Neoadjuvant chemoradiation and pancreaticoduodenectomy for initially locally advanced head pancreatic adenocarcinoma

International audienceThe most accepted treatment for locally advanced pancreatic adenocarcinoma (LAPA) is chemoradiotherapy (CRT). We sought to determine the benefit of pancreaticoduodenectomy (PD) in patients with LAPA initially treated by neoadjuvant CRT

Treinador e atleta: significados de uma relação na aprendizagem e desempenho esportivo de alto rendimento à luz da teoria de Buber

A complexa relação que treinador e atleta estabelecem ao longo dos anos de convívio está permeada por múltiplos sentimentos, significados e emoções. Atravessada por momentos de exigência de altos graus de perfeição, produtividade e competência e, ao mesmo tempo, por uma profunda conexão entre o treinador e o atleta, esta relação está fundamentada por um misto de sentimentos que oscilam entre o afeto, a raiva, a admiração, a confiança e até mesmo a decepção. Procurando debater sobre importantes elementos que transitam por este relacionamento, a relação treinador-atleta é examinada à luz da teoria de Buber, que entende a existência dialógica do Eu-Tu como o fundamento das relações humanas, mas que também considera a relação Eu-Isso como o lugar e o suporte da experiência, do conhecimento e da utilização. O objetivo geral deste estudo é analisar o significado da relação treinador-atleta na aprendizagem e proficiência esportiva do atleta de alto rendimento de modalidades esportivas individuais sob a ótica do Eu-Tu e Eu-Isso de Buber. Como objetivos específicos a pesquisa se propõe a avaliar como os desdobramentos do Eu-Tu e do Eu-Isso na relação treinador-atleta refletem na aprendizagem e no desempenho esportivo de alto rendimento de modalidades esportivas individuais e identificar quais os indicadores de aprendizagem e desempenho que emergem do Eu-Tu e do Eu-Isso na relação treinador-atleta em modalidades esportivas individuais.Como instrumento de coleta de dados foi utilizada a entrevista semi-estruturada com atletas de alto nível de desempenho de modalidades esportivas individuais e seus respectivos treinadores, usando como método de análise dos resultados a análise textual discursiva. A análise dos dados obtidos por meio das entrevistas mostrou que elementos do Eu-Tu e do Eu-Isso estão em permanente movimento de ir e vir, permeando a relação treinador-atleta. Assim, reciprocidade, encontro, confiança e suporte são as principais características encontradas na relação treinador-atleta no modo Eu-Tu, enquanto objetividade, utilização e distanciamento são as características reveladas no modo Eu-Isso, influenciando diretamente a aprendizagem e o desempenho esportivo.The complex relationship that coaches and athletes establish throughout years together is permeated by multiple feelings, meanings, and emotions. It is crossed by moments that demand high levels of perfection, productivity, competence and, at the same time, a deep connection between both sides, turning this relationship into a mixture of feelings that oscillate between affection, anger, admiration, confidence and even disappointment. Aiming to discuss important elements that move along this relationship, the coach-athlete relationship was examined through the eyes of Buber's theory, which understands the dialogic existence of the I-Thou as the foundation of human relations and the I-It as the environment and support of experience, knowledge and application. This way, the general objective of this study is to analyze the significance of the coach-athlete relationship in the learning and athletic proficiency of high performance athletes of individual sports modalities from the perspective of Buber's I-Thou and I-It theory. The specific objectives of this research are: to evaluate how the I-Thou and I-It theory, unfolded in the coach-athlete relationship, reflects on the high-performance learning and performance of individual sports modalities and to identify which learning and performance indicators emerge from the I-Thou and the I-It theory in the coach-athlete relationship in individual sports modalities. A semi-structured interview was used as the collection instrument of the study and applied on high performance athletes of individual sports modalities and their respective coaches, using as method of analysis of the results the discursive textual analysis.The data analysis of the interviews revealed that the I-Thou and the I-It elements shift constantly, permeating the coach-athlete relationship. Therefore, reciprocity, gathering, confidence, and support are the main characteristics found in the I-Thou relationship, while objectivity, utility, and distance are the characteristics found in I-It, having a direct influence upon learning and sports performance

Développement de systèmes d’imagerie dans la gamme des fréquences térahertz

Les ondes térahertz se situent dans le spectre électromagnétique, entre les rayonnements microondes et infrarouges. De par leurs propriétés intéressantes, elles présentent un intérêt croissant depuis quelques dizaines d’années autant dans le monde de la recherche fondamentale que dans le secteur industriel. De plus, le développement de nombreuses techniques durant ces dernières années pour générer et détecter ces ondes, a ouvert à la porte à de multiples applications. Bon nombre d’entre elles sont basées sur l’acquisition d’images d’objets et repose donc sur l’utilisation de systèmes d’imagerie. Le temps nécessaire à la prise d’une image, la sensibilité de détection ainsi que la résolution spatiale sont des paramètres importants à considérer en imagerie THz. L’objectif principal de ce travail de thèse expérimentale est de développer des systèmes d’imagerie dans la gamme de fréquences térahertz. Ces derniers permettent l’acquisition rapide d’images, avec une sensibilité élevée et une résolution spatiale optimale. Des contributions ont été apportées, de la source de lumière THz utilisée pour illuminer l’objet imagé jusqu’à la technique d’imagerie employée et le traitement des images. Ce manuscrit est structuré entre une mise en contexte détaillé du projet de recherche et une présentation complète des résultats obtenus. Le domaine des ondes térahertz est tout d’abord introduit dans une première partie (voir Introduction). En plus de ses applications, les principales méthodes utilisées pour émettre et détecter ces ondes sont présentées. Les motivations et les objectifs de ce travail y sont également décrits. Ensuite, une seconde partie (voir Chapitre 1) expose l’état de l’art autour des concepts clés évoqués dans ce travail. Par la suite, une troisième partie (voir Chapitre 2 ) présente l’étude qui a été réalisée sur une source THz par redressement optique d’une impulsion laser à ytterbium dans un cristal de tellurure de zinc. Une quatrième partie (voir Chapitre 3) présente une source THz basée sur le redressement optique dans un cristal niobate de lithium grâce à une technique de pompage avec un front d’onde incliné. C’est cette source très performante qui a été utilisée avec les systèmes d’imagerie mis en place dans la suite de ce travail de thèse. Une cinquième partie décrit un système d’imagerie cohérent utilisé dans une configuration en champ proche (voir Chapitre 4). Avec ce dernier, nous avons démontré l’acquisition d’images hyperspectrales avec des résolutions sub-longueur d’onde. Une sixième partie se concentre sur le travail exploratoire mené sur une technique d’imagerie permettant la reconstruction d’images THz avec une résolution spatiale améliorée (voir Chapitre 5). Avec cette dernière, une technique de modulation active d’un faisceau THz a notamment été mise en place. Enfin, une dernière partie présente les perspectives envisagées émanant de ce travail

Alternative splicing of the dopamine D2 receptor directs specificity of coupling to G-proteins.

International audienceTwo isoforms of the dopamine D2 receptor have been characterized, D2L (long) and D2S (short), generated by alternative splicing from the same gene. They differ by an in-frame insert of 29 amino acids specific to D2L within the putative third intracytoplasmic loop of the receptor. We have previously demonstrated (Montmayeur, J.-P., Guiramand, J., and Borelli, E. (1993) Mol. Endocrinol. 7, 161-170) that D2S and D2L, although presenting very similar pharmacological profiles, couple differently to the alpha-subunit of guanine nucleotide-binding regulatory proteins (G-proteins). In particular, D2L, but not D2S, requires the presence of the alpha-subunit of the inhibitory G-protein (G alpha i2) to elicit greater inhibition of adenylyl cyclase activity. The insert present in D2L must therefore confer the specificity of interaction with G alpha i2. Thus, we introduced substitution mutations within the D2L insert. These mutant receptors were expressed in JEG3 cells, a G alpha i2-deficient cell line, scoring for those presenting an increased inhibition of adenylyl cyclase by dopamine. Our analysis identified two mutants, S259/262A and D249V, with these properties. These results clearly show that the insert present in D2L plays a critical role in the selectivity for the G-proteins interacting with the receptor

Identidade brasileira em transição: O olhar da mídia estrangeira sobre a cerimônia de abertura dos Jogos Olímpicos Rio 2016

The article aims at an investigation of the forms of narration of Brazil’s international image through the Olympic Games Rio 2016. The objective is to extend the knowledge and understanding of narratives upon Brazil and its identity elements which were built abroad in the context of the Olympic Games Rio 2016. Texts from published international newspapers are analyzed as result of the Opening Ceremony. The selection criterion of the periodicals was defined from the participating countries whose official languages were Portuguese, Spanish and or English. The study characterizes itself as a qualitative research, based on bibliographic and documental sources. The analysis showed new identity representations about Brazil, pointing to the presence of new elements, from the reports published by foreign media.O artigo tem como objetivo uma investigação sobre as formas de narração da imagem internacional do Brasil através dos Jogos Olímpicos do Rio 2016. O objetivo é ampliar o conhecimento e a compreensão sobre narrativas sobre o Brasil e seus elementos identitários construídos no exterior no contexto dos Jogos Olímpicos do Rio 2016. São analisados os textos de jornais internacionais publicados como decorrência da Cerimônias de Abertura. O critério de escolha dos periódicos foi definido a partir dos países participantes, que tivessem como idioma português, espanhol ou inglês. O estudo caracteriza-se como uma pesquisa qualitativa, baseada em fontes bibliográficas e documentais. A análise mostrou novas representações identitárias sobre o Brasil, apontando para a presença de novos elementos, a partir das reportagens divulgadas pela mídia estrangeira

Solitary Fibrous Tumor in the Round Ligament of the Liver: A Fortunate Intraoperative Discovery

Solitary fibrous tumors (SFTs) are mesenchymal neoplasms of fibroblastic origin, most commonly found in the pleura. Numerous extrathoracic locations have been reported during the last 2 decades. Herein, we report the first case of an SFT in the round ligament of the liver. A 46-year-old Caucasian man presented with a 12-month history of abdominal pain. An ultrasonography-guided microbiopsy first revealed a desmoid tumor. After failure of first- and second-line medical treatments (celecoxib and tamoxifen, then imatinib), histological reexamination was suspicious for a low-grade sarcoma. MRI was also suspicious for a malignant process. Hence, surgery was decided. Laparotomy found a huge and well-limited tumor that, unexpectedly, was appended to the round ligament of the liver and free from any other intra-abdominal contact. The tumor was easily removed. Excision was monobloc and macroscopically complete. Histological analysis diagnosed an SFT arising from the round ligament of the liver. No adjuvant treatment was given. Ten months after surgery, the patient is alive without any signs or symptoms of relapse. This is the first report of SFT arising from the round ligament of the liver. It illustrates the difficulty in diagnosing such tumors. Whilst diagnosis of SFT is rare, it should be kept in mind to allow early diagnosis and complete surgical resection, which provide the best chance for recovery

Primary leiomyosarcoma of the seminal vesicle: Case report and review of the literature

International audienceAbstract Background Primary leiomyosarcoma of the seminal vesicle is exceedingly rare. Case Presentation We report a case of a 59-year-old man with tumour detected by rectal symptoms and ultrasonography. Computed tomography and magnetic resonance imaging suggested an origin in the right seminal vesicle. Transperineal biopsy of the tumour revealed leiomyosarcoma. A radical vesiculo-prostactectomy with bilateral pelvic lymphadenectomy was performed. Pathological examination showed a grade 2 leiomyosarcoma of the seminal vesicle. The patient received adjuvant radiotherapy. He developed distant metastases 29 months after diagnosis, and received chemotherapy. Metastatic disease was controlled by second-line gemcitabine-docetaxel combination. Fifty-one months after diagnosis of the primary tumour, and 22 months after the first metastases, the patient is alive with excellent performance status, and multiple asymptomatic stable lung and liver lesions. Conclusions We report the eighth case of primary leiomyosarcoma of the seminal vesicle and the first one with a so long follow-up

Cholinergic regulation of the phosphoinositide second messenger system in the guinea pig organ of Corti

The effect of cholinergic agents on the phosphoinositide second messenger system was investigated in the cochlea of the adult guinea pig in vivo and in vitro. In vivo, phospholipids were labeled with [32P]-orthophosphate by perilymphatic perfusion and their hydrolysis assayed in llschase' experiments with non-radioactive orthophosphate. Carbachol (1 mM) reduced the content of 32P-labeled phosphatidylinositol 4,5-bisphosphate in the organ of Corti from 31% to 21% of total 32P-lipids, indicating stimulated hydrolysis. The pharmacology of this effect was studied in detail in vitro via the release of inositol phosphates from phosphoinositides pre-labeled with 3H-inositol. Release was increased 2-fold by 1 mM carbachol, 1.6-fold by 1 mM muscarine, but was unaffected by dimethylphenylpiperazinium; the stimulation was blocked by 1 [mu]M atropine but not mecamylamine. These responses indicate the coupling of phosphoinositides to a muscarinic receptor. Furthermore. stimulated inositol phosphate release was higher in the base of the organ of Corti than in the apex which correlates with the increased cholinergic efferent innervation of outer hair cells in the basal region. These results suggest that muscarinic-stimulated inositol phosphate release occurs at the level of the outer hair cell and thus may have an important modulatory role in auditory transduction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/30076/1/0000446.pd

G-proteins coupled to phosphoinositide hydrolysis in the cochlear and vestibular sensory epithelia of the rat are insensitive to cholera and pertussis toxins

In the cochlear (CSE) and vestibular sensory epithelia (VSE), phosphoinositides are hydrolyzed in response to stimulation of phospholipase C (PLC) by cholinergic muscarinic and purinergic P2y agonists. Such receptor-mediated activation of PLC is expected to be coupled through guanine nucleotide-binding proteins (G-proteins). Although several classes of G-proteins have been identified in the inner ear, nothing is known about the type of G-proteins associated with the phosphoinositide second messenger system in CSE and VSE. Phosphoinositide hydrolysis was determined by the release of radiolabeled inositol phosphates (InsPs). Ten mM NaF plus 10 [mu]M AlCl3 increased basal InsPs accumulation 2-fold in both CSE and VSE of the rat. Release of InsPs was also enhanced by guanosine 5'-O-(3-thiotriphosphate) (GTP-[gamma]-S) in saponin-permeabilized tissues. Furthermore, release of InsPs stimulated by both carbamylcholine (CCh) and adenosine 5'-O-[3-thiotriphosphate](ATP-[gamma]-S) was significantly inhibited by 100 [mu]M guanosine 5'-O-[2-thiodiphosphate](GDP-[beta]-S). These results strongly suggest the involvement of G-proteins in the receptor-PLC coupling in CSE and VSE. ADP-ribosylation in membrane fractions of CSE and VSE in the presence of cholera toxin (CTX) or pertussis toxin (PTX) indicated the existence of Gs- and Gi-type G-proteins. However, neither CTX nor PTX affected basal or agonist-stimulated release of InsPs. These observations suggest that muscarinic and P2y purinergic receptors are coupled to PLC via CTX- and PTX-insensitive G-proteins in CSE and VSE.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/31661/1/0000595.pd