Species and generic delimitation in Bikinia and Tetraberlinia

We have assessed the monophyly and internal topology of Bikinia and Tetraberlinia along with some other systematic and methodological questions using AFLP analyses. In addition to AFLP analyses we tried to determine the sister group of these two genera by adding additional sequences to an existing ITS data set. Our analyses suggest Julbernardia is the closest related genus, but also Icuria is a candidate since the position of this genus remains unclear. Although ITS provides us with some good resolution at the generic level, we have to conclude ITS is not a good marker in this group due to the presence of several non-homologous copies. Evidence for a monophyletic Bikinia is quite strong. However, more evidence is needed to test the monophyly of Tetraberlinia. Within Bikinia, only a clade consisting of B. aciculifera and B. durandii was supported by a jackknife analysis. We show that B. le-testui and B. pellegrinii are separate species. Aberrant Bikinia material from the Crystal Mountains in Gabon proved to be a new species. A sapling collected under a tree of B. le-testui could be identified as a hybrid between this species and B. media. Another new species, T. apiphila, is clearly related to Tetraberlinia, although it also shares some morphological characters with Bikinia. We demonstrated that AFLP results can be reproduced and that the errors of such replications fall within the variation present at the population level. AFLP can discriminate between different populations of a single species, even with high jackknife support. In the sample of genera studied here the AFLP technique provides high resolution at generic level and we even expect it to work between several closely related genera. Finally, we describe how the AFLP technique can be used to identify hybrids

Solar limb darkening function and solar diameter with eclipses observations

We introduce a new method to perform high resolution astrometry of the solar diameter from the ground, through the observations of eclipses. A discussion of the solar diameter and its variations is linked to the Limb Darkening Function (LDF) using the luminosity evolution of a Baily's Bead and the profile of the lunar limb available from satellite data. The inflexion point of the LDF is defined as the solar limb. The method proposed is applied for the videos of the eclipse in January, 15, 2010 recorded by Richard Nugent in Uganda and Andreas Tegtmeier in India. An upper limit for the inflexion point position has been set for that eclipse.Comment: 3 pages, 2 figures. Proceedings of the Fourth French-Chinese meeting on Solar Physics Understanding Solar Activity: Advances and Challenges, 15 - 18 November, 2011 Nice, Franc

Basal autophagy is involved in the degradation of the ERAD component EDEM1

Abstract.: Little is known about the fate of machinery proteins of the protein quality control and endoplasmic reticulum(ER)-associated degradation (ERAD). We investigated the degradation of the ERAD component EDEM1, which directs overexpressed misfolded glycoproteins to degradation. Endogenous EDEM1 was studied since EDEM1 overexpression not only resulted in inappropriate occurrence throughout the ER but also caused cytotoxic effects. Proteasome inhibitors had no effect on the clearance of endogenous EDEM1 in non-starved cells. However, EDEM1 could be detected by immunocytochemistry in autophagosomes and biochemically in LC3 immuno-purified autophagosomes. Furthermore, influencing the lysosome-autophagy pathway by vinblastine or pepstatin A/E64d and inhibiting autophagosome formation by 3-methyladenine or ATGs short interfering RNA knockdown stabilized EDEM1. Autophagic degradation involved removal of cytosolic Triton X-100-insoluble deglycosylated EDEM1, but not of EDEM1-containing ER cisternae. Our studies demonstrate that endogenous EDEM1 in cells not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophag

MRGPRX2 Is the Codeine Receptor of Human Skin Mast Cells: Desensitization through β-Arrestin and Lack of Correlation with the FcεRI Pathway

Codeine stimulates skin mast cells and is therefore used in skin tests and as an inducer of experimental itch.MRGPRX2 responds to various drugs, including opioids, to elicit pseudoallergic reactions, but whether it represents the main opiate receptor of skin mast cells remains unknown. By combining a number of approaches, including the silencing of MRGPRX2, we now report that MRGPRX2 is indeed the dominant codeine receptor of dermal mast cells. Activation by codeine displayed profound subject variability and correlated with secretion elicited by compound 48/80 or substance P but not by FcεRI aggregation. Degranulation by codeine was attenuated by stem cell factor, whereas the opposite was found for FcεRI. Compound 48/80 or codeinealone was able to achieve maximum MRGPRX2 activation. MRGPRX2 was rapidly internalized on codeine binding in a b-arrestin-1‒dependent manner. Codeine-triggered b-arrestin activation was also established by the Tango assay. Prestimulation with MRGPRX2 agonists (but not C3a or FcεRI aggregation) resulted in refractoriness to further stimulation by the same or another MRGPRX2 ligand (cross desensitization). This was duplicated in a cell line (RBL-MRGPRX2). Collectively, codeine degranulates skin mast cells through MRGPRX2, at which it acts as a balanced ligand. It has yet to be determined whether codeine-induced refractoriness could be exploited to desensitize MRGPRX2 to prevent severe pseudoallergic reactions

Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition.

BACKGROUND: Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV, and TcVI are associated with severe human disease in the Southern Cone countries, whereas TcI is associated with cardiomyopathy north of the Amazon. T. cruzi persists as a chronic infection, with cardiac and/or gastrointestinal symptoms developing years or decades after initial infection. Identifying an individual's history of T. cruzi lineage infection directly by genotyping of the parasite is complicated by the low parasitaemia and sequestration in the host tissues. METHODOLOGY/PRINCIPAL FINDINGS: We have applied here serology against lineage-specific epitopes of the T. cruzi surface antigen TSSA, as an indirect approach to allow identification of infecting lineage. Chagasic sera from chronic patients from a range of endemic countries were tested by ELISA against synthetic peptides representing lineage-specific TSSA epitopes bound to avidin-coated ELISA plates via a biotin labelled polyethylene glycol-glycine spacer to increase rotation and ensure each amino acid side chain could freely interact with their antibodies. 79/113 (70%) of samples from Brazil, Bolivia, and Argentina recognised the TSSA epitope common to lineages TcII/TcV/TcVI. Comparison with clinical information showed that a higher proportion of Brazilian TSSApep-II/V/VI responders had ECG abnormalities than non-responders (38% vs 17%; p<0.0001). Among northern chagasic sera 4/20 (20%) from Ecuador reacted with this peptide; 1/12 Venezuelan and 1/34 Colombian samples reacted with TSSApep-IV. In addition, a proposed TcI-specific epitope, described elsewhere, was demonstrated here to be highly conserved across lineages and therefore not applicable to lineage-specific serology. CONCLUSIONS/SIGNIFICANCE: These results demonstrate the considerable potential for synthetic peptide serology to investigate the infection history of individuals, geographical and clinical associations of T. cruzi lineages

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets

The Measurement of Solar Diameter and Limb Darkening Function with the Eclipse Observations

The Total Solar Irradiance varies over a solar cycle of 11 years and maybe over cycles with longer period. Is the solar diameter variable over time too? We introduce a new method to perform high resolution astrometry of the solar diameter from the ground, through the observations of eclipses by reconsidering the definition of the solar edge. A discussion of the solar diameter and its variations must be linked to the Limb Darkening Function (LDF) using the luminosity evolution of a Baily's Bead and the profile of the lunar limb available from satellite data. This approach unifies the definition of solar edge with LDF inflection point for eclipses and drift-scan or heliometric methods. The method proposed is applied for the videos of the eclipse in 15 January 2010 recorded in Uganda and in India. The result shows light at least 0.85 arcsec beyond the inflection point, and this suggests to reconsider the evaluations of the historical eclipses made with naked eye.Comment: 16 pages, 11 figures, accepted in Solar Physics. arXiv admin note: text overlap with arXiv:astro-ph/0601109 by other author