A Gas Giant Circumbinary Planet Transiting the F Star Primary of the Eclipsing Binary Star KIC 4862625 and the Independent Discovery and Characterization of the two transiting planets in the Kepler-47 System

We report the discovery of a transiting, gas giant circumbinary planet orbiting the eclipsing binary KIC 4862625 and describe our independent discovery of the two transiting planets orbiting Kepler-47 (Orosz et al. 2012). We describe a simple and semi-automated procedure for identifying individual transits in light curves and present our follow-up measurements of the two circumbinary systems. For the KIC 4862625 system, the 0.52+/-0.018 RJup radius planet revolves every ~138 days and occults the 1.47+/-0.08 MSun, 1.7 +/-0.06 RSun F8 IV primary star producing aperiodic transits of variable durations commensurate with the configuration of the eclipsing binary star. Our best-fit model indicates the orbit has a semi-major axis of 0.64 AU and is slightly eccentric, e=0.1. For the Kepler-47 system, we confirm the results of Orosz et al. (2012). Modulations in the radial velocity of KIC 4862625A are measured both spectroscopically and photometrically, i.e. via Doppler boosting, and produce similar results.Comment: 40 pages, 17 figure

RNA-seq Brings New Insights to the Intra-Macrophage Transcriptome of Salmonella Typhimurium.

Salmonella enterica serovar Typhimurium is arguably the world's best-understood bacterial pathogen. However, crucial details about the genetic programs used by the bacterium to survive and replicate in macrophages have remained obscure because of the challenge of studying gene expression of intracellular pathogens during infection. Here, we report the use of deep sequencing (RNA-seq) to reveal the transcriptional architecture and gene activity of Salmonella during infection of murine macrophages, providing new insights into the strategies used by the pathogen to survive in a bactericidal immune cell. We characterized 3583 transcriptional start sites that are active within macrophages, and highlight 11 of these as candidates for the delivery of heterologous antigens from Salmonella vaccine strains. A majority (88%) of the 280 S. Typhimurium sRNAs were expressed inside macrophages, and SPI13 and SPI2 were the most highly expressed pathogenicity islands. We identified 31 S. Typhimurium genes that were strongly up-regulated inside macrophages but expressed at very low levels during in vitro growth. The SalComMac online resource allows the visualisation of every transcript expressed during bacterial replication within mammalian cells. This primary transcriptome of intra-macrophage S.-Typhimurium describes the transcriptional start sites and the transcripts responsible for virulence traits, and catalogues the sRNAs that may play a role in the regulation of gene expression during infection

SOPHIE velocimetry of Kepler transit candidates XVI. Tomographic measurement of the low obliquity of KOI-12b, a warm Jupiter transiting a fast rotator

We present the detection and characterization of the transiting warm Jupiter KOI-12b, first identified with Kepler with an orbital period of 17.86 days. We combine the analysis of Kepler photometry with Doppler spectroscopy and line-profile tomography of time-series spectra obtained with the SOPHIE spectrograph to establish its planetary nature and derive its properties. To derive reliable estimates for the uncertainties on the tomographic model parameters, we devised an empirical method to calculate statistically independent error bars on the time-series spectra. KOI-12b has a radius of 1.43$\pm$0.13$R_\mathrm{Jup}$ and a 3$\sigma$ upper mass limit of 10$M_\mathrm{Jup}$. It orbits a fast-rotating star ($v$sin$i_{\star}$ = 60.0$\pm$0.9 km s$^{-1}$) with mass and radius of 1.45$\pm$0.09 $M_\mathrm{Sun}$ and 1.63$\pm$0.15 $R_\mathrm{Sun}$, located at 426$\pm$40 pc from the Earth. Doppler tomography allowed a higher precision on the obliquity to be reached by comparison with the analysis of the Rossiter-McLaughlin radial velocity anomaly, and we found that KOI-12b lies on a prograde, slightly misaligned orbit with a low sky-projected obliquity $\lambda$ = 12.6$\stackrel{+3.0}{_{-2.9}}^\circ$. The properties of this planetary system, with a 11.4 magnitude host-star, make of KOI-12b a precious target for future atmospheric characterization.Comment: 19 pages, 10 figure

One of the closest exoplanet pairs to the 3:2 Mean Motion Resonance: K2-19b \& c

The K2 mission has recently begun to discover new and diverse planetary systems. In December 2014 Campaign 1 data from the mission was released, providing high-precision photometry for ~22000 objects over an 80 day timespan. We searched these data with the aim of detecting further important new objects. Our search through two separate pipelines led to the independent discovery of K2-19b \& c, a two-planet system of Neptune sized objects (4.2 and 7.2 $R_\oplus$), orbiting a K dwarf extremely close to the 3:2 mean motion resonance. The two planets each show transits, sometimes simultaneously due to their proximity to resonance and alignment of conjunctions. We obtain further ground based photometry of the larger planet with the NITES telescope, demonstrating the presence of large transit timing variations (TTVs), and use the observed TTVs to place mass constraints on the transiting objects under the hypothesis that the objects are near but not in resonance. We then statistically validate the planets through the \texttt{PASTIS} tool, independently of the TTV analysis.Comment: 18 pages, 10 figures, accepted to A&A, updated to match published versio

The SOPHIE search for northern extrasolar planets XIV. A temperate (Teq ~ 300 K) super-earth around the nearby star Gliese 411

Periodic radial velocity variations in the nearby M-dwarf star Gl 411 are reported, based on measurements with the SOPHIE spectrograph. Current data do not allow us to distinguish between a 12.95-day period and its one-day alias at 1.08 days, but favour the former slightly. The velocity variation has an amplitude of 1.6 m s−1, making this the lowest-amplitude signal detected with SOPHIE up to now. We have performed a detailed analysis of the significance of the signal and its origin, including extensive simulations with both uncorrelated and correlated noise, representing the signal induced by stellar activity. The signal is significantly detected, and the results from all tests point to its planetary origin. Additionally, the presence of an additional acceleration in the velocity time series is suggested by the current data. On the other hand, a previously reported signal with a period of 9.9 days, detected in HIRES velocities of this star, is not recovered in the SOPHIE data. An independent analysis of the HIRES dataset also fails to unveil the 9.9-day signal. If the 12.95-day period is the real one, the amplitude of the signal detected with SOPHIE implies the presence of a planet, called Gl 411 b, with a minimum mass of around three Earth masses, orbiting its star at a distance of 0.079 AU. The planet receives about 3.5 times the insolation received by Earth, which implies an equilibrium temperature between 256 and 350 K, and makes it too hot to be in the habitable zone. At a distance of only 2.5 pc, Gl 411 b, is the third closest low-mass planet detected to date. Its proximity to Earth will permit probing its atmosphere with a combination of high-contrast imaging and high-dispersion spectroscopy in the next decade

KPS-1b: The First Transiting Exoplanet Discovered Using an Amateur Astronomer's Wide-field CCD Data

We report the discovery of the transiting hot Jupiter KPS-1b. This exoplanet orbits a V = 13.0 K1-type main sequence star every 1.7 days, has a mass of 1.090 (+0.086 -0.087) MJup and a radius of 1.03 (+0.13 -0.12) RJup. The discovery was made by the prototype Kourovka Planet Search (KPS) project, which used wide-field CCD data gathered by an amateur astronomer using readily available and relatively affordable equipment. Here we describe the equipment and observing technique used for the discovery of KPS-1b, its characterization with spectroscopic observations by the SOPHIE spectrograph and with high-precision photometry obtained with 1m class telescopes. We also outline the KPS project evolution into the Galactic Plane eXoplanet survey. The discovery of KPS-1b represents a new major step of the contribution of amateur astronomers to the burgeoning field of exoplanetology

Two warm Neptunes transiting HIP 9618 revealed by TESS and Cheops

peer reviewedHIP 9618 (HD 12572, TOI-1471, TIC 306263608) is a bright (G = 9.0 mag) solar analogue. TESS photometry revealed the star to have two candidate planets with radii of 3.9 ± 0.044 R (HIP 9618 b) and 3.343 ± 0.039 R (HIP 9618 c). While the 20.77291 d period of HIP 9618 b was measured unambiguously, HIP 9618 c showed only two transits separated by a 680-d gap in the time series, leaving many possibilities for the period. To solve this issue, CHEOPS performed targeted photometry of period aliases to attempt to recover the true period of planet c, and successfully determined the true period to be 52.56349 d. High-resolution spectroscopy with HARPS-N, SOPHIE, and CAFE revealed a mass of 10.0 ± 3.1M for HIP 9618 b, which, according to our interior structure models, corresponds to a 6.8 ± 1.4 per cent gas fraction. HIP 9618 c appears to have a lower mass than HIP 9618 b, with a 3-sigma upper limit of 50 d, opening the door for the atmospheric characterization of warm (Teq < 750 K) sub-Neptunes

Redundant Hydrogen Peroxide Scavengers Contribute to Salmonella Virulence and Oxidative Stress Resistance▿ †

Salmonella enterica serovar Typhimurium is an intracellular pathogen that can survive and replicate within macrophages. One of the host defense mechanisms that Salmonella encounters during infection is the production of reactive oxygen species by the phagocyte NADPH oxidase. Among them, hydrogen peroxide (H2O2) can diffuse across bacterial membranes and damage biomolecules. Genome analysis allowed us to identify five genes encoding H2O2 degrading enzymes: three catalases (KatE, KatG, and KatN) and two alkyl hydroperoxide reductases (AhpC and TsaA). Inactivation of the five cognate structural genes yielded the HpxF− mutant, which exhibited a high sensitivity to exogenous H2O2 and a severe survival defect within macrophages. When the phagocyte NADPH oxidase was inhibited, its proliferation index increased 3.7-fold. Moreover, the overexpression of katG or tsaA in the HpxF− background was sufficient to confer a proliferation index similar to that of the wild type in macrophages and a resistance to millimolar H2O2 in rich medium. The HpxF− mutant also showed an attenuated virulence in a mouse model. These data indicate that Salmonella catalases and alkyl hydroperoxide reductases are required to degrade H2O2 and contribute to the virulence. This enzymatic redundancy highlights the evolutionary strategies developed by bacterial pathogens to survive within hostile environments

Abnormal metabolism flexibility in response to high palmitate concentrations in myotubes derived from obese type 2 diabetic patients

International audienceInsulin resistance in type 2 diabetes (T2D) is associated with intramuscular lipid (IMCL) accumulation. To determine whether impaired lipid oxidation is involved in IMCL accumulation, we measured expression of genes involved in mitochondrial oxidative metabolism or biogenesis, mitochondrial content and palmitate beta-oxidation before and after palmitate overload (600 μM for 16h), in myotubes derived from healthy subjects and obese T2D patients. Mitochondrial gene expression, content and network were not different between groups. Basal palmitate beta-oxidation was not affected in T2D myotubes, whereas after 16h of palmitate pre-treatment, T2D myotubes in contrast to control myotubes, showed an inability to increase palmitate beta-oxidation (p<0.05). Interestingly, acetyl-CoA carboxylase (ACC) phosphorylation was increased with a tendency for statistical significance after palmitate pre-treatment in control myotubes (p=0.06) but not in T2D myotubes which can explain their inability to increase palmitate beta-oxidation after palmitate overload. To determine whether the activation of the AMP activated protein kinase (AMPK)-ACC pathway was able to decrease lipid content in T2D myotubes, cells were treated with AICAR and metformin. These AMPK activators had no effect on ACC and AMPK phosphorylation in T2D myotubes as well as on lipid content, whereas AICAR, but not metformin, increased AMPK phosphorylation in control myotubes. Interestingly, metformin treatment and mitochondrial inhibition by antimycin induced increased lipid content in control myotubes. We conclude that T2D myotubes display an impaired capacity to respond to metabolic stimuli

Salmonella detoxifying enzymes are sufficient to cope with the host oxidative burst.

International audienceThe oxidative burst produced by the NADPH oxidase (Phox) is an essential weapon used by host cells to eradicate engulfed pathogens. In Salmonella typhimurium, oxidative stress resistance has been previously proposed to be mediated by the pathogenicity island 2 type III secretion system (T3SS-2), periplasmic superoxide dismutases and cytoplasmic catalases/peroxidases. Here, we fused an OxyR-dependent promoter to the gfp to build the ahpC-gfp transcriptional fusion. This reporter was used to monitor hydrogen peroxide levels as sensed by Salmonella during the course of an infection. We showed that the expression of this fusion was under the exclusive control of reactive oxygen species produced by the host. The ahpC-gfp expression was noticeably modified in the absence of bacterial periplasmic superoxide dismutases or cytoplasmic catalases/peroxidases. Surprisingly, inactivation of the T3SS-2 had no effect on the ahpC-gfp expression. All together, these results led to a model in which Salmonella resistance relies on its arsenal of detoxifying enzymes to cope with Phox-mediated oxidative stress