Schiff bases attached L-glutamine and L-asparagine: First investigation on antimutagenic and antimicrobial analyses

WOS: 000336498100007PubMed ID: 23687951This study was conducted to evaluate the antimutagenic and antimicrobial activities of Schiff bases attached L-glutamine and L-asparagine. Antibacterial activities of the compounds against S. aureus, Sh. dys. typ 7, L. monocytogenes 4b, E. coli, S. typhi H, S. epidermis, Br. abortus, M. luteus, B. cereus, P. putida, and antifungal activity against Candida albicans were studied. These compounds were investigated for antimutagenic properties against Aflatoxin BI (AFBI) using micronuclei (MN) assay in human lymphocyte cell culture in vitro. The protective role of these compounds against AFBI-induced MN is probably related to its doses.Gazi University Research FundGazi University [05/2010-03]is research was supported by the Gazi University Research Fund (Project number: 05/2010-03)

Humic acids protective activity against manganese induced LTR (long terminal repeat) retrotransposon polymorphism and genomic instability effects in Zea mays

AbstractLong terminal repeat (LTR) retrotransposon polymorphism and genomic instability are considered to be one of the most important rearranging mechanisms under environmental stress. Triggering of this knowledge, we aimed to elucidate protective effect of humic acid (HA) on genomic instability and LTR retrotransposon polymorphism in Zea mays seeds subjected to manganese stress. REMAP (Retrotransposon-microsatellite Amplified Polymorphism) and IRAP (Inter-Retrotransposon Amplified Polymorphism) were used to define the GTS (Genomic Template Stability) levels and retrotransposon polymorphism. The results showed that all concentration used Mn led to an increase in retrotransposon polymorphism and DNA damage a reduction GTS rate showing the DNA damage. However, the treatments of humic acid (10%) together with Mn resulted as decreasing DNA damage and retrotransposon polymorphism and also increasing GTS. It can be suggested that HA applications removes the negative effects of Mn on retrotransposon polymorphism and GTS, when considering the research results

Melatonin: Role in Increasing Plant Tolerance in Abiotic Stress Conditions

Nowadays, due to the environmental stress factors that limit the production of crops, it has become very difficult to find suitable areas to enable the plant to reach its optimum product potential. Abiotic stress is very effective in decreasing agricultural production. Factors such as drought, salinity, high and low temperature, flood, radiation, heavy metals, oxidative stress, and nutrient deficiency can be considered as abiotic stress factors, and these sources of stress negatively affect plant growth, quality and productivity. Melatonin (MEL) was first identified in plants in 1995 and is increasingly becoming important for its role and effects in the plant system. MEL has been shown to have a substantial role in plant response to growth, reproduction, development, and different stress factors. In addition to its regulatory role, MEL also plays a protective role against different abiotic stresses such as metal toxicity, temperature, drought, and salinity. In plants, an important role of MEL is to alleviate the effects of abiotic stresses. In this review, the effects of MEL on plant growth, photosynthetic activity, metabolism, physiology, and biochemistry under abiotic stress conditions as a plant growth regulator will be examined

Determination of genetic diversity among wild grown apricots from Sakit valley in Turkey using SRAP markers

Sequence-related amplified polymorphism (SRAP) marker was employed first time to analyze genetic diversity of 57 seed propagated early-maturated wild grown apricot genotypes sampled from different parts of Sakit valley in Mediterranean Region of Turkey. Of the total 19 primer combinations investigated, 16 could amplify clearly and consistently. They produced a total of 87 fragments, of which 56 (64.3%) were polymorphic bands. All bands obtained from Me3-Em2, Me2-Em10 and Me2-Em6 primers were polymorphic. The cluster analysis revealed that the 57 genotypes were grouped into three major clusters. The similarity ratio among genotypes was between 0.73 and 0.94. There were no identical genotypes. The study revealed that SRAP marker system was useful in identification and genetic diversity analysis of wild grown apricots

Synthesis, anti-microbial and anti-mutagenic activities of some Schiff bases derivatives containing thiophene group

WOS: 000493109500021The aim of this study is to investigate for the first time in vitro antimicrobial and antimutagenic activities of Schiff bases included the azomethine group. Antimutagenic activity was evaluated by micronucleus (MN) assay. These group have been examined for antibacterial activity against pathogenic strains, Staphylococcus aureus, Escherichia coli, Salmonella typhi H, Brucella abortus, Micrococcus luteus, Bacillus cereus, Pseudomonas aeroginosa and antifungal activity against Candida albicans and Saccharomyces cerevisiae. The results of MN showed that Schiff bases ( (E)-N-(4-chlorophenyl)-1-(5-nitrothiophen-2 -yl)methanimine ; (E)-N-(2,4-dichlorophenyl)-1-(5-nitrothiophen-2-yl) methanimine) different concentrations decreased the toxic effects of Aflatoxin B-1. Especially, high concentration (20 mu M) of (E)-N-(4-chlorophenyl)-1-(5-nitrothiophen-2-yl) methanimine (compound 1) has strong antimutagenic activity. In our in vitro test systems, it was observed that Schiff bases had antimutagenic effects on human lymphocytes. On the other hand these compounds were also found to possess antimicrobial activity against some test bacteria and yeast. The antimicrobial test results of these Schiff bases included the azomethine group exhibited better activity than some known antibiotics. In particular, Compound 1 were more potent bactericides than all of the substances synthesized. In conclusion, this Schiff bases included the azomethine group can be use pharmacy industries as recognized with their noncytotoxic, antimicrobial and antimutagenic features

Ameliorating Effect of Boric Acid Against Vanadium Toxicity in Wheat (Triticum aestivum L.)

Heavy metal pollution, which is one of the most important environmental problems, has a significant effect on plant productivity. Vanadium (V) is considered one of the most important elements of the twenty-first century due to its high consumption in industries. Morphological and physiological measurements have been performed in V studies, but there are deficiencies in molecular studies. The purpose of the present work was to elucidate the effects of V on enzymes activity, DNA damage levels and genomic template stability (GTS\%) in Triticum aestivum L., as well as to investigate whether boric acid (BA) has preventive effects on these changes. Antioxidant enzyme activities were determined by SOD (superoxide dismutase), POD (peroxidase) and MDA (malondialdehyde) level. The inter-simple sequence repeats polymerase chain reaction (ISSR-PCR) assay was used to determine the genotoxic effects of V on DNA. According to the obtained results, while V stress increased MDA level and POD enzyme activity, SOD enzyme activity decreased. When V and BA were applied together, MDA level decreased and SOD and POD enzyme activities increased. All doses of V (4.4, 6.6, 8.8 mM) resulted in DNA damage and decreased GTS\%. On the other hand, different concentrations of BA (4 and 8 mM) combined with V decreased the toxic effects of V. The results suggested that V could also have negative effects on wheat plants by increasing DNA damage and lipid peroxidation, and BA has an antagonistic effect against V toxicity. BA may be an alternative to reduce genetic damage in plants

Cobalt-induced retrotransposon polymorphism and humic acid protection on maize genome

European nature conservation has a strong focus on farmland harbouring threatened species that mainly co-occur with traditional agriculture shaped way before the green revolution. Increased land-use intensity in agriculture has caused an alarming decline in farmland biodiversity during the last century. How can a landscape perspective contribute to fostering our understanding on causes and consequences of farmland biodiversity decline and improving the effectiveness of conservation measures? To answer these questions, we discuss the importance of landscape compositional and configurational heterogeneity, understanding ecological mechanisms determining how landscape structure affects farmland biodiversity and considering the interplay of farmland biodiversity and ecosystem service conservation

Humic acid effects on retrotransposon polymorphisms caused by zinc and iron in the maize (Zea mays L.) genome

Long terminal repeat (LTR) retrotransposons are important pathways to examine various genome reorganizations based on environmental factors. The maize genome is one of the best representative examples. The aim of this study was to examine humic acid (HA) protective effects on genomic template stability (GTS) and LTR retrotransposon polymorphisms in corn seeds subjected to zinc (Zn) and iron (Fe) stress. In this study, maize seedlings were exposed to three doses (20, 40, and 60 mM) of ZnSO4·7H2O and FeSO4 and their combinations at 1500 ppm (0.075 g) HA. Inter-retrotransposon amplified polymorphism and retrotransposon-microsatellite amplified polymorphism techniques were used for genetic analyses. Results indicated that in all doses used, Zn and Fe increased retrotransposon polymorphisms and decreased the percentage of GTS via DNA damage. However, treatment of HA together with Zn and Fe resulted in decreased DNA damage and retrotransposon polymorphisms and increased GTS. These observations suggest that HA can be applied to reduce toxic effects in agricultural areas polluted with heavy metals

Synthesis, antimicrobial and antimutagenic effects of novel polymeric-Schiff bases including indol

WOS: 000475344800091Herein, the synthesis and characterization of three new polymeric-Schiff bases including indol (L-1, L-2, L-3) were reported. The antibacterial and antifungal activity of all compounds were investigated by the well-diffusion method against some selected microorganisms as potential antimicrobial agents. In addition, the anti-genotoxic properties of these polymeric-Schiff bases were examined against sodium azide in human lymphocyte cells by micronuclei (MN) and sister chromatid exchange (SCE) tests. (C) 2019 Elsevier B.V. All rights reserved.Duzce UniversityDuzce University; Nevsehir Haci Bektas Veli UniversityNevsehir Haci Bektas Veli UniversityWe thank Duzce University and Nevsehir Haci Bektas Veli University for equipment funding