The reproductive potential of vitrifiedwarmed euploid embryos declines following repeated uterine transfers

Background Recurrent implantation failure (RIF) represents a vague clinical condition with an unclear diagnostic challenge that lacks solid scientific underpinning. Although euploid embryos have demonstrated consistent implantation capabilities across various age groups, a unanimous agreement regarding the advantages of preimplantation genetic testing for aneuploidy (PGT-A) in managing RIF is absent. The ongoing discussion about whether chromosomal aneuploidy in embryos significantly contributes to recurrent implantation failure remains unsettled. Despite active discussions in recent times, a universally accepted characterization of recurrent implantation failure remains elusive. We aimed in this study to measure the reproductive performance of vitrified-warmed euploid embryos transferred to the uterus in successive cycles. Methods This observational cohort study included women (n = 387) with an anatomically normal uterus who underwent oocyte retrieval for PGT-A treatment with at least one biopsied blastocyst, between January 2017 and December 2021 at a university-affiliated public fertility center. The procedures involved in this study included ICSI, blastocyst culture, trophectoderm biopsy and comprehensive 24-chromosome analysis of preimplantation embryos using Next Generation Sequencing (NGS). Women, who failed a vitrified-warmed euploid embryo transfer, had successive blastocyst transfer cycles (FET) for a total of three using remaining cryopreserved euploid blastocysts from the same oocyte retrieval cycle. The primary endpoints were sustained implantation rate (SIR) and live birth rate (LBR) per vitrified-warmed single euploid embryo. The secondary endpoints were mean euploidy rate (m-ER) per cohort of biopsied blastocysts from each patient, as well as pregnancy and miscarriage rates. Results The mean age of the patient population was 33.4 years (95% CI 32.8–33.9). A total of 1,641 embryos derived from the first oocyte retrieval cycle were biopsied and screened. We found no associations between the m-ER and the number of previous failed IVF cycles among different ranges of maternal age at oocyte retrieval (P = 0.45). Pairwise comparisons showed a significant decrease in the sustained implantation rate (44.7% vs. 30%; P = 0.01) and the livebirth rate per single euploid blastocyst (37.1% vs. 25%; P = 0.02) between the 1st and 3rd FET. The cumulative SIR and LBR after up to three successive single embryo transfers were 77.1% and 68.8%, respectively. We found that the live birth rate of the first vitrified-warmed euploid blastocyst transferred decreased significantly with the increasing number of previously failed IVF attempts by categories (45.3% vs. 35.8% vs. 27.6%; P = 0.04). A comparable decrease in sustained implantation rate was also observed but did not reach statistical significance (50% vs. 44.2 vs. 37.9%; P = NS). Using a logistic regression model, we confirmed the presence of a negative association between the number of previous IVF failed attempts and the live birth rate per embryo transfer cycle (OR = 0.76; 95% CI 0.62–0.94; P = 0.01). Conclusions These findings are vital for enhancing patient counseling and refining management strategies for individuals facing recurrent implantation failure. By tailoring interventions based on age and ovarian reserve, healthcare professionals can offer more personalized guidance, potentially improving the overall success rates and patient experiences in fertility treatments

An implementation of an aeroacoustic prediction model for broadband noise from a vertical axis wind turbine using a CFD informed methodology

This paper presents an enhanced method for predicting aerodynamically generated broadband noise produced by a Vertical Axis Wind Turbine (VAWT). The method improves on existing work for VAWT noise prediction and incorporates recently developed airfoil noise prediction models. Inflow-turbulence and airfoil self-noise mechanisms are both considered. Airfoil noise predictions are dependent on aerodynamic input data and time dependent Computational Fluid Dynamics (CFD) calculations are carried out to solve for the aerodynamic solution. Analytical ow methods are also benchmarked against the CFD informed noise prediction results to quantify errors in the former approach. Comparisons to experimental noise measurements for an existing turbine are encouraging. A parameter study is performed and shows the sensitivity of overall noise levels to changes in inflow velocity and inflow turbulence. Noise sources are characterised and the location and mechanism of the primary sources is determined, inflow-turbulence noise is seen to be the dominant source. The use of CFD calculations is seen to improve the accuracy of noise predictions when compared to the analytic ow solution as well as showing that, for inflow-turbulence noise sources, blade generated turbulence dominates the atmospheric inflow turbulence

COVID-19 first lockdown as a window into language acquisition: Associations between caregiver-child activities and vocabulary gains

The COVID-19 pandemic, and the resulting closure of daycare centers worldwide, led to unprecedented changes in children’s learning environments. This period of increased time at home with caregivers, with limited access to external sources (e.g., daycares) provides a unique opportunity to examine the associations between the caregiver-child activities and children’s language development. The vocabularies of 1742 children aged8-36 months across 13 countries and 12 languages were evaluated at the beginning and end of the first lockdown period in their respective countries(from March to September 2020). Children who had less passive screen exposure and whose caregivers read more to them showed larger gains in vocabulary development during lockdown, after controlling for SES and other caregiver-child activities. Children also gained more words than expected (based on normative data) during lockdown; either caregivers were more aware of their child’s development or vocabulary development benefited from intense caregiver-child interaction during lockdown

Spreading of low-viscous liquids on a stationary and a moving surface

Abstract: This paper examines the time evolution for spreading of low surface tension liquids upon impact onto a surface, and highlights the differences with the same, for high surface tension liquids. Furthermore, it examines the role of the in-plane velocity (V P ) on the time evolution of spreading phase of the impact phenomena; V P is seen when the surface is inclined, or when the surface is moving in the horizontal direction, for impact of a free-falling droplet. High-speed imaging was used to capture the spreading phenomenon from side and overhead views. It was observed that low and high surface tension liquids spread in a different manner on both stationary and moving surfaces with different outcomes regarding the time to the maximum spreading diameter, and the maximum spreading factor. Also, compared to high surface tension liquids, the stretching of lamella in the direction of the in-plane velocity vector, is more pronounced for low surface tension liquids. We observed that on a moving surface, the position of the maximum width shifts more to the center of the lamella for low surface tension liquids, compared to that of high surface tension liquids, and this shifting increases with an increase in in-plane velocity. We developed a method and related equations to describe the time evolution of the lamella as drop spreads on a hydrophilic surface. Using our method, one can predict the spreading of both low and high surface tension liquids over stationary and moving surfaces (i.e., when in-plane velocity exists). Graphical abstract: [Figure not available: see fulltext.] Top view of droplets impacting a moving surface: Same Impact Conditions, but different shapes for spreading, and time to max spreading seen, and explained

A zSlices-based general type-2 fuzzy logic system for users-centric adaptive learning in large-scale e-learning platforms

Sophisticated educational technologies are evolving rapidly, and online courses are becoming more easily available, generating interest in innovating lightweight data-driven adaptive approaches that foster responsive teaching and improving the overall learning experience. However, in most existing adaptive educational systems, the black-box modeling of learner and instructional models based on the views of a few designers or experts tended to drive the adaptation of learning content. However, different sources of uncertainty could affect these views, including how accurately the proposed adaptive educational methods actually assess student responses and the corresponding uncertainties associated with how students receive and comprehend the resulting instruction. E-learning environments contain high levels of linguistic uncertainties, whereby students can interpret and act on the same terms, words, or methods (e.g., course difficulty, length of study time, or preferred learning style) in various ways according to varying levels of motivation, pre-knowledge, cognition, and future plans. Thus, one adaptive instructional model does not fit the needs of all students. Basing the instruction model on determining learners’ interactions within the learning environment in interpretable and easily read white-box models is crucial for adapting the model to students’ needs and understanding how learning is realized. This paper presents a new zSlices-based type-2 fuzzy-logic-based system that can learn students’ preferred knowledge delivery needs based on their characteristics and current levels of knowledge to generate an adaptive learning environment. We have evaluated the proposed system’s efficiency through various large-scale, real-world experiments involving 1871 students from King Abdulaziz University. These experiments demonstrate the proposed zSlices type-2 fuzzy-logic-based system’s capability for handling linguistic uncertainties to produce better performance, particularly in terms of enhanced student performance and improved success rates compared with interval type-2 fuzzy logic, type-1 fuzzy systems, adaptive, instructor-led systems, and non-adaptive systems

The reproductive potential of vitrified-warmed euploid embryos declines following repeated uterine transfers

Abstract Background Recurrent implantation failure (RIF) represents a vague clinical condition with an unclear diagnostic challenge that lacks solid scientific underpinning. Although euploid embryos have demonstrated consistent implantation capabilities across various age groups, a unanimous agreement regarding the advantages of preimplantation genetic testing for aneuploidy (PGT-A) in managing RIF is absent. The ongoing discussion about whether chromosomal aneuploidy in embryos significantly contributes to recurrent implantation failure remains unsettled. Despite active discussions in recent times, a universally accepted characterization of recurrent implantation failure remains elusive. We aimed in this study to measure the reproductive performance of vitrified-warmed euploid embryos transferred to the uterus in successive cycles. Methods This observational cohort study included women (n = 387) with an anatomically normal uterus who underwent oocyte retrieval for PGT-A treatment with at least one biopsied blastocyst, between January 2017 and December 2021 at a university-affiliated public fertility center. The procedures involved in this study included ICSI, blastocyst culture, trophectoderm biopsy and comprehensive 24-chromosome analysis of preimplantation embryos using Next Generation Sequencing (NGS). Women, who failed a vitrified-warmed euploid embryo transfer, had successive blastocyst transfer cycles (FET) for a total of three using remaining cryopreserved euploid blastocysts from the same oocyte retrieval cycle. The primary endpoints were sustained implantation rate (SIR) and live birth rate (LBR) per vitrified-warmed single euploid embryo. The secondary endpoints were mean euploidy rate (m-ER) per cohort of biopsied blastocysts from each patient, as well as pregnancy and miscarriage rates. Results The mean age of the patient population was 33.4 years (95% CI 32.8–33.9). A total of 1,641 embryos derived from the first oocyte retrieval cycle were biopsied and screened. We found no associations between the m-ER and the number of previous failed IVF cycles among different ranges of maternal age at oocyte retrieval (P = 0.45). Pairwise comparisons showed a significant decrease in the sustained implantation rate (44.7% vs. 30%; P = 0.01) and the livebirth rate per single euploid blastocyst (37.1% vs. 25%; P = 0.02) between the 1st and 3rd FET. The cumulative SIR and LBR after up to three successive single embryo transfers were 77.1% and 68.8%, respectively. We found that the live birth rate of the first vitrified-warmed euploid blastocyst transferred decreased significantly with the increasing number of previously failed IVF attempts by categories (45.3% vs. 35.8% vs. 27.6%; P = 0.04). A comparable decrease in sustained implantation rate was also observed but did not reach statistical significance (50% vs. 44.2 vs. 37.9%; P = NS). Using a logistic regression model, we confirmed the presence of a negative association between the number of previous IVF failed attempts and the live birth rate per embryo transfer cycle (OR = 0.76; 95% CI 0.62–0.94; P = 0.01). Conclusions These findings are vital for enhancing patient counseling and refining management strategies for individuals facing recurrent implantation failure. By tailoring interventions based on age and ovarian reserve, healthcare professionals can offer more personalized guidance, potentially improving the overall success rates and patient experiences in fertility treatments. Trial registration number N/A

A type-2 fuzzy logic recommendation system for adaptive teaching

E-learning platforms facilitate the interaction between students and instructors while mitigating temporal or spatial constraints. Nevertheless, such platforms require measuring the degree of students’ engagement with the delivered course content and teaching style. Such information is highly valuable for evaluating the quality of the teaching and altering the teaching delivery style in massively crowded online learning platforms. When the number of learners is high, it is essential to attain overall engagement and feedback, yet doing so is highly challenging due to the high levels of uncertainties related to students and the learning context. To handle these uncertainties more robustly, we present a method based on type-2 fuzzy logic utilizing visual RGB-D features, including head pose direction and facial expressions captured from Kinect v2, a low-cost but robust 3D camera, to measure the engagement degree of students in both remote and on-site education. This system augments another self-learning type-2 fuzzy logic system that helps teachers with recommendations of how to adaptively vary their teaching methods to suit the level of students and enhance their instruction delivery. This proposed dynamic e-learning environment integrates both on-site and distance students as well as teachers who instruct both groups of students. The rules are learned from the students’ and teachers’ learning/teaching behaviors, and the system is continuously updated to give the teacher the ability to adapt the delivery approach to varied learners’ engagement levels. The efficiency of the proposed system has been tested through various real-world experiments in the University of Essex iClassroom among a group of thirty students and six teachers. These experiments demonstrate the capabilities—compared to type-1 fuzzy systems and non-adaptive systems—of the proposed interval type-2 fuzzy logic-based system to handle the uncertainties and improve average learners’ motivations to engage during learning

Clinical features and outcome of human Mpox (Monkeypox) in Saudi Arabia: An observational study of travel-related cases

Background: The 2022 Monkeypox virus (Mpox) outbreak had involved multiple countries around the globe. Here, we report clinical features and outcome of human Mpox of the first cases in Saudi Arabia. Methods: We obtained records of confirmed Mpox cases in Saudi Arabia from the public electronic health information system, Health Electronic Surveillance Network (HESN) and the healthcare providers completed a de-identified structured clinical data collection form. Results: The reported seven cases were travel-related and all were males between 24 and 41 years of age (mean age + SD) was 30.14 (+ 6.69) years. Of the cases, three (43 %) had heterosexual contact and the others had other intimate encounters while traveling abroad. They presented with skin lesions (100 %), fever (86 %), and lymphadenopathy (71 %). The illness was mild to moderate, did not require antiviral medications, and lasted 7–15 days. The mean duration of skin rash (+ SD) was 10 (+ 2.68) days. Routine laboratory tests (CBC, BUN, serum electrolytes, and liver enzymes) were within normal limits, and initial screening for HIV was negative. Expanded contact tracing did not reveal secondary cases of Mpox in the community or the healthcare setting. Conclusion: The current study showed heterosexual transmission of Mpox and the clinical course was mild and non-complicated. Therefore, clinicians and public health professionals should consider Mpox among individuals presenting with skin rash especially in the context of the investigation of HIV and other sexually transmitted diseases

Image Retrieval by Use of Linguistic Description in Databases

In this paper, a new method of image retrieval is proposed. This concerns retrieving color digital images from a database that contains a specific linguistic description considered within the theory of fuzzy granulation and computing with words. The linguistic description is generated by use of the CIE chromaticity color model. The image retrieval is performed in different way depending on users' knowledge about the color image. Specific database queries can be formulated for the image retrieval