now been functionally detected in muscle, a contribution of muscle to EGP during fasting via both GGL as well as GNG is suggeste
Novel compound heterozygous STN1 variants are associated with Coats Plus syndrome
Funder: WellcomeAbstract: Aim: Coats plus syndrome (CP) is a rare autosomal recessive disorder, characterised by retinal telangiectasia exudates (Coats disease), leukodystrophy, distinctive intracranial calcification and cysts, as well as extra‐neurological features including abnormal vasculature of the gastrointestinal tract, portal hypertension and osteopenia with a tendency to fractures. CP most frequently occurs due to loss‐of‐function mutations in CTC1. The encoded protein CTC1 constitutes part of the CST (CTC1‐STN1‐TEN1) complex, and three patients have been described with CP due to biallelic mutations in STN1. Together with the identification of homozygosity for a specific loss‐of‐function mutation in POT1 in a sibling pair, these observations highlight a defect in the maintenance of telomere integrity as the cause of CP, although the precise mechanism leading to the micro‐vasculopathy seen at a pathological level remains unclear. Here, we present the investigation of a fourth child who presented to us with retinal exudates, intracranial calcifications and developmental delay, in keeping with a diagnosis of CP, and later went on to develop pancytopenia and gastrointestinal bleeding. Genome sequencing revealed compound heterozygous variants in STN1 as the likely genetic cause of CP in this present case. Methods: We assessed the phenotype to be CP and undertook targeted sequencing. Results: Whilst sequencing of CTC1 and POT1 was normal, we identified novel compound heterozygous variants in STN1 (previous gene symbol OBFC1): one loss‐of‐function––c.894dup (p.(Asp299Argfs*58)); and one missense––c.707T>C (p.(Leu236Pro)). Conclusion: Given the clinical phenotype and identified variants we suggest that this is only the fourth patient reported to date with CP due to mutations in STN1
Clearance of technetium-99m-DTPA and HRCT findings in the evaluation of patients with Idiopathic Pulmonary Fibrosis
BACKGROUND: Clearance of inhaled technetium-labeled diethylenetriamine pentaacetate ((99m)Tc-DTPA) is a marker of epithelial damage and an index of lung epithelial permeability. The aim of this study was to investigate the role of (99m)Tc-DTPA scan in patients with Idiopathic Pulmonary Fibrosis (IPF). Our hypothesis is that the rate of pulmonary (99m)Tc-DTPA clearance could be associated with extent of High Resolution Computed Tomography (HRCT) abnormalities, cell differential of bronchoalveolar lavage fluid (BALF) and pulmonary function tests (PFTs) in patients with IPF. METHODS: We studied prospectively 18 patients (14 male, 4 female) of median age 67yr (range 55–81) with histologically proven IPF. HRCT scoring included the mean values of extent of disease. Mean values of these percentages represented the Total Interstitial Disease Score (TID). DTPA clearance was analyzed according to a dynamic study using a Venticis II radioaerosol delivery system. RESULTS: The mean (SD) TID score was 36 ± 12%, 3 patients had mild, 11 moderate and 4 severe TID. Abnormal DTPA clearance half-time (t(1/2)<40 min) was found in 17/18 (94.5%) [mean (SD) 29.1 ± 8.6 min]. TID was weakly correlated with the DTPA clearance (r = -0.47, p = 0.048) and with % eosinophils (r = 0.475, p = 0.05). No correlation was found between TID score or DTPA and PFTs in IPF patients. CONCLUSION: Our data suggest that (99m)Tc-DTPA lung scan is not well associated with HRCT abnormalities, PFTs, and BALF cellularity in patients with IPF. Further studies in large scale of patients are needed to define the role of this technique in pulmonary fibrosis
The Biological Geography of the European Seas: results from the macrofaunal inventory of the soft-substrate communities
The natural history of classic galactosemia: lessons from the GalNet registry.
BACKGROUND
Classic galactosemia is a rare inborn error of carbohydrate metabolism, caused by a severe deficiency of the enzyme galactose-1-phosphate uridylyltransferase (GALT). A galactose-restricted diet has proven to be very effective to treat the neonatal life-threatening manifestations and has been the cornerstone of treatment for this severe disease. However, burdensome complications occur despite a lifelong diet. For rare diseases, a patient disease specific registry is fundamental to monitor the lifespan pathology and to evaluate the safety and efficacy of potential therapies. In 2014, the international Galactosemias Network (GalNet) developed a web-based patient registry for this disease, the GalNet Registry. The aim was to delineate the natural history of classic galactosemia based on a large dataset of patients.
METHODS
Observational data derived from 15 countries and 32 centers including 509 patients were acquired between December 2014 and July 2018.
RESULTS
Most affected patients experienced neonatal manifestations (79.8%) and despite following a diet developed brain impairments (85.0%), primary ovarian insufficiency (79.7%) and a diminished bone mineral density (26.5%). Newborn screening, age at onset of dietary treatment, strictness of the galactose-restricted diet, p.Gln188Arg mutation and GALT enzyme activity influenced the clinical picture. Detection by newborn screening and commencement of diet in the first week of life were associated with a more favorable outcome. A homozygous p.Gln188Arg mutation, GALT enzyme activity of ≤ 1% and strict galactose restriction were associated with a less favorable outcome.
CONCLUSION
This study describes the natural history of classic galactosemia based on the hitherto largest data set
Induced anisotropies in NiCo obliquely deposited films and their effect on magnetic domains
- …