Continuous light increases growth, daily carbon gain, antioxidants, and alters carbohydrate metabolism in a cultivated and a wild tomato species

Cultivated tomato species develop leaf injury while grown in continuous light (CL). Growth, photosynthesis, carbohydrate metabolism and antioxidative enzyme activities of a cultivated (Solanum lycopersicum L. ‘Aromata’) and a wild tomato species (Solanum pimpinellifolium L.) were compared in this study aiming to analyse the species-specific differences and thermoperiod effects in responses to CL. The species were subjected to three photoperiodic treatments for 12 days in climate chambers: 16-h photoperiod with a light/dark temperature of 26/16ºC (P16D10 or control); CL with a constant temperature of 23ºC (P24D0); CL with a variable temperature of 26/16ºC (P24D10). The results showed that both species grown in CL had higher dry matter production due to the continuous photosynthesis and a subsequent increase in carbon gain. In S. lycopersicum, the rate of photosynthesis and the maximum photochemical efficiency of photosystem II declined in CL with the development of leaf chlorosis, reduction in the leaf chlorophyll content and a higher activity of antioxidative enzymes. The normal diurnal patterns of starch and sugar were only present under control conditions. The results demonstrated that CL conditions mainly affected the photosynthetic apparatus of a cultivated species (S. lycopersicum), and to a less degree to the wild species (S. pimpinellifolium). The negative effects of the CL could be alleviated by diurnal temperature variations, but the physiological mechanisms behind these are less clear. The results also show that the genetic potential for reducing the negative effects of CL does exist in the tomato germplasm

What lies behind a no? Coercion in dementia care

Bacheloroppgave sykepleie, 2014Tittel; Hva ser du søster, bak et nei? - Tvang i demensomsorgen. Engelsk tittel; What lies behind a no? - Coercion in dementia care. Formålet med denne bacheloroppgaven er å finne ut hvordan sykepleiere kan redusere bruk av tvang i demensomsorgen. Problemstillingen er som følger; Hvordan kan sykepleiere begrense bruk av tvang i møte med pasienter med demens, som motsetter seg hjelp til å ivareta personlig hygiene. Problemstillingen begrenser derfor oppgaven til å handle om tvang i forbindelse med personlig hygiene. Vi har skrevet en case som beskriver det vi anser som en typisk pasientsituasjon som lett kan gå over i tvang. Oppgaven er en litteraturstudie som er basert på anerkjente forskere sine studier, pensumlitteratur og selvvalgt litteratur. Funnene blir drøftet og satt opp mot hverandre, for å finne en best mulig løsning for pasienten i casen. Resultatene vi har kommet frem til at tid og kunnskap er utslagsgivende for å begrense bruken av tvang

Abdominal examination during pregnancy may enhance relationships between midwife, mother and child : a qualitative study of pregnant women's experiences

Background: Abdominal examination is a routine procedure performed by midwives several times during pregnancy to monitor the growth and well-being of the baby. Literature and instructions regarding abdominal examination focus on the technical performance, with limited attention paid to the women’s experience of the examination or the bonding-related aspects between the mother and baby. The aim of the study was to explore how pregnant women experience the abdominal examination and how the examination affects maternal–fetal attachment. Methods: Participant observation and semi-structured interviews with 10 pregnant women. We used thematic analysis to identify themes across the empirical material. Results: We identified the following four central themes: an essential examination, the baby becomes real, the importance of being involved and different senses provide different experiences. These themes describe how the women regarded the abdominal examination as an essential part of the midwifery consultation and considered it the occasion when the baby became real and tangible. Being prepared and involved before and during the examination were pivotal for how the examination was experienced by the women. The abdominal examination was crucial to the pregnant women because it provided them with important sensory aspects that were not obtained from ultrasound examination. Conclusion: The abdominal examination is regarded as essential in midwifery consultations and has the potential for supporting a woman’s bodily sensation of her baby, which is reinforced by the midwife’s manual palpation. Touch can be a way for a pregnant woman to become acquainted with her unborn child, which provides midwives a profound potential to facilitate the process of maternal–fetal attachment

Initial experiments to assess short-term survival of discarded plaice (Pleuronectes platessa) caught in trammel nets during winter season

European plaice (Pleuronectes platessa) is a key species in commercial fisheries in the North Sea, Skagerrak, Kattegat, and Baltic Sea. The reformed European Union Common Fisheries Policy includes the possibility of exemptions from the landing obligation for “species for which scientific evidence demonstrates high survival rates”. Discard survival from set-net fisheries is poorly studied. Trials were conducted on two commercial fishing vessels over seven trips from November to February 2017–2018 in the Baltic Sea. The nylon trammel nets had a nominal bar size for the inner/outer wall of 75/350 mm and 85/400 mm. Soaking time was 23–47 h, water depth 7–18 m, and deck temperature was − 0.1–6.0 ◦C. Following commercial practice, the trammel nets were hauled back onto the vessel, after which netting and fish passed through a net hauler onto a steel sorting table where the entire fish catch were manually untangled by the fishers and plaice collected by scientists. We used a storage system for housing the captured fish inside fishing harbours during observations. Catch-damage-index (CDI) and Reflex Action Mortality Predictor (RAMP) scores were used to assess fish condition immediately after capture and at the end of observation periods. All plaice below 40 cm were sampled with a total number of 118 individuals from 13 fleets (several nets joined together). The fish were assessed for short-term survival for 4–10 days with full survival (100%). The majority of fish exhibited no reflex impairments. Minor bruises, fraying, and net marks were frequently observed on captured fish. The overall condition of the fish did not change during observation periods

Stable isotope ratios of nitrogen and carbon as biomarkers of a vegan diet

Purpose Dietary biomarkers can potentially overcome the limitations of self-reported dietary data. While in ecology and archaeology, stable isotope ratios of carbon and nitrogen are widely used as biomarkers, this is not the case in nutrition research. Since the abundance of the 13C and the 15N isotope differ in food sources from plant and animal origin, stable isotope ratios of carbon and nitrogen (δ13C and δ15N) may differ in human biological material. Here, we investigated the stable isotope ratios of nitrogen and carbon in serum and urine from vegans and omnivores. Method Measurement of δ15N and δ13C in serum and 24 h urine was performed by Elemental Analyzer–Isotope Ratio Mass Spectrometer in the cross-sectional study “Risks and Benefits of a Vegan Diet”. The study included 36 vegans and 36 omnivores with a median age of 37.5 years (matched for age and sex), who adhered to their diet for at least 1 year. Results Both δ15N and δ13C were significantly lower in both the serum and 24 h urine of vegans compared to omnivores. δ15N either in serum or urine had 100% specificity and sensitivity to discriminate between vegans and omnivores. Specificity of δ13C was also > 90%, while sensitivity was 93% in serum and 77% in urine. Conclusion δ15N both in serum and urine was able to accurately identify vegans and thus appears to be a promising marker for dietary habits.publishedVersio

Structure, dynamics, and stability of the globular domain of human linker histone H1.0 and the role of positive charges

Linker histone H1 (H1) is an abundant chromatin-binding protein that acts as an epigenetic regulator binding to nucleosomes and altering chromatin structures and dynamics. Nonetheless, the mechanistic details of its function remain poorly understood. Recent work suggest that the number and position of charged side chains on the globular domain (GD) of H1 influence chromatin structure and hence gene repression. Here, we solved the solution structure of the unbound GD of human H1.0, revealing that the structure is almost completely unperturbed by complex formation, except for a loop connecting two antiparallel β-strands. We further quantified the role of the many positive charges of the GD for its structure and conformational stability through the analysis of 11 charge variants. We find that modulating the number of charges has little effect on the structure, but the stability is affected, resulting in a difference in melting temperature of 26 K between GD of net charge +5 versus +13. This result suggests that the large number of positive charges on H1-GDs have evolved for function rather than structure and high stability. The stabilization of the GD upon binding to DNA can thus be expected to have a pronounced electrostatic component, a contribution that is amenable to modulation by posttranslational modifications, especially acetylation and phosphorylation. Keywords: CD; NMR; histone; nucleosome; protein electrostatics; protein stability; protein structur