Variation in Microbial Community Composition and Culturability in the Rhizosphere of Leucanthemopsis alpina (L.) Heywood and Adjacent Bare Soil Along an Alpine Chronosequence

We compared the size, culturability, diversity, and dominant species similarity of the bacterial communities of Leucanthemopsis alpina (L.) Heywood rhizosphere and adjacent bare soil (interspace) along a chronosequence of soil development time (5, 50, and 70years) in the forefield of the Dammaglacier (Switzerland). We found no evidence that the size of the bacterial community was significantly affected by either soil age or the presence of L. alpina. In contrast, the proportion of the bacterial community that could be cultured on nonselective agars, and which was taken as an indication of the proportion of r-selected populations, was significantly higher in the 50- and 70-year-old soils than in the 5-year-old soil, and was also significantly higher in the rhizosphere of L. alpina at all time points. RDA indicated significant correlations between the increased culturability of the bacterial community over time and increasing concentrations of labile N, and between the increased culturability in the rhizosphere and increased concentrations of labile C and N. HaeIII-amplified ribosomal DNA (rDNA) restriction analysis of a library of 120 clones of 16S rDNA revealed 85 distinct phylotypes. Hurlbert's probability of interspecific encounter (PIE) values derived from this library ranged from 0.95 to 1.0, indicating a very high genetic diversity. There was no significant difference in the PIE values of rhizosphere and interspace communities. Detrended correspondence analysis (DCA) of 16S ribosomal RNA (rRNA) denaturing gradient gel electrophoresis (DGGE) community profiles clearly distinguished the rhizosphere from the interspace community in the 5-year-old soils and also clearly distinguished between these communities and the rhizosphere and interspace communities of the 50- and 70-year-old soils. However, 16S rRNA DGGE revealed little difference between rhizosphere and interspace communities in the 50- and 70-year-old soils. The relative similarity of the 16S rRNA profiles strongly reflected labile carbon and nitrogen availability. Overall, our results suggest that improved C and N availability in the rhizosphere of L. alpina increases the size of r-selected bacterial species populations, but that the influence of L. alpina depends on soil age, being maximal in the youngest soils and minimal in the oldest. The reduced influence of L. alpina in the older soils may reflect a feedback between improved nutrient availability and reduced rhizodepositio

Potential and limits of InSAR to characterize interseismic deformation independently of GPS data: Application to the southern San Andreas Fault system

The evaluation of long-wavelength deformation associated with interseismic strain accumulation traditionally relies on spatially sparse GPS measurements, or on high spatial-resolution InSAR velocity fields aligned to a GPS-based model. In this approach the InSAR contributes only short-wavelength deformation and the two data sets are dependent, thereby challenging the evaluation of the InSAR uncertainties and the justification of atmospheric corrections. Here we present an analysis using 7 years of Envisat InSAR data to characterize interseismic deformation along the southern San Andreas Fault (SAF) and the San Jacinto Fault (SJF) in southern California, where the SAF bifurcates onto the Mission Creek (MCF) and the Banning (BF) fault strands. We outline the processing steps for using InSAR alone to characterize both the short- and long-wavelength deformation, and evaluate the velocity field uncertainties with independent continuous GPS data. InSAR line-of-sight (LOS) and continuous GPS velocities agree within ∼1–2 mm/yr in the study area, suggesting that multiyear InSAR time series can be used to characterize interseismic deformation with a higher spatial resolution than GPS. We investigate with dislocation models the ability of this mean LOS velocity field to constrain fault slip rates and show that a single viewing geometry can help distinguish between different slip-rate scenarios on the SAF and SJF (∼35 km apart) but multiple viewing geometries are needed to differentiate slip on the MCF and BF (<12 km apart). Our results demonstrate that interseismic models of strain accumulation used for seismic hazards assessment would benefit from the consideration of InSAR mean velocity maps

Interseismic coupling and refined earthquake potential on the Hayward-Calaveras fault zone

Interseismic strain accumulation and fault creep is usually estimated from GPS and alignment arrays data, which provide precise but spatially sparse measurements. Here we use interferometric synthetic aperture radar to resolve the interseismic deformation associated with the Hayward and Calaveras Faults (HF and CF) in the East San Francisco Bay Area. The large 1992–2011 SAR data set permits evaluation of short- and long-wavelength deformation larger than 2 mm/yr without alignment of the velocity field to a GPS-based model. Our time series approach in which the interferogram selection is based on the spatial coherence enables deformation mapping in vegetated areas and leads to refined estimates of along-fault surface creep rates. Creep rates vary from 0 ± 2 mm/yr on the northern CF to 14 ± 2 mm/yr on the central CF south of the HF surface junction. We estimate the long-term slip rates by inverting the long-wavelength deformation and the distribution of shallow slip due to creep by inverting the remaining velocity field. This distribution of slip reveals the locations of locked and slowly creeping patches with potential for a M6.8 ± 0.3 on the HF near San Leandro, a M6.6 ± 0.2 on the northern CF near Dublin, a M6.5 ± 0.1 on the HF south of Fremont, and a M6.2 ± 0.2 on the central CF near Morgan Hill. With cascading multisegment ruptures the HF rupturing from Berkeley to the CF junction could produce a M6.9 ± 0.1, the northern CF a M6.6 ± 0.1, the central CF a M6.9 ± 0.2 from the junction to Gilroy, and a joint rupture of the HF and central CF could produce a M7.1 ± 0.1

Dissemination of antibiotic resistance genes associated with the sporobiota in sediments impacted by wastewater.

Aquatic ecosystems serve as a dissemination pathway and a reservoir of both antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG). In this study, we investigate the role of the bacterial sporobiota to act as a vector for ARG dispersal in aquatic ecosystems. The sporobiota was operationally defined as the resilient fraction of the bacterial community withstanding a harsh extraction treatment eliminating the easily lysed fraction of the total bacterial community. The sporobiota has been identified as a critical component of the human microbiome, and therefore potentially a key element in the dissemination of ARG in human-impacted environments. A region of Lake Geneva in which the accumulation of ARG in the sediments has been previously linked to the deposition of treated wastewater was selected to investigate the dissemination of &lt;i&gt;tet&lt;/i&gt; (W) and &lt;i&gt;sul&lt;/i&gt; 1, two genes conferring resistance to tetracycline and sulfonamide, respectively. Analysis of the abundance of these ARG within the sporobiome (collection of genes of the sporobiota) and correlation with community composition and environmental parameters demonstrated that ARG can spread across the environment with the sporobiota being the dispersal vector. A highly abundant OTU affiliated with the genus &lt;i&gt;Clostridium&lt;/i&gt; was identified as a potential specific vector for the dissemination of &lt;i&gt;tet&lt;/i&gt; (W), due to a strong correlation with &lt;i&gt;tet&lt;/i&gt; (W) frequency (ARG copy numbers/ng DNA). The high dispersal rate, long-term survival, and potential reactivation of the sporobiota constitute a serious concern in terms of dissemination and persistence of ARG in the environment

Interseismic quiescence and triggered slip of active normal faults of Kīlauea Volcano's south flank during 2001-2018

The mobile south flank of Kīlauea Volcano hosts two normal fault systems, the Koa'e fault system (KFS) and the Hilina fault system (HFS). In historical time, at least three M>6.5 earthquakes have occurred on the basal detachment of the Kīlauea Volcano's south flank, with the most recent being the 4 May 2018 M6.9 earthquake. Here we analyze kinematic Global Positioning System data collected from 2001 to 2017 and interferometric synthetic aperture radar data before, during, and after the 2018 M6.9 earthquake to determine the crustal motion across the HFS and KFS faults. Our results indicate that the HFS faults did not significantly slip during the interseismic period from 2007 to 2011. Despite its substantial magnitude, interferometric synthetic aperture radar (InSAR) data show that the 2018 M6.9 earthquake triggered subcentimeter level slip along sections of the previously mapped HFS branches. Up to 20 cm of offset occurred on what appears to be a newly formed (or previously unknown) fault near the eastern end of the HFS. During the 3 months following the M6.9 earthquake, up to ~30 cm of slip occurred along the KFS, which helps accommodate rapid large‐scale subsidence of Kīlauea's summit region as large volumes of summit reservoir magma fed the lower East Rift Zone eruption. The HFS appears to activate only in concert with large earthquakes on the basal detachment. The KFS, on the other hand, moves both seismically during small local earthquakes and aseismically in response to nearby earthquakes and caldera subsidence

Water and sanitation: an essential battlefront in the war on antimicrobial resistance

Water and sanitation represent a key battlefront in combatting the spread of antimicrobial resistance (AMR). Basic water sanitation infrastructure is an essential first step towards protecting public health, thereby limiting the spread of pathogens and the need for antibiotics. AMR presents unique human health risks, meriting new risk assessment frameworks specifically adapted to water and sanitation-borne AMR. There are numerous exposure routes to AMR originating from human waste, each of which must be quantified for its relative risk to human health. Wastewater treatment plants play a vital role in centralized collection and treatment of human sewage, but there are numerous unresolved issues in terms of the microbial ecological processes occurring within them and the extent to which they attenuate or amplify AMR. Research is needed to advance understanding of the fate of resistant bacteria and antibiotic resistance genes in various waste management systems, depending on the local constraints and intended reuse applications. World Health Organization and national AMR action plans would benefit from a more holistic 'One Water' understanding. In this article we provide a framework for research, policy, practice and public engagement aimed at limiting the spread of AMR from water and sanitation in low-, medium- and high-income countries

Heterogeneous coupling of the Sumatran megathrust constrained by geodetic and paleogeodetic measurements

Geodetic and paleogeodetic measurements of interseismic strain above the Sumatran portion of the Sunda subduction zone reveal a heterogeneous pattern of coupling. Annual banding in corals provides vertical rates of deformation spanning the last half of the 20th century, and repeated GPS surveys between 1991 and 2001 and continuous measurements at GPS stations operated since 2002 provide horizontal velocities. Near the equator, the megathrust is locked over a narrow width of only a few tens of kilometers. In contrast, the locked fault zone is up to about 175 km wide in areas where great interplate earthquakes have occurred in the past. Formal inversion of the data reveals that these strongly coupled patches are roughly coincident with asperities that ruptured during these events. The correlation is most spectacular for rupture of the M_w 8.7 Nias-Simeulue earthquake of 2005, which released half of the moment deficit that had accumulated since its previous rupture in 1861, suggesting that this earthquake was overdue. Beneath the Mentawai islands, strong coupling is observed within the overlapping rupture areas of the great earthquakes of 1797 and 1833. The accumulated slip deficit since these events is slowly reaching the amount of slip that occurred during the 1833 earthquake but already exceeds the slip that occurred during the 1797 earthquake. Thus, rerupture of part of the Mentawai patch in September 2007 was not a surprise. In contrast, coupling is low below the Batu islands near the equator and around Enggano island at about 5°S, where only moderate earthquakes (M_w < 8.0) have occurred in the past two centuries. The correlation of large seismic asperities with patches that are locked during the interseismic period suggests that they are persistent features. This interpretation is reinforced by the fact that the large locked patches and great ruptures occur beneath persistent geomorphologic features, the largest outer arc islands. Depth- and convergence-rate-dependent temperature might influence the pattern of coupling, through its effect on the rheology of the plate interface, but other influences are required to account for the observed along-strike heterogeneity of coupling. In particular, subduction of the Investigator Fracture Zone could be the cause for the low coupling near the equator

Impacts of organic and conventional crop management on diversity and activity of free-living nitrogen fixing bacteria and total bacteria are subsidiary to temporal effects

A three year field study (2007-2009) of the diversity and numbers of the total and metabolically active free-living diazotophic bacteria and total bacterial communities in organic and conventionally managed agricultural soil was conducted at the Nafferton Factorial Systems Comparison (NFSC) study, in northeast England. The result demonstrated that there was no consistent effect of either organic or conventional soil management across the three years on the diversity or quantity of either diazotrophic or total bacterial communities. However, ordination analyses carried out on data from each individual year showed that factors associated with the different fertility management measures including availability of nitrogen species, organic carbon and pH, did exert significant effects on the structure of both diazotrophic and total bacterial communities. It appeared that the dominant drivers of qualitative and quantitative changes in both communities were annual and seasonal effects. Moreover, regression analyses showed activity of both communities was significantly affected by soil temperature and climatic conditions. The diazotrophic community showed no significant change in diversity across the three years, however, the total bacterial community significantly increased in diversity year on year. Diversity was always greatest during March for both diazotrophic and total bacterial communities. Quantitative analyses using qPCR of each community indicated that metabolically active diazotrophs were highest in year 1 but the population significantly declined in year 2 before recovering somewhat in the final year. The total bacterial population in contrast increased significantly each year. Seasonal effects were less consistent in this quantitative study

Lower edge of locked Main Himalayan Thrust unzipped by the 2015 Gorkha earthquake

Large earthquakes are thought to release strain on previously locked faults. However, the details of how earthquakes are initiated, grow and terminate in relation to pre-seismically locked and creeping patches is unclear ^1-4. The 2015 Mw 7.8 Gorkha, Nepal earthquake occurred close to Kathmandu in a region where the prior pattern of fault locking is well documented ^5. Here we analyze this event using seismological records measured at teleseismic distances and Synthetic Aperture Radar imagery. We show that the earthquake originated northwest of Kathmandu within a cluster of background seismicity that fringes the bottom of the locked portion of the Main Himalayan Thrust fault (MHT). The rupture propagated eastwards for about 140 km, unzipping the lower edge of the locked portion of the fault. High-frequency seismic waves radiated continuously as the slip pulse propagated at about 2.8 km s-1 along this zone of presumably high and heterogeneous pre-¬seismic stress at the seismic-aseismic transition. Eastward unzipping of the fault resumed during the Mw 7.3 aftershock on May 12. The transfer of stress to neighbouring regions during the Gorkha earthquake should facilitate future rupture of the areas of the MHT adjacent and up-dip of the Gorkha earthquake rupture.This is the author accepted manuscript. The final version is available from Nature Publishing Group via http://dx.doi.org/10.1038/ngeo251