«Graphoskop», uno strumento informatico per l'analisi paleografica quantitativa

«Graphoskop» è uno strumento paleografico elaborato al fine di agevolare il lavoro critico dello storico della scrittura. Il plug-in, concepito come un'estensione del software open source «ImageJ» è in grado di rilevare dati quantitativi di tipo metrologico a partire da una rappresentazione digitale di una scrittura data. Il metodo quantitativo è quindi applicato per la valutazione complessiva di un singolo manoscritto (analisi paleografica e analisi della mise en page) e,allo stesso tempo, per la valutazione di un corpus più esteso. Il Graphoskop, infatti, esegue alcuni calcoli statistici di base sui dati raccolti e registra automaticamente tali informazioni su un foglio di calcolo. La messa a punto della metodologia quantitativa rende possibile l'utilizzo di osservazioni analitiche per studi di carattere sintetico. La procedura è quindi orientata alla ricostruzione di un panorama più ampio: identificare la nascita di un dato fenomeno e disegnarne la curva della diffusione, per potersi interrogare più chiaramente sulle cause e modalità di tale evoluzione

Characterization and dynamics of specific T cells against nucleophosmin-1 (NPM1)-mutated peptides in patients with NPM1-mutated acute myeloid leukemia

Nucleophosmin(NPM1)-mutated protein, a leukemia-specific antigen, represents an ideal target for AML immunotherapy. We investigated the dynamics of NPM1-mutated-specific T cells on PB and BM samples, collected from 31 adult NPM1-mutated AML patients throughout the disease course, and stimulated with mixtures of 18 short and long peptides (9-18mers), deriving from the complete C-terminal of the NPM1-mutated protein. Two 9-mer peptides, namely LAVEEVSLR and AVEEVSLRK (13.9-14.9), were identified as the most immunogenic epitopes. IFNγ-producing NPM1-mutated-specific T cells were observed by ELISPOT assay after stimulation with peptides 13.9-14.9 in 43/85 (50.6%) PB and 34/80 (42.5%) BM samples. An inverse correlation between MRD kinetics and anti-leukemic specific T cells was observed. Cytokine Secretion Assays allowed to predominantly and respectively identify Effector Memory and Central Memory T cells among IFNγ-producing and IL2-producing T cells. Moreover, NPM1-mutated-specific CTLs against primary leukemic blasts or PHA-blasts pulsed with different peptide pools could be expanded ex vivo from NPM1-mutated AML patients or primed in healthy donors. We describe the spontaneous appearance and persistence of NPM1-mutated-specific T cells, which may contribute to the maintenance of long-lasting remissions. Future studies are warranted to investigate the potential role of both autologous and allogeneic adoptive immunotherapy in NPM1-mutated AML patients

2020 WSES guidelines for the detection and management of bile duct injury during cholecystectomy.

Bile duct injury (BDI) is a dangerous complication of cholecystectomy, with significant postoperative sequelae for the patient in terms of morbidity, mortality, and long-term quality of life. BDIs have an estimated incidence of 0.4-1.5%, but considering the number of cholecystectomies performed worldwide, mostly by laparoscopy, surgeons must be prepared to manage this surgical challenge. Most BDIs are recognized either during the procedure or in the immediate postoperative period. However, some BDIs may be discovered later during the postoperative period, and this may translate to delayed or inappropriate treatments. Providing a specific diagnosis and a precise description of the BDI will expedite the decision-making process and increase the chance of treatment success. Subsequently, the choice and timing of the appropriate reconstructive strategy have a critical role in long-term prognosis. Currently, a wide spectrum of multidisciplinary interventions with different degrees of invasiveness is indicated for BDI management. These World Society of Emergency Surgery (WSES) guidelines have been produced following an exhaustive review of the current literature and an international expert panel discussion with the aim of providing evidence-based recommendations to facilitate and standardize the detection and management of BDIs during cholecystectomy. In particular, the 2020 WSES guidelines cover the following key aspects: (1) strategies to minimize the risk of BDI during cholecystectomy; (2) BDI rates in general surgery units and review of surgical practice; (3) how to classify, stage, and report BDI once detected; (4) how to manage an intraoperatively detected BDI; (5) indications for antibiotic treatment; (6) indications for clinical, biochemical, and imaging investigations for suspected BDI; and (7) how to manage a postoperatively detected BDI

The role of open abdomen in non-trauma patient : WSES Consensus Paper

The open abdomen (OA) is defined as intentional decision to leave the fascial edges of the abdomen un-approximated after laparotomy (laparostomy). The abdominal contents are potentially exposed and therefore must be protected with a temporary coverage, which is referred to as temporal abdominal closure (TAC). OA use remains widely debated with many specific details deserving detailed assessment and clarification. To date, in patients with intra-abdominal emergencies, the OA has not been formally endorsed for routine utilization; although, utilization is seemingly increasing. Therefore, the World Society of Emergency Surgery (WSES), Abdominal Compartment Society (WSACS) and the Donegal Research Academy united a worldwide group of experts in an international consensus conference to review and thereafter propose the basis for evidence-directed utilization of OA management in non-trauma emergency surgery and critically ill patients. In addition to utilization recommendations, questions with insufficient evidence urgently requiring future study were identified.Peer reviewe

La contabilità delle case dell’Ordine Teutonico in Puglia e in Sicilia nel Quattrocento, a cura di Kristjan Toomaspoeg. Presentazione di Hubert Houben

Gurrado Maria. La contabilità delle case dell’Ordine Teutonico in Puglia e in Sicilia nel Quattrocento, a cura di Kristjan Toomaspoeg. Presentazione di Hubert Houben. In: Bibliothèque de l'école des chartes. 2007, tome 165, livraison 2. pp. 578-579

Le carte di Léon Cadier alla Bibliothèque nationale de France. Contributo alla ricostruzione della cancelleria angioina. A cura di Serena Morelli

Gurrado Maria. Le carte di Léon Cadier alla Bibliothèque nationale de France. Contributo alla ricostruzione della cancelleria angioina. A cura di Serena Morelli. In: Bibliothèque de l'école des chartes. 2009, tome 167, livraison 2. pp. 592-594

La liturgie de Notre-Dame dans le royaume de Naples : les manuscrits de Saint-Nicolas de Bari

Livres liturgiques à l'usage de Paris dans le royaume de Naple

Régionalisme et internationalisme : problèmes de paléographie et de codicologie du Moyen Âge. Actes du XVe colloque du Comité international de paléographie latine (Vienne, 13-17 septembre 2005). Édités par Otto Kresten et Franz Lackner

Gurrado Maria. Régionalisme et internationalisme : problèmes de paléographie et de codicologie du Moyen Âge. Actes du XVe colloque du Comité international de paléographie latine (Vienne, 13-17 septembre 2005). Édités par Otto Kresten et Franz Lackner. In: Bibliothèque de l'école des chartes. 2009, tome 167, livraison 2. pp. 606-608

La contabilità delle case dell’Ordine Teutonico in Puglia e in Sicilia nel Quattrocento, a cura di Kristjan Toomaspoeg. Presentazione di Hubert Houben

Gurrado Maria. La contabilità delle case dell’Ordine Teutonico in Puglia e in Sicilia nel Quattrocento, a cura di Kristjan Toomaspoeg. Presentazione di Hubert Houben. In: Bibliothèque de l'école des chartes. 2007, tome 165, livraison 2. pp. 578-579