Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150

Aim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-transfected cells, the increased expression of NK cell-specific genes such as GZMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy

alpha-Synuclein expression and Nrf2 deficiency cooperate to aggravate protein aggregation, neuronal death and inflammation in early-stage Parkinson's disease

Although -synuclein (-SYN) aggregation is a hallmark of sporadic and familial Parkinsons disease (PD), it is not known how it contributes to early events of PD pathogenesis such as oxidative and inflammatory stress. Here, we addressed this question in a new animal model based on stereotaxic delivery of an adeno-associated viral vector (rAAV) for expression of human -SYN in the ventral midbrain of mice lacking the transcription factor Nrf2 (Nrf2(/)). Two months after surgery, Nrf2(/) mice exhibited exacerbated degeneration of nigral dopaminergic neurons and increased dystrophic dendrites, reminiscent of Lewy neurites, which correlated with impaired proteasome gene expression and activity. Dopaminergic neuron loss was associated with an increase in neuroinflammation and gliosis that were intensified in Nrf2(/) mice. In response to exogenously added -SYN, Nrf2(/) microglia failed to activate the expression of two anti-inflammatory genes, heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate quinone oxidorreductase-1 (NQO1). This impaired Nrf2 response correlated with a shift in the microglial activation profile, towards increased production of proinflammatory markers, IL-6, IL-1 and iNOS and reduced phagocytic capacity of fluorescent beads, and lower messenger RNA levels for TAM receptors Axl and Mer. Postmortem brain tissue samples from patients in early- to middle-stage progression of PD showed increased HO-1 expression in astrocytes and microglia, suggesting an attempt of the diseased brain to compensate these hallmarks of PD through activation of the Nrf2 pathway. This study demonstrates that -SYN and Nrf2 deficiency cooperate on protein aggregation, neuroinflammation and neuronal death and provides a bifactorial animal model to study early-stage PD

Lifetime determination of excited states in Cd-106

Two separate experiments using the Differential Decay Curve Method have been performed to extract mean lifetimes of excited states in 106 Cd. The inedium-spin states of interest were populated by the Mo-98(C-12, 4n) Cd-106 reaction performed at the Wright Nuclear Structure Lab., Yale University. From this experiment, two isomeric state mean lifetimes have been deduced. The low-lying states were populated by the Mo-96(C-13, 3n)Cd-106 reaction performed at the Institut fur Kernphysik, Universitat zu Koln. The mean lifetime of the I-pi = 2(1)(+) state was deduced, tentatively, as 16.4(9) ps. This value differs from the previously accepted literature value from Coulomb excitation of 10.43(9) ps

Low-energy structure of the even-A 96−104 Ru isotopes via g-factor measurements

The transient-field-perturbed angular correlation technique was used with Coulomb excitation in inverse kinematics to perform a systematic measurement of the g factors of the first excited 21+ states in the stable even-A isotopes Ru96-104. The measurements have been made relative to one another under matched kinematic conditions and include a measurement of g(21+)=+0.47(3) in Ru96

Revisiting anomalous \u3cem\u3eB\u3c/em\u3e(\u3cem\u3eE\u3c/em\u3e2;4\u3csup\u3e+\u3c/sup\u3e\u3csub\u3e1\u3c/sub\u3e→2\u3csup\u3e+\u3c/sup\u3e\u3csub\u3e1\u3c/sub\u3e)/\u3cem\u3eB\u3c/em\u3e(\u3cem\u3eE\u3c/em\u3e2;2\u3csup\u3e+\u3c/sup\u3e\u3csub\u3e1\u3c/sub\u3e→0\u3csup\u3e+\u3c/sup\u3e\u3csub\u3e1\u3c/sub\u3e) values in \u3csup\u3e98\u3c/sup\u3eRu and \u3csup\u3e180\u3c/sup\u3ePt

Recently, a set of nine nonmagic nuclei with anomalous values of the B(E2) ratio B4/2 ≡ B(E2; 4+1 → 2+1)/B(E2; 2+1 → 0+1) were identified. Such values are outside the range allowed by current collective models. In the present work, the B(E2; 4+1 → 2+1) values for two of these nuclei, 98Ru and 180Pt, were re-measured to determine if the current literature values for these nuclei are correct. 98Ru was studied in a 27Al(98Ru,98Ru∗) Coulomb excitation experiment in inverse kinematics, while the lifetime of the 4+1 state in 180Pt was measured in a 122Sn(62Ni, 4n)180Pt recoil distance method (RDM) experiment. For both nuclei, the remeasured B4/2 values are well above 1, removing the deviations from collective models

Quadrupole Moment Measurements of TSD1 and TSD2 Bands in \u3csup\u3e167\u3c/sup\u3eLu

The triaxial strongly deformed (TSD) bands in 167Lu were populated by the 123Sb(48Ca, 4n) reaction with a beam energy of 203 MeV. Gamma rays, requiring fivefold or more in prompt coincidence, were detected with the Gammasphere spectrometer. Of particular interests are TSD bands 1 and 2 which have previously been interpreted as zero phonon and one phonon wobbling bands, respectively. Using the Doppler shift attenuation method (DSAM), a preliminary transition quadrupole moment of 6.9+0.3−0.3 eb was extracted for the TSD1 band. Data analysis continues for TSD2 which is considerably more weakly populated

Measurement of Conversion Coefficients in Normal and Triaxial Strongly Deformed Bands in \u3csup\u3e167\u3c/sup\u3eLu

Internal conversion coefficients have been measured for transitions in both normal deformed and triaxial strongly deformed bands in 167Lu using the Gammasphere and ICE Ball spectrometers. The results for all in-band transitions are consistent with E2 multipolarity. Upper limits are determined for the internal conversion coefficients for linking transitions between TSD Band 2 and TSD Band 1, the nw = 1 and nw = 0 wobbling bands, respectively

Isomeric Decay of \u3csup\u3e208\u3c/sup\u3eRa

Low-energy excited states of 208Ra were investigated using the 182W(30Si, 4n) reaction at the Wright Nuclear Structure Laboratory of Yale University. Fusion evaporation recoils were selected using the gas-filled spectrometer SASSYER. Delayed γ rays, following isomeric decays, were detected at the focal plane of SASSYER with a small array of three clover Ge detectors. Transitions following a proposed J π = 8+ isomer were observed, and the half-life was measured

High-spin States in \u3csup\u3e191, 193\u3c/sup\u3eAu and \u3csup\u3e192\u3c/sup\u3ePt: Evidence for Oblate Deformation and Triaxial Shapes

High-spin states of 191, 193Au and 192Pt have been populated in the 186W(11B, xn) and 186W(11B, p4n) reactions, respectively, at a beam energy of 68 MeV and their γ decay was studied using the YRAST Ball detector array at the Wright Nuclear Structure Laboratory at Yale University. The level scheme of 193Au has been extended up to Iπ = 55/2+. New transitions were observed also in 191Au and 192Pt. Particle-plus-Triaxial-Rotor (PTR) and Total Routhian Surface (TRS) calculations were performed to determine the equilibrium deformations of the Au isotopes. The predictions for oblate deformations in these nuclei are in agreement with the experimental data. Development of nonaxial shapes is discussed within the framework of the PTR model

\u3cem\u3eg\u3c/em\u3e Factor of the 2\u3csup\u3e+\u3c/sup\u3e\u3csub\u3e1\u3c/sub\u3e State of \u3csup\u3e170\u3c/sup\u3eHf

The g factor of the 2+1 state of 170Hf was measured by perturbed γ-γ angular correlation in a static external magnetic field. The result, g(2+1) = 0.28(5), extends the systematics of g factors of even-even Hf isotopes to N = 98 and enables a better test of theoretical models. The g(2+1) experimental values of these isotopes exhibit a remarkable constancy as a function of neutron number. This phenomenon, which was also observed for other isotopic chains in the Gd–W range, is explained in terms of a recently proposed empirical model