Estudio sedimentológico y paleontológico de la zona de los «Freus de Ibiza»

El estudio sedimentológico de los depósitos sublitorales de la zona de los «Freus de Ibiza», entre las islas de Ibiza y Formentera, muestra que se trata de un área constituida por materiales arenosos de composición calcárea (bioclastos), muy ricos en fragmentos de conchas de moluscos y foraminíferos bentónicos, asociados a «praderas de posidonias». Se han podido diferenciar tres «facies franulométricas», según el tamaño medio de grano y grado de selección del sedimento, correspondiendo los materiales más finos y mejor seleccionados a las zonas más cercanas a la costa, y tres «facies paleontológicas, según el contenido en foraminíferos, correspondiendo los máximos contenidos a las áreas de menor profundidad más cerca de la costa, por la acumulación en ellas de los restos procedentes de la zona de «praderas de posidonias», en aguas más profundas, donde normalmente habitan estos organismos.The study of recent sediments beneath the coastline between the island of Ibiza and Formentera («Freus de Ibiza») shows they consist of a chalk-sandy material (bioclastics) and are very rich in phoraminifers associated with «posidonian meadows» and fragments of mollusc's shells. Three types or «facies» of sediments have been classified according to their granulometry, and three paleontologycal facies according to their phoraminifer content, putting the finest chosen ones with a higher percentage of phoraminifers with those found in zones of greather profundity, nearer the coast due to the accumulation of what is left coming out from the deeper part of the zone of «posidonian meadows»

Early diagenesis in biogenic carbonates of temperate and shoal waters (Cadiz Bay and adjacent continental shelf)

Because of the chemical reactivity of biogenic carbonates in shallow waters, there is increasing interest in those early diagenesis processes that imply an alteration or variation of the composition of original material. This paper presents the preliminary results of a study concerning the compositional variations that have taken place in the skeletons or skeletal remains of bryozoans and echinoids. This variation, which includes an aragonite-calcite transformation, has been checked by means of the Rietveld method, using the Fullprof program. This transformation is caused by an in situ dissolution-recrystallisation process, due to aragonite's metastability. Moreover, the presence of specific authigenic minerals helps to delimit early diagenetic processes, which are, in turn, indicative of the path and the extension of the diagenetic reactions that take place in the sediment column.Debido a que los carbonatos biogénicos de aguas someras son químicamente muy reactivos, existe cada vez mayor interés por conocer los procesos de diagénesis temprana que conllevan una alteración o modificación de la composición del material original. En este trabajo se presentan los resultados iniciales sobre las variaciones de composición que han tenido lugar en los caparazones o restos del esqueleto de equinodermos y briozoos. Esa variación, incluida la transformación aragonito-calcita, ha sido chequeada mediante el método Rietveld, programa Fullprof. Dicha transformación ocurre por un proceso de disolución-recristalización debida a la metaestabilidad del aragonito. Además, la presencia de una asociación de minerales neoformados implica procesos diagenéticos tempranos indicativos de las reacciones que tienen lugar en la columna del sedimento.Instituto Español de Oceanografí

Continental shelf zones influenced by the suspended matter flows coming from Cadiz Bay

Due to the diversity of environments and hydrodynamics in Cadiz Bay, it is of interest to study the dynamics of sedimentary exchange between the coastal zones and continental shelf. The most abundant clay minerals on the floor of the continental shelf and Cadiz Bay, and in the particulate matter in suspension, are: illite, smectite, kaolinite + chlorite, and the interstratified clay minerals illite-smectite. The average concentration of suspended matter is 6.5 mg/l, the lowest (1.37 mg/l) being found on the inner shelf and in the inner zones of the bay less affected by currents and surge. The highest values (14 mg/l) are found in the outer bay, consistent with the distribution of muddy facies on the sea floor. The flow paths have been established from the local variations in the mineralogical associations found at the sampling stations, and by means of the alignments of the lowest values of the factor loadings at those stations most affected by the flows. The mineralogical similarity of the components of the clay fraction is related to the mixing action undergone by the components of the suspended matter reaching the bay from various sources, probably produced by the continuous action of the tidal ebb currents flowing from the inner sectors of the bay towards the continental shelf.El estudio de la dinámica de intercambio sedimentario entre las zonas litorales y la plataforma continental es de gran interés en el caso de la bahía de Cádiz, debido a su diversidad de ambientes y situaciones hidrodinámicas. Los minerales de la arcilla más abundantes en los fondos de la plataforma y la bahía, y en la materia en suspensión, son ilita, esmectita, caolinita + clorita e interestratificados ilita-esmectita. La concentración de materia sólida en suspensión indica un contenido promedio de 6,5 mg/l, apareciendo los mínimos (1,37 mg/l) en la plataforma interna y en zonas internas de la bahía no afectadas por corrientes y oleaje. Los valores más altos se dan en la bahía externa (14 mg/l), de acuerdo con la distribución de facies fangosas en el fondo. Las trayectorias de los flujos salientes se han establecido a partir de las variaciones locales de las asociaciones mineralógicas en las diferentes estaciones de muestreo, alineándose los valores más bajos de los factores en las estaciones más afectadas por la acción de los flujos. La similitud mineralógica de los componentes de la fracción arcilla en todos los ambientes se debe a la mezcla de componentes de la materia en suspensión que, desde diferentes fuentes, llegan a la bahía, y es favorecida por la acción de la corriente de reflujo mareal desde el sector más interno de la bahía a la plataforma continental.Instituto Español de Oceanografí

The alpha-galactosidase A p.Arg118Cys variant does not cause a Fabry disease phenotype: data from individual patients and family studies

Acessível em: www.ncbi.nlm.nih.gov/pmc/articles/PMC4423738/Lysosomal α-galactosidase A (α-Gal) is the enzyme deficient in Fabry disease (FD), an X-linked glycosphingolipidosis caused by pathogenic mutations affecting the GLA gene. The early-onset, multi-systemic FD classical phenotype is associated with absent or severe enzyme deficiency, as measured by in vitro assays, but patients with higher levels of residual α-Gal activity may have later-onset, more organ-restricted clinical presentations. A change in the codon 118 of the wild-type α-Gal sequence, replacing basic arginine by a potentially sulfhydryl-binding cysteine residue - GLA p.(Arg118Cys) -, has been recurrently described in large FD screening studies of high-risk patients. Although the Cys118 allele is associated with high residual α-Gal activity in vitro, it has been classified as a pathogenic mutation, mainly on the basis of theoretical arguments about the chemistry of the cysteine residue. However its pathogenicity has never been convincingly demonstrated by pathology criteria. We reviewed the clinical, biochemical and histopathology data obtained from 22 individuals of Portuguese and Spanish ancestry carrying the Cys118 allele, including 3 homozygous females. Cases were identified either on the differential diagnosis of possible FD manifestations and on case-finding studies (n=11; 4 males), or on unbiased cascade screening of probands' close relatives (n=11; 3 males). Overall, those data strongly suggest that the GLA p.(Arg118Cys) variant does not segregate with FD clinical phenotypes in a Mendelian fashion, but might be a modulator of the multifactorial risk of cerebrovascular disease. The Cys118 allelic frequency in healthy Portuguese adults (n=696) has been estimated as 0.001, therefore not qualifying for "rare" condition

Comparative Analysis of the Volatile Fraction of Fruit Juice from Different Citrus Species

The volatile composition of fruit from four Citrus varieties (Powell Navel orange, Clemenules mandarine, and Fortune mandarine and Chandler pummelo) covering four different species has been studied. Over one hundred compounds were profiled after HS-SPME-GC-MS analysis, including 27 esters, 23 aldehydes, 21 alcohols, 13 monoterpene hydrocarbons, 10 ketones, 5 sesquiterpene hydrocarbons, 4 monoterpene cyclic ethers, 4 furans, and 2 aromatic hydrocarbons, which were all confirmed with standards. The differences in the volatile profile among juices of these varieties were essentially quantitative and only a few compounds were found exclusively in a single variety, mainly in Chandler. The volatile profile however was able to differentiate all four varieties and revealed complex interactions between them including the participation in the same biosynthetic pathway. Some compounds (6 esters, 2 ketones, 1 furan and 2 aromatic hydrocarbons) had never been reported earlier in Citrus juices. This volatile profiling platform for Citrus juice by HS-SPME-GC-MS and the interrelationship detected among the volatiles can be used as a roadmap for future breeding or biotechnological applications

A longitudinal study of gene expression in first-episode schizophrenia; exploring relapse mechanisms by co-expression analysis in peripheral blood

Little is known about the pathophysiological mechanisms of relapse in first-episode schizophrenia, which limits the study of potential biomarkers. To explore relapse mechanisms and identify potential biomarkers for relapse prediction, we analyzed gene expression in peripheral blood in a cohort of first-episode schizophrenia patients with less than 5 years of evolution who had been evaluated over a 3-year follow-up period. A total of 91 participants of the 2EPs project formed the sample for baseline gene expression analysis. Of these, 67 provided biological samples at follow-up (36 after 3 years and 31 at relapse). Gene expression was assessed using the Clariom S Human Array. Weighted gene co-expression network analysis was applied to identify modules of co-expressed genes and to analyze their preservation after 3 years of follow-up or at relapse. Among the 25 modules identified, one module was semi-conserved at relapse (DarkTurquoise) and was enriched with risk genes for schizophrenia, showing a dysregulation of the TCF4 gene network in the module. Two modules were semi-conserved both at relapse and after 3 years of follow-up (DarkRed and DarkGrey) and were found to be biologically associated with protein modification and protein location processes. Higher expression of DarkRed genes was associated with higher risk of suffering a relapse and early appearance of relapse (p = 0.045). Our findings suggest that a dysregulation of the TCF4 network could be an important step in the biological process that leads to relapse and suggest that genes related to the ubiquitin proteosome system could be potential biomarkers of relapse. © 2021, The Author(s)

Regeneración de campus para la creación de un laboratorio vivo de sostenibilidad ("living lab") en el Campus de Excelencia Internacional de Moncloa

La Universidad Politécnica de Madrid (UPM) a través de su Centro de Innovación en Tecnología para el Desarrollo Humano (itdUPM) está propiciando la generación de conciencia, conocimiento y soluciones innovadoras que contribuyen al cumplimiento de los Objetivos de Desarrollo Sostenible a través de un edificio que sirve como laboratorio de prueba para nuevas tecnologías verdes y como plataforma de ideación colaborativa y activación social

HisAK70: Progress towards a vaccine against different forms of leishmaniosis

Background: Leishmania major and Leishmania infantum are among the main species that are responsible for cutaneous leishmaniosis (CL) and visceral leishmaniosis (VL), respectively. The leishmanioses represent the second-largest parasitic killer in the world after malaria. Recently, we succeeded in generating a plasmid DNA (pCMV-HISA70m2A) and demonstrated that immunized mice were protected against L. major challenge. The efficacy of the DNA-vaccine was further enhanced by the inclusion of KMP-11 antigen into the antibiotic-free plasmid pVAX1-asd. Methods: Here, we describe the use of a HisAK70 DNA-vaccine encoding seven Leishmania genes (H2A, H2B, H3, H4, A2, KMP11 and HSP70) for vaccination of mice to assess the induction of a resistant phenotype against VL and CL. Results: HisAK70 was successful in vaccinated mice, resulting in a high amount of efficient sterile hepatic granulomas associated with a hepatic parasite burden fully resolved in the VL model; and resulting in 100 % inhibition of parasite visceralization in the CL model. Conclusions: The results suggest that immunization with the HisAK70 DNA-vaccine may provide a rapid, suitable, and efficient vaccination strategy to confer cross-protective immunity against VL and CL.This work was partially supported by grants from the Spanish Ministry of Economy and Competitiveness (AGL2010-17394 and AGL2013-44100R) and PLATESA (P2013/ABI-2906) from the Comunidad de Madrid (Spain).Peer Reviewe