Compensation grouting to control deep excavation ground movements

The research conducted concerns the application of compensation grouting, particularly compaction grouting, as a method to reduce and control the ground movements generated in the soil by a deep retained excavation in firm to stiff clays. Compaction grouting involves the injection of grout into the soil to create a spherical or cylindrical grout bulb. This research investigates the effectiveness and limitations of the method to provide a preliminary understanding of the influence of the grouting volume, timing and position on the vertical settlements at the retained surface and horizontal displacements of the wall relative to the behaviour observed from the corresponding scenario in which only excavation occurs. Experimental data were obtained from a series of 10 successful centrifuge model tests undertaken at 100 g. The plane strain models consisted of a pre-formed 12 m deep (at prototype scale) retained excavation temporarily supported by the pressure of a dense fluid acting against the wall and formation surface and a relatively flexible retaining wall propped at the top. The dense fluid was removed and the subsequent soil movements at the retained surface, wall and the formation level were measured, using a combination of transducers and analysis of digital images taken of targets embedded in the front face of the model and wall. Three reference tests were conducted to establish the magnitude and pattern of soil and wall displacements generated by excavation alone. Idealised compaction grouting was modelled simultaneously with excavation in the remaining tests with the injection of ‘grout’ (water) into sealed latex tubes (supported by a perforated Nylon tube), inserted in the soil behind the excavation. The start of injection relative to the timing of the excavation varied amongst the tests. Grouting was continued until positive compensation of the local surface settlement was noted or significant horizontal displacements of the wall were observed. The tests showed that there appears to be no distinct relationship between the grout volume and the displacements at the retained surface above the injection and at a wall depth of 0.75 times the excavation depth, H due to injection. However, it was seen that for injections below a depth of 0.25H different critical volumes existed beyond which a positive compensation of the retained surface deteriorated or negative compensation increased at a greater rate. This was also reflected in the wall behaviour. The different injection initiation times showed that greater positive compensation effects could be achieved with injections up to a depth of 0.5H when conducted during the excavation, rather than in the period after. Timing had no influence on injections below this depth. The influence of injection timing was found to be secondary to the injection position. A linear relationship between the depth of injection and either positively or negatively compensated settlements was noted from the tests. Positive compensation of the ground surface is possible for injections conducted above a depth of 0.5H. However, below this depth a significant negative compensation effect on surface settlements and horizontal wall movements was noted regardless of timing or volume. Greater positive compensation effects and reduced negative effects on the wall were noted with increasing distance from the wall. Regions behind the wall have been identified where grouting provides positive compensation of the surface with minimal influence on the wall and where only negative effects are observed at both the surface and wall

Plate bearing tests for working platforms

During piling and other construction works, a working platform is often constructed across the site. These platforms comprise aggregate material placed and compacted to a designed thickness. Satisfactory performance of the platform may be confirmed by a plate bearing test. Current guidance given on plate bearing testing of granular soils suggests that the plate be at least five times the nominal size of the coarsest material. For a working platform this may be large and the reaction load required from plant and resources to carry out the bearing test may become excessively high. The aim of the research presented in this paper was to investigate the effect of particle to plate size ratios to establish if the use of a smaller plate would still allow a reliable test to be performed on site. Plate bearing tests were carried out in a centrifuge using a large, coarse grained limestone. The limestone was graded to a scale representation of 6F2 material, a commonly specified particle size distribution for working platforms. The size of plate was varied and the load displacement response recorded. The measured bearing capacity was correlated with the ratio of particle to plate size

Centrifuge modelling to determine the influence of pile stiffness on pile capacity

The stiffness of piles relates to their ability to resist deformation in response to an applied force. The modulus of elasticity of an uncracked concrete piles typically varies between 30-40kN/m2. Under axial loading these high stiffness piles transfer the load through the pile to the base. This results in low mobilisation of shaft friction as the stiff pile displaces uniformly and therefore the magnitude of skin friction along the entire length of the pile is small. In addition, base resistance of deep piles is mobilised at very high loads which may exceed the working load of the pile shaft. The adhesion factor, α, for bored piles in London Clay can range between 0.45 and 0.6 suggesting that a significant proportion of the soil strength cannot be mobilised. This low mobilisation of shaft resistance means that the ultimate bearing capacity is much reduced. The research investigated the behaviour of a low stiffness pile under axial load and compared this with a conventional high stiffness pile. The results demonstrated that the low stiffness pile exhibited marginally greater capacity at working load and a noticeably improved capacity at ultimate load

In silico and in vitro analysis of lncRNA XIST reveals a panel of possible lung cancer regulators and a five-gene diagnostic signature

© 2020 by the authors. Long non-coding RNAs (lncRNAs) perform a wide functional repertoire of roles in cell biology, ranging from RNA editing to gene regulation, as well as tumour genesis and tumour progression. The lncRNA X-inactive specific transcript (XIST) is involved in the aetiopathogenesis of non-small cell lung cancer (NSCLC). However, its role at the molecular level is not fully elucidated. The expression of XIST and co-regulated genes TSIX, hnRNPu, Bcl-2, and BRCA1 analyses in lung cancer (LC) and controls were performed in silico. Differentially expressed genes (DEGs) were determined using RNA-seq in H1975 and A549 NSCLC cell lines following siRNA for XIST. XIST exhibited sexual dimorphism, being up-regulated in females compared to males in both control and LC patient cohorts. RNA-seq revealed 944 and 751 DEGs for A549 and H1975 cell lines, respectively. These DEGs are involved in signal transduction, cell communication, energy pathways, and nucleic acid metabolism. XIST expression associated with TSIX, hnRNPu, Bcl-2, and BRCA1 provided a strong collective feature to discriminate between controls and LC, implying a diagnostic potential. There is a much more complex role for XIST in lung cancer. Further studies should concentrate on sex-specific changes and investigate the signalling pathways of the DEGs following silencing of this lncRNA

Allele-Specific Small Interfering RNA Corrects Aberrant Cellular Phenotype in Keratitis-Ichthyosis-Deafness Syndrome Keratinocytes.

Keratitis-ichthyosis-deafness (KID) syndrome is a severe, untreatable condition characterized by ocular, auditory, and cutaneous abnormalities, with major complications of infection and skin cancer. Most cases of KID syndrome (86%) are caused by a heterozygous missense mutation (c.148G>A, p.D50N) in the GJB2 gene, encoding gap junction protein Cx26, which alters gating properties of Cx26 channels in a dominant manner. We hypothesized that a mutant allele-specific small interfering RNA could rescue the cellular phenotype in patient keratinocytes (KCs). A KID syndrome cell line (KID-KC) was established from primary patient KCs with a heterozygous p.D50N mutation. This cell line displayed impaired gap junction communication and hyperactive hemichannels, confirmed by dye transfer, patch clamp, and neurobiotin uptake assays. A human-murine chimeric skin graft model constructed with KID-KCs mimicked patient skin in vivo, further confirming the validity of these cells as a model. In vitro treatment with allele-specific small interfering RNA led to robust inhibition of the mutant GJB2 allele without altering expression of the wild-type allele. This corrected both gap junction and hemichannel activity. Notably, allele-specific small interfering RNA treatment caused only low-level off-target effects in KID-KCs, as detected by genome-wide RNA sequencing. Our data provide an important proof-of-concept and model system for the potential use of allele-specific small interfering RNA in treating KID syndrome and other dominant genetic conditions

The London Geotechnical Centrifuge Centre at City University London

The London Geotechnical Centrifuge Centre located at City University, London is one of four currently active centrifuges in the UK. The centrifuge is well used and much of the geotechnical research at City University employs physical modelling but supported by a large element testing facility and numerical modelling capability. The centre was established in 1990 and the facility was extensively upgraded to provide more space for sample preparation and model making in 2004. In 2012 the centre is supporting 4 doctoral research projects in addition to visitors from China, Italy and UK. The focus of research is urban construction processes with an underlying theme of sustainability. Over the last 10 years the group has established a capability for tackling complex modelling problems and is currently investigating the application of smart instrumentation and control at high g

Ethics-in-practice in fragile contexts: research in education for displaced persons, refugees and asylum seekers

The rising numbers of forcibly displaced peoples on the move globally, and the challenges with providing access to education, reflects the shifting and complex times that we live in. Even though there has been a proliferation in educational research in the context of forced migration, in line with the increasing number of forced migrants, there has not been a commensurate focus on unpicking the increasingly complex ethical conditions within which researchers and participants operate. To examine this issue, the article provides three narrated accounts by researchers in this field and explores the interaction of researcher and researcher-author voice to critically appraise their research experience and identify critical reflections of understanding of ethics-in-practice in fragile contexts. These narratives are framed by the CERD ethical appraisal framework which explores ethical thinking through four ethical lenses – Consequential, Ecological, Relational and Deontological. The article contributes to a deeper understanding of ethics-in-practice as a central dimension in educational research. The implications of this work show how one-size-fits-all approach to ethical appraisal is inappropriate for a socially just educational research. This work also illustrates the importance of attending to relationships and voice of the forcibly displaced, both of which are often lacking in educational research in fragile contexts

Not Managing Expectations: A Grounded Theory of Intimate Partner Violence From the Perspective of Pakistani People

Intimate partner violence (IPV) is a major social and public health problem affecting people from different cultures and societies. Much research has been undertaken to understand the phenomenon, its determinants, and its consequences in numerous countries. However, there is a paucity of research on IPV in many areas of the world including Pakistan. The present study aimed to develop a theory of the meaning and process of IPV from the perspective of Pakistani men and women living in and outside Pakistan

Population-scale proteome variation in human induced pluripotent stem cells

Human disease phenotypes are driven primarily by alterations in protein expression and/or function. To date, relatively little is known about the variability of the human proteome in populations and how this relates to variability in mRNA expression and to disease loci. Here, we present the first comprehensive proteomic analysis of human induced pluripotent stem cells (iPSC), a key cell type for disease modelling, analysing 202 iPSC lines derived from 151 donors, with integrated transcriptome and genomic sequence data from the same lines. We characterised the major genetic and non-genetic determinants of proteome variation across iPSC lines and assessed key regulatory mechanisms affecting variation in protein abundance. We identified 654 protein quantitative trait loci (pQTLs) in iPSCs, including disease-linked variants in protein-coding sequences and variants with trans regulatory effects. These include pQTL linked to GWAS variants that cannot be detected at the mRNA level, highlighting the utility of dissecting pQTL at peptide level resolution

Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone

It is recognized that some mutated cancer genes contribute to the development of many cancer types, whereas others are cancer type specific. For genes that are mutated in multiple cancer classes, mutations are usually similar in the different affected cancer types. Here, however, we report exquisite tumor type specificity for different histone H3.3 driver alterations. In 73 of 77 cases of chondroblastoma (95%), we found p.Lys36Met alterations predominantly encoded in H3F3B, which is one of two genes for histone H3.3. In contrast, in 92% (49/53) of giant cell tumors of bone, we found histone H3.3 alterations exclusively in H3F3A, leading to p.Gly34Trp or, in one case, p.Gly34Leu alterations. The mutations were restricted to the stromal cell population and were not detected in osteoclasts or their precursors. In the context of previously reported H3F3A mutations encoding p.Lys27Met and p.Gly34Arg or p.Gly34Val alterations in childhood brain tumors, a remarkable picture of tumor type specificity for histone H3.3 driver alterations emerges, indicating that histone H3.3 residues, mutations and genes have distinct functions