Additive manufacturing of an oxide ceramic by laser beam melting—Comparison between finite element simulation and experimental results

International audienceRecent progress in the application of Laser Beam Melting (LBM) of oxide ceramics has shown promising results. However, a deeper understanding of the process is required to master and control the track development. In this approach numerical modeling could allow higher quality, of additive manufacturing for such materials, to be achieved. The validation of an earlier developed finite element model for LBM of ceramic materials has been established through a comparison with experimental results. The model solves heat and mass transfers whilst accounting for fluid flow due to surface tension and Marangoni convection, as well as tracking the material/gas boundary. The volumetric heat source parameters used in the simulations have been calibrated with an analytical model combined with original in-situ reflectance measurements. Numerical results show good agreement with measurements of melt pool dimensions and shapes. They also provide a coherent description of the evolution of the track morphology when varying the heat source parameters. Track irregularities have also been revealed by simulations at high scanning speed and the balling effect highlighted and explained through similar simulations

Dissecting the transcriptional phenotype of ribosomal protein deficiency: implications for Diamond-Blackfan Anemia

Defects in genes encoding ribosomal proteins cause Diamond Blackfan Anemia (DBA), a red cell aplasia often associated with physical abnormalities. Other bone marrow failure syndromes have been attributed to defects in ribosomal components but the link between erythropoiesis and the ribosome remains to be fully defined. Several lines of evidence suggest that defects in ribosome synthesis lead to "ribosomal stress" with p53 activation and either cell cycle arrest or induction of apoptosis. Pathways independent of p53 have also been proposed to play a role in DBA pathogenesis. We took an unbiased approach to identify p53-independent pathways activated by defects in ribosome synthesis by analyzing global gene expression in various cellular models of DBA. Ranking-Principal Component Analysis (Ranking-PCA) was applied to the identified datasets to determine whether there are common sets of genes whose expression is altered in these different cellular models. We observed consistent changes in the expression of genes involved in cellular amino acid metabolic process, negative regulation of cell proliferation and cell redox homeostasis. These data indicate that cells respond to defects in ribosome synthesis by changing the level of expression of a limited subset of genes involved in critical cellular processes. Moreover, our data support a role for p53-independent pathways in the pathophysiology of DBA

Etude de la prolifération et de la différenciation érythroïde in vitro et de la potentielle implication de la voie de p53 au cours de l'anémie de Blackfan-Diamond (ABD)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Laser beam melting of alumina : effect of absorber additions

International audienceCeramic laser beam melting offers new manufacturing possibilities for complex refractory structures. Poor absorptivity in near infra-red wavelengths of oxide ceramics is overcome with absorber addition to ceramic powders. Absorbers affect powder bed densities and geometrical stability of melted tracks. Optimum absorber content is defined for Al2O3 by minimizing powder bed porosity, maximizing melting pool geometrical stability and limiting shrinkage. Widest stability fields are obtained with addition of 0.1 wt.% C and 0.5 wt.% β-SiC. Absorption coefficient values of Beer-Lambert law follow stability trends: they increase with C additions, whereas with β-SiC, a maximum is reached for 0.5 wt.%. Powder particle ejections are also identified. Compared to metallic materials, this ejection phenomenon can no longer be neglected when establishing a three-dimensional manufacturing strategy

Impact of food grade and nano-TiO2 on human gut microbiota

International audienc

Impact of food grade and nano-TiO2 on human gut microbiota

International audienc