Medaka piwi is Essential for Primordial Germ Cell Migration

Piwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration.Piwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration

Interactions between a magnon mode and a cavity photon mode mediated by traveling photons

We systematically study the indirect interaction between a magnon mode and a cavity photon mode mediated by traveling photons of a waveguide. From a general Hamiltonian, we derive the effective coupling strength between two separated modes, and obtain the theoretical expression of the system's transmission. Accordingly, we design an experimental setup consisting of a shield cavity photon mode, a microstrip line, and a magnon system to test our theoretical predictions. From measured transmission spectra, indirect interaction, as well as mode hybridization, between two modes can be observed. All experimental observations support our theoretical predictions. In this work we clarify the mechanism of traveling photon mediated interactions between two separate modes. Even without spatial mode overlap, two separated modes can still couple with each other through their correlated dissipations into a mutual traveling photon bus. This conclusion may help us understand the recently discovered dissipative coupling effect in cavity magnonics systems. Additionally, the physics and technique developed in this work may benefit us in designing new hybrid systems based on the waveguide magnonics.QN/Bauer Grou

Coherent control of magnon radiative damping with local photon states

A magnon, the collective excitation of ordered spins, can spontaneously radiate a travelling photon to an open system when decaying to the ground state. However, in contrast to electric dipoles, magnetic dipoles by magnons are more isolated from the environment, limiting their radiation and coherent communication with photons. The recent progresses in strongly coupled magnon-photon system have stimulated the manipulation of magnon radiation via tailoring the photon states. Here, by loading an yttrium iron garnet sphere in a one-dimensional waveguide cavity supporting both the travelling and standing photon modes, we demonstrate a significant magnon radiative damping that is proportional to the local density of photon states (LDOS). By modulating the magnitude and/or polarization of LDOS, we can flexibly tune the photon emission and magnon radiative damping. Our findings provide a way to manipulate photon emission from magnon radiation, which could help harness angular momentum generation, transfer, and storage in magnonics.QN/Bauer Grou

Measurement of charged particle multiplicities and densities in pp collisions at s√=7 TeV in the forward region

Charged particle multiplicities are studied in proton–proton collisions in the forward region at a centre-ofmass energy of √ s = 7 TeV with data collected by the LHCb detector. The forward spectrometer allows access to a kinematic range of 2.0 < η < 4.8 in pseudorapidity, momenta greater than 2 GeV/c and transverse momenta greater than 0.2 GeV/c. The measurements are performed using events with at least one charged particle in the kinematic acceptance. The results are presented as functions of pseudorapidity and transverse momentum and are compared to predictions from several Monte Carlo event generators